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The map label for this gene is cycB [H]

Identifier: 148266639

GI number: 148266639

Start: 239341

End: 240612

Strand: Direct

Name: cycB [H]

Synonym: SaurJH9_0199

Alternate gene names: 148266639

Gene position: 239341-240612 (Clockwise)

Preceding gene: 148266638

Following gene: 148266640

Centisome position: 8.23

GC content: 34.2

Gene sequence:

>1272_bases
ATGTCTAAAATTTTAAAATGTATCACGTTAGCCGTGGTAATGTTATTAATCGTAACTGCATGTGGCCCTAATCGTTCGAA
AGAAGATATTGATAAAGCATTGAATAAAGATAATTCTAAAGACAAGCCTAACCAACTTACGATGTGGGTGGATGGCGACA
AGCAAATGGCGTTTTATAAAAAAATTACGGATCAATATACTAAAAAAACTGGCATCAAAGTAAAGCTTGTAAATATTGGT
CAAAATGATCAACTAGAAAATATTTCGCTAGACGCTCCTGCAGGAAAAGGTCCAGATATCTTTTTCTTAGCACATGATAA
TACTGGAAGTGCCTATCTACAAGGCTTAGCTGCTGAAATCAAATTATCAAAAGATGAGTTGAAAGGTTTCAATAAGCAAG
CACTTAAAGCGATGAATTATGACAATAAGCAACTAGCATTGCCAGCTATCGTAGAAACAACCGCACTTTTCTATAATAAA
AAATTAGTGAAAAATGCACCGCAAACGTTAGAAGAAGTTGAAGCTAATGCTGCCAAGCTAACTGATAGTAAAAAGAAACA
ATACGGTATGTTATTTGATGCTAAAAATTTCTATTTTAATTATCCGTTTTTATTCGGCAATGATGATTATATTTTCAAGA
AAAATGGCAGTGAATATGATATTCATCAGCTAGGACTAAATTCAAAACATGTCGTCAAAAATGCTGAACGATTACAAAAA
TGGTACGACAAAGGGTATCTTCCTAAGGCAGCAACACATGATGTCATGATTGGTCTTTTTAAAGAAGGAAAAGTAGGACA
ATTTGTCACTGGACCGTGGAACATTAATGAATATCAAGAAACGTTTGGTAAAGATTTAGGAGTAACAACATTACCTACAG
ATGGTGGCAAACCTATGAAACCATTTCTCGGTGTACGTGGTTGGTATTTATCTGAATATAGTAAACATAAGTATTGGGCT
AAAGATTTAATGCTGTATATCACTAGTAAAGATACATTACAAAAATATACAGATGAAATGAGCGAAATTACTGGACGTGT
TGACGTGAAATCATCTAATCCAAATTTAAAAGTGTTTGAAAAGCAAGCACGTCATGCTGAACCGATGCCTAATATTCCTG
AAATGCGACAAGTTTGGGAACCGATGGGCAATGCAAGCATATTTATCTCAAATGGTAAGAATCCTAAACAAGCGTTAGAT
GAGGCGACGAATGATATAACGCAAAATATTAAGATTCTTCATCCATCACAAAATGATAAGAAAGGAGATTAG

Upstream 100 bases:

>100_bases
AGTGATGACGAACGACAAGATTACACTAGCATTTGATATGAATAAGTGTCACTTTTTTGATGAAAAAACAGGAAATCGTA
TCGTCTAAGGGGGAGTATTC

Downstream 100 bases:

>100_bases
TTATGACGAAACGTAACCCTAAATTAGCGGCATTATTATCTGTTATACCTGGTTTGGGACAGTTTTATAATAAAAGACCC
ATTAAAGGTACGATATTTTT

Product: extracellular solute-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPNQLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIG
QNDQLENISLDAPAGKGPDIFFLAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSEYDIHQLGLNSKHVVKNAERLQK
WYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNINEYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWA
KDLMLYITSKDTLQKYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNGKNPKQALD
EATNDITQNIKILHPSQNDKKGD

Sequences:

>Translated_423_residues
MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPNQLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIG
QNDQLENISLDAPAGKGPDIFFLAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSEYDIHQLGLNSKHVVKNAERLQK
WYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNINEYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWA
KDLMLYITSKDTLQKYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNGKNPKQALD
EATNDITQNIKILHPSQNDKKGD
>Mature_422_residues
SKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPNQLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQ
NDQLENISLDAPAGKGPDIFFLAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNKK
LVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSEYDIHQLGLNSKHVVKNAERLQKW
YDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNINEYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAK
DLMLYITSKDTLQKYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNGKNPKQALDE
ATNDITQNIKILHPSQNDKKGD

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=383, Percent_Identity=30.0261096605744, Blast_Score=151, Evalue=7e-38,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 47851; Mature: 47720

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPNQLTMWVDGDKQMAFYK
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHH
KITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFFLAHDNTGSAYLQGLAAEI
HHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHE
KLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNKKLVKNAPQTLEEVEANAAKL
EECHHHHCCCCHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSEYDIHQLGLNSKHVVKNAERLQK
CCHHHHHHCEEEECCCCEEECCEEECCCCEEECCCCCCCCHHHCCCCCHHHHHHHHHHHH
WYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNINEYQETFGKDLGVTTLPTDGGKPMK
HHHCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCCHHHHHHCCCCCCEECCCCCCCCCC
PFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQKYTDEMSEITGRVDVKSSNPNLKVFE
HHHCCCCHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHCCEEEEECCCCCCEEEE
KQARHAEPMPNIPEMRQVWEPMGNASIFISNGKNPKQALDEATNDITQNIKILHPSQNDK
HHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEECCCCCCC
KGD
CCC
>Mature Secondary Structure 
SKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPNQLTMWVDGDKQMAFYK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHH
KITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFFLAHDNTGSAYLQGLAAEI
HHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHE
KLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNKKLVKNAPQTLEEVEANAAKL
EECHHHHCCCCHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSEYDIHQLGLNSKHVVKNAERLQK
CCHHHHHHCEEEECCCCEEECCEEECCCCEEECCCCCCCCHHHCCCCCHHHHHHHHHHHH
WYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNINEYQETFGKDLGVTTLPTDGGKPMK
HHHCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCCHHHHHHCCCCCCEECCCCCCCCCC
PFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQKYTDEMSEITGRVDVKSSNPNLKVFE
HHHCCCCHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHCCEEEEECCCCCCEEEE
KQARHAEPMPNIPEMRQVWEPMGNASIFISNGKNPKQALDEATNDITQNIKILHPSQNDK
HHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEECCCCCCC
KGD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]