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The map label for this gene is lysS [H]

Identifier: 148266976

GI number: 148266976

Start: 582662

End: 584209

Strand: Direct

Name: lysS [H]

Synonym: SaurJH9_0539

Alternate gene names: 148266976

Gene position: 582662-584209 (Clockwise)

Preceding gene: 148266975

Following gene: 148266978

Centisome position: 20.05

GC content: 34.88

Gene sequence:

>1548_bases
ATGTTTGAGTGTACCAGTTGGAATACTAAAGGATTACAACAAGTTAAAGGAGAGAAAGTTATGTCAGAAGAAATGAATGA
CCAAATGTTGGTTCGACGTCAAAAATTACAAGAATTATATGATCTTGGTATAGACCCGTTTGGTTCTAAATTTGACCGTT
CAGGTTTATCTAGTGATTTGAAAGAAGAGTGGGACCAGTATTCTAAAGAAGAATTGGTAGAAAAAGAAGCGGATAGTCAT
GTCGCTATAGCTGGACGATTAATGACTAAGCGTGGTAAAGGTAAAGCAGGATTTGCACACGTTCAGGACTTAGCTGGACA
AATTCAAATTTACGTTCGTAAAGATCAAGTTGGCGATGACGAATTTGATTTATGGAAAAATGCTGATTTAGGCGATATCG
TTGGTGTTGAAGGTGTAATGTTCAAAACAAATACTGGCGAATTATCGGTTAAAGCGAAGAAATTCACGCTACTAACTAAA
TCATTGCGACCATTACCGGATAAATTCCACGGTTTACAGGATATTGAACAGAGATATCGTCAAAGATATTTAGATTTAAT
TACGAACGAAGATAGCACTCGTACATTTATTAATCGTAGTAAAATCATTCAAGAAATGCGTAATTATTTAAATAATAAAG
GTTTCTTGGAAGTAGAAACACCTATGATGCACCAAATTGCTGGTGGCGCAGCTGCTAGACCATTTGTAACACATCATAAT
GCATTAGATGCAACGTTATACATGCGTATTGCTATTGAGTTGCATTTAAAACGTTTAATTGTCGGTGGACTTGAAAAAGT
ATATGAAATTGGTAGAGTATTCCGTAATGAAGGTGTATCAACTAGACATAACCCTGAATTCACAATGATTGAATTATATG
AAGCATATGCAGATTATCATGACATTATGGATTTAACAGAATCTATGGTGAGACATATTGCCAATGAAGTATTAGGTTCT
GCAAAAGTACAATACAATGGGGAAACGATTGATTTAGAATCTGCTTGGACTCGTTTGCATATTGTTGATGCTGTAAAAGA
AGCTACTGGTGTGGATTTTTATGAAGTTAAAAGTGATGAAGAAGCTAAAGCTTTAGCTAAAGAACATGGTATTGAAATTA
AAGATACAATGAAATATGGTCATATTTTAAATGAATTCTTTGAGCAAAAAGTTGAAGAAACACTTATTCAGCCAACGTTT
ATCTATGGTCATCCGACTGAAATTTCACCTTTAGCGAAGAAAAATCCTGAAGATCCTAGATTTACTGATCGTTTCGAATT
GTTCATTGTAGGTAGAGAGCATGCAAATGCATTTACTGAATTAAATGATCCTATTGATCAAAAAGGTCGTTTTGAAGCGC
AACTTGTTGAAAAGGCGCAAGGTAATGATGAAGCGCATGAAATGGATGAAGATTACATTGAAGCGTTAGAATATGGTATG
CCTCCGACAGGTGGTCTTGGTATCGGTATTGACAGATTGGTTATGTTATTAACTGATTCTCCATCAATCAGAGACGTGTT
ATTATTCCCTTATATGAGACAAAAATAA

Upstream 100 bases:

>100_bases
TAACTTTAAGGTGTAATGGTTAATGATAAAGTATTAGAAACATCGAAAGAGATGTATGTTGAGCAAAAATGTCTGATATT
TTATAAAACTTTAAAGGAAA

Downstream 100 bases:

