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The map label for this gene is ldh1

Identifier: 148266665

GI number: 148266665

Start: 275113

End: 276066

Strand: Direct

Name: ldh1

Synonym: SaurJH9_0225

Alternate gene names: 148266665

Gene position: 275113-276066 (Clockwise)

Preceding gene: 148266660

Following gene: 148266668

Centisome position: 9.46

GC content: 37.21

Gene sequence:

>954_bases
ATGAACAAATTTAAAGGGAACAAAGTTGTATTAATAGGTAATGGTGCAGTAGGTTCAAGCTACGCATTTTCATTAGTGAA
CCAAAGCATTGTTGATGAATTAGTCATCATTGATTTAGACACTGAAAAAGTTCGAGGAGATGTTATGGATTTAAAACATG
CCACACCATATTCTCCAACAACAGTTCGTGTGAAAGCTGGCGAATACAGTGATTGTCATGATGCGGATCTAGTTGTCATC
TGTGCTGGTGCTGCACAAAAACCTGGAGAAACACGTTTAGATTTAGTATCTAAAAACTTGAAAATATTCAAATCAATTGT
TGGTGAAGTAATGGCATCAAAATTTGATGGTATTTTCTTGGTAGCTACAAATCCTGTTGATATTTTAGCGTATGCAACAT
GGAAATTCTCTGGTTTACCTAAAGAACGTGTTATAGGTTCTGGTACAATTTTAGACTCTGCACGCTTTAGATTATTGTTA
AGCGAAGCGTTCGATGTTGCGCCACGTAGCGTCGATGCTCAAATTATTGGTGAACATGGTGACACTGAATTACCAGTATG
GTCACACGCTAATATTGCGGGTCAACCTTTGAAGACATTACTTGAACAACGTCCTGAGGGCAAAGCGCAAATTGAACAAA
TTTTTGTTCAAACACGTGATGCAGCATATGACATTATTCAAGCTAAAGGTGCCACTTATTATGGTGTTGCAATGGGATTA
GCTAGAATTACTGAAGCGATTTTCAGAAATGAAGATGCCGTATTGACTGTATCAGCATTATTAGAAGGCGAATATGATGA
AGAAGATGTTTATATTGGTGTTCCAGCAGTCATCAATAGAAACGGTATTCGCAACGTCGTAGAAATCCCATTAAACGACG
AAGAACAAAGCAAGTTCGCACATTCAGCTAAAACATTAAAAGATATTATGGCTGAAGCAGAAGAACTTAAATAA

Upstream 100 bases:

>100_bases
GAGATGTTTATTTGCTTTTGTATCGTCAATAAGCAGCATTAAACTAACATATTTGAAGCTACACGTATGTTAGTGAATTA
ATCATAAGGGAGTTTTTGTA

Downstream 100 bases:

>100_bases
CTTTTTATAAAATCTATACCATTCCAAAAATTGTAAAACCTTACCCAAAAAATTGTATAAAGGGCTATTTGATACAACTA
TATGTGTCGAGTAGCCCTAT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 1

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVI
CAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGL
ARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK

Sequences:

>Translated_317_residues
MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVI
CAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGL
ARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK
>Mature_317_residues
MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVI
CAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGL
ARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK

Specific function: Appears to be the primary factor that allows S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI47059044, Length=312, Percent_Identity=40.3846153846154, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI221136809, Length=312, Percent_Identity=40.3846153846154, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI5031857, Length=311, Percent_Identity=39.871382636656, Blast_Score=247, Evalue=8e-66,
Organism=Homo sapiens, GI260099723, Length=311, Percent_Identity=39.871382636656, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI15082234, Length=309, Percent_Identity=39.1585760517799, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=40.9836065573771, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=40.9836065573771, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI291575128, Length=316, Percent_Identity=37.3417721518987, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI4557032, Length=316, Percent_Identity=37.3417721518987, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI260099727, Length=194, Percent_Identity=45.8762886597938, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI260099725, Length=194, Percent_Identity=45.8762886597938, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI207028494, Length=214, Percent_Identity=39.7196261682243, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI103472011, Length=308, Percent_Identity=27.5974025974026, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI103472015, Length=182, Percent_Identity=29.6703296703297, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1789632, Length=327, Percent_Identity=26.9113149847095, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17535107, Length=292, Percent_Identity=39.3835616438356, Blast_Score=229, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17554310, Length=301, Percent_Identity=26.578073089701, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17136226, Length=309, Percent_Identity=39.1585760517799, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI45550422, Length=326, Percent_Identity=29.7546012269939, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24647881, Length=316, Percent_Identity=28.7974683544304, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24663599, Length=326, Percent_Identity=23.3128834355828, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH1_STAA1 (A7WXP7)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001440830.1
- ProteinModelPortal:   A7WXP7
- SMR:   A7WXP7
- STRING:   A7WXP7
- EnsemblBacteria:   EBSTAT00000003583
- GeneID:   5561348
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0240
- eggNOG:   COG0039
- GeneTree:   EBGT00050000024431
- HOGENOM:   HBG566126
- OMA:   MDLNHGV
- ProtClustDB:   CLSK884508
- BioCyc:   SAUR418127:SAHV_0240-MONOMER
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 34570; Mature: 34570

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 179-179 BINDING 92-92 BINDING 124-124 BINDING 155-155 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPT
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHCHHHHCCCCCCCCCE
TVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFL
EEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLLSEAFDVAPRSVDAQIIGEHG
EECCCEEEEEEEEEEECCCCHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCEEECCCC
DTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGL
CCCCCEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
ARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFA
HHHHHHHHCCCCCEEEEEHHHCCCCCCCCEEECCCCEECCCCCCCEEECCCCCCHHHHHH
HSAKTLKDIMAEAEELK
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPT
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHCHHHHCCCCCCCCCE
TVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFL
EEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLLSEAFDVAPRSVDAQIIGEHG
EECCCEEEEEEEEEEECCCCHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCEEECCCC
DTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGL
CCCCCEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
ARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFA
HHHHHHHHCCCCCEEEEEHHHCCCCCCCCEEECCCCEECCCCCCCEEECCCCCCHHHHHH
HSAKTLKDIMAEAEELK
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA