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The map label for this gene is cobB2 [H]

Identifier: 146343837

GI number: 146343837

Start: 7400973

End: 7401854

Strand: Reverse

Name: cobB2 [H]

Synonym: BRADO7087

Alternate gene names: 146343837

Gene position: 7401854-7400973 (Counterclockwise)

Preceding gene: 146343839

Following gene: 146343832

Centisome position: 99.27

GC content: 68.25

Gene sequence:

>882_bases
ATGCCGGCGCGTCGGTGCTGCGACGGTCTGGCGCAGCCCGGTTCCGGCGACTACATGTGCGAGGTCATGGTCGATCCCAC
AGCGCTGAAATCCTTCCTCGACAGCCACAGCCGCATCGTCGTCCTCACCGGCGCCGGCTGCAGCACCAATTCCGGCATTC
CCGATTATCGCGACACCGACGGCCAGTGGAAGCGCACGCCGCCAGTGACCTATCAGGCCTTCATGGGCGGCGAAGATACG
CGCCGGCGCTATTGGGCGCGCAGCATGGTCGGCTGGCGCCGCTTCGGCCGCGCCCAGCCGAACGGCGCGCATCACGCGCT
GGCAAGGCTGGAGGCGCAGGGCAAAAGCTCTCTGCTGGTGACACAGAACGTCGACCGGCTGCATCAGGCCGCCGGCGCCA
GCAATGTCATCGACCTGCACGGCCGGCTCGATCGCGTGCGCTGCATGGGCTGCGGCGCGACCTTGTCGCGCGCCGAATTC
CAGGACGAACTGGCGCACGCCAATCCGCACTGGCTGGCGCATGATGCCGCTGACGCGCCTGATGGCGATGCCGATCTGGA
CGGCGTCGATTTCGCCGCCTTTGTTGTGCCCGCGTGCCGCGCCTGCGGCGGCATCCTCAAACCGGATGTCGTGTTCTTCG
GCGAGACCGTGCCGCGCGACATCGTCGCCACCGCGCGCGATCACGTCGATCGCGCCGACGCCATGCTCGTCGTGGGCACC
TCGCTGATGGTCTATTCCGGCTTCCGCTTCGTCCGCGCCGCCGCCGACCGCAACATCCCGATCGCCGCCGTCAATCTCGG
CCGCACCCGCGCGGATGATCTGCTCTCGCTGAAGGTCGAAGCGCCGTGCGACGAGGCGCTGGCGTTCTTGTTGGAGGGGT
AG

Upstream 100 bases:

>100_bases
CGTCCGGCTCGTCCAGCGGCCAAAGGCCGCGGTCGTGTCCGTTGCGTGGCGTCATGGCAGGTCCTCCCGGCGGGACAAGC
GCTGCGGCCCTCATTGGTTC

Downstream 100 bases:

>100_bases
ACCTACACTCCCTCAGCTGTCATGGCCGGGCTTGACCCGGCCATCCATCCCTCTTCGCAAGGCGATGGATGCCCGGGCCT
TCGCCGCGCCGAAGCGCTTC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 2 [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPARRCCDGLAQPGSGDYMCEVMVDPTALKSFLDSHSRIVVLTGAGCSTNSGIPDYRDTDGQWKRTPPVTYQAFMGGEDT
RRRYWARSMVGWRRFGRAQPNGAHHALARLEAQGKSSLLVTQNVDRLHQAAGASNVIDLHGRLDRVRCMGCGATLSRAEF
QDELAHANPHWLAHDAADAPDGDADLDGVDFAAFVVPACRACGGILKPDVVFFGETVPRDIVATARDHVDRADAMLVVGT
SLMVYSGFRFVRAAADRNIPIAAVNLGRTRADDLLSLKVEAPCDEALAFLLEG

Sequences:

>Translated_293_residues
MPARRCCDGLAQPGSGDYMCEVMVDPTALKSFLDSHSRIVVLTGAGCSTNSGIPDYRDTDGQWKRTPPVTYQAFMGGEDT
RRRYWARSMVGWRRFGRAQPNGAHHALARLEAQGKSSLLVTQNVDRLHQAAGASNVIDLHGRLDRVRCMGCGATLSRAEF
QDELAHANPHWLAHDAADAPDGDADLDGVDFAAFVVPACRACGGILKPDVVFFGETVPRDIVATARDHVDRADAMLVVGT
SLMVYSGFRFVRAAADRNIPIAAVNLGRTRADDLLSLKVEAPCDEALAFLLEG
>Mature_292_residues
PARRCCDGLAQPGSGDYMCEVMVDPTALKSFLDSHSRIVVLTGAGCSTNSGIPDYRDTDGQWKRTPPVTYQAFMGGEDTR
RRYWARSMVGWRRFGRAQPNGAHHALARLEAQGKSSLLVTQNVDRLHQAAGASNVIDLHGRLDRVRCMGCGATLSRAEFQ
DELAHANPHWLAHDAADAPDGDADLDGVDFAAFVVPACRACGGILKPDVVFFGETVPRDIVATARDHVDRADAMLVVGTS
LMVYSGFRFVRAAADRNIPIAAVNLGRTRADDLLSLKVEAPCDEALAFLLEG

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912662, Length=292, Percent_Identity=44.8630136986301, Blast_Score=240, Evalue=9e-64,
Organism=Homo sapiens, GI6912664, Length=292, Percent_Identity=29.7945205479452, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI13787215, Length=281, Percent_Identity=30.2491103202847, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI300795542, Length=290, Percent_Identity=28.6206896551724, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI6912660, Length=239, Percent_Identity=28.4518828451883, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI63054862, Length=234, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI308199517, Length=274, Percent_Identity=31.3868613138686, Blast_Score=98, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71990482, Length=278, Percent_Identity=41.0071942446043, Blast_Score=211, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI71990487, Length=280, Percent_Identity=40.7142857142857, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17567771, Length=275, Percent_Identity=37.8181818181818, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17541892, Length=245, Percent_Identity=26.1224489795918, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28571445, Length=273, Percent_Identity=44.3223443223443, Blast_Score=217, Evalue=7e-57,
Organism=Drosophila melanogaster, GI28571443, Length=221, Percent_Identity=43.8914027149321, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI28571441, Length=221, Percent_Identity=43.8914027149321, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI17137536, Length=230, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 31696; Mature: 31565

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARRCCDGLAQPGSGDYMCEVMVDPTALKSFLDSHSRIVVLTGAGCSTNSGIPDYRDTD
CCHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCC
GQWKRTPPVTYQAFMGGEDTRRRYWARSMVGWRRFGRAQPNGAHHALARLEAQGKSSLLV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEE
TQNVDRLHQAAGASNVIDLHGRLDRVRCMGCGATLSRAEFQDELAHANPHWLAHDAADAP
ECCHHHHHHHCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCC
DGDADLDGVDFAAFVVPACRACGGILKPDVVFFGETVPRDIVATARDHVDRADAMLVVGT
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCEEEEECC
SLMVYSGFRFVRAAADRNIPIAAVNLGRTRADDLLSLKVEAPCDEALAFLLEG
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure 
PARRCCDGLAQPGSGDYMCEVMVDPTALKSFLDSHSRIVVLTGAGCSTNSGIPDYRDTD
CHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCC
GQWKRTPPVTYQAFMGGEDTRRRYWARSMVGWRRFGRAQPNGAHHALARLEAQGKSSLLV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEE
TQNVDRLHQAAGASNVIDLHGRLDRVRCMGCGATLSRAEFQDELAHANPHWLAHDAADAP
ECCHHHHHHHCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCC
DGDADLDGVDFAAFVVPACRACGGILKPDVVFFGETVPRDIVATARDHVDRADAMLVVGT
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCEEEEECC
SLMVYSGFRFVRAAADRNIPIAAVNLGRTRADDLLSLKVEAPCDEALAFLLEG
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]