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The map label for this gene is tyrS-2

Identifier: 147673048

GI number: 147673048

Start: 162816

End: 164003

Strand: Direct

Name: tyrS-2

Synonym: VC0395_A0160

Alternate gene names: 147673048

Gene position: 162816-164003 (Clockwise)

Preceding gene: 147674212

Following gene: 147675665

Centisome position: 5.38

GC content: 47.47

Gene sequence:

>1188_bases
ATGGCGAGTATTGAAGCCGCTTTAGCCGAGATTAAACGTGGTGTTGAAGAGCTGATCCCCGAAGAAGAGCTCATTGCCAA
ACTCAAAGAAAATCGTCCGCTACGAATTAAGTTGGGGGCGGATCCAACGGCTCCGGATATCCATCTAGGCCACACGGTAA
TCCTCAATAAGCTTCGTACTTTCCAGGATTTAGGTCATGACGTGACATTTTTGATTGGTGACTTTACTGGAATGGTGGGG
GATCCAACAGGAAAAAACACCACACGCCCACCTTTGACGCGCGAAGATGTATTGCGTAACGCGGAAACGTACAAGCAGCA
AGTGTTCAAGATCCTCGATCCTGCCAAAACCAAGATCCAATTTAACTCAGAGTGGTTGTCAAAACTGGGAGCGGAAGGGA
TGATCCGTCTGGCTTCAAACCAGACCGTCGCTCGTATGTTAGAACGTGATGACTTTAAAAAGCGTTACAACAATGGTCAG
CCGATTGCGATTCACGAGTTTATGTACCCATTGCTACAAGGCTACGACTCTGTTGCGATGGAGACGGATGTTGAGCTAGG
CGGTACTGACCAGAAGTTTAACTTGCTGATGGGGCGTGAGCTACAAAAAGCCAACGGCCAAAAACCACAAGTGGTATTGA
TGATGCCTCTGCTGGTGGGATTGGATGGCGAGAAGAAAATGTCCAAATCAGCGCATAACTATATTGGTGTGAGCGAAGCG
CCGAGCGAAATGTTCGGTAAAATCATGTCAATTTCTGATGATTTGATGTGGAGCTACTACGAGCTGCTCTCTTTCCGTCC
GTTAGAAGAAGTGGCCCAATTCAAAGCAGAGGTTGCTAATGGTGCTAACCCGCGTGATATCAAGATTTTGCTAGCAAAAG
AGATCATCGCTCGTTTCCATTCTCAAGCCGACGCTGATGCAGCAGAACAAGAGTTCATCAACCGTTTCCAAAAAGGGGCG
ATGCCTGAGGAAATGCCAGAGTTTGAATTCGAGTCTGGAATCGCTATCTCCAATCTGCTTAAAGAGGCGGGACTTGTGGC
TTCTACTTCTGATGCACTGCGCATGATTAAACAAGGTGCGGTGAAGCTTGATGGCGAGAAGTTAGAAGATGCGAAGCTGA
TTCCTGCTTGTGGTACATCGGTTTACCAAGTGGGTAAGCGTAAGTTCGCTCGAGTGACCATCAAGTAA

Upstream 100 bases:

>100_bases
AAATCGCTGTCGGGTAGTCTAACTGGTTTCAAAATGCATCGCTATTCAAGTAATATGTCCCACTATCATATTTTTGCCAA
ATCTGTGGGAGTGAACAAGA

Downstream 100 bases:

>100_bases
TGGTTTTCAAACCTGAGCTAAAAATAAATAAAAAGCGCCAAACGGCGCTTTTTATTTTTCAGGTAGCCTAGTTTGGCTTA
GATGCCAAACGATTAAACTG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 2; TyrRS 2

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MASIEAALAEIKRGVEELIPEEELIAKLKENRPLRIKLGADPTAPDIHLGHTVILNKLRTFQDLGHDVTFLIGDFTGMVG
DPTGKNTTRPPLTREDVLRNAETYKQQVFKILDPAKTKIQFNSEWLSKLGAEGMIRLASNQTVARMLERDDFKKRYNNGQ
PIAIHEFMYPLLQGYDSVAMETDVELGGTDQKFNLLMGRELQKANGQKPQVVLMMPLLVGLDGEKKMSKSAHNYIGVSEA
PSEMFGKIMSISDDLMWSYYELLSFRPLEEVAQFKAEVANGANPRDIKILLAKEIIARFHSQADADAAEQEFINRFQKGA
MPEEMPEFEFESGIAISNLLKEAGLVASTSDALRMIKQGAVKLDGEKLEDAKLIPACGTSVYQVGKRKFARVTIK

Sequences:

>Translated_395_residues
MASIEAALAEIKRGVEELIPEEELIAKLKENRPLRIKLGADPTAPDIHLGHTVILNKLRTFQDLGHDVTFLIGDFTGMVG
DPTGKNTTRPPLTREDVLRNAETYKQQVFKILDPAKTKIQFNSEWLSKLGAEGMIRLASNQTVARMLERDDFKKRYNNGQ
PIAIHEFMYPLLQGYDSVAMETDVELGGTDQKFNLLMGRELQKANGQKPQVVLMMPLLVGLDGEKKMSKSAHNYIGVSEA
PSEMFGKIMSISDDLMWSYYELLSFRPLEEVAQFKAEVANGANPRDIKILLAKEIIARFHSQADADAAEQEFINRFQKGA
MPEEMPEFEFESGIAISNLLKEAGLVASTSDALRMIKQGAVKLDGEKLEDAKLIPACGTSVYQVGKRKFARVTIK
>Mature_394_residues
ASIEAALAEIKRGVEELIPEEELIAKLKENRPLRIKLGADPTAPDIHLGHTVILNKLRTFQDLGHDVTFLIGDFTGMVGD
PTGKNTTRPPLTREDVLRNAETYKQQVFKILDPAKTKIQFNSEWLSKLGAEGMIRLASNQTVARMLERDDFKKRYNNGQP
IAIHEFMYPLLQGYDSVAMETDVELGGTDQKFNLLMGRELQKANGQKPQVVLMMPLLVGLDGEKKMSKSAHNYIGVSEAP
SEMFGKIMSISDDLMWSYYELLSFRPLEEVAQFKAEVANGANPRDIKILLAKEIIARFHSQADADAAEQEFINRFQKGAM
PEEMPEFEFESGIAISNLLKEAGLVASTSDALRMIKQGAVKLDGEKLEDAKLIPACGTSVYQVGKRKFARVTIK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=416, Percent_Identity=24.7596153846154, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1787925, Length=407, Percent_Identity=26.044226044226, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25145802, Length=216, Percent_Identity=30.0925925925926, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=388, Percent_Identity=24.2268041237113, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130383, Length=402, Percent_Identity=25.3731343283582, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY2_VIBCH (Q9KU92)

Other databases:

- EMBL:   AE003852
- PIR:   G82298
- RefSeq:   NP_230280.1
- ProteinModelPortal:   Q9KU92
- SMR:   Q9KU92
- GeneID:   2615419
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0631
- TIGR:   VC_0631
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 44075; Mature: 43944

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 229-229

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIEAALAEIKRGVEELIPEEELIAKLKENRPLRIKLGADPTAPDIHLGHTVILNKLRT
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQDLGHDVTFLIGDFTGMVGDPTGKNTTRPPLTREDVLRNAETYKQQVFKILDPAKTKIQ
HHHCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEE
FNSEWLSKLGAEGMIRLASNQTVARMLERDDFKKRYNNGQPIAIHEFMYPLLQGYDSVAM
ECHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCHHHE
ETDVELGGTDQKFNLLMGRELQKANGQKPQVVLMMPLLVGLDGEKKMSKSAHNYIGVSEA
ECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCHHHHHHHHHCCCCCCCC
PSEMFGKIMSISDDLMWSYYELLSFRPLEEVAQFKAEVANGANPRDIKILLAKEIIARFH
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SQADADAAEQEFINRFQKGAMPEEMPEFEFESGIAISNLLKEAGLVASTSDALRMIKQGA
HHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCC
VKLDGEKLEDAKLIPACGTSVYQVGKRKFARVTIK
CEECCCCCCCCCCCCCCCHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
ASIEAALAEIKRGVEELIPEEELIAKLKENRPLRIKLGADPTAPDIHLGHTVILNKLRT
CHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQDLGHDVTFLIGDFTGMVGDPTGKNTTRPPLTREDVLRNAETYKQQVFKILDPAKTKIQ
HHHCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEE
FNSEWLSKLGAEGMIRLASNQTVARMLERDDFKKRYNNGQPIAIHEFMYPLLQGYDSVAM
ECHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCHHHE
ETDVELGGTDQKFNLLMGRELQKANGQKPQVVLMMPLLVGLDGEKKMSKSAHNYIGVSEA
ECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCHHHHHHHHHCCCCCCCC
PSEMFGKIMSISDDLMWSYYELLSFRPLEEVAQFKAEVANGANPRDIKILLAKEIIARFH
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SQADADAAEQEFINRFQKGAMPEEMPEFEFESGIAISNLLKEAGLVASTSDALRMIKQGA
HHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCC
VKLDGEKLEDAKLIPACGTSVYQVGKRKFARVTIK
CEECCCCCCCCCCCCCCCHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301