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The map label for this gene is crp [C]

Identifier: 146343726

GI number: 146343726

Start: 7262538

End: 7263215

Strand: Direct

Name: crp [C]

Synonym: BRADO6973

Alternate gene names: 146343726

Gene position: 7262538-7263215 (Clockwise)

Preceding gene: 146343723

Following gene: 146343727

Centisome position: 97.4

GC content: 65.93

Gene sequence:

>678_bases
GTGATGAGCAAGCAGGCCGAATTTGCGGTCATTCTGAAGATGAACCCGATGTTCGCTGATCTCGGCTCGGACGAGCTGCA
GCGCATTTCGAACCTGTGCCACACCCAGCATCTCTCGGCGGGCGAGGTGTTGTTCCAGAAGGGCGATCCCGGCAACGCAC
TGTTCGGCGTCCGCCGCGGCCAGATCCGGATCGAGACCGGCGCCACCGACGGCTCGCGGCTGACGCTCAATTTCATGGGC
CCCGGCGATCTGTTCGGCGAGGTCGCGGTGCTCGACGGCCAGGAGCGCACCGCTGACGCCACGGCGGGTGAGGCCACCGA
GCTGTTCGTGCTCCGGCGCGAGGATTTCCTGAACTTCCTGGAGCGCGAGCCCAAGGTCGCGATCAAGATCATCCAGCTGT
TGTGCCAGCGCATCCGCTGGCAGAGCGAGCGGATGGAGGAATCGGTATTGCAGCCGCTGCCGATCCGGCTGGCCCGCCGG
CTCTGCