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The map label for this gene is pstA-1 [H]

Identifier: 147674308

GI number: 147674308

Start: 272023

End: 273669

Strand: Direct

Name: pstA-1 [H]

Synonym: VC0395_A0259

Alternate gene names: 147674308

Gene position: 272023-273669 (Clockwise)

Preceding gene: 147675548

Following gene: 147673468

Centisome position: 9.0

GC content: 49.42

Gene sequence:

>1647_bases
ATGTTGAATTGGTTTAAATCCGGTGCTCCGTGGATCTGGCTGACAGGAGGAGCCGTCAGCGTCAGCTTGATTGCTGTACT
TGGCCTATTACTGGTGATTGGTTGGCGTGGCTTAACCTATTTCTGGCCAGCGCCCTTGCTGCAATGGCAAACCCCCGATG
GAAAAAACGTGGTGGGTCAACTGTACGCTCGTGAAAGTGTGCCGGTTAGCCATTTAAAAGAGATGAATTTACCGCTACCA
GAAAAAGTGCAAGCGGCAGGGTTGGCGACACGGCTCAATATCAAAGTCGCTAATCGGGATCTGTATGGATCTGATTTTAT
TGCTTTACTTGAGTTTGAAACCGAATTTAAAGGCGCGCCACAAGGTTGGGCGGTGATTGAGCGCAGCCGAGGTGGGCAAC
TGTTTGGTAAACCGCTTCGCTTCGATTCTACGATTGGCGAGCTGGATCTGCCGCTAGAGCGCCATCTGCGTCAAGCATTG
TTAGAGGCTGAAGCCATTCGCGATCAAGTGGATACGATGGTTGAACAGCAAGTGCGGGTGATCAGTAGCCAGCTAGAAAA
ATTACGTTTGGAAAAACGCCGTAAGCAATTGAATAACCAGCTCAGTGATGAGTTTTTACAACACTACGTGTTAAGCAAAA
GCCGTTTAGAGAAAGAGCTTGCACTGGTTCAAACTGAGTTAGATGCGCTCCGAGCACCGTTAGAAAAGCAGTTTTTATAT
CTTGTGGATATGCAAGGAGAGACCGTCTCCGTCCCGCTGAGTGAAATCTTGGATTTTTGGTATCCCAATGATATGAATTT
TTCCCAAAAACTCGCGGAGTGGGGCAAGCAAGCATGGCGCTTTTTATCGGAATCACCACGGGAGTCAAACTCGGAAGGGG
GCGTGTTTCCGGCCATTTTTGGAACGGTGTTTCTGGTGATCATCATGTCGATCATCGTCATGCCTTTAGGCGTGATAGCA
GCTATCTATCTGCATGAATACGCGCAAGAGGGCGCATTTACTCGGATGATTCGAGTCGCGGTGATCAACTTAGCGGGGGT
ACCTTCCATCGTATATGGGGTGTTTGGCCTCGGCTTTTTTGTTTACACCATAGGCGCTTCGGTTGATAACCTTTTTTATG
CTGAGCGTTTGCCCACTCCGACCTTCGGGACGCCTGGGTTGCTGTGGTCTGCTCTGACATTAGCCGTGTTGACGCTGCCT
GTGGTGATTGTGGCGACGGAAGAAGGGCTCTCGCGTATTCCGATTTCGGTGCGCCATGGATCATTAGCACTAGGCGCTAC
CCAGTTTGAAACCATTTGGCGTATTGTGCTACCGATGGCAAGCCCTGCGATCATCACCGGATTGATTTTGGCGATCGCCC
GCGCAGCCGGTGAAGTGGCTCCGCTGATGCTGGTCGGTGCGGTGAAACTGGCTTCCAGTTTGCCTGTGGATGGGGAATTT
CCCTTCATCCATCTTGAACGCAAATTTATGCATTTAGGTTTCCACATTTACGATATCGGTTTTCAAACCAACAATATTGA
AGCGGCACGGCCACTGGTGTATGCCACTTCATTTTTATTGGTCACTGTGATTGTGGCGCTCAACTTAACTGCGATCAGCA
TTCGTAATAACTTACGCGAAAAATACCGAACCTTGGGGCAAGACTAG

Upstream 100 bases:

>100_bases
CGCACTGCTGCTGCTGACCTTTACGTTTGCGGTGAATTCCCTAGCGGAATGGGTACGCCAACGGCTACGAGAAAAATACC
GTTCTTTATGATGGAGCAAT

Downstream 100 bases:

>100_bases
TTATGTACTCCGCCAATCCTACTTTGGGCTATTATGTGCCGCCGCTGGACGTGAATAATCTTACCGATCAGCAGACGGCG
ATTTCGATTGAGCACTTAAG

Product: phosphate ABC transporter, permease protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MLNWFKSGAPWIWLTGGAVSVSLIAVLGLLLVIGWRGLTYFWPAPLLQWQTPDGKNVVGQLYARESVPVSHLKEMNLPLP
EKVQAAGLATRLNIKVANRDLYGSDFIALLEFETEFKGAPQGWAVIERSRGGQLFGKPLRFDSTIGELDLPLERHLRQAL
LEAEAIRDQVDTMVEQQVRVISSQLEKLRLEKRRKQLNNQLSDEFLQHYVLSKSRLEKELALVQTELDALRAPLEKQFLY
LVDMQGETVSVPLSEILDFWYPNDMNFSQKLAEWGKQAWRFLSESPRESNSEGGVFPAIFGTVFLVIIMSIIVMPLGVIA
AIYLHEYAQEGAFTRMIRVAVINLAGVPSIVYGVFGLGFFVYTIGASVDNLFYAERLPTPTFGTPGLLWSALTLAVLTLP
VVIVATEEGLSRIPISVRHGSLALGATQFETIWRIVLPMASPAIITGLILAIARAAGEVAPLMLVGAVKLASSLPVDGEF
PFIHLERKFMHLGFHIYDIGFQTNNIEAARPLVYATSFLLVTVIVALNLTAISIRNNLREKYRTLGQD

Sequences:

>Translated_548_residues
MLNWFKSGAPWIWLTGGAVSVSLIAVLGLLLVIGWRGLTYFWPAPLLQWQTPDGKNVVGQLYARESVPVSHLKEMNLPLP
EKVQAAGLATRLNIKVANRDLYGSDFIALLEFETEFKGAPQGWAVIERSRGGQLFGKPLRFDSTIGELDLPLERHLRQAL
LEAEAIRDQVDTMVEQQVRVISSQLEKLRLEKRRKQLNNQLSDEFLQHYVLSKSRLEKELALVQTELDALRAPLEKQFLY
LVDMQGETVSVPLSEILDFWYPNDMNFSQKLAEWGKQAWRFLSESPRESNSEGGVFPAIFGTVFLVIIMSIIVMPLGVIA
AIYLHEYAQEGAFTRMIRVAVINLAGVPSIVYGVFGLGFFVYTIGASVDNLFYAERLPTPTFGTPGLLWSALTLAVLTLP
VVIVATEEGLSRIPISVRHGSLALGATQFETIWRIVLPMASPAIITGLILAIARAAGEVAPLMLVGAVKLASSLPVDGEF
PFIHLERKFMHLGFHIYDIGFQTNNIEAARPLVYATSFLLVTVIVALNLTAISIRNNLREKYRTLGQD
>Mature_548_residues
MLNWFKSGAPWIWLTGGAVSVSLIAVLGLLLVIGWRGLTYFWPAPLLQWQTPDGKNVVGQLYARESVPVSHLKEMNLPLP
EKVQAAGLATRLNIKVANRDLYGSDFIALLEFETEFKGAPQGWAVIERSRGGQLFGKPLRFDSTIGELDLPLERHLRQAL
LEAEAIRDQVDTMVEQQVRVISSQLEKLRLEKRRKQLNNQLSDEFLQHYVLSKSRLEKELALVQTELDALRAPLEKQFLY
LVDMQGETVSVPLSEILDFWYPNDMNFSQKLAEWGKQAWRFLSESPRESNSEGGVFPAIFGTVFLVIIMSIIVMPLGVIA
AIYLHEYAQEGAFTRMIRVAVINLAGVPSIVYGVFGLGFFVYTIGASVDNLFYAERLPTPTFGTPGLLWSALTLAVLTLP
VVIVATEEGLSRIPISVRHGSLALGATQFETIWRIVLPMASPAIITGLILAIARAAGEVAPLMLVGAVKLASSLPVDGEF
PFIHLERKFMHLGFHIYDIGFQTNNIEAARPLVYATSFLLVTVIVALNLTAISIRNNLREKYRTLGQD

Specific function: Part of the binding-protein-dependent transport system yqgGHIJK. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4985

COG function: function code P; ABC-type phosphate transport system, auxiliary component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790163, Length=183, Percent_Identity=34.9726775956284, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790164, Length=178, Percent_Identity=31.4606741573034, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005672 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 60970; Mature: 60970

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNWFKSGAPWIWLTGGAVSVSLIAVLGLLLVIGWRGLTYFWPAPLLQWQTPDGKNVVGQ
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHH
LYARESVPVSHLKEMNLPLPEKVQAAGLATRLNIKVANRDLYGSDFIALLEFETEFKGAP
HHHCCCCCHHHHHHCCCCCCHHHHHCCCEEEEEEEEECCCCCCCCEEEEEEECHHCCCCC
QGWAVIERSRGGQLFGKPLRFDSTIGELDLPLERHLRQALLEAEAIRDQVDTMVEQQVRV
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISSQLEKLRLEKRRKQLNNQLSDEFLQHYVLSKSRLEKELALVQTELDALRAPLEKQFLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEE
LVDMQGETVSVPLSEILDFWYPNDMNFSQKLAEWGKQAWRFLSESPRESNSEGGVFPAIF
EEECCCCEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
GTVFLVIIMSIIVMPLGVIAAIYLHEYAQEGAFTRMIRVAVINLAGVPSIVYGVFGLGFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VYTIGASVDNLFYAERLPTPTFGTPGLLWSALTLAVLTLPVVIVATEEGLSRIPISVRHG
HHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCEEEECC
SLALGATQFETIWRIVLPMASPAIITGLILAIARAAGEVAPLMLVGAVKLASSLPVDGEF
CEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
PFIHLERKFMHLGFHIYDIGFQTNNIEAARPLVYATSFLLVTVIVALNLTAISIRNNLRE
CEEEHHHHHHHCCEEEEEECEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYRTLGQD
HHHHCCCC
>Mature Secondary Structure
MLNWFKSGAPWIWLTGGAVSVSLIAVLGLLLVIGWRGLTYFWPAPLLQWQTPDGKNVVGQ
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHH
LYARESVPVSHLKEMNLPLPEKVQAAGLATRLNIKVANRDLYGSDFIALLEFETEFKGAP
HHHCCCCCHHHHHHCCCCCCHHHHHCCCEEEEEEEEECCCCCCCCEEEEEEECHHCCCCC
QGWAVIERSRGGQLFGKPLRFDSTIGELDLPLERHLRQALLEAEAIRDQVDTMVEQQVRV
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISSQLEKLRLEKRRKQLNNQLSDEFLQHYVLSKSRLEKELALVQTELDALRAPLEKQFLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEE
LVDMQGETVSVPLSEILDFWYPNDMNFSQKLAEWGKQAWRFLSESPRESNSEGGVFPAIF
EEECCCCEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
GTVFLVIIMSIIVMPLGVIAAIYLHEYAQEGAFTRMIRVAVINLAGVPSIVYGVFGLGFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VYTIGASVDNLFYAERLPTPTFGTPGLLWSALTLAVLTLPVVIVATEEGLSRIPISVRHG
HHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCEEEECC
SLALGATQFETIWRIVLPMASPAIITGLILAIARAAGEVAPLMLVGAVKLASSLPVDGEF
CEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
PFIHLERKFMHLGFHIYDIGFQTNNIEAARPLVYATSFLLVTVIVALNLTAISIRNNLRE
CEEEHHHHHHHCCEEEEEECEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYRTLGQD
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]