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The map label for this gene is irlS [H]

Identifier: 148243638

GI number: 148243638

Start: 2918

End: 4261

Strand: Direct

Name: irlS [H]

Synonym: Acry_3107

Alternate gene names: 148243638

Gene position: 2918-4261 (Clockwise)

Preceding gene: 148243637

Following gene: 148243639

Centisome position: 1.43

GC content: 64.43

Gene sequence:

>1344_bases
ATGTCCCTGCGGATGACCGCGTGGTATGCAGGGACGGCGTTCCTGCTCGTCCTGCTGGCCACTAGTCTCCTGACCTGGTC
GTTTGGCGCGCGCATCGCCAGGCAGGAAGACCGCACGCTGCACGTGCTCGCACAGATCGCCCGCCCGCTGGGCGGCGCTG
CCGATCCGTCCGCATCGCAGGCCATTGCATCGGCGCTTGAGCGAGCGGGCAACACCCGCGAGATCCAGTTCCGCGTCATC
GGCCCGCACGGTCGGATCACAATCCAGTCAAAGGGCAATCATCTGCCTCTGCCATCATCGGTCGGCTTGCCGAACGTCGC
CACGCTTGATAGCGGCGGGATGGCGTTTCGTGTTCTCACCCAGCCCCTGCCGAACGGCTTCATGCTGCAGATCGCCATCG
CCAGCCCCGGCTCGCAGGCCCTCCTGCTCCGTTACCGCGAAACCCTTACCCTGATTCTTGCGGTGTCGATCATCCTCTGC
GCCGTGCTCGGATATCTCATCGCCCGTTCGGGCATGCGCCCGATCGAGCGCATTGCCGAAACGGCCGAAAACATCCGCCT
TTCGACCCTGCATGAACGTCTCGACGTCGCAGGGCTTCCCGCCGAACTCGCCGCGCTCGCCAACACGTTCAACCTGATGT
TGGACCGGCTTGAGCAATCCTTCCTCCAGCTCTCCCAATTCTCGGCTGACCTCGCGCACGAGCTGCGCACGCCGATCAGC
AATCTGCGCGGCGAGCTGGAAGTTGCGTTATCGAGGCCCCGCTCTGACGATACTTATCGGGAAGTCCTCGGCTCCGCACT
CGAGGAATGCACTCGCATCAGCCGGGTCATTCAGAGCCTCCTGTTCCTTGCCCGCGCCGAGATGGTCGGGGCTCCACCCG
CCGTTGAAGCTCTTGATCTTGCCAGCGAGATCGCAACGGTTCTCGAATTCTACCAGCCGATGGCGGCGGAGGCGGGGCTG
TCGCTGACCGCCCGCGTCCGCCCGGGACTACCGATCAGGTTCGACCGCATTCTCCTCCAGCAGGCGCTCGGCAATCTGGT
GGCCAATGCAATCGCCCATACCGGCACCGGCGGGCAGATCACCATCGAAGTTGCGGAGGTGCTGGAACAGCTCTCCATCC
GCATAAAGGATACCGGGCAGGGGATCGCACCCGAGCATCTGCCGCATGTGTTCGACCGGTTCTATCGCGCCGATCCCGCG
CGTTCGCATGTCGGCGGCAATCTCGGGCTCGGCCTCGCCGTGGTTCGCGCGATCATGGACCTGCACGGGGGCCGGGTTTC
CATTGAAAGCACGCAGGGTCAGGGCACCACCGTATCGCTACTGGTGGCAGCGACCGCGCGCTAA

Upstream 100 bases:

>100_bases
GACCCGTTCTCCCGCCGCCTGATCCACACCGTTCGTGGCGTGGGTTACGTGCTCGAAGACTGTGGCGGCTGATCCTGAAG
CGCCGAGCGCTGGCGGATCG

Downstream 100 bases:

>100_bases
TCCCGAAACGGTGAGCCCAAACTGACGAAATCGTCATTCTGGCGAGGCGGAAACGTCATTCTCGCCGGCTAAAGCGCGAA
CTCTTTTCCGCTTGAAGCCA

Product: heavy metal sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSLRMTAWYAGTAFLLVLLATSLLTWSFGARIARQEDRTLHVLAQIARPLGGAADPSASQAIASALERAGNTREIQFRVI
GPHGRITIQSKGNHLPLPSSVGLPNVATLDSGGMAFRVLTQPLPNGFMLQIAIASPGSQALLLRYRETLTLILAVSIILC
AVLGYLIARSGMRPIERIAETAENIRLSTLHERLDVAGLPAELAALANTFNLMLDRLEQSFLQLSQFSADLAHELRTPIS
NLRGELEVALSRPRSDDTYREVLGSALEECTRISRVIQSLLFLARAEMVGAPPAVEALDLASEIATVLEFYQPMAAEAGL
SLTARVRPGLPIRFDRILLQQALGNLVANAIAHTGTGGQITIEVAEVLEQLSIRIKDTGQGIAPEHLPHVFDRFYRADPA
RSHVGGNLGLGLAVVRAIMDLHGGRVSIESTQGQGTTVSLLVAATAR

Sequences:

>Translated_447_residues
MSLRMTAWYAGTAFLLVLLATSLLTWSFGARIARQEDRTLHVLAQIARPLGGAADPSASQAIASALERAGNTREIQFRVI
GPHGRITIQSKGNHLPLPSSVGLPNVATLDSGGMAFRVLTQPLPNGFMLQIAIASPGSQALLLRYRETLTLILAVSIILC
AVLGYLIARSGMRPIERIAETAENIRLSTLHERLDVAGLPAELAALANTFNLMLDRLEQSFLQLSQFSADLAHELRTPIS
NLRGELEVALSRPRSDDTYREVLGSALEECTRISRVIQSLLFLARAEMVGAPPAVEALDLASEIATVLEFYQPMAAEAGL
SLTARVRPGLPIRFDRILLQQALGNLVANAIAHTGTGGQITIEVAEVLEQLSIRIKDTGQGIAPEHLPHVFDRFYRADPA
RSHVGGNLGLGLAVVRAIMDLHGGRVSIESTQGQGTTVSLLVAATAR
>Mature_446_residues
SLRMTAWYAGTAFLLVLLATSLLTWSFGARIARQEDRTLHVLAQIARPLGGAADPSASQAIASALERAGNTREIQFRVIG
PHGRITIQSKGNHLPLPSSVGLPNVATLDSGGMAFRVLTQPLPNGFMLQIAIASPGSQALLLRYRETLTLILAVSIILCA
VLGYLIARSGMRPIERIAETAENIRLSTLHERLDVAGLPAELAALANTFNLMLDRLEQSFLQLSQFSADLAHELRTPISN
LRGELEVALSRPRSDDTYREVLGSALEECTRISRVIQSLLFLARAEMVGAPPAVEALDLASEIATVLEFYQPMAAEAGLS
LTARVRPGLPIRFDRILLQQALGNLVANAIAHTGTGGQITIEVAEVLEQLSIRIKDTGQGIAPEHLPHVFDRFYRADPAR
SHVGGNLGLGLAVVRAIMDLHGGRVSIESTQGQGTTVSLLVAATAR

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=285, Percent_Identity=39.6491228070175, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788279, Length=317, Percent_Identity=34.7003154574132, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1786600, Length=282, Percent_Identity=31.2056737588652, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790346, Length=262, Percent_Identity=30.5343511450382, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=27.7580071174377, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790436, Length=228, Percent_Identity=28.5087719298246, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1790861, Length=391, Percent_Identity=27.3657289002558, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788549, Length=221, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=26.2008733624454, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789149, Length=345, Percent_Identity=24.9275362318841, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787894, Length=235, Percent_Identity=29.3617021276596, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=201, Percent_Identity=31.3432835820896, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789808, Length=288, Percent_Identity=27.0833333333333, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789403, Length=285, Percent_Identity=27.0175438596491, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788713, Length=224, Percent_Identity=25.4464285714286, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI48994928, Length=238, Percent_Identity=26.0504201680672, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787374, Length=284, Percent_Identity=26.7605633802817, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48005; Mature: 47874

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50885 HAMP ; PS00041 HTH_ARAC_FAMILY_1 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRMTAWYAGTAFLLVLLATSLLTWSFGARIARQEDRTLHVLAQIARPLGGAADPSASQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHH
AIASALERAGNTREIQFRVIGPHGRITIQSKGNHLPLPSSVGLPNVATLDSGGMAFRVLT
HHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEEC
QPLPNGFMLQIAIASPGSQALLLRYRETLTLILAVSIILCAVLGYLIARSGMRPIERIAE
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TAENIRLSTLHERLDVAGLPAELAALANTFNLMLDRLEQSFLQLSQFSADLAHELRTPIS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
NLRGELEVALSRPRSDDTYREVLGSALEECTRISRVIQSLLFLARAEMVGAPPAVEALDL
HHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ASEIATVLEFYQPMAAEAGLSLTARVRPGLPIRFDRILLQQALGNLVANAIAHTGTGGQI
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TIEVAEVLEQLSIRIKDTGQGIAPEHLPHVFDRFYRADPARSHVGGNLGLGLAVVRAIMD
EEHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHH
LHGGRVSIESTQGQGTTVSLLVAATAR
HCCCEEEEECCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
SLRMTAWYAGTAFLLVLLATSLLTWSFGARIARQEDRTLHVLAQIARPLGGAADPSASQ
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHH
AIASALERAGNTREIQFRVIGPHGRITIQSKGNHLPLPSSVGLPNVATLDSGGMAFRVLT
HHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEEC
QPLPNGFMLQIAIASPGSQALLLRYRETLTLILAVSIILCAVLGYLIARSGMRPIERIAE
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TAENIRLSTLHERLDVAGLPAELAALANTFNLMLDRLEQSFLQLSQFSADLAHELRTPIS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
NLRGELEVALSRPRSDDTYREVLGSALEECTRISRVIQSLLFLARAEMVGAPPAVEALDL
HHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ASEIATVLEFYQPMAAEAGLSLTARVRPGLPIRFDRILLQQALGNLVANAIAHTGTGGQI
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TIEVAEVLEQLSIRIKDTGQGIAPEHLPHVFDRFYRADPARSHVGGNLGLGLAVVRAIMD
EEHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHH
LHGGRVSIESTQGQGTTVSLLVAATAR
HCCCEEEEECCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]