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The map label for this gene is metG [H]

Identifier: 148266948

GI number: 148266948

Start: 554339

End: 556312

Strand: Direct

Name: metG [H]

Synonym: SaurJH9_0511

Alternate gene names: 148266948

Gene position: 554339-556312 (Clockwise)

Preceding gene: 148266947

Following gene: 148266949

Centisome position: 19.07

GC content: 34.09

Gene sequence:

>1974_bases
ATGGCTAAAGAAACATTTTATATAACAACCCCAATATACTATCCTAGTGGGAATTTACATATAGGACATGCATATTCTAC
AGTGGCTGGAGATGTTATTGCAAGATATAAGAGAATGCAAGGATATGATGTTCGTTATTTGACTGGAACGGATGAACACG
GTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAA
CAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGAACAACTGAAGAACGTCATAAACATGTCGTTGAGCA
AGTGTTTGAACGTTTATTAAAGCAAGGTGATATCTATTTAGGTGAATATGAAGGTTGGTATTCTGTTCCGGATGAAACAT
ACTATACAGAGTCACAATTAGTAGACCCACAATACGAAAACGGTAAAATTATTGGTGGCAAAAGTCCAGATTCTGGACAC
GAAGTTGAACTAGTTAAAGAAGAAAGTTATTTCTTTAATATTAGTAAATATACAGACCGTTTATTAGAGTTCTATGACCA
AAATCCAGATTTTATACAACCACCATCAAGAAAAAATGAAATGATTAACAACTTCATTAAACCAGGACTTGCTGATTTAG
CTGTTTCTCGTACATCATTTAACTGGGGTGTCCATGTTCCGTCTAATCCAAAACATGTTGTTTATGTTTGGATTGATGCG
TTAGTTAACTATATTTCAGCATTAGGCTATTTATCAGATGATGAGTCACTATTTAACAGATACTGGCCAGCAGATATTCA
TTTAATGGCTAAGGAAATTGTGCGATTCCACTCAATTATTTGGCCTATTTTATTGATGGCATTAGACTTACCGTTACCTA
AAAAAGTCTTTGCACATGGTTGGATTTTGATGAAAGATGGAAAAATGAGTAAATCTAAAGGTAATGTTGTAGACCCTAAT
ATTTTAATTGATCGCTATGGTTTAGATGCTACACGTTATTATCTAATGCGTGAATTACCATTTGGTTCAGATGGCGTATT
TACACCTGAAGCATTTGTTGAGCGTACAAATTTCGATCTAGCAAATGACTTAGGTAACTTAGTAAACCGTACGATTTCTA
TGGTTAATAAGTACTTTGATGGCGAATTACCAGCGTATCAAGGTCCACTTCATGAATTAGATGAAGAAATGGAAGCTATG
GCTTTAGAAACAGTGAAAAGCTACACTGAAAGCATGGAAAGTTTGCAATTTTCTGTGGCATTATCTACGGTATGGAAGTT
TATAAGTAGAACGAATAAGTATATTGACGAAACAACGCCTTGGGTATTAGCTAAGGACGATAGCCAAAAAGATATGTTAG
GCAATGTAATGGCTCACTTAGTTGAAAATATTCGTTATGCAGCTGTATTATTACGTCCATTCTTAACACATGCGCCGAAA
GAGATTTTTGAACAATTGAACATAAACAATCCTCAATTTATGGAATTTAGTAGTTTAGAGCAATATGGTGTGCTTACTGA
GTCAATTATGGTTACTGGGCAACCTAAACCTATTTTCCCAAGATTGGATAGCGAAGCGGAAATTGCATATATCAAAGAAT
CAATGCAACCGCCTGCTACTGAAGAGGAAAAAGAAGAGATTCCTAGCAAACCTCAAATTGATATTAAAGACTTTGATAAA
GTTGAAATTAAGGCAGCAACGATTATTGATGCTGAACATGTTAAGAAGTCAGATAAGCTTTTAAAAATTCAAGTAGACTT
AGATTCTGAACAAAGACAAATTGTATCAGGAATTGCCAAATTCTATACACCAGATGATATTATTGGTAAAAAAGTAGCAG
TTGTTACTAACCTGAAACCAGCTAAATTAATGGGACAAAAATCTGAAGGTATGATATTATCTGCTGAAAAAGATGGTGTA
TTAACCTTAGTAAGTTTACCAAGTGCAATTCCAAATGGTGCAGTGATTAAATAA

Upstream 100 bases:

>100_bases
TAAGATGAAAATGAGATGACGAGATAAATGTTCGCGTCCGTTGAAATGCATAGAAATCTTAGATATTATTTGAAGTGAGA
CATTACGAGGAGGAACAGTT

Downstream 100 bases:

>100_bases
CTGTATTTTTAAAAATTAGGAGAGATAATTATGTTAATCGATACACATGTCCATTTAAATGATGAGCAATACGATGATGA
TTTGAGTGAAGTGATTACAC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDVRYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIK
QLWAKLEISNDDFIRTTEERHKHVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRTSFNWGVHVPSNPKHVVYVWIDA
LVNYISALGYLSDDESLFNRYWPADIHLMAKEIVRFHSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPN
ILIDRYGLDATRYYLMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHELDEEMEAM
ALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQKDMLGNVMAHLVENIRYAAVLLRPFLTHAPK
EIFEQLNINNPQFMEFSSLEQYGVLTESIMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDK
VEIKAATIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLMGQKSEGMILSAEKDGV
LTLVSLPSAIPNGAVIK

Sequences:

>Translated_657_residues
MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDVRYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIK
QLWAKLEISNDDFIRTTEERHKHVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRTSFNWGVHVPSNPKHVVYVWIDA
LVNYISALGYLSDDESLFNRYWPADIHLMAKEIVRFHSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPN
ILIDRYGLDATRYYLMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHELDEEMEAM
ALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQKDMLGNVMAHLVENIRYAAVLLRPFLTHAPK
EIFEQLNINNPQFMEFSSLEQYGVLTESIMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDK
VEIKAATIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLMGQKSEGMILSAEKDGV
LTLVSLPSAIPNGAVIK
>Mature_656_residues
AKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDVRYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQ
LWAKLEISNDDFIRTTEERHKHVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGHE
VELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRTSFNWGVHVPSNPKHVVYVWIDAL
VNYISALGYLSDDESLFNRYWPADIHLMAKEIVRFHSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNI
LIDRYGLDATRYYLMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHELDEEMEAMA
LETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQKDMLGNVMAHLVENIRYAAVLLRPFLTHAPKE
IFEQLNINNPQFMEFSSLEQYGVLTESIMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKV
EIKAATIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLMGQKSEGMILSAEKDGVL
TLVSLPSAIPNGAVIK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=549, Percent_Identity=32.6047358834244, Blast_Score=286, Evalue=4e-77,
Organism=Homo sapiens, GI14043022, Length=518, Percent_Identity=26.8339768339768, Blast_Score=152, Evalue=1e-36,
Organism=Escherichia coli, GI1788432, Length=678, Percent_Identity=27.8761061946903, Blast_Score=264, Evalue=2e-71,
Organism=Escherichia coli, GI1789455, Length=82, Percent_Identity=35.3658536585366, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1786861, Length=202, Percent_Identity=27.7227722772277, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212645096, Length=509, Percent_Identity=30.0589390962672, Blast_Score=248, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI71992781, Length=399, Percent_Identity=28.3208020050125, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17540794, Length=630, Percent_Identity=25.0793650793651, Blast_Score=155, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6321610, Length=523, Percent_Identity=31.7399617590822, Blast_Score=266, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6321704, Length=472, Percent_Identity=24.5762711864407, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6323414, Length=203, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21358483, Length=562, Percent_Identity=31.4946619217082, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24655311, Length=561, Percent_Identity=26.5597147950089, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 74902; Mature: 74771

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDVRYLTGTDEHGQKIQEK
CCCCEEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
AQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHKHVVEQVFERLLKQGDIYL
HHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEECHHHHHHHHHHHHHHHHHHCCCEEE
GEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGHEVELVKEESYFFNISKYTDR
ECCCCEECCCCCCEECHHHCCCCCCCCCEEECCCCCCCCCEEEEEECCCEEEEHHHHHHH
LLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRTSFNWGVHVPSNPKHVVYVWIDA
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECCCCCCEEECCCCCCEEEEEEHHH
LVNYISALGYLSDDESLFNRYWPADIHLMAKEIVRFHSIIWPILLMALDLPLPKKVFAHG
HHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
WILMKDGKMSKSKGNVVDPNILIDRYGLDATRYYLMRELPFGSDGVFTPEAFVERTNFDL
EEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCH
ANDLGNLVNRTISMVNKYFDGELPAYQGPLHELDEEMEAMALETVKSYTESMESLQFSVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSTVWKFISRTNKYIDETTPWVLAKDDSQKDMLGNVMAHLVENIRYAAVLLRPFLTHAPK
HHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
EIFEQLNINNPQFMEFSSLEQYGVLTESIMVTGQPKPIFPRLDSEAEIAYIKESMQPPAT
HHHHHCCCCCCCCEEHHHHHHHCCHHHHEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCC
EEEKEEIPSKPQIDIKDFDKVEIKAATIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAK
CHHHHHCCCCCCCCCCCCCCEEEEEEEEECHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
FYTPDDIIGKKVAVVTNLKPAKLMGQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK
HCCCHHHHCCEEEEEECCCHHHHCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDVRYLTGTDEHGQKIQEK
CCCEEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
AQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHKHVVEQVFERLLKQGDIYL
HHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEECHHHHHHHHHHHHHHHHHHCCCEEE
GEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGHEVELVKEESYFFNISKYTDR
ECCCCEECCCCCCEECHHHCCCCCCCCCEEECCCCCCCCCEEEEEECCCEEEEHHHHHHH
LLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRTSFNWGVHVPSNPKHVVYVWIDA
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECCCCCCEEECCCCCCEEEEEEHHH
LVNYISALGYLSDDESLFNRYWPADIHLMAKEIVRFHSIIWPILLMALDLPLPKKVFAHG
HHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
WILMKDGKMSKSKGNVVDPNILIDRYGLDATRYYLMRELPFGSDGVFTPEAFVERTNFDL
EEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCH
ANDLGNLVNRTISMVNKYFDGELPAYQGPLHELDEEMEAMALETVKSYTESMESLQFSVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSTVWKFISRTNKYIDETTPWVLAKDDSQKDMLGNVMAHLVENIRYAAVLLRPFLTHAPK
HHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
EIFEQLNINNPQFMEFSSLEQYGVLTESIMVTGQPKPIFPRLDSEAEIAYIKESMQPPAT
HHHHHCCCCCCCCEEHHHHHHHCCHHHHEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCC
EEEKEEIPSKPQIDIKDFDKVEIKAATIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAK
CHHHHHCCCCCCCCCCCCCCEEEEEEEEECHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
FYTPDDIIGKKVAVVTNLKPAKLMGQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK
HCCCHHHHCCEEEEEECCCHHHHCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA