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The map label for this gene is ispH [H]

Identifier: 147673232

GI number: 147673232

Start: 223476

End: 224438

Strand: Direct

Name: ispH [H]

Synonym: VC0395_A0217

Alternate gene names: 147673232

Gene position: 223476-224438 (Clockwise)

Preceding gene: 147674041

Following gene: 147674079

Centisome position: 7.39

GC content: 50.67

Gene sequence:

>963_bases
ATGAGCAAACCAATGAAAATACTGTTAGCCAACCCTCGCGGTTTCTGCGCGGGTGTGGATCGCGCGATCAGCATTGTTGA
GCGTGCGCTGGAGCTTTATCAGCCGCCGATTTATGTGCGTCATGAAGTGGTGCACAACCGCTTTGTGGTCGAGGGTTTAA
AGCAGCGTGGAGCCCGCTTTGTTGAGGAATTAAATGAAGTTCCCGACGATAACATTGTGATTTTTTCGGCTCATGGCGTG
TCGCAAGCAGTTCGCCAAGAAGCGAAAGAGCGTTCACTGACGGTATTTGATGCGACTTGTCCACTGGTGACTAAAGTGCA
TATGGAAGTGGCGCGTGCCAGTCGCAAACATATGGAAGTAGTGTTGATTGGTCATGCTGGCCATCCTGAAGTGGAAGGCA
CGATGGGGCAGTATGCCAGTGATACAGGCGGCATGTATCTGGTCGAAAAGCCAGAAGATGTCGTATCACTGCAAGCTAAG
GTGAAAGATCCAAGTAACCTGCACTATGTGAGCCAAACAACGTTGTCAGTCGATGAAACTGCGGATGTGATTGATGAGCT
GCGCCGTGTATTTCCGAAGATCCAAGGCCCGCGTAAAGATGACATCTGTTATGCCACCCAAAACCGTCAAGATGCGGTGC
GTATCTTGGCTGAGCAAGTCGATGTGATGGTGGTGGTGGGCTCGAAAAACTCATCGAACTCGACTCGATTAAAGGAGTTG
GCTGAGAAGTTGGGCACTCCCGGTTACTTGATTGATTGCCCACAGGATATCGACCCTGGGGGGTTCGTTGATGCGCAGTT
AATTGGGGTGACTGCGGGCGCTTCGGCACCAGAAGAGTTGGTAAACCAAATCTTGGAACGCATCAAAGAGCTCGGCGCAA
CGTCAGTAGAAGAGGTGCTTGGCCGCGAAGAGAACATGTTCTTTGAAGTGCCTAAAGAGCTGCAAATTAAGCAAGTCGAC
TAG

Upstream 100 bases:

>100_bases
GATTTCAATCACCCATTGGCAGGGCAAGACATCACCTTTGATGTTGAGATCTTGTCTGTTGAGTGAGTTTCCTTACAATA
GCCAACCATAACGACTAGCA

Downstream 100 bases:

>100_bases
TCATTTCGCCATCCATCTTGTGAATCCAAAATGGATGGTTTCTTTGCAATTTCTTTGCAAAGGGATATCGCACAAAAAAA
TGGCTCCCAGATTGGGAGCC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSKPMKILLANPRGFCAGVDRAISIVERALELYQPPIYVRHEVVHNRFVVEGLKQRGARFVEELNEVPDDNIVIFSAHGV
SQAVRQEAKERSLTVFDATCPLVTKVHMEVARASRKHMEVVLIGHAGHPEVEGTMGQYASDTGGMYLVEKPEDVVSLQAK
VKDPSNLHYVSQTTLSVDETADVIDELRRVFPKIQGPRKDDICYATQNRQDAVRILAEQVDVMVVVGSKNSSNSTRLKEL
AEKLGTPGYLIDCPQDIDPGGFVDAQLIGVTAGASAPEELVNQILERIKELGATSVEEVLGREENMFFEVPKELQIKQVD

Sequences:

>Translated_320_residues
MSKPMKILLANPRGFCAGVDRAISIVERALELYQPPIYVRHEVVHNRFVVEGLKQRGARFVEELNEVPDDNIVIFSAHGV
SQAVRQEAKERSLTVFDATCPLVTKVHMEVARASRKHMEVVLIGHAGHPEVEGTMGQYASDTGGMYLVEKPEDVVSLQAK
VKDPSNLHYVSQTTLSVDETADVIDELRRVFPKIQGPRKDDICYATQNRQDAVRILAEQVDVMVVVGSKNSSNSTRLKEL
AEKLGTPGYLIDCPQDIDPGGFVDAQLIGVTAGASAPEELVNQILERIKELGATSVEEVLGREENMFFEVPKELQIKQVD
>Mature_319_residues
SKPMKILLANPRGFCAGVDRAISIVERALELYQPPIYVRHEVVHNRFVVEGLKQRGARFVEELNEVPDDNIVIFSAHGVS
QAVRQEAKERSLTVFDATCPLVTKVHMEVARASRKHMEVVLIGHAGHPEVEGTMGQYASDTGGMYLVEKPEDVVSLQAKV
KDPSNLHYVSQTTLSVDETADVIDELRRVFPKIQGPRKDDICYATQNRQDAVRILAEQVDVMVVVGSKNSSNSTRLKELA
EKLGTPGYLIDCPQDIDPGGFVDAQLIGVTAGASAPEELVNQILERIKELGATSVEEVLGREENMFFEVPKELQIKQVD

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=318, Percent_Identity=66.3522012578616, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35413; Mature: 35282

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPMKILLANPRGFCAGVDRAISIVERALELYQPPIYVRHEVVHNRFVVEGLKQRGARF
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VEELNEVPDDNIVIFSAHGVSQAVRQEAKERSLTVFDATCPLVTKVHMEVARASRKHMEV
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCEEE
VLIGHAGHPEVEGTMGQYASDTGGMYLVEKPEDVVSLQAKVKDPSNLHYVSQTTLSVDET
EEEECCCCCCCCCCHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECEECHHHH
ADVIDELRRVFPKIQGPRKDDICYATQNRQDAVRILAEQVDVMVVVGSKNSSNSTRLKEL
HHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
AEKLGTPGYLIDCPQDIDPGGFVDAQLIGVTAGASAPEELVNQILERIKELGATSVEEVL
HHHHCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHH
GREENMFFEVPKELQIKQVD
CCCCCEEEECCCCCCEEECC
>Mature Secondary Structure 
SKPMKILLANPRGFCAGVDRAISIVERALELYQPPIYVRHEVVHNRFVVEGLKQRGARF
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VEELNEVPDDNIVIFSAHGVSQAVRQEAKERSLTVFDATCPLVTKVHMEVARASRKHMEV
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCEEE
VLIGHAGHPEVEGTMGQYASDTGGMYLVEKPEDVVSLQAKVKDPSNLHYVSQTTLSVDET
EEEECCCCCCCCCCHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECEECHHHH
ADVIDELRRVFPKIQGPRKDDICYATQNRQDAVRILAEQVDVMVVVGSKNSSNSTRLKEL
HHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
AEKLGTPGYLIDCPQDIDPGGFVDAQLIGVTAGASAPEELVNQILERIKELGATSVEEVL
HHHHCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHH
GREENMFFEVPKELQIKQVD
CCCCCEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]