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The map label for this gene is pflA

Identifier: 148266651

GI number: 148266651

Start: 254920

End: 255675

Strand: Direct

Name: pflA

Synonym: SaurJH9_0211

Alternate gene names: 148266651

Gene position: 254920-255675 (Clockwise)

Preceding gene: 148266650

Following gene: 148266652

Centisome position: 8.77

GC content: 36.38

Gene sequence:

>756_bases
ATGCTTAAGGGACACTTACATTCTGTCGAAAGTTTAGGTACTGTCGATGGACCGGGATTAAGATATATATTATTTACACA
AGGATGCTTACTTAGATGCTTGTATTGCCACAATCCAGATACTTGGAAAATTAGTGAGCCATCAAGAGAAGTCACAGTTG
ATGAAATGGTGAATGAAATATTACCATACAAACCATACTTTGATGCATCGGGTGGCGGTGTAACAGTCAGTGGTGGCGAA
CCATTGTTACAAATGCCATTCTTAGAAAAATTATTTGCGGAATTAAAAGAAAATGGTGTGCACACTTGCTTAGACACATC
GGCTGGATGTGCTAATGATACAAAAGCATTTCAAAGGCATTTTGAAGAATTACAAAAACATACAGACTTGATATTATTAG
ATATAAAACATATTGATAATGACAAACATATTAGATTGACAGGAAAGCCTAATACACACATCCTTAACTTCGCGCGCAAA
CTGTCAGATATGAAACAACCTGTATGGATTCGACATGTCCTTGTGCCTGGTTATTCTGATGATAAAGACGATTTAATTAA
ACTAGGGGAATTCATTAATTCTCTTGATAACGTCGAAAAGTTTGAAATTCTGCCATATCATCAGTTAGGTGTTCATAAGT
GGAAAACATTGGGCATTGCATATGAATTAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTAT
GTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAA

Upstream 100 bases:

>100_bases
TACGCTGTTAACTTCATTAAATTAACACGTGAACAACAATTAGATGTAATTTCTCGTACATTCCATGAAAGTATGTAACA
AAATTTAAGGTGGGAGCACT

Downstream 100 bases:

>100_bases
ATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAG
TATTAGCAAGACGTAGTAAG

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKISEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGE
PLLQMPFLEKLFAELKENGVHTCLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLGIAYELEDVEAPDDEAVKAAYRY
VNFKGKIPVEL

Sequences:

>Translated_251_residues
MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKISEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGE
PLLQMPFLEKLFAELKENGVHTCLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLGIAYELEDVEAPDDEAVKAAYRY
VNFKGKIPVEL
>Mature_251_residues
MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKISEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGE
PLLQMPFLEKLFAELKENGVHTCLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLGIAYELEDVEAPDDEAVKAAYRY
VNFKGKIPVEL

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family

Homologues:

Organism=Escherichia coli, GI1787130, Length=232, Percent_Identity=47.4137931034483, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1790389, Length=271, Percent_Identity=25.4612546125461, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790839, Length=271, Percent_Identity=27.3062730627306, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI226510931, Length=194, Percent_Identity=27.8350515463918, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PFLA_STAA3 (Q2FK43)

Other databases:

- EMBL:   CP000255
- RefSeq:   YP_492935.1
- ProteinModelPortal:   Q2FK43
- SMR:   Q2FK43
- STRING:   Q2FK43
- EnsemblBacteria:   EBSTAT00000037291
- GeneID:   3912962
- GenomeReviews:   CP000255_GR
- KEGG:   saa:SAUSA300_0221
- eggNOG:   COG1180
- GeneTree:   EBGT00050000024170
- HOGENOM:   HBG554871
- OMA:   ETCGTVD
- ProtClustDB:   CLSK884495
- GO:   GO:0005737
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197
- TIGRFAMs:   TIGR02493

Pfam domain/function: PF04055 Radical_SAM

EC number: =1.97.1.4

Molecular weight: Translated: 28499; Mature: 28499

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKISEPSREVTVDEMVNEI
CCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHEEHHCCCCCCEEECCCCCCEEHHHHHHHH
LPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHTCLDTSAGCANDTKAFQRH
CCCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
FEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARKLSDMKQPVWIRHVLVPGYSD
HHHHHHCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
DKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLGIAYELEDVEAPDDEAVKAAYRY
CHHHHHHHHHHHHHHCCCCCEEECCHHHHCCHHHHEEEEEEEECCCCCCCHHHHHHHHHH
VNFKGKIPVEL
HCCCCCCCCCC
>Mature Secondary Structure
MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKISEPSREVTVDEMVNEI
CCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHEEHHCCCCCCEEECCCCCCEEHHHHHHHH
LPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHTCLDTSAGCANDTKAFQRH
CCCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
FEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARKLSDMKQPVWIRHVLVPGYSD
HHHHHHCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
DKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLGIAYELEDVEAPDDEAVKAAYRY
CHHHHHHHHHHHHHHCCCCCEEECCHHHHCCHHHHEEEEEEEECCCCCCCHHHHHHHHHH
VNFKGKIPVEL
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA