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The map label for this gene is yfiY [H]

Identifier: 148266542

GI number: 148266542

Start: 121392

End: 122384

Strand: Reverse

Name: yfiY [H]

Synonym: SaurJH9_0102

Alternate gene names: 148266542

Gene position: 122384-121392 (Counterclockwise)

Preceding gene: 148266561

Following gene: 148266541

Centisome position: 4.21

GC content: 32.23

Gene sequence:

>993_bases
ATGAATAAAGTAATTAAAATGCTTGTTGTTACGCTTGCTTTCCTACTTGTTTTAGCAGGATGTAGTGGGAATTCAAATAA
ACAATCATCTGATAACAAAGATAAGGAAACAACTTCAATTAAACATGCAATGGGTACAACTGAAATTAAAGGGAAACCAA
AGCGTGTTGTTACGCTATATCAAGGTGCCACTGACGTCGCTGTATCTTTAGGTGTTAAACCTGTAGGTGCTGTAGAATCA
TGGACACAAAAACCGAAATTCGAATACATAAAAAATGATTTAAAAGATACTAAGATTGTAGGTCAAGAACCTGCACCTAA
CTTAGAGGAAATCTCTAAATTAAAACCGGACTTAATTGTCGCGTCAAAAGTTAGAAATGAAAAAGTTTACGATCAATTAT
CTAAAATCGCACCAACAGTTTCTACTGATACAGTTTTCAAATTCAAAGATACAACTAAGTTAATGGGGAAAGCTTTAGGG
AAAGAAAAAGAAGCTGAAGATTTACTTAAAAAGTACGATGATAAAGTAGCTGCATTCCAAAAAGATGCAAAAGCAAAGTA
TAAAGATGCATGGCCATTGAAAGCTTCAGTTGTTAACTTCCGTGCTGATCATACAAGAATTTATGCTGGTGGATATGCTG
GTGAAATCTTAAATGATTTAGGATTCAAACGTAATAAAGACTTACAAAAACAAGTTGATAATGGTAAAGATATTATCCAA
CTTACATCTAAAGAAAGCATTCCATTAATGAACGCTGATCATATTTTTGTAGTAAAATCAGATCCAAATGCGAAAGATGC
TGCATTAGTTAAAAAGACTGAAAGCGAATGGACTTCAAGTAAAGAGTGGAAAAATTTAGACGCAGTTAAAAACAACCAAG
TATCTGATGATTTAGATGAAATCACTTGGAACTTAGCTGGCGGATATAAATCTTCATTAAAACTTATTGACGATTTATAT
GAAAAGTTAAATATTGAAAAACAATCAAAATAA

Upstream 100 bases:

>100_bases
AAATCATAAATATTTTATTGACTTAGGAAAAAATTTAATTCATACTAAATCGTGATAATGATTCTCATTGTCATACATCA
CGAAGGAGGCTAATTAGTCA

Downstream 100 bases:

>100_bases
TTAAGGAGTTTTACGATGCTACTTAAACCAAAATACCAAATCGTTATTGCTGGTTTATGTCTTGCAATAGTAGCTATCTT
AAGTTTAATGATTGGAAATA

Product: periplasmic binding protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVES
WTQKPKFEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQ
LTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLY
EKLNIEKQSK

Sequences:

>Translated_330_residues
MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVES
WTQKPKFEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQ
LTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLY
EKLNIEKQSK
>Mature_330_residues
MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVES
WTQKPKFEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQ
LTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLY
EKLNIEKQSK

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=283, Percent_Identity=22.2614840989399, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 36744; Mature: 36744

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLY
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEE
QGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIV
CCCHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHCEEECCCCCCCHHHHHHCCCCCEE
ASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALGKEKEAEDLLKKYDDKVAAFQ
HHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQ
HHHHCCCCCCCCCHHHHHEEECCCEEEEECCHHHHHHHHHCCCCCCCHHHHHCCCCCEEE
LTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDE
ECCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHCCCHHHCCCCCCCCHHH
ITWNLAGGYKSSLKLIDDLYEKLNIEKQSK
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLY
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEE
QGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIV
CCCHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHCEEECCCCCCCHHHHHHCCCCCEE
ASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALGKEKEAEDLLKKYDDKVAAFQ
HHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQ
HHHHCCCCCCCCCHHHHHEEECCCEEEEECCHHHHHHHHHCCCCCCCHHHHHCCCCCEEE
LTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDE
ECCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHCCCHHHCCCCCCCCHHH
ITWNLAGGYKSSLKLIDDLYEKLNIEKQSK
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]