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The map label for this gene is istB [H]

Identifier: 148243756

GI number: 148243756

Start: 149080

End: 149886

Strand: Reverse

Name: istB [H]

Synonym: Acry_3234

Alternate gene names: 148243756

Gene position: 149886-149080 (Counterclockwise)

Preceding gene: 148243757

Following gene: 148243755

Centisome position: 73.62

GC content: 61.83

Gene sequence:

>807_bases
ATGCGTCATGATCCCGCCGCGGGTGCGCTCGTCATCATGCTTCGCAGCCTCAAAATGCACGGCATGGCACAGGCCGTCGA
TGAACTGGCCCAGCAGGGTGCTCCGGCCTTTGAAGCCGCCATCCCGGTGCTCTCCCAGTTGCTCAAGGCCGAGACCGCTG
AGCGCGAGGTCCGCTCCATGGCCTATCAGCTCAAGGCCGCCCGCTTCCCGGCCTATCGCGACCTGGCCGGCTTCGACTTC
GCCAGCAGCGAGGTCAATGAGGCCCTGGTCAGGCAGTTGCATCGTTGCGACTTCATCGAGCCCGCCGACAATGTCGTGCT
CGTCGGCGGCCCGGGCACGGGCAAAACCCACATCGCCACCGCGCTTGGCGTGCAGGCCGTCGAGCATCACAAAAAGCGCA
TCCGCTTCTTCTCCACCGTCGAGCTGGTCAATGCTCTGGAGCAGGAAAAGGCTCAGGGAAAGGCCGGGCAAATCGCCGGC
CGCCTGATCCATGCCGACCTCGTCATCCTCGATGAGCTTGGCTATCTGCCCTTCAGCGCCTCAGGCGGCGCTTTGCTGTT
CCATCTGCTCAGCAAGCTTTACGAGCGCACCAGCGTCGCCATCACCACCAACCTCAGCTTCAGCGAATGGGCCGCGGTCT
TCGGCGACGCCAAGATGACCACCGCTCTCCTCGACCGCCTGACGCACCACTGCCACATCCTGGAGACCGGCAATGACAGC
TTCCGGTTCAAGAACAGCTCAGTGCAGAAATCTCAAAACGAAAGGAGAAAAGCAGCCCTTGACCCCATCATGAAACCCAA
ATCATAA

Upstream 100 bases:

>100_bases
CCGCCAGTCATCACCGCACCACAGGCGTTGACATTGGCCCATGAGCCCAGGGCCGATGTCGAGCGCTACGATACCCTGCG
TCAAACCGGGGAGACTCGCC

Downstream 100 bases:

>100_bases
CCAAAAGGCGGGTCAAATCTCGATGGAATTCCCGGGTCACTTCTCAGTGGAAATCAACAGGGTCCGCCTCTCGCCCTTGG
CGCCGACTGGTTCACCTGGA

Product: IstB ATP binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRHDPAAGALVIMLRSLKMHGMAQAVDELAQQGAPAFEAAIPVLSQLLKAETAEREVRSMAYQLKAARFPAYRDLAGFDF
ASSEVNEALVRQLHRCDFIEPADNVVLVGGPGTGKTHIATALGVQAVEHHKKRIRFFSTVELVNALEQEKAQGKAGQIAG
RLIHADLVILDELGYLPFSASGGALLFHLLSKLYERTSVAITTNLSFSEWAAVFGDAKMTTALLDRLTHHCHILETGNDS
FRFKNSSVQKSQNERRKAALDPIMKPKS

Sequences:

>Translated_268_residues
MRHDPAAGALVIMLRSLKMHGMAQAVDELAQQGAPAFEAAIPVLSQLLKAETAEREVRSMAYQLKAARFPAYRDLAGFDF
ASSEVNEALVRQLHRCDFIEPADNVVLVGGPGTGKTHIATALGVQAVEHHKKRIRFFSTVELVNALEQEKAQGKAGQIAG
RLIHADLVILDELGYLPFSASGGALLFHLLSKLYERTSVAITTNLSFSEWAAVFGDAKMTTALLDRLTHHCHILETGNDS
FRFKNSSVQKSQNERRKAALDPIMKPKS
>Mature_268_residues
MRHDPAAGALVIMLRSLKMHGMAQAVDELAQQGAPAFEAAIPVLSQLLKAETAEREVRSMAYQLKAARFPAYRDLAGFDF
ASSEVNEALVRQLHRCDFIEPADNVVLVGGPGTGKTHIATALGVQAVEHHKKRIRFFSTVELVNALEQEKAQGKAGQIAG
RLIHADLVILDELGYLPFSASGGALLFHLLSKLYERTSVAITTNLSFSEWAAVFGDAKMTTALLDRLTHHCHILETGNDS
FRFKNSSVQKSQNERRKAALDPIMKPKS

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 29343; Mature: 29343

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHDPAAGALVIMLRSLKMHGMAQAVDELAQQGAPAFEAAIPVLSQLLKAETAEREVRSM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AYQLKAARFPAYRDLAGFDFASSEVNEALVRQLHRCDFIEPADNVVLVGGPGTGKTHIAT
HHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHH
ALGVQAVEHHKKRIRFFSTVELVNALEQEKAQGKAGQIAGRLIHADLVILDELGYLPFSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
SGGALLFHLLSKLYERTSVAITTNLSFSEWAAVFGDAKMTTALLDRLTHHCHILETGNDS
CCHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHEEEEECCCCC
FRFKNSSVQKSQNERRKAALDPIMKPKS
EEECCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRHDPAAGALVIMLRSLKMHGMAQAVDELAQQGAPAFEAAIPVLSQLLKAETAEREVRSM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AYQLKAARFPAYRDLAGFDFASSEVNEALVRQLHRCDFIEPADNVVLVGGPGTGKTHIAT
HHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHH
ALGVQAVEHHKKRIRFFSTVELVNALEQEKAQGKAGQIAGRLIHADLVILDELGYLPFSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
SGGALLFHLLSKLYERTSVAITTNLSFSEWAAVFGDAKMTTALLDRLTHHCHILETGNDS
CCHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHEEEEECCCCC
FRFKNSSVQKSQNERRKAALDPIMKPKS
EEECCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]