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The map label for this gene is norB [H]

Identifier: 148266698

GI number: 148266698

Start: 310997

End: 312373

Strand: Reverse

Name: norB [H]

Synonym: SaurJH9_0258

Alternate gene names: 148266698

Gene position: 312373-310997 (Counterclockwise)

Preceding gene: 148266701

Following gene: 148266696

Centisome position: 10.75

GC content: 37.69

Gene sequence:

>1377_bases
GTGAATAACACACAATCTTCACCACGCAGTAATATTATTATTGCGATTATGTTGTCTGCATTAACATATTGGTTGTTTGC
ACAATCATTTATTAATATAGGACCTCTCGTTGGTCAAACGTATCAAACCTCTCCTGCCGTGTTAAATTTATCTATTAGTT
TAACTTCCTTCGCCACAGGTATCTTCATGGTGGCTGCAGGTGATATTGCTGATAAAATAGGACAACTGAGAATGACATAC
ATGGGTCTCATAATCAGTATGTTTGCATCTCTTCTATTAATTATATCGGACATCACTGCACTGCTCATCATCGGTAGAAT
TTTACAAGGTCTATCAGCAGCTATCTTGTTACCTTCAACAGTTGGCGTTTTAAATAATCAATTTAAAGGAGAACATTTAA
GACGAGCGATTAGTTATCTAATGATTAGTACTGTTGGGGGCATCGGCCTAGCTGGTGTTATCGGCGGTTTAATTGCTACA
AATTTCGGATGGCAAATGAATTTCATCATTAGTATAGTCATTGCTTTCATTGCCATATTACTTCTAAAAGGCACACCTGA
AAAAGTAAGTCAACATAGCCACCGTCATCCGTTCGATTACAAAGGTATGTCGATTTTCGCTGTTATGATTGGTAGCTTTA
CATTATTGTTAACACAAGGATTCGAACAAGGTTGGTTTAGTACATTCTCATTCATTTGTCTGAGCATTTTTATCATCACT
ACGCTGATATTCATCATCATCGAACGTCGACATGAAGTACCTTTTATTGATTTCTCAGTATTACGCAACCGTCCGTTCAT
TGGTGCATTTTTAAATAACTTTGTTTTAAATAGCGGTCTAGGCGTTACAGTGGTCTTTTTCATATATGCTCAAACACACC
TTGGTTTATCAGCTGCGCAATCTGGACTTGTTACATTGCCATATGCCATTGTGGCAGTTGCGATGATTCGTTTAGGTGAA
AAAGCAACATTACGTTTCGGTGGCAAATTGATGCTCATCATTGGTCCGTTGTTCCCTGTCATCGGCATCACTATTATTAG
CATGACTCAATTATCGGCATCACAATATGTCATTGCAGTTATCATTGGTTTCGTCATTTGCGCAATTGGAAATGGTTTAG
TCGCAACACCCGGCCTTACAATTGCAATTTTCAGTATGCCAAATGAAAAAGTTGGTTTAGCTACAGGATTATATAAAATG
AGTGGTACATTAGGTGGCGCCTTTGGTATAGCACTAAGTACTACAGTTTTCAGTATGTTACAACTAAACTATGCACCAAG
TGTAGCTGCAACCGTAACATTTATCGTCAGCATTGTATTGATGATCCTTGGCTCATTGTCTGCATACATGATCATTCCAA
AAACAGTTAAATCTTAA

Upstream 100 bases:

>100_bases
TAAGGAAATCATGACTTATGTGTGAGTGAACGATCATCTATACATCCGTTCACTTCATCTCATGACTTTCTATATTTAAT
TTTTACAAGGAGTGACATCT

Downstream 100 bases:

>100_bases
ATATAATAGAAGAATTATGTTTCGAAATATCTTTATCACTTTAAAATGATATACAAGAAATTCAAGAAAAATAAGCGAAG
AACGTTTTTCTCTGTTCTTC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTY
MGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
NFGWQMNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSFICLSIFIIT
TLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGE
KATLRFGGKLMLIIGPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKM
SGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS

Sequences:

>Translated_458_residues
MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTY
MGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
NFGWQMNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSFICLSIFIIT
TLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGE
KATLRFGGKLMLIIGPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKM
SGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS
>Mature_458_residues
MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTY
MGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
NFGWQMNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSFICLSIFIIT
TLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGE
KATLRFGGKLMLIIGPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKM
SGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS

Specific function: Multidrug efflux pump that acts independently of norA and is one of the factors that confers resistance against diverse quinolones and chemical compounds [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=374, Percent_Identity=24.331550802139, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI87081983, Length=354, Percent_Identity=26.8361581920904, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788710, Length=407, Percent_Identity=23.5872235872236, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790195, Length=303, Percent_Identity=23.1023102310231, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49094; Mature: 49094

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHH
IFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPST
HHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIATNFGWQMNFIISIVIAFIAIL
HHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSFICLSIFIIT
HHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
TLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQ
HHHHHHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHC
SGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLIIGPLFPVIGITIISMTQLSASQYVIAV
CCCCHHHHHHHHHHHHHCCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHHCHHHHHHHH
IIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKMSGTLGGAFGIALSTTVFSML
HHHHHHHHHCCCEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS
HHCCCCHHHHHHHHHHHHHHHHHHCCHHEEEECHHHCC
>Mature Secondary Structure
MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHH
IFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPST
HHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIATNFGWQMNFIISIVIAFIAIL
HHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSFICLSIFIIT
HHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
TLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQ
HHHHHHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHC
SGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLIIGPLFPVIGITIISMTQLSASQYVIAV
CCCCHHHHHHHHHHHHHCCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHHCHHHHHHHH
IIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKMSGTLGGAFGIALSTTVFSML
HHHHHHHHHCCCEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS
HHCCCCHHHHHHHHHHHHHHHHHHCCHHEEEECHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA