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The map label for this gene is prfC

Identifier: 147674859

GI number: 147674859

Start: 194665

End: 196260

Strand: Direct

Name: prfC

Synonym: VC0395_A0190

Alternate gene names: 147674859

Gene position: 194665-196260 (Clockwise)

Preceding gene: 147674031

Following gene: 147673070

Centisome position: 6.44

GC content: 50.19

Gene sequence:

>1596_bases
TTGTTGATGTCAACTACACCCTATTTCGGCGAAGTATCAAAGCGCCGTACTTTCGCGATCATCTCGCACCCTGACGCGGG
TAAAACCACGATTACTGAAAAAGTCCTGCTTTTCGGACGTGCGATCCAAGTAGCCGGCACGGTAAAAGGCCGTGGCTCAA
ACCAGCATGCAAAATCTGACTGGATGGAAATGGAAAAAGAGCGTGGTATTTCGGTGACCACCTCTGTTATGCAATTCCCG
TACGGCGATTGTCTGGTGAACCTTTTGGATACTCCGGGACACGAAGACTTCTCGGAAGATACTTATCGTACCCTGACCGC
AGTTGACTCTTGTTTGATGGTGATTGATGCGGCGAAAGGGGTTGAAGATCGTACCCGTAAGTTGATGGAAGTGACCCGTC
TGCGTGATACGCCGATCGTGACCTTCATGAACAAACTTGACCGTGAAATCCGTGACCCGATGGAGTTGATGGATGAAGTG
GAAAATGAGCTGAAAATTGCTTGTTCGCCGATCACATGGCCAATCGGTTGTGGTAAAGAGTTTAAAGGTGTTTACCACAT
TCATCGTGATGAAACCATTTTGTACACTTCAGGCCAAGGCCACACCATCCAAGAAGAACGCATTATCAAAGGCTTGGATA
ACCCTGAGCTGGATCAAGCCGTCGGTGCTAATTTAGCGGCGCAGCTGCGTGAAGAGTTAGAATTGGTACTCGGCGCGTCA
CATGAGTTTGATCGTGAGTTGTTCCTGCAAGGCGAACTGACTCCGGTATTCTTTGGTACAGCACTGGGTAACTTTGGGGT
TGATCATATGCTGGACGGCCTGACCCAATGGGCACCATCACCTATGCCTCGTCAGGCGGCTGAGCGTGTGGTTGAGGCGA
GCGAAGAGAAATTCACTGGCTTTGTGTTTAAAATTCAAGCCAACATGGATCCTAAACATCGCGACCGGATTGCTTTCGTG
CGTATTGTATCGGGCACCTACAAGCAAGGCATGAAGATGAACCATGTGCGCCTTGGTAAGCAGGTTAATATTTCTGATGC
GGTAACCTTTATGGCGGGCGACCGTGCACGCGCAGAAGAGGCATTTGCTGGCGATATTATCGGCCTGCATAACCACGGCA
CGATCCAGATTGGCGATACTTTTACCCAAGGTGAAACGCTGAAATTTACTGGTATTCCGAACTTCGCGCCTGAACTGTTC
CGCCGTATTCGTCTGCGTGACCCACTCAAGCAAAAGCAACTGCTGAAAGGCTTAGTACAGCTTTCGGAAGAGGGTGCGGT
GCAGGTTTTCCGTCCGCTCCAAAACAACGACCTCATCGTGGGTGCGGTGGGGGTACTGCAGTTTGATGTGGTGGTATCGC
GTCTGAAATCGGAATACAACGTTGAAGCCATCTATGAAGGCGTTAACGTTGCGACCGCGCGTTGGGTTGAGTGTGACGAT
GTGAAGAAGTTTGAAGAGTTCAAACGCAAAAACCAGAGCAACCTTGCGCTGGATGGGGGCGATAATCTTGCGTATATCGC
ACCCACCATGGTGAATTTGAACTTGGCGCAGGAGCGTTCACCTGAGGTGAAATTCCGCGCGACTCGTGAACACTAA

Upstream 100 bases:

>100_bases
AGTGAAGTTGCTCCCTGCGCCAAGATAGGGGAGAATACCGCGCAAATTTGAACCGAAACGCTCGCTAGCTTATGTTGGGC
TGATTGAGCACAAAGAGAAC

Downstream 100 bases:

>100_bases
GGATTAAGCTTAACGTGCGCTGAATATGGTGCGCGTTGAGCTAAGCCAATAAAAAAGCCGATGAAAATGCATCGGCTTTT
TCGTAACTGGATTACCTATC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MLMSTTPYFGEVSKRRTFAIISHPDAGKTTITEKVLLFGRAIQVAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFP
YGDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDREIRDPMELMDEV
ENELKIACSPITWPIGCGKEFKGVYHIHRDETILYTSGQGHTIQEERIIKGLDNPELDQAVGANLAAQLREELELVLGAS
HEFDRELFLQGELTPVFFGTALGNFGVDHMLDGLTQWAPSPMPRQAAERVVEASEEKFTGFVFKIQANMDPKHRDRIAFV
RIVSGTYKQGMKMNHVRLGKQVNISDAVTFMAGDRARAEEAFAGDIIGLHNHGTIQIGDTFTQGETLKFTGIPNFAPELF
RRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYEGVNVATARWVECDD
VKKFEEFKRKNQSNLALDGGDNLAYIAPTMVNLNLAQERSPEVKFRATREH

Sequences:

>Translated_531_residues
MLMSTTPYFGEVSKRRTFAIISHPDAGKTTITEKVLLFGRAIQVAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFP
YGDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDREIRDPMELMDEV
ENELKIACSPITWPIGCGKEFKGVYHIHRDETILYTSGQGHTIQEERIIKGLDNPELDQAVGANLAAQLREELELVLGAS
HEFDRELFLQGELTPVFFGTALGNFGVDHMLDGLTQWAPSPMPRQAAERVVEASEEKFTGFVFKIQANMDPKHRDRIAFV
RIVSGTYKQGMKMNHVRLGKQVNISDAVTFMAGDRARAEEAFAGDIIGLHNHGTIQIGDTFTQGETLKFTGIPNFAPELF
RRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYEGVNVATARWVECDD
VKKFEEFKRKNQSNLALDGGDNLAYIAPTMVNLNLAQERSPEVKFRATREH
>Mature_531_residues
MLMSTTPYFGEVSKRRTFAIISHPDAGKTTITEKVLLFGRAIQVAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFP
YGDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKLDREIRDPMELMDEV
ENELKIACSPITWPIGCGKEFKGVYHIHRDETILYTSGQGHTIQEERIIKGLDNPELDQAVGANLAAQLREELELVLGAS
HEFDRELFLQGELTPVFFGTALGNFGVDHMLDGLTQWAPSPMPRQAAERVVEASEEKFTGFVFKIQANMDPKHRDRIAFV
RIVSGTYKQGMKMNHVRLGKQVNISDAVTFMAGDRARAEEAFAGDIIGLHNHGTIQIGDTFTQGETLKFTGIPNFAPELF
RRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYEGVNVATARWVECDD
VKKFEEFKRKNQSNLALDGGDNLAYIAPTMVNLNLAQERSPEVKFRATREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=491, Percent_Identity=26.2729124236253, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI19923640, Length=512, Percent_Identity=26.3671875, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI25306283, Length=396, Percent_Identity=28.5353535353535, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI25306287, Length=484, Percent_Identity=25.2066115702479, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI157426893, Length=173, Percent_Identity=30.0578034682081, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI94966754, Length=158, Percent_Identity=29.746835443038, Blast_Score=77, Evalue=6e-14,
Organism=Escherichia coli, GI1790835, Length=529, Percent_Identity=83.7429111531191, Blast_Score=946, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=505, Percent_Identity=27.7227722772277, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI48994988, Length=176, Percent_Identity=31.8181818181818, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=549, Percent_Identity=24.9544626593807, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=248, Percent_Identity=30.6451612903226, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=31.5789473684211, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=527, Percent_Identity=25.6166982922201, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6322359, Length=421, Percent_Identity=26.1282660332542, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=24.8407643312102, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324707, Length=157, Percent_Identity=26.7515923566879, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=157, Percent_Identity=26.7515923566879, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=486, Percent_Identity=25.9259259259259, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI221458488, Length=521, Percent_Identity=24.7600767754319, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI78706572, Length=214, Percent_Identity=30.3738317757009, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585711, Length=155, Percent_Identity=26.4516129032258, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24585713, Length=155, Percent_Identity=26.4516129032258, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24585709, Length=155, Percent_Identity=26.4516129032258, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_VIBC3 (A5F904)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F904
- SMR:   A5F904
- STRING:   A5F904
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0190
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- BioCyc:   VCHO345073:VC0395_A0190-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59626; Mature: 59626

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMSTTPYFGEVSKRRTFAIISHPDAGKTTITEKVLLFGRAIQVAGTVKGRGSNQHAKSD
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHH
WMEMEKERGISVTTSVMQFPYGDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKG
HHHHHHHCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
VEDRTRKLMEVTRLRDTPIVTFMNKLDREIRDPMELMDEVENELKIACSPITWPIGCGKE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCCCCCCCCC
FKGVYHIHRDETILYTSGQGHTIQEERIIKGLDNPELDQAVGANLAAQLREELELVLGAS
CCCEEEEECCCEEEEECCCCCEECHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHCCC
HEFDRELFLQGELTPVFFGTALGNFGVDHMLDGLTQWAPSPMPRQAAERVVEASEEKFTG
CCCCCEEEEECCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEE
FVFKIQANMDPKHRDRIAFVRIVSGTYKQGMKMNHVRLGKQVNISDAVTFMAGDRARAEE
EEEEEEECCCCCHHCCEEEEEEECCHHHCCCEECCEECCCEECHHHHEEEEECCHHHHHH
AFAGDIIGLHNHGTIQIGDTFTQGETLKFTGIPNFAPELFRRIRLRDPLKQKQLLKGLVQ
HHCCCEEEECCCCEEEECCEECCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYEGVNVATARWVECDD
HCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCHHH
VKKFEEFKRKNQSNLALDGGDNLAYIAPTMVNLNLAQERSPEVKFRATREH
HHHHHHHHHCCCCCEEEECCCCEEEECCEEEEEEEHHCCCCCEEEEECCCC
>Mature Secondary Structure
MLMSTTPYFGEVSKRRTFAIISHPDAGKTTITEKVLLFGRAIQVAGTVKGRGSNQHAKSD
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHH
WMEMEKERGISVTTSVMQFPYGDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKG
HHHHHHHCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
VEDRTRKLMEVTRLRDTPIVTFMNKLDREIRDPMELMDEVENELKIACSPITWPIGCGKE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCCCCCCCCC
FKGVYHIHRDETILYTSGQGHTIQEERIIKGLDNPELDQAVGANLAAQLREELELVLGAS
CCCEEEEECCCEEEEECCCCCEECHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHCCC
HEFDRELFLQGELTPVFFGTALGNFGVDHMLDGLTQWAPSPMPRQAAERVVEASEEKFTG
CCCCCEEEEECCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEE
FVFKIQANMDPKHRDRIAFVRIVSGTYKQGMKMNHVRLGKQVNISDAVTFMAGDRARAEE
EEEEEEECCCCCHHCCEEEEEEECCHHHCCCEECCEECCCEECHHHHEEEEECCHHHHHH
AFAGDIIGLHNHGTIQIGDTFTQGETLKFTGIPNFAPELFRRIRLRDPLKQKQLLKGLVQ
HHCCCEEEECCCCEEEECCEECCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYEGVNVATARWVECDD
HCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCHHH
VKKFEEFKRKNQSNLALDGGDNLAYIAPTMVNLNLAQERSPEVKFRATREH
HHHHHHHHHCCCCCEEEECCCCEEEECCEEEEEEEHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA