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The map label for this gene is thyA

Identifier: 147673512

GI number: 147673512

Start: 212346

End: 213197

Strand: Direct

Name: thyA

Synonym: VC0395_A0207

Alternate gene names: 147673512

Gene position: 212346-213197 (Clockwise)

Preceding gene: 147674969

Following gene: 147674750

Centisome position: 7.02

GC content: 45.89

Gene sequence:

>852_bases
GTGAAACAGTATTTAGATCTTTGTCAGCGCATCGTCGATCAAGGTGTTTGGGTTGAAAATGAACGAACGGGCAAGCGTTG
TTTGACTGTGATTAATGCCGATTTGACCTACGATGTGGGCAACAATCAGTTTCCTCTAGTGACTACACGCAAGAGTTTTT
GGAAAGCTGCCGTAGCCGAGTTGCTCGGCTATATTCGTGGTTACGATAATGCGGCGGATTTTCGCCAATTAGGTACCAAA
ACCTGGGATGCTAATGCCAATTTAAACCAAGCATGGCTCAACAATCCTTACCGTAAAGGTGAGGATGACATGGGACGCGT
GTATGGTGTTCAGGGTAGAGCTTGGGCTAAGCCTGATGGTGGTCATATTGACCAGTTGAAAAAGATTGTTGATGATTTGA
GCCGTGGCGTTGATGACCGAGGTGAAATTCTTAACTTCTACAATCCGGGTGAATTTCACATGGGGTGTTTGCGCCCTTGC
ATGTACAGCCATCATTTTTCATTGCTGGGGGATACCTTGTATCTCAACAGTACTCAGCGTTCATGTGATGTGCCCTTGGG
GTTGAATTTCAACATGGTGCAGGTTTATGTGTTCCTTGCGCTGATGGCACAGATCACAGGGAAAAAGCCGGGCTTGGCGT
ATCACAAGATCGTCAATGCGCACATTTACCAAGATCAACTCGAATTGATGCGCGATGTGCAGCTAAAACGTGAGCCATTC
CCAGCGCCTCAGTTCCATATCAATCCAAAGATTAAAACACTGCAGGATTTGGAAACTTGGGTCACTTTGGATGATTTTGA
CGTCACCGGATATCAGTTCCACGATCCTATTCAATACCCGTTTTCAGTCTAA

Upstream 100 bases:

>100_bases
CCTCTCCTTACCTATGGTGATCATCGGTATTTTGATGATGGTTTGGTCTTACAAGCGCGGTTTGTATCAAGACCGTGTAG
CAGCAAAATAGGGTAGTTAG

Downstream 100 bases:

>100_bases
TCCCGTATTCAGGCGGTATGGCTTGATGGGTTTTATATAAAAAAAGCTCCCGAAGGTCGGGAGCTTTTTTTATACAGATG
ATGCTTTAACGCTTAAGCGG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKQYLDLCQRIVDQGVWVENERTGKRCLTVINADLTYDVGNNQFPLVTTRKSFWKAAVAELLGYIRGYDNAADFRQLGTK
TWDANANLNQAWLNNPYRKGEDDMGRVYGVQGRAWAKPDGGHIDQLKKIVDDLSRGVDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLALMAQITGKKPGLAYHKIVNAHIYQDQLELMRDVQLKREPF
PAPQFHINPKIKTLQDLETWVTLDDFDVTGYQFHDPIQYPFSV

Sequences:

>Translated_283_residues
MKQYLDLCQRIVDQGVWVENERTGKRCLTVINADLTYDVGNNQFPLVTTRKSFWKAAVAELLGYIRGYDNAADFRQLGTK
TWDANANLNQAWLNNPYRKGEDDMGRVYGVQGRAWAKPDGGHIDQLKKIVDDLSRGVDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLALMAQITGKKPGLAYHKIVNAHIYQDQLELMRDVQLKREPF
PAPQFHINPKIKTLQDLETWVTLDDFDVTGYQFHDPIQYPFSV
>Mature_283_residues
MKQYLDLCQRIVDQGVWVENERTGKRCLTVINADLTYDVGNNQFPLVTTRKSFWKAAVAELLGYIRGYDNAADFRQLGTK
TWDANANLNQAWLNNPYRKGEDDMGRVYGVQGRAWAKPDGGHIDQLKKIVDDLSRGVDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLALMAQITGKKPGLAYHKIVNAHIYQDQLELMRDVQLKREPF
PAPQFHINPKIKTLQDLETWVTLDDFDVTGYQFHDPIQYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=33.2179930795848, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=32.5088339222615, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71993377, Length=275, Percent_Identity=27.2727272727273, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324648, Length=303, Percent_Identity=26.7326732673267, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137556, Length=293, Percent_Identity=29.0102389078498, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_VIBC3 (A5F8Y6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F8Y6
- SMR:   A5F8Y6
- STRING:   A5F8Y6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0207
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   GRAWRKP
- ProtClustDB:   PRK01827
- BioCyc:   VCHO345073:VC0395_A0207-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32545; Mature: 32545

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLCQRIVDQGVWVENERTGKRCLTVINADLTYDVGNNQFPLVTTRKSFWKAAVAE
CCHHHHHHHHHHHCCCEECCCCCCCCEEEEEECCEEEECCCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAADFRQLGTKTWDANANLNQAWLNNPYRKGEDDMGRVYGVQGRAWAKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHEEECCCCEECCCCC
GHIDQLKKIVDDLSRGVDDRGEILNFYNPGEFHMGCLRPCMYSHHFSLLGDTLYLNSTQR
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
SCDVPLGLNFNMVQVYVFLALMAQITGKKPGLAYHKIVNAHIYQDQLELMRDVQLKREPF
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PAPQFHINPKIKTLQDLETWVTLDDFDVTGYQFHDPIQYPFSV
CCCCEEECCCHHHHHHHHHHEEEECCCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MKQYLDLCQRIVDQGVWVENERTGKRCLTVINADLTYDVGNNQFPLVTTRKSFWKAAVAE
CCHHHHHHHHHHHCCCEECCCCCCCCEEEEEECCEEEECCCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAADFRQLGTKTWDANANLNQAWLNNPYRKGEDDMGRVYGVQGRAWAKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHEEECCCCEECCCCC
GHIDQLKKIVDDLSRGVDDRGEILNFYNPGEFHMGCLRPCMYSHHFSLLGDTLYLNSTQR
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
SCDVPLGLNFNMVQVYVFLALMAQITGKKPGLAYHKIVNAHIYQDQLELMRDVQLKREPF
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PAPQFHINPKIKTLQDLETWVTLDDFDVTGYQFHDPIQYPFSV
CCCCEEECCCHHHHHHHHHHEEEECCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA