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The map label for this gene is gsiB [H]

Identifier: 148266661

GI number: 148266661

Start: 271010

End: 272422

Strand: Reverse

Name: gsiB [H]

Synonym: SaurJH9_0221

Alternate gene names: 148266661

Gene position: 272422-271010 (Counterclockwise)

Preceding gene: 148266662

Following gene: 148266659

Centisome position: 9.37

GC content: 31.07

Gene sequence:

>1413_bases
ATGCATTCATCTGGCAAAGACTTAAATATTTCGTTACCGTTAAAAACAAAGTCTATTGCACCTTATGAAACAGATGTACC
AGTTAAGATTGGTGCTGCAGAATCATTATTTAAAACAAATGATCAAGGAAAAATAGAAAAAGCATTAGTGAAATCGTATC
ATCAACCAAATGATACAACGTTAGATATAGAATTAAAAGATAATATTAAATTTCAAAACGGTCAAAAATTGACTGCAGAA
AAAGTGAAATCTAGCCTTGAAAATAGCATGAAAAAAAGCGACTTGGTCAAATATTCATTACCAATATCATCAATTACCGC
TAAAGGTCAAAAACTGACAATTAAAACCAACTCCGCTTACCCTGAACTTGTATCTGAATTAGCTAATCCTTTTATGGCAA
TTTATTATACAGATGCTAAATCAGATGTTAATCAAACTCCTGTTGGTACAGGCCCTTACCAAATAAAAGATTATAAGCAA
TCTCGAAAAATATCATTGTCGAATTTTAAGGACTATTGGCAAGGTAAACCGAAACTTGATCATATTACTGTGACGTACCA
AGAAGACGGCAATAATCGCGTCAGAAATTTAGAATCTCAAAAAGATGATTTAATAACTGATGTCCCAGTTAATAAAGTTC
ATGACATAGAAAATAATCAAAATTTAAAAGTGTCAAAAGAATCTGGATTTAGAACTTCTTTACTTATGTATAATCATACT
AATAAAAAAATGACTAAATCCGTTCGTGAAGCATTAGATCATATCATTGATAGACAAGGTATTGCAGATCATATTTATCA
AGGTTATGCGAAACCTGCAACGAGTCCATTTAATGATAAAATTCCATATATTAAAGAACCTAAGTTAACCAAACAAAATA
TCGAACAAGCAAAAACGTTATTAGCTAAAGATGGTTATACAAAAGAACACCCTTTAAAAATTAAGTTAATTACGTATGAC
GGTCGTCCAGAGCTATCGAAAATTGCGCAAGTATTACAATCAGATGCTAAAAAAGCAAATATTGAAATCGACATTAAAAG
TGTTGATGACATAGAAGGTTACTTAAAAGACCGTTCTGCATGGGATGCAACGATGTATAGTTTCGGAACAATCCCTCGCG
GTGATACAGGTTATTTCTTTAATCAAGCATATAAAAAAGATGGTGCTATTAATAAAGGAGACTACAATAATAGCAACGTC
GATGATTTAATTAATCAATTGAATCATACTGTAGATGTTAAGGAACGTCACAATATTTCTAATGATATTATTAAATTATC
AAGTCGAGATGTACCAAACAGTTATATTGCTTACAACGATCAAATAGTCGCTGCTAACTCTAAGGTTAAAAATTATAAAG
TAACACCTGAAGGTATCTATTTAATCGATTATCGAACAACGATTGAACGATAA

Upstream 100 bases:

>100_bases
GGTTTTACATAGTAATTAGAAAGAGAGTGTTAATGAAATGAAGTTTAAAAGACTAGCAACTATATTTTCAGCAGTATTAG
TGTTATCAGGATGTGGCTCA

Downstream 100 bases:

>100_bases
GGGAGTGTGGGACATTGATTTATGTCTCAGCCCTGCTTCAACTATAAAAACCACGCCGAAGTCATCCAATATGATTATGG
CGTGGTTTAATTTGCTTATA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAE
KVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYYTDAKSDVNQTPVGTGPYQIKDYKQ
SRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVHDIENNQNLKVSKESGFRTSLLMYNHT
NKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLKIKLITYD
GRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNV
DDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER

Sequences:

>Translated_470_residues
MHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAE
KVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYYTDAKSDVNQTPVGTGPYQIKDYKQ
SRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVHDIENNQNLKVSKESGFRTSLLMYNHT
NKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLKIKLITYD
GRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNV
DDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER
>Mature_470_residues
MHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAE
KVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYYTDAKSDVNQTPVGTGPYQIKDYKQ
SRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVHDIENNQNLKVSKESGFRTSLLMYNHT
NKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLKIKLITYD
GRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNV
DDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=488, Percent_Identity=25.6147540983607, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789887, Length=463, Percent_Identity=23.3261339092873, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787762, Length=440, Percent_Identity=21.8181818181818, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789966, Length=361, Percent_Identity=22.4376731301939, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787551, Length=204, Percent_Identity=25.9803921568627, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 53248; Mature: 53248

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTT
CCCCCCCEEEEECCCCCCCCCCCCCCCEEECCHHHHHHCCCCCHHHHHHHHHCCCCCCCE
LDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAY
EEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCCEEEEEECCCC
PELVSELANPFMAIYYTDAKSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLD
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEECCHHHCCCEEHHHHHHHHCCCCCCE
HITVTYQEDGNNRVRNLESQKDDLITDVPVNKVHDIENNQNLKVSKESGFRTSLLMYNHT
EEEEEEEECCCCHHHCCCCHHCCCEECCCCCHHCCCCCCCCEEEECCCCCEEEEEEEECC
NKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTL
CHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSA
HHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHCEEEEEECCCHHHHHHHHCCCH
WDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNIS
HHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCEECCHHHCCCC
NDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER
HHEEEECCCCCCCCEEEECCEEEEECCCCCEEEECCCCEEEEEEHHCCCC
>Mature Secondary Structure
MHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTT
CCCCCCCEEEEECCCCCCCCCCCCCCCEEECCHHHHHHCCCCCHHHHHHHHHCCCCCCCE
LDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAY
EEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCCEEEEEECCCC
PELVSELANPFMAIYYTDAKSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLD
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEECCHHHCCCEEHHHHHHHHCCCCCCE
HITVTYQEDGNNRVRNLESQKDDLITDVPVNKVHDIENNQNLKVSKESGFRTSLLMYNHT
EEEEEEEECCCCHHHCCCCHHCCCEECCCCCHHCCCCCCCCEEEECCCCCEEEEEEEECC
NKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTL
CHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSA
HHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHCEEEEEECCCHHHHHHHHCCCH
WDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNIS
HHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCEECCHHHCCCC
NDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER
HHEEEECCCCCCCCEEEECCEEEEECCCCCEEEECCCCEEEEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA