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The map label for this gene is nanA

Identifier: 148266738

GI number: 148266738

Start: 353485

End: 354366

Strand: Reverse

Name: nanA

Synonym: SaurJH9_0298

Alternate gene names: 148266738

Gene position: 354366-353485 (Counterclockwise)

Preceding gene: 148266740

Following gene: 148266737

Centisome position: 12.19

GC content: 35.26

Gene sequence:

>882_bases
ATGAACAAAGATTTAAAAGGTTTATATGCAGCGTTACTCGTTCCTTTTGATGAAAATGGTCAAGTAAATGAACAAGGACT
TAAACAAATTGCTCAAAATGCCATTGAAACTGAAGAATTAGACGGTCTTTATGTAAATGGTAGCTCAGGCGAAAACTTTT
TATTAAATACAGAGCAGAAGAAGCAAGTTTTCAAGGTTGCCAAAGAGGCAGTTGGGGATAAAGTGAAATTGATTGCTCAA
GTAGGTTCGTTAGATTTAAATGAAGCCATTGAACTCGGAAAATATGCCACAGAACTCGGTTATGATGCACTTTCTGCCGT
AACACCATTCTACTATCCATTTACTTTTGAAGAAATTAGAGATTACTATTTCGATATTATCGAAGCAACACAGAACAATA
TGATTATTTATGCAATACCAGATTTAACAGGTGTGAATATTTCTATTGAACAATTCAGCGAGCTATTTAATCACGAAAAA
ATTGTTGGTGTTAAATATACAGCGCCGAACTTCTTCCTACTCGAACGTATTAGAAAAGCATTCCCAGACAAATTAATCTT
ATCTGGCTTTGATGAAATGTTAGTTCAAGCTACGATTTCTGGCGTAGATGGTGCAATTGGTTCTACATATAACGTTAATG
GTCGCCGCGCTAGAAAAATCTTCGACTTAGCACGACAAGGTCAAATTCAAGAAGCTTACCAACTACAACATGATTCAAAC
GACATTATCGAAACAGTGTTATCAATGGGGATTTATCCAACATTGAAAGAAATATTACGTCATCGTGACATTGATGCAGG
ATTACCGAAAAGACCTTTCAAACCTTTTAACGAAGCACATCGACAAACATTAGATCAACTCATTGCAAAATACGATTTAT
AG

Upstream 100 bases:

>100_bases
ATGACTACAATTTTCAAAAAGATATTGTTACATTTTATGATAGCGTTTACAATTATAGCTGTAAACTATATTATCAAATG
ATTTTTGGAGGGACTTCATA

Downstream 100 bases:

>100_bases
ACATCAAAGAATCAATACATTGTAAAGGGGATGTTGCCCATGAAAGAAGTTGGATTTGGCACACTAAACTGGGTTGCCGT
TATCATTTATCTACTAGCTA

Product: N-acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLYVNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQ
VGSLDLNEAIELGKYATELGYDALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARKIFDLARQGQIQEAYQLQHDSN
DIIETVLSMGIYPTLKEILRHRDIDAGLPKRPFKPFNEAHRQTLDQLIAKYDL

Sequences:

>Translated_293_residues
MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLYVNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQ
VGSLDLNEAIELGKYATELGYDALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARKIFDLARQGQIQEAYQLQHDSN
DIIETVLSMGIYPTLKEILRHRDIDAGLPKRPFKPFNEAHRQTLDQLIAKYDL
>Mature_293_residues
MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLYVNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQ
VGSLDLNEAIELGKYATELGYDALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARKIFDLARQGQIQEAYQLQHDSN
DIIETVLSMGIYPTLKEILRHRDIDAGLPKRPFKPFNEAHRQTLDQLIAKYDL

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily

Homologues:

Organism=Homo sapiens, GI13540533, Length=302, Percent_Identity=28.476821192053, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI31543060, Length=287, Percent_Identity=23.3449477351916, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1789620, Length=289, Percent_Identity=37.0242214532872, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082415, Length=238, Percent_Identity=26.890756302521, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786463, Length=276, Percent_Identity=23.9130434782609, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788823, Length=227, Percent_Identity=26.431718061674, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NANA_STAA1 (A7WXZ2)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001440902.1
- ProteinModelPortal:   A7WXZ2
- SMR:   A7WXZ2
- STRING:   A7WXZ2
- EnsemblBacteria:   EBSTAT00000004086
- GeneID:   5560269
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0312
- eggNOG:   COG0329
- GeneTree:   EBGT00050000024245
- HOGENOM:   HBG358848
- OMA:   NTIETEE
- ProtClustDB:   PRK04147
- BioCyc:   SAUR418127:SAHV_0312-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01237
- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR005264
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146

Pfam domain/function: PF00701 DHDPS

EC number: =4.1.3.3

Molecular weight: Translated: 33101; Mature: 33101

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLYVNGSSGENFLLNTEQK
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCHHH
KQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYDALSAVTPFYYPFTFEEIR
HHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEKIVGVKYTAPNFFLLERIRKA
HHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
FPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARKIFDLARQGQIQEAYQLQHDSN
CCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCH
DIIETVLSMGIYPTLKEILRHRDIDAGLPKRPFKPFNEAHRQTLDQLIAKYDL
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLYVNGSSGENFLLNTEQK
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCHHH
KQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYDALSAVTPFYYPFTFEEIR
HHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEKIVGVKYTAPNFFLLERIRKA
HHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
FPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARKIFDLARQGQIQEAYQLQHDSN
CCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCH
DIIETVLSMGIYPTLKEILRHRDIDAGLPKRPFKPFNEAHRQTLDQLIAKYDL
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA