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The map label for this gene is ycdT [H]

Identifier: 147675568

GI number: 147675568

Start: 1993077

End: 1994594

Strand: Reverse

Name: ycdT [H]

Synonym: VC0395_A1874

Alternate gene names: 147675568

Gene position: 1994594-1993077 (Counterclockwise)

Preceding gene: 147674779

Following gene: 147673593

Centisome position: 65.96

GC content: 44.6

Gene sequence:

>1518_bases
ATGAATTTAAATAACTTTAGCCTACGCTGGCTAACGACACTCAATGCGTTAGCGGTGGTGTTAGGCTTTTTAATGTTTTA
TCTGACCTTTAAATATTTCTGGTCACATGATCGTGAAGTTGCGCAAGTGCTGCAGTTACAACAGGCCGAGTTGCAGCGGG
TTGAAACCTTGTTGTCGTTAGAACGTAAGGCGATGGGGGCTTCTTTGGCAGATTATGCCGCTTGGGATGAAATGGCAGAC
TTCATTGCAAAACCCACCCTTGAGTTCACACAAAGCAATATTGGAGAGCACGCTTTCAGTTCACAATTCCTCGATGGCGT
ATTTATCTATGATCCTGAGGGCAATTTGGTTTGGGGCAAGAAATATGACGCAGCAACGGGGCAGAGCTCTAGCTATGAGC
ACTTATTGCCGGATTTTTCACGTATTTTACAGCAGGCGACACGTTTAAGTGTGGATGAAATCTCGACTTCCGTTCGTTAT
ATGGTGGTGGAGGATGAGCCCTATTTAGCTGCCACGGCTCGTGTCTGTGACAGTGATGGGAAAGGGTGTAACAAAGGTTT
CTTAATCTTTATTAAAAAAGTCCGCGCCCAGTTTGCGAATGTGGTTGAGCAAGCCACTGGTGTCGATATTGAAGTCTTAA
CCTGTAAAAATGATGCGCCCTTACCGCAAGACGAAGTGGATGTTTCATACATCAAGCAACTCGATTACAGCGGTAATTCC
AGTGTGTTGTTTAAAATTAACCATCACATTAAGCATCCTCCCTTCATTCGAACCGAAGAAATTCTTGCTTTGCTGTTCTT
TTCACTGGTGATGTACTTAGTCAATTTATGGGTGGTGATTGCCTTGATCAAACCGATCACGACCGCCAGTCAAGTGTTGC
AACAATTTAAAACATCGGGCGGAAAAATGCCGGATGCGAGTACTTTTATCTCATCGGAGATGAAAGAATTTGCCACTACG
ATCAACCGAATCGTCGGACAATTGGAGGATAGTCAACAAGTGTTACGTTGGCAGTCTGAGCATGATCCACTAACCCGCAT
TTCCAATCGTCGCCATTTAGAAAAGCAGCTTAAAAGTTATTTGAGCGATCGTCCGCAAGCCTATCTGGTGCTGTTTCTTG
TCGATATCGATTTTTTCAAACGTTTCAATGACAGCTTTGGTCATTTGGCTGGCGATGAAGCCTTATGTTCCGTTGCTGAT
GTGCTCCAATCGGTGGAGTTCCATGGCGAGAAAATCGTGGCGCGCTTTGGGGGAGAAGAGTTTTGTGTTGTTTTAGCTTC
GGACTGTGCATTTGATGCAGAACAGTATGCACAACAGATGAGAAGCAAAATCGAGCAGCTCGCGATTGCAAATCCGGTTG
ATGCACTCTGCCAATATCTAACGGTGAGCATTGGTGGTGTGTATGCTATTTCTCCAAAGATGGAGTCTTATCTCTCTTTG
TTCCATCAGGCGGATATGGCACTTTATCATGCGAAAGAACACGGACGAGATCGATACGTTGTGCGAAATTTTGTCTAA

Upstream 100 bases:

>100_bases
CCCTCAACTAAAGAACTATCAAGTCTTCACCATCTAAACTGATAAGAGCGTGACCTGCGCTCTTTTGTTCTAAGTAAAGG
TTTATTATGACGATGGTGAT

Downstream 100 bases:

>100_bases
ATTTTAAACGATACATCATTTGAAATTTGATTTGAGTCGATAAAAGTAAAGTGATTGGGTGATTTTTCAGCGAATGATTC
TGTTGCTGATTGAGTCAATC

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNLNNFSLRWLTTLNALAVVLGFLMFYLTFKYFWSHDREVAQVLQLQQAELQRVETLLSLERKAMGASLADYAAWDEMAD
FIAKPTLEFTQSNIGEHAFSSQFLDGVFIYDPEGNLVWGKKYDAATGQSSSYEHLLPDFSRILQQATRLSVDEISTSVRY
MVVEDEPYLAATARVCDSDGKGCNKGFLIFIKKVRAQFANVVEQATGVDIEVLTCKNDAPLPQDEVDVSYIKQLDYSGNS
SVLFKINHHIKHPPFIRTEEILALLFFSLVMYLVNLWVVIALIKPITTASQVLQQFKTSGGKMPDASTFISSEMKEFATT
INRIVGQLEDSQQVLRWQSEHDPLTRISNRRHLEKQLKSYLSDRPQAYLVLFLVDIDFFKRFNDSFGHLAGDEALCSVAD
VLQSVEFHGEKIVARFGGEEFCVVLASDCAFDAEQYAQQMRSKIEQLAIANPVDALCQYLTVSIGGVYAISPKMESYLSL
FHQADMALYHAKEHGRDRYVVRNFV

Sequences:

>Translated_505_residues
MNLNNFSLRWLTTLNALAVVLGFLMFYLTFKYFWSHDREVAQVLQLQQAELQRVETLLSLERKAMGASLADYAAWDEMAD
FIAKPTLEFTQSNIGEHAFSSQFLDGVFIYDPEGNLVWGKKYDAATGQSSSYEHLLPDFSRILQQATRLSVDEISTSVRY
MVVEDEPYLAATARVCDSDGKGCNKGFLIFIKKVRAQFANVVEQATGVDIEVLTCKNDAPLPQDEVDVSYIKQLDYSGNS
SVLFKINHHIKHPPFIRTEEILALLFFSLVMYLVNLWVVIALIKPITTASQVLQQFKTSGGKMPDASTFISSEMKEFATT
INRIVGQLEDSQQVLRWQSEHDPLTRISNRRHLEKQLKSYLSDRPQAYLVLFLVDIDFFKRFNDSFGHLAGDEALCSVAD
VLQSVEFHGEKIVARFGGEEFCVVLASDCAFDAEQYAQQMRSKIEQLAIANPVDALCQYLTVSIGGVYAISPKMESYLSL
FHQADMALYHAKEHGRDRYVVRNFV
>Mature_505_residues
MNLNNFSLRWLTTLNALAVVLGFLMFYLTFKYFWSHDREVAQVLQLQQAELQRVETLLSLERKAMGASLADYAAWDEMAD
FIAKPTLEFTQSNIGEHAFSSQFLDGVFIYDPEGNLVWGKKYDAATGQSSSYEHLLPDFSRILQQATRLSVDEISTSVRY
MVVEDEPYLAATARVCDSDGKGCNKGFLIFIKKVRAQFANVVEQATGVDIEVLTCKNDAPLPQDEVDVSYIKQLDYSGNS
SVLFKINHHIKHPPFIRTEEILALLFFSLVMYLVNLWVVIALIKPITTASQVLQQFKTSGGKMPDASTFISSEMKEFATT
INRIVGQLEDSQQVLRWQSEHDPLTRISNRRHLEKQLKSYLSDRPQAYLVLFLVDIDFFKRFNDSFGHLAGDEALCSVAD
VLQSVEFHGEKIVARFGGEEFCVVLASDCAFDAEQYAQQMRSKIEQLAIANPVDALCQYLTVSIGGVYAISPKMESYLSL
FHQADMALYHAKEHGRDRYVVRNFV

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG3322

COG function: function code T; Predicted periplasmic ligand-binding sensor domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=34.5029239766082, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=32.7586206896552, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082007, Length=184, Percent_Identity=33.1521739130435, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788085, Length=195, Percent_Identity=31.2820512820513, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787802, Length=295, Percent_Identity=30.1694915254237, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693134, Length=192, Percent_Identity=32.8125, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=35.2941176470588, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=30.0578034682081, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788381, Length=186, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=31.2138728323699, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788956, Length=205, Percent_Identity=33.6585365853659, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=33.1325301204819, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 57238; Mature: 57238

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNNFSLRWLTTLNALAVVLGFLMFYLTFKYFWSHDREVAQVLQLQQAELQRVETLLSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ERKAMGASLADYAAWDEMADFIAKPTLEFTQSNIGEHAFSSQFLDGVFIYDPEGNLVWGK
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCEEEECCCCCEEECC
KYDAATGQSSSYEHLLPDFSRILQQATRLSVDEISTSVRYMVVEDEPYLAATARVCDSDG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEEECCCCHHHHHHHHCCCC
KGCNKGFLIFIKKVRAQFANVVEQATGVDIEVLTCKNDAPLPQDEVDVSYIKQLDYSGNS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCC
SVLFKINHHIKHPPFIRTEEILALLFFSLVMYLVNLWVVIALIKPITTASQVLQQFKTSG
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKMPDASTFISSEMKEFATTINRIVGQLEDSQQVLRWQSEHDPLTRISNRRHLEKQLKSY
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LSDRPQAYLVLFLVDIDFFKRFNDSFGHLAGDEALCSVADVLQSVEFHGEKIVARFGGEE
HCCCCCCEEEHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCE
FCVVLASDCAFDAEQYAQQMRSKIEQLAIANPVDALCQYLTVSIGGVYAISPKMESYLSL
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECHHHHHHHHH
FHQADMALYHAKEHGRDRYVVRNFV
HHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MNLNNFSLRWLTTLNALAVVLGFLMFYLTFKYFWSHDREVAQVLQLQQAELQRVETLLSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ERKAMGASLADYAAWDEMADFIAKPTLEFTQSNIGEHAFSSQFLDGVFIYDPEGNLVWGK
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCEEEECCCCCEEECC
KYDAATGQSSSYEHLLPDFSRILQQATRLSVDEISTSVRYMVVEDEPYLAATARVCDSDG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEEECCCCHHHHHHHHCCCC
KGCNKGFLIFIKKVRAQFANVVEQATGVDIEVLTCKNDAPLPQDEVDVSYIKQLDYSGNS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCC
SVLFKINHHIKHPPFIRTEEILALLFFSLVMYLVNLWVVIALIKPITTASQVLQQFKTSG
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKMPDASTFISSEMKEFATTINRIVGQLEDSQQVLRWQSEHDPLTRISNRRHLEKQLKSY
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LSDRPQAYLVLFLVDIDFFKRFNDSFGHLAGDEALCSVADVLQSVEFHGEKIVARFGGEE
HCCCCCCEEEHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCE
FCVVLASDCAFDAEQYAQQMRSKIEQLAIANPVDALCQYLTVSIGGVYAISPKMESYLSL
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECHHHHHHHHH
FHQADMALYHAKEHGRDRYVVRNFV
HHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]