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The map label for this gene is ecoRIIM [H]

Identifier: 148244085

GI number: 148244085

Start: 32780

End: 34243

Strand: Reverse

Name: ecoRIIM [H]

Synonym: Acry_3583

Alternate gene names: 148244085

Gene position: 34243-32780 (Counterclockwise)

Preceding gene: 148244087

Following gene: 148244084

Centisome position: 91.52

GC content: 59.43

Gene sequence:

>1464_bases
GTGCTCCGGTTAGGTGTTCGCGTTTGCCGATCGGGCATGATTTTTCCCGCTCATGATGAGCTTGAGCCGCGGGATCATGA
TCTGGTCAAGAGCCAGACTCGGGCAAAGGATATCCTTGGGGTGGCTAGCCCCTCCCTTCCGGCTCCGTTGCCTGCGAGGT
GCGAATCGGGCATAGAGTCAGAAAAATTCCCAGAGGTGACCGTGACCGAGTTTTCCATCTTGCGGCAGAAGGCAGGCTTG
TCGGTCGTGGAAGCTGCCGAGGCGCTCGGATATCACGAGCGTCACGTCTACCGGTGGGAAAATGGCAAGGGAAAGCCGCC
ACGACAGGCTGTCCTTGACTGTCTCCGGGCTCGACAGCCGGGAGGACACAAACCGGTCGCGGACACATCTGCATTCACGT
TCATCGATCTTTTCGCCGGAATCGGCGGTCTGCGGCGCGGATTCGAAGCGATCGGCGGCCGGTGCGTTTTCACGAGTGAG
TGGGACAAGTACAGCCAGGCAACCTATGCGGCCAACTATCCGTATGATGATCATACGATAAGTGGCGACATCACGAAGAT
CGGTGAGAAGGATATCCCGGAGCATGATGTCCTGCTGGCGGGCTTCCCCTGCCAGCCCTTCTCCATCGCCGGCGTCTCGA
AGAAGAATGCACTCAAACGACCGCACGGCTTTGCCTGCGAGGCCCAGGGCACGCTGTTCTTCGAGCTGGCTCGTATCATC
AAGCACCACAGGCCAAAGGTTTTCCTGCTCGAGAACGTGAAAAACCTGGTCAACCATGACCGCGGCACAACGTTCCGGAT
CATCCGCGACGTTCTCGAGAACCAGCTCGACTACAGGATCGATTTTCGTGTGATCGATGCAAAATCATGGGTTCCGCAGC
ATCGCGAGAGGATCTTCATCGCGGGTTTCCGGGACCGGAACGCGGAGTTCACCTTCAAGACGCTGGACATCCCGGATCCC
GCATCGGGTCCCCGGCTGGCCTCCATACTGCACCCGGAAGACGGAACGGAAAAACCTGACGGGCACTTTACCGAAGGCCC
ACTGGCAACCGTCACCGAGCGCTATACACTCACCGATCATCTCTGGAATTATCTGCAGAACTATGCCGAGAAGCACCGGC
GGGCCGGAAACGGCTTTGGCTTCGGCCTTTGCGGCCCGGAGGATGTCGCCCGAACCCTGTCAGCCCGCTATTACAAGGAT
GGGTCGGAGATCCTGATCCGCCAGAACGGCAAGCGCCCCCGGCGCCTGACGCCCCGCGAGTGTGCCCGTCTCATGGGCTT
CGACAGGCCTGGCGAGGCGGATTTCAAGATACCCGTGTCAGATACCCAGGCCTACAAGCAGTTCGGCAACGCGGTTGCCG
TGCCTGTAGTTGCTGCTGTCGCCCGTCACATGAAGCAATGGATTCTCGAGGCGGTCGAAGGTGACGAAAATCATCACGCC
ACCGGGCTGCAGGAGAGTGCCTGA

Upstream 100 bases:

>100_bases
CCCTCGATGAGTTGATGCTTGTCTGCGGCGAGGATCTTCACCGCCTCTTCAGGATCATCCAGCTTGAGTCCTGCGAGCGG
GCCCCAGGGAGTGTCCATCC

Downstream 100 bases:

>100_bases
TATTGTCGACCGGCAGACGCGCAGCCGGATGATGGCGGGTATCCGGTCGGCCAATACCCGACCGGAGCGGATGATCCGCA
GTGCCCTCCATGTACGTGGC

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.EcoRII; Cytosine-specific methyltransferase EcoRII [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MLRLGVRVCRSGMIFPAHDELEPRDHDLVKSQTRAKDILGVASPSLPAPLPARCESGIESEKFPEVTVTEFSILRQKAGL
SVVEAAEALGYHERHVYRWENGKGKPPRQAVLDCLRARQPGGHKPVADTSAFTFIDLFAGIGGLRRGFEAIGGRCVFTSE
WDKYSQATYAANYPYDDHTISGDITKIGEKDIPEHDVLLAGFPCQPFSIAGVSKKNALKRPHGFACEAQGTLFFELARII
KHHRPKVFLLENVKNLVNHDRGTTFRIIRDVLENQLDYRIDFRVIDAKSWVPQHRERIFIAGFRDRNAEFTFKTLDIPDP
ASGPRLASILHPEDGTEKPDGHFTEGPLATVTERYTLTDHLWNYLQNYAEKHRRAGNGFGFGLCGPEDVARTLSARYYKD
GSEILIRQNGKRPRRLTPRECARLMGFDRPGEADFKIPVSDTQAYKQFGNAVAVPVVAAVARHMKQWILEAVEGDENHHA
TGLQESA

Sequences:

>Translated_487_residues
MLRLGVRVCRSGMIFPAHDELEPRDHDLVKSQTRAKDILGVASPSLPAPLPARCESGIESEKFPEVTVTEFSILRQKAGL
SVVEAAEALGYHERHVYRWENGKGKPPRQAVLDCLRARQPGGHKPVADTSAFTFIDLFAGIGGLRRGFEAIGGRCVFTSE
WDKYSQATYAANYPYDDHTISGDITKIGEKDIPEHDVLLAGFPCQPFSIAGVSKKNALKRPHGFACEAQGTLFFELARII
KHHRPKVFLLENVKNLVNHDRGTTFRIIRDVLENQLDYRIDFRVIDAKSWVPQHRERIFIAGFRDRNAEFTFKTLDIPDP
ASGPRLASILHPEDGTEKPDGHFTEGPLATVTERYTLTDHLWNYLQNYAEKHRRAGNGFGFGLCGPEDVARTLSARYYKD
GSEILIRQNGKRPRRLTPRECARLMGFDRPGEADFKIPVSDTQAYKQFGNAVAVPVVAAVARHMKQWILEAVEGDENHHA
TGLQESA
>Mature_487_residues
MLRLGVRVCRSGMIFPAHDELEPRDHDLVKSQTRAKDILGVASPSLPAPLPARCESGIESEKFPEVTVTEFSILRQKAGL
SVVEAAEALGYHERHVYRWENGKGKPPRQAVLDCLRARQPGGHKPVADTSAFTFIDLFAGIGGLRRGFEAIGGRCVFTSE
WDKYSQATYAANYPYDDHTISGDITKIGEKDIPEHDVLLAGFPCQPFSIAGVSKKNALKRPHGFACEAQGTLFFELARII
KHHRPKVFLLENVKNLVNHDRGTTFRIIRDVLENQLDYRIDFRVIDAKSWVPQHRERIFIAGFRDRNAEFTFKTLDIPDP
ASGPRLASILHPEDGTEKPDGHFTEGPLATVTERYTLTDHLWNYLQNYAEKHRRAGNGFGFGLCGPEDVARTLSARYYKD
GSEILIRQNGKRPRRLTPRECARLMGFDRPGEADFKIPVSDTQAYKQFGNAVAVPVVAAVARHMKQWILEAVEGDENHHA
TGLQESA

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=403, Percent_Identity=50.6203473945409, Blast_Score=345, Evalue=3e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 54589; Mature: 54589

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLGVRVCRSGMIFPAHDELEPRDHDLVKSQTRAKDILGVASPSLPAPLPARCESGIES
CCCCHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCC
EKFPEVTVTEFSILRQKAGLSVVEAAEALGYHERHVYRWENGKGKPPRQAVLDCLRARQP
CCCCCEEHHHHHHHHHHHCCHHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHCCCC
GGHKPVADTSAFTFIDLFAGIGGLRRGFEAIGGRCVFTSEWDKYSQATYAANYPYDDHTI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCCCCCC
SGDITKIGEKDIPEHDVLLAGFPCQPFSIAGVSKKNALKRPHGFACEAQGTLFFELARII
CCCHHHCCCCCCCCCCEEEECCCCCCCEECCCCHHHHCCCCCCCEECCCCHHHHHHHHHH
KHHRPKVFLLENVKNLVNHDRGTTFRIIRDVLENQLDYRIDFRVIDAKSWVPQHRERIFI
HHCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEECCHHCCCHHHCEEEE
AGFRDRNAEFTFKTLDIPDPASGPRLASILHPEDGTEKPDGHFTEGPLATVTERYTLTDH
EEECCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LWNYLQNYAEKHRRAGNGFGFGLCGPEDVARTLSARYYKDGSEILIRQNGKRPRRLTPRE
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHH
CARLMGFDRPGEADFKIPVSDTQAYKQFGNAVAVPVVAAVARHMKQWILEAVEGDENHHA
HHHHHCCCCCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TGLQESA
CCCCCCC
>Mature Secondary Structure
MLRLGVRVCRSGMIFPAHDELEPRDHDLVKSQTRAKDILGVASPSLPAPLPARCESGIES
CCCCHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCC
EKFPEVTVTEFSILRQKAGLSVVEAAEALGYHERHVYRWENGKGKPPRQAVLDCLRARQP
CCCCCEEHHHHHHHHHHHCCHHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHCCCC
GGHKPVADTSAFTFIDLFAGIGGLRRGFEAIGGRCVFTSEWDKYSQATYAANYPYDDHTI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCCCCCC
SGDITKIGEKDIPEHDVLLAGFPCQPFSIAGVSKKNALKRPHGFACEAQGTLFFELARII
CCCHHHCCCCCCCCCCEEEECCCCCCCEECCCCHHHHCCCCCCCEECCCCHHHHHHHHHH
KHHRPKVFLLENVKNLVNHDRGTTFRIIRDVLENQLDYRIDFRVIDAKSWVPQHRERIFI
HHCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEECCHHCCCHHHCEEEE
AGFRDRNAEFTFKTLDIPDPASGPRLASILHPEDGTEKPDGHFTEGPLATVTERYTLTDH
EEECCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LWNYLQNYAEKHRRAGNGFGFGLCGPEDVARTLSARYYKDGSEILIRQNGKRPRRLTPRE
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHH
CARLMGFDRPGEADFKIPVSDTQAYKQFGNAVAVPVVAAVARHMKQWILEAVEGDENHHA
HHHHHCCCCCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TGLQESA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029675 [H]