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The map label for this gene is rfbX [H]

Identifier: 148266558

GI number: 148266558

Start: 140198

End: 141628

Strand: Direct

Name: rfbX [H]

Synonym: SaurJH9_0118

Alternate gene names: 148266558

Gene position: 140198-141628 (Clockwise)

Preceding gene: 148266557

Following gene: 148266559

Centisome position: 4.82

GC content: 31.45

Gene sequence:

>1431_bases
ATGAAGAGTGATTCACTAAAAGAAAATATTATTTATCAAGGGCTATACCAATTGATTAGAACGATGACACCACTGATTAC
AATACCCATTATTTCACGTGCATTTGGTCCCAGTGGTGTGGGTATTGTTTCATTTTCTTTCAATATCGTGCAATACTTTT
TGATGATTGCAAGTGTTGGCGTTCAGTTATATTTTAATAGAGTTATCGCGAAGTCTGTTAACGACAAACGGCAATTGTCT
CAGCAGTTTTGGGATATCTTTGTCAGTAAATTATTTTTAGCGTTAACAGTTTTTGCGATGTATATGGTCGTAATTACTAT
ATTTATTGATGATTACTATCTTATTTTCCTACTACAAGGAATCTATATTATAGGTGCAGCACTCGATATTTCATGGTTTT
ATGCTGGAACTGAAAAGTTTAAAATTCCTAGCCTCAGTAATATTGTTGCGTCTGGTATTGTATTAAGTGTAGTTGTTATT
TTTGTCAAAGATCAATCAGATTTATCATTGTATGTATTTACTATTGCTATTGTGACGGTATTAAACCAATTACCTTTGTT
TATCTATTTAAAACGATACATTAGCTTTGTTTCGGTTAATTGGATACACGTCTGGCAATTGTTTCGTTCGTCATTAGCAT
ACTTATTACCAAATGGACAGCTCAACTTATATACTAGTATTTCTTGCGTTGTTCTTGGTTTAGTAGGCACATACCAACAA
GTTGGTATCTTTTCTAACGCATTTAATATTTTAACGGTCGCAATCATAATGATTAATACATTTGATCTTGTAATGATTCC
GCGTATTACCAAAATGTCTATCCAGCAATCACATAGTTTAACTAAAACGTTAGCTAATAATATGAATATTCAATTGATAT
TAACAATACCTATGGTCTTTGGTTTAATTGCAATTATGCCATCATTTTATTTATGGTTCTTTGGTGAGGAATTCGCATCA
ACTGTCCCATTGATGACCATTTTAGCGATACTTGTATTAATCATTCCTTTAAATATGTTGATAAGCAGGCAATATTTATT
AATAGTGAATAAAATAAGATTATATAATGCGTCAATTACTATTGGTGCGGTAATGAATTTAGTATTATGTCTTGTTTTGA
TATATTTTTATGGAATTTACGGTGCGGCTATTGCACGTTTAATTACAGAGTTTATCTTACTTATTTGGCGATTTGTTGAT
ATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCCGTTATGATGTTTATTGTGCT
TGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTT
TATTAATGATGACTATGAAAAATCAATACGTATGGCAAATATTGAGGCATCTTCGACATAAAACAATTTAA

Upstream 100 bases:

>100_bases
TACTTTTTAACAGTATCATTTAATAACTCAAGATATGTCGCTTTTATTTTAGGAATTATCGTCTTTATTGTTCAATATGA
AAAGATGGAAAGGGATCGTA

Downstream 100 bases:

>100_bases
GTATCGGTAACGCTATACTTTAGAAATTTAAGATTAAGAAGAAAAGGCAATTTCTTATTGAAAAATGGAAGTTGTCTTTT
TTAATTCTCTTTAAAAGCGG

Product: polysaccharide biosynthesis protein

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLS
QQFWDIFVSKLFLALTVFAMYMVVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQ
VGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQSHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFAS
TVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFILLIWRFVD
ITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI

Sequences:

>Translated_476_residues
MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLS
QQFWDIFVSKLFLALTVFAMYMVVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQ
VGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQSHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFAS
TVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFILLIWRFVD
ITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI
>Mature_476_residues
MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLS
QQFWDIFVSKLFLALTVFAMYMVVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQ
VGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQSHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFAS
TVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFILLIWRFVD
ITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=273, Percent_Identity=23.4432234432234, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 54118; Mature: 54118

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
VQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYMVVITIFIDDYYLIFLLQG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVIFVKDQSDLSLYVFTIAIVTV
HHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
LNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
VGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQSHSLTKTLANNMNIQLILTIPMVF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
GLIAIMPSFYLWFFGEEFASTVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASIT
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCH
IGAVMNLVLCLVLIYFYGIYGAAIARLITEFILLIWRFVDITKINVKLNIVSTIQCVIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEHHHHHHHHHHH
VMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
VQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYMVVITIFIDDYYLIFLLQG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVIFVKDQSDLSLYVFTIAIVTV
HHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
LNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
VGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQSHSLTKTLANNMNIQLILTIPMVF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
GLIAIMPSFYLWFFGEEFASTVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASIT
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCH
IGAVMNLVLCLVLIYFYGIYGAAIARLITEFILLIWRFVDITKINVKLNIVSTIQCVIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEHHHHHHHHHHH
VMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]