>100_bases
ATGACGTTGATTGTTAGTAAGAGCTCTCGTGTATACAACATGTGTATGCGAGGGTTTTCTTAATTATGGTAATTAGTTCG
TGTTTGAATGTTTTTGATAG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MFECTSWNTKGLQQVKGEKVMSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSH
VAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTK
SLRPLPDKFHGLQDIEQRYRQRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHN
ALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGS
AKVQYNGETIDLESAWTRLHIVDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTF
IYGHPTEISPLAKKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGM
PPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK

Sequences:

>Translated_515_residues
MFECTSWNTKGLQQVKGEKVMSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSH
VAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTK
SLRPLPDKFHGLQDIEQRYRQRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHN
ALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGS
AKVQYNGETIDLESAWTRLHIVDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTF
IYGHPTEISPLAKKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGM
PPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK
>Mature_515_residues
MFECTSWNTKGLQQVKGEKVMSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSH
VAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTK
SLRPLPDKFHGLQDIEQRYRQRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHN
ALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGS
AKVQYNGETIDLESAWTRLHIVDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTF
IYGHPTEISPLAKKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGM
PPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=508, Percent_Identity=42.5196850393701, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI194272210, Length=508, Percent_Identity=42.5196850393701, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI45439306, Length=497, Percent_Identity=24.1448692152917, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI40789249, Length=255, Percent_Identity=25.4901960784314, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1790571, Length=497, Percent_Identity=53.1187122736419, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1789256, Length=495, Percent_Identity=52.7272727272727, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87082379, Length=327, Percent_Identity=31.4984709480122, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788173, Length=309, Percent_Identity=23.9482200647249, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=488, Percent_Identity=44.4672131147541, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535925, Length=488, Percent_Identity=44.4672131147541, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71994340, Length=488, Percent_Identity=44.4672131147541, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551876, Length=484, Percent_Identity=23.1404958677686, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=284, Percent_Identity=24.6478873239437, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=511, Percent_Identity=43.4442270058708, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324256, Length=476, Percent_Identity=32.7731092436975, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=476, Percent_Identity=24.5798319327731, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321807, Length=464, Percent_Identity=23.9224137931034, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=137, Percent_Identity=32.1167883211679, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640851, Length=524, Percent_Identity=41.9847328244275, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640849, Length=524, Percent_Identity=41.9847328244275, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17136276, Length=484, Percent_Identity=23.7603305785124, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24584738, Length=255, Percent_Identity=28.6274509803922, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 59016; Mature: 59016

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFECTSWNTKGLQQVKGEKVMSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDL
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHH
KEEWDQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDD
HHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCCCHHHHHHHCCEEEEEEECCCCCCC
EFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
HHHHCCCCCCCCCCCCCCEEEECCCCCEEEEHHHHHHHHHHCCCCCHHHCCHHHHHHHHH
QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHN
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCCCCCCCCC
ALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
DIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFYEVKSDE
HHHHHHHHHHHHHHHHHHCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCEEEECCCH
EAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLAKKNPEDPR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHCCCCCCC
FTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGM
CCCEEEEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHCC
PPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK
CCCCCCCCCHHHHHHHHCCCCCCCHHHCCCHHCCC
>Mature Secondary Structure
MFECTSWNTKGLQQVKGEKVMSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDL
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHH
KEEWDQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDD
HHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCCCHHHHHHHCCEEEEEEECCCCCCC
EFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
HHHHCCCCCCCCCCCCCCEEEECCCCCEEEEHHHHHHHHHHCCCCCHHHCCHHHHHHHHH
QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHN
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCCCCCCCCC
ALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
DIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFYEVKSDE
HHHHHHHHHHHHHHHHHHCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCEEEECCCH
EAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLAKKNPEDPR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHCCCCCCC
FTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGM
CCCEEEEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHCC
PPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK
CCCCCCCCCHHHHHHHHCCCCCCCHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA