Click here to switch to the map view.

The map label for this gene is lspA

Identifier: 147674341

GI number: 147674341

Start: 222329

End: 222844

Strand: Direct

Name: lspA

Synonym: VC0395_A0215

Alternate gene names: 147674341

Gene position: 222329-222844 (Clockwise)

Preceding gene: 147675239

Following gene: 147674041

Centisome position: 7.35

GC content: 49.42

Gene sequence:

>516_bases
ATGTCGAATTCGTCTCTGACTCTTAAACAATCTGGATTGCGCTGGCTGTGGCTGGCGCTTCTGGTTTTCATTGCTGACAT
CACGATCAAGTTAATTGTGATGGATAACATGGGCTATGGTTGGGCAAACCGTATTGAGGTGCTGCCTTTCTTTAACCTGC
TGTATGTGCATAACTATGGCGCGGCTTTTAGCTTTCTGAGCGATCAGGAAGGGTGGCAGCGCTGGCTATTTACGGGTATT
GCCTTTGTGGTCACCGGCATGCTCGCTTACTGGATGCGTCGTCTTCCGGCGAGTGATAAGTGGAACAATATTGCCTACGC
ACTGATCATCGGTGGTGCCGTGGGCAATGTGTTCGATCGCATCGTGCATGGTTTTGTGGTCGATTATCTCGATTTTTACT
GGGGAACCTACCACTGGCCAGCATTCAATCTGGCCGACAGTACGATCTGTATCGGTGCAGCGATGATTATTCTCGATGGT
TTTCGCGCTAAGAAAAGTGCGCCAAGTCAGTCTTAG

Upstream 100 bases:

>100_bases
AGGCACGATTGCAGGTCACACCACCATTTGTGGTCGTTGCGTGTCTAACGTTGAGGGTGAAGGCGAAGTACGCAAATTTG
CGTAATTCAGGCTAACCATC

Downstream 100 bases:

>100_bases
TGCTCTGCATTGCGCCGTAAAGCAAAGACACTGCGGCAAATAACCACAAGCGCCGTCCGTTGATTCGGAGGGCGCTTTTT
ACTATAAAGGCTGGCATCAG

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MSNSSLTLKQSGLRWLWLALLVFIADITIKLIVMDNMGYGWANRIEVLPFFNLLYVHNYGAAFSFLSDQEGWQRWLFTGI
AFVVTGMLAYWMRRLPASDKWNNIAYALIIGGAVGNVFDRIVHGFVVDYLDFYWGTYHWPAFNLADSTICIGAAMIILDG
FRAKKSAPSQS

Sequences:

>Translated_171_residues
MSNSSLTLKQSGLRWLWLALLVFIADITIKLIVMDNMGYGWANRIEVLPFFNLLYVHNYGAAFSFLSDQEGWQRWLFTGI
AFVVTGMLAYWMRRLPASDKWNNIAYALIIGGAVGNVFDRIVHGFVVDYLDFYWGTYHWPAFNLADSTICIGAAMIILDG
FRAKKSAPSQS
>Mature_170_residues
SNSSLTLKQSGLRWLWLALLVFIADITIKLIVMDNMGYGWANRIEVLPFFNLLYVHNYGAAFSFLSDQEGWQRWLFTGIA
FVVTGMLAYWMRRLPASDKWNNIAYALIIGGAVGNVFDRIVHGFVVDYLDFYWGTYHWPAFNLADSTICIGAAMIILDGF
RAKKSAPSQS

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=165, Percent_Identity=53.9393939393939, Blast_Score=176, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_VIBC3 (A5F8Z4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- STRING:   A5F8Z4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0215
- eggNOG:   COG0597
- HOGENOM:   HBG724422
- OMA:   ADSSGWQ
- ProtClustDB:   PRK00376
- BioCyc:   VCHO345073:VC0395_A0215-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 19420; Mature: 19289

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 119-119 ACT_SITE 146-146

Signals:

None

Transmembrane regions:

HASH(0x1815d9f0)-; HASH(0x17f1987c)-; HASH(0x17c618dc)-; HASH(0x17175568)-; HASH(0x16f18718)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSSLTLKQSGLRWLWLALLVFIADITIKLIVMDNMGYGWANRIEVLPFFNLLYVHNYG
CCCCCEEHHHCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
AAFSFLSDQEGWQRWLFTGIAFVVTGMLAYWMRRLPASDKWNNIAYALIIGGAVGNVFDR
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHH
IVHGFVVDYLDFYWGTYHWPAFNLADSTICIGAAMIILDGFRAKKSAPSQS
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHCCCCCCCH
>Mature Secondary Structure 
SNSSLTLKQSGLRWLWLALLVFIADITIKLIVMDNMGYGWANRIEVLPFFNLLYVHNYG
CCCCEEHHHCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
AAFSFLSDQEGWQRWLFTGIAFVVTGMLAYWMRRLPASDKWNNIAYALIIGGAVGNVFDR
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHH
IVHGFVVDYLDFYWGTYHWPAFNLADSTICIGAAMIILDGFRAKKSAPSQS
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA