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The map label for this gene is fixK [H]

Identifier: 148243737

GI number: 148243737

Start: 123843

End: 124535

Strand: Reverse

Name: fixK [H]

Synonym: Acry_3214

Alternate gene names: 148243737

Gene position: 124535-123843 (Counterclockwise)

Preceding gene: 148243740

Following gene: 148243735

Centisome position: 61.17

GC content: 50.94

Gene sequence:

>693_bases
ATGGGTCGCATTTTGGTCGAAGCTCTGGAGTTTCAGGCTGCAATTCGGACGACCTGCGGTCAGCTAGGTTATCACGCACG
AGCGCTTGAAGCCGGCACTACCATCTTCATGGAAAATCAAATTGCGGACAAGATGTACCAAATTGTTTCTGGTATCGTTC
GACTTTCAAAGGGCTTGCAAGACGGACGACGGCAAGTGTTAGGATTCCTCTTCGCCGGCGATTTTTTCGTGCCATCCGGA
TTTGTAGTTGGCATTAACAAAAATGCAACCTGTACGGCAGAAGCCCTAACTGATATGCAGATCATCAGCCTGCCGCTTCG
AAATCTTGATGCTTTGCTGACCGATCAGCCCAAGTTTGCAAGCGACCTGCTTTTAGCAACTGAACGTAGCCTGATACAGG
CGCAACAACAGATGCTGCGGCTTGGCCGTATGTCTGCAACAGAACGAGTGACCTGGTTCCTCCTCATGATGAAGGAACAG
CAAGACGATGACGGCGACGCGTCGATCCATCTGCCGATGTCTCGCATTGATATTGCCGATTATCTCGGAATAAGGAGCGA
AACCATATCAAGGGTCCTTCGGTGGCTTCGTGACCATCGCATGATCACAGTCAGCGACCGCGCCACAATCACCATCATTG
ACTCCGCAAGCATGTGCCATGTCTCCGGTGGATTCTATCCTAGTCGTCTGTGA

Upstream 100 bases:

>100_bases
AACAAACTGCGGCAAAATAACATTTATCTGTTACAATTATTAGATATGAAGTCCCGATTAAATTTATAGACCTGATCTCA
AAATAGGAGACGGCACGCTA

Downstream 100 bases:

>100_bases
ATTAAGCGATGTGGTAGTTCGGTAGGCGAAGGTCTGGAGAGGGTCTTGCGGCCGAGAGCCATGTGAAGATGGCGCAAAGA
AGCCTGATCAGCCATCTGCG

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MGRILVEALEFQAAIRTTCGQLGYHARALEAGTTIFMENQIADKMYQIVSGIVRLSKGLQDGRRQVLGFLFAGDFFVPSG
FVVGINKNATCTAEALTDMQIISLPLRNLDALLTDQPKFASDLLLATERSLIQAQQQMLRLGRMSATERVTWFLLMMKEQ
QDDDGDASIHLPMSRIDIADYLGIRSETISRVLRWLRDHRMITVSDRATITIIDSASMCHVSGGFYPSRL

Sequences:

>Translated_230_residues
MGRILVEALEFQAAIRTTCGQLGYHARALEAGTTIFMENQIADKMYQIVSGIVRLSKGLQDGRRQVLGFLFAGDFFVPSG
FVVGINKNATCTAEALTDMQIISLPLRNLDALLTDQPKFASDLLLATERSLIQAQQQMLRLGRMSATERVTWFLLMMKEQ
QDDDGDASIHLPMSRIDIADYLGIRSETISRVLRWLRDHRMITVSDRATITIIDSASMCHVSGGFYPSRL
>Mature_229_residues
GRILVEALEFQAAIRTTCGQLGYHARALEAGTTIFMENQIADKMYQIVSGIVRLSKGLQDGRRQVLGFLFAGDFFVPSGF
VVGINKNATCTAEALTDMQIISLPLRNLDALLTDQPKFASDLLLATERSLIQAQQQMLRLGRMSATERVTWFLLMMKEQQ
DDDGDASIHLPMSRIDIADYLGIRSETISRVLRWLRDHRMITVSDRATITIIDSASMCHVSGGFYPSRL

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=199, Percent_Identity=26.1306532663317, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25704; Mature: 25573

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRILVEALEFQAAIRTTCGQLGYHARALEAGTTIFMENQIADKMYQIVSGIVRLSKGLQ
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
DGRRQVLGFLFAGDFFVPSGFVVGINKNATCTAEALTDMQIISLPLRNLDALLTDQPKFA
HHHHHHHHHHHHCCEECCCCEEEEECCCCEEHHHHHHHHHHHHHCHHHHHHHHCCCCCHH
SDLLLATERSLIQAQQQMLRLGRMSATERVTWFLLMMKEQQDDDGDASIHLPMSRIDIAD
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEHHHCCCCCCCCEEEECHHHHHHHH
YLGIRSETISRVLRWLRDHRMITVSDRATITIIDSASMCHVSGGFYPSRL
HHCCCHHHHHHHHHHHHHCCEEEEECCEEEEEEECCCEEEECCCCCCCCC
>Mature Secondary Structure 
GRILVEALEFQAAIRTTCGQLGYHARALEAGTTIFMENQIADKMYQIVSGIVRLSKGLQ
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
DGRRQVLGFLFAGDFFVPSGFVVGINKNATCTAEALTDMQIISLPLRNLDALLTDQPKFA
HHHHHHHHHHHHCCEECCCCEEEEECCCCEEHHHHHHHHHHHHHCHHHHHHHHCCCCCHH
SDLLLATERSLIQAQQQMLRLGRMSATERVTWFLLMMKEQQDDDGDASIHLPMSRIDIAD
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEHHHCCCCCCCCEEEECHHHHHHHH
YLGIRSETISRVLRWLRDHRMITVSDRATITIIDSASMCHVSGGFYPSRL
HHCCCHHHHHHHHHHHHHCCEEEEECCEEEEEEECCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2663474; 11481432 [H]