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The map label for this gene is pepB [H]

Identifier: 147675760

GI number: 147675760

Start: 297139

End: 298473

Strand: Direct

Name: pepB [H]

Synonym: VC0395_A0284

Alternate gene names: 147675760

Gene position: 297139-298473 (Clockwise)

Preceding gene: 147674974

Following gene: 147673453

Centisome position: 9.83

GC content: 50.94

Gene sequence:

>1335_bases
ATGAGATGGCACAGGCAAAGCCAATATTTAAGACAAGGAGAAACCATGTCTACACAGATGTCTGTATTTTTGAGTACTCA
AGCTGCCCAGCCTCAGTGGGGAGAGAAAGCGCTCATTTCTTTTGCAGAGCAAGGTGCAACCATTCACCTGCAACAAACAC
AAGATTTCAGCGCAATCCAACGCGCAGCTCGTAAGTTAGATAATCAAGGTATCCGCACGGCATTCCTGGCTGGAGAAGGT
TGGGATCTGGAGAGCATTTGGGCTTTCTATCAAGGCTACCGTGATGCGAAAAAGCGCAATACCGTCGAGTGGAAAGCGTT
AGCGGCTGCTGAACAAGCAGAGCTAGAAGCGCGCATTAAAGCGACGGATTGGACACGCGATATCATCAACAAAAGCGCGG
AAGAAGTCGCGCCACGCCAATTGGCGACCATGGCAGCAGAGTTCATCAAATCGCTTGCGCCTGACCATGTTTCTTACCGT
ATCGTCAAAGACAAAGATCTGCTCACTGAAGGGTGGGAGGGGATTTACGCTGTAGGCCGTGGCTCTGAGCGTACGTCGGC
GATGCTGCAACTCGACTACAACCCAACGGGCGATGAAAATGCACCGGTATTCGCGTGTTTAGTCGGTAAAGGCATCACTT
TTGACTCGGGTGGTTACAGCTTAAAACCTTCCAACATGATGTCAGCGATGAAAGCGGACATGGGCGGCTCAGGCATGATC
ACTGGTGCGCTTGGTTTGGCTATCATGCGCGGCTTTAACAAGCGCGTGAAACTCATTCTATGCTGCGCGGAAAACATGGT
TTCCGGCCGTGCGTTGAAGCTTGGTGACATCATCACCTACAAAAATGGCAAAACCGTTGAAATCATGAACACCGATGCGG
AAGGCCGTTTGGTGCTGGCCGACGGTCTTATCTACGCCAGTGAACAGAAACCGCAATTGATTATCGACTGTGCAACCTTA
ACCGGAGCGGCGAAAAACGCGCTGGGTAATGATTACCACGCACTGCTTTCTTATGATGAGTCGCTGAGCCAACAAGCATT
ATCTGCGGCAAAAGAAGAGAATGAAGCGCTGTGGGCTCTGCCTTTAGCTGAGTTCCACCGTGAAATGCTGCCTTCTAACT
TTGCGGATCTGTCAAACATCAGTAACGGCGATTACACGCCGGGAGCCAGCACCGCAGCGGCCTTCCTTTCCTATTTCGTG
GAAGGCTACCAAAAAGGTTGGCTACACTTCGATTGTTCAGCCACGTATCGCAAGTCAGCCAGCGATAAATGGGCTGCAGG
AGCCACGGGCATGGGCGTGAAAATGCTCGCACGTATTTTGATGCAGCAAGCATAA

Upstream 100 bases:

>100_bases
CAGAGTTAACACTTCTTAGCAAAAAATGCGGACCACTTGGTCCGTTTTTTTATTCGGTTGACATTTTGCCTCGACATAAT
GCTAACATCTTGGCGATTTA

Downstream 100 bases:

>100_bases
ATTTAAAGGCAGCTTGTATAGCTGCCTTGTTTTTATTAATAAAAGTATACCCTTCCAATTTGACGCTGCAGCGGTGTTGG
CTCTGTTCGTTCATCCTAAT

Product: aminopeptidase B

Products: NA

Alternate protein names: Aminopeptidase B [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRWHRQSQYLRQGETMSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQRAARKLDNQGIRTAFLAGEG
WDLESIWAFYQGYRDAKKRNTVEWKALAAAEQAELEARIKATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYR
IVKDKDLLTEGWEGIYAVGRGSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMI
TGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLADGLIYASEQKPQLIIDCATL
TGAAKNALGNDYHALLSYDESLSQQALSAAKEENEALWALPLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFV
EGYQKGWLHFDCSATYRKSASDKWAAGATGMGVKMLARILMQQA

Sequences:

>Translated_444_residues
MRWHRQSQYLRQGETMSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQRAARKLDNQGIRTAFLAGEG
WDLESIWAFYQGYRDAKKRNTVEWKALAAAEQAELEARIKATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYR
IVKDKDLLTEGWEGIYAVGRGSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMI
TGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLADGLIYASEQKPQLIIDCATL
TGAAKNALGNDYHALLSYDESLSQQALSAAKEENEALWALPLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFV
EGYQKGWLHFDCSATYRKSASDKWAAGATGMGVKMLARILMQQA
>Mature_444_residues
MRWHRQSQYLRQGETMSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQRAARKLDNQGIRTAFLAGEG
WDLESIWAFYQGYRDAKKRNTVEWKALAAAEQAELEARIKATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYR
IVKDKDLLTEGWEGIYAVGRGSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMI
TGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLADGLIYASEQKPQLIIDCATL
TGAAKNALGNDYHALLSYDESLSQQALSAAKEENEALWALPLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFV
EGYQKGWLHFDCSATYRKSASDKWAAGATGMGVKMLARILMQQA

Specific function: Probably plays an important role in intracellular peptide degradation [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=321, Percent_Identity=35.202492211838, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI47155554, Length=353, Percent_Identity=32.2946175637394, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI87082123, Length=425, Percent_Identity=60, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI1790710, Length=305, Percent_Identity=39.344262295082, Blast_Score=199, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17556903, Length=259, Percent_Identity=36.6795366795367, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17565172, Length=241, Percent_Identity=29.8755186721992, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357381, Length=343, Percent_Identity=31.7784256559767, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI221379063, Length=343, Percent_Identity=31.7784256559767, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI221379062, Length=343, Percent_Identity=31.7784256559767, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355725, Length=354, Percent_Identity=31.9209039548023, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24661038, Length=354, Percent_Identity=31.0734463276836, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129969, Length=372, Percent_Identity=27.9569892473118, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24662227, Length=368, Percent_Identity=26.6304347826087, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI161077148, Length=411, Percent_Identity=26.5206812652068, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI20130057, Length=411, Percent_Identity=26.5206812652068, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI20129963, Length=368, Percent_Identity=27.1739130434783, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI19922386, Length=405, Percent_Identity=25.679012345679, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21355645, Length=351, Percent_Identity=25.6410256410256, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24662223, Length=351, Percent_Identity=25.6410256410256, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24646701, Length=211, Percent_Identity=28.9099526066351, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646703, Length=211, Percent_Identity=28.9099526066351, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358201, Length=211, Percent_Identity=28.9099526066351, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008330
- InterPro:   IPR011356
- InterPro:   IPR000819 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.23 [H]

Molecular weight: Translated: 48706; Mature: 48706

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWHRQSQYLRQGETMSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQ
CCCCHHHHHHHCCCCHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHH
RAARKLDNQGIRTAFLAGEGWDLESIWAFYQGYRDAKKRNTVEWKALAAAEQAELEARIK
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEE
ATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYRIVKDKDLLTEGWEGIYAVGR
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCEEEECC
GSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMI
CCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCCEECHHHHHHHHHHCCCCCCCH
TGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLA
HHHHHHHHHHCCCCCEEEEEEEHHHHCCCCEEEECCEEEECCCCEEEEEECCCCCEEEEE
DGLIYASEQKPQLIIDCATLTGAAKNALGNDYHALLSYDESLSQQALSAAKEENEALWAL
CCEEEECCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
PLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFVEGYQKGWLHFDCSATYRKSA
CHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCC
SDKWAAGATGMGVKMLARILMQQA
CCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRWHRQSQYLRQGETMSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQ
CCCCHHHHHHHCCCCHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHH
RAARKLDNQGIRTAFLAGEGWDLESIWAFYQGYRDAKKRNTVEWKALAAAEQAELEARIK
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEE
ATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYRIVKDKDLLTEGWEGIYAVGR
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCEEEECC
GSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMI
CCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCCEECHHHHHHHHHHCCCCCCCH
TGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLA
HHHHHHHHHHCCCCCEEEEEEEHHHHCCCCEEEECCEEEECCCCEEEEEECCCCCEEEEE
DGLIYASEQKPQLIIDCATLTGAAKNALGNDYHALLSYDESLSQQALSAAKEENEALWAL
CCEEEECCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
PLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFVEGYQKGWLHFDCSATYRKSA
CHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCC
SDKWAAGATGMGVKMLARILMQQA
CCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]