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The map label for this gene is clpB-1 [H]

Identifier: 147675005

GI number: 147675005

Start: 250451

End: 253024

Strand: Direct

Name: clpB-1 [H]

Synonym: VC0395_A0240

Alternate gene names: 147675005

Gene position: 250451-253024 (Clockwise)

Preceding gene: 147674392

Following gene: 147673086

Centisome position: 8.28

GC content: 49.73

Gene sequence:

>2574_bases
ATGCGTCTTGACAGATTTACGAGCAAATTTCAGATCGCGATTTCCGATGCCCAGTCATTAGCGCTCGGCCGTGATCATCA
ATATATCGAACCTGTGCACCTGATGGTGGCTCTGCTGGATCAAAACGGCAGCCCGATCCGCCCATTACTCACCATGCTTA
ATGTGGATGTGATGCAACTGCGCTCCAAACTTGGCGAAATGCTCGATCGTTTACCCAAAGTCAGTGGTATTGGTGGTGAT
GTACAGCTCTCTTCGGCGCTTGGCTCGCTGTTTAATCTGTGTGATAAGGTTGCGCAAAAGCGTCAAGATGCCTACATCTC
CTCTGAAATTTATCTCCTCGCCGCTTTGGAAGATAAAGGTCCACTTGGAACCTTGCTCAAAGAGTTCGGGCTGACCGAGA
AAAAAGTCTCTGATGCAATTGAAAAAATTCGTGGAGGCCAGAAGGTCTATGACCCGAATGCGGAAGAGCTGCGTCAAGCG
TTGGAAAAATTCACCATCGATCTCACTGAACGCGCTGAGCAGGGCAAACTCGATCCTGTGATCGGTCGTGATGATGAAAT
TCGCCGCACCATTCAAGTGCTGCAGCGTCGTACCAAAAACAACCCAGTGATCATCGGTGAACCCGGTGTGGGTAAAACTG
CGATTGTGGAAGGCTTGGCGCAGCGCATCATTAATAATGAAGTACCAGAAGGCCTGCGTGGACGCCGCGTATTGTCTTTA
GATATGGGCGCGCTGGTGGCTGGGGCGAAATACCGCGGCGAATTTGAAGAGCGCTTGAAATCAGTACTCAATGAGCTAGC
GAAAGAAGAAGGCAATATCATCCTCTTCATTGATGAGCTGCACACCATGGTTGGTGCGGGGAAAGGCGAAGGCTCGATGG
ATGCTGGCAACATGCTCAAACCCGCTTTGGCGCGTGGAGAATTGCACTGTGTCGGCGCAACCACTTTAGATGAGTACCGC
CAATACATCGAAAAAGATCCAGCGTTAGAGCGCCGCTTCCAAAAAGTGTTGGTGGATGAGCCCACGGTGGAAGATACCAT
CGCCATCTTGCGTGGATTGAAAGAGCGTTACGAACTGCATCACCATGTCGAAATCACAGATCCAGCGATTGTGGCGGCAG
CGAGCCTATCGCATCGCTATATTTCCGATCGCCAGTTGCCTGATAAAGCGATCGATTTGATCGATGAAGCGGCTTCAAGC
ATCCGGATGCAGATTGACTCCAAACCAGAAGCTCTGGATAAGCTTGAGCGCAAAATCATCCAACTGAAAATCGAGCAGCA
AGCGCTCTCTAATGAGCACGATGAAGCCAGTGAAAAACGCTTGGCGATCTTAAATGAAGAGCTGCAAGAGAAAGAGCGTG
ATTACGCGGAGTTGGAAGAAGTGTGGAAAGCTGAAAAGGCGGCACTCTCCGGCACGCAACATATTAAAGCGGCACTTGAA
CAAGCGCGGATGGATCTGGAAGTCGCTCGCCGTGCGGGGGATTTGAACCGCATGTCAGAGCTGCAATATGGTCGCATTCC
AGAGCTAGAAAAGCAGCTTGATCTGGCTGCGCAAGCCGAAATGCAGGAAATGACTCTGCTACGTAATAAAGTGACGGATG
CAGAAATTGCCGAAGTGCTTTCAAAACAGACTGGCATTCCAGTATCAAAAATGCTCGAAGCCGAGAAAGAAAAGTTGCTT
CGCATGGAAGAAGTGCTGCATAAACGCGTGATTGGTCAAAAAGAAGCGGTGGAAGTGGTCGCGAATGCGATTCGCCGCAG
TCGCGCAGGCCTTTCTGATCCAAATCGTCCGATTGGCTCCTTCTTATTCTTGGGGCCAACAGGGGTCGGTAAAACCGAGC
TGTGTAAAACCTTGGCTAACTTCCTGTTTGACAGTGAAGATGCCATGGTACGTGTGGATATGTCGGAATTTATGGAGAAA
CACTCGGTTGCTCGTTTAGTCGGTGCGCCTCCAGGTTATGTCGGCTATGAAGAAGGTGGCTACTTAACGGAAGCGGTGCG
CCGTAAACCTTACTCGGTGATTTTGCTCGATGAAGTGGAAAAGGCGCACCCAGATGTCTTCAATATTCTGCTGCAAGTCT
TGGATGATGGCCGCTTGACGGACGGGCAAGGGCGCACGGTTGATTTTCGAAATACCGTGGTGATCATGACCTCCAACTTG
GGTTCAAGCCGTATCCAAGAAAATTTTGCAAGGTTGGATTATCAAGGTATCAAAGAACAAGTGATGGACGTAGTCAGCAA
ACACTTCCGCCCTGAGTTCTTGAACCGAGTGGATGAAAGTGTGGTATTCCATCCATTAGGACAAGAGCATATTAAGTCTA
TCGCCTCTATTCAGTTGGCCCGCCTACGTCAACGTTTGGCAGAGCGAGATTACCAACTAGAGGTTGATGATGAGGCGCTG
GATCTGATTGCTCATGTCGGCTTCGATCCGGTTTACGGTGCTCGTCCACTGAAACGCGCGATTCAGCAAAATGTGGAAAA
CCCACTGGCCAAATCTATTCTGGCTGGTAAGTTCCTGCCTGGTTCACCTATTTTACTATCGGTAAAAGACGGGAATATTT
TCGCAAGCCAGTAG

Upstream 100 bases:

>100_bases
CAACAAGAAAGTTTCCTCAACTTCCCTTGAAAATCTGGTTCGTGATAACCATCTTTCCTAACGTATTCAATTTATCTCAG
ATGAGGTTAGGAAGGTTGGT

Downstream 100 bases:

>100_bases
CAGATACCTTATCTATCATAAGTAAAAGAGCCACTTTGAGTGGCTCTTTTGCTTGTTAATTGAACGATGAAGTGCGTTTT
TTAGTGGTTTTGCTGTTAAT

Product: clpB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTMLNVDVMQLRSKLGEMLDRLPKVSGIGGD
VQLSSALGSLFNLCDKVAQKRQDAYISSEIYLLAALEDKGPLGTLLKEFGLTEKKVSDAIEKIRGGQKVYDPNAEELRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGALVAGAKYRGEFEERLKSVLNELAKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASS
IRMQIDSKPEALDKLERKIIQLKIEQQALSNEHDEASEKRLAILNEELQEKERDYAELEEVWKAEKAALSGTQHIKAALE
QARMDLEVARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMTLLRNKVTDAEIAEVLSKQTGIPVSKMLEAEKEKLL
RMEEVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLANFLFDSEDAMVRVDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSSRIQENFARLDYQGIKEQVMDVVSKHFRPEFLNRVDESVVFHPLGQEHIKSIASIQLARLRQRLAERDYQLEVDDEAL
DLIAHVGFDPVYGARPLKRAIQQNVENPLAKSILAGKFLPGSPILLSVKDGNIFASQ

Sequences:

>Translated_857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTMLNVDVMQLRSKLGEMLDRLPKVSGIGGD
VQLSSALGSLFNLCDKVAQKRQDAYISSEIYLLAALEDKGPLGTLLKEFGLTEKKVSDAIEKIRGGQKVYDPNAEELRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGALVAGAKYRGEFEERLKSVLNELAKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASS
IRMQIDSKPEALDKLERKIIQLKIEQQALSNEHDEASEKRLAILNEELQEKERDYAELEEVWKAEKAALSGTQHIKAALE
QARMDLEVARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMTLLRNKVTDAEIAEVLSKQTGIPVSKMLEAEKEKLL
RMEEVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLANFLFDSEDAMVRVDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSSRIQENFARLDYQGIKEQVMDVVSKHFRPEFLNRVDESVVFHPLGQEHIKSIASIQLARLRQRLAERDYQLEVDDEAL
DLIAHVGFDPVYGARPLKRAIQQNVENPLAKSILAGKFLPGSPILLSVKDGNIFASQ
>Mature_857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTMLNVDVMQLRSKLGEMLDRLPKVSGIGGD
VQLSSALGSLFNLCDKVAQKRQDAYISSEIYLLAALEDKGPLGTLLKEFGLTEKKVSDAIEKIRGGQKVYDPNAEELRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGALVAGAKYRGEFEERLKSVLNELAKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASS
IRMQIDSKPEALDKLERKIIQLKIEQQALSNEHDEASEKRLAILNEELQEKERDYAELEEVWKAEKAALSGTQHIKAALE
QARMDLEVARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMTLLRNKVTDAEIAEVLSKQTGIPVSKMLEAEKEKLL
RMEEVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLANFLFDSEDAMVRVDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSSRIQENFARLDYQGIKEQVMDVVSKHFRPEFLNRVDESVVFHPLGQEHIKSIASIQLARLRQRLAERDYQLEVDDEAL
DLIAHVGFDPVYGARPLKRAIQQNVENPLAKSILAGKFLPGSPILLSVKDGNIFASQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=322, Percent_Identity=38.1987577639752, Blast_Score=212, Evalue=1e-54,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=76.3127187864644, Blast_Score=1344, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=333, Percent_Identity=41.1411411411411, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6320464, Length=709, Percent_Identity=52.1861777150917, Blast_Score=754, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=864, Percent_Identity=41.6666666666667, Blast_Score=654, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95842; Mature: 95842

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTMLNVDVMQL
CCCHHHCCHHEEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RSKLGEMLDRLPKVSGIGGDVQLSSALGSLFNLCDKVAQKRQDAYISSEIYLLAALEDKG
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PLGTLLKEFGLTEKKVSDAIEKIRGGQKVYDPNAEELRQALEKFTIDLTERAEQGKLDPV
CHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKSVLNELAKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLK
CHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRQYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASSIRMQIDSKPEALDKLERKII
HCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
QLKIEQQALSNEHDEASEKRLAILNEELQEKERDYAELEEVWKAEKAALSGTQHIKAALE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QARMDLEVARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMTLLRNKVTDAEIAEVL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SKQTGIPVSKMLEAEKEKLLRMEEVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLANFLFDSEDAMVRVDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSSRIQENFARLDYQGIKEQVMDVVSKHFRPEFLNRVDESVVFHPLGQEHIKSIASIQLA
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
RLRQRLAERDYQLEVDDEALDLIAHVGFDPVYGARPLKRAIQQNVENPLAKSILAGKFLP
HHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCC
GSPILLSVKDGNIFASQ
CCCEEEEEECCCEECCC
>Mature Secondary Structure
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTMLNVDVMQL
CCCHHHCCHHEEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RSKLGEMLDRLPKVSGIGGDVQLSSALGSLFNLCDKVAQKRQDAYISSEIYLLAALEDKG
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PLGTLLKEFGLTEKKVSDAIEKIRGGQKVYDPNAEELRQALEKFTIDLTERAEQGKLDPV
CHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKSVLNELAKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLK
CHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRQYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASLSHRYISDRQLPDKAIDLIDEAASSIRMQIDSKPEALDKLERKII
HCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
QLKIEQQALSNEHDEASEKRLAILNEELQEKERDYAELEEVWKAEKAALSGTQHIKAALE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QARMDLEVARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMTLLRNKVTDAEIAEVL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SKQTGIPVSKMLEAEKEKLLRMEEVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLANFLFDSEDAMVRVDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSSRIQENFARLDYQGIKEQVMDVVSKHFRPEFLNRVDESVVFHPLGQEHIKSIASIQLA
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
RLRQRLAERDYQLEVDDEALDLIAHVGFDPVYGARPLKRAIQQNVENPLAKSILAGKFLP
HHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCC
GSPILLSVKDGNIFASQ
CCCEEEEEECCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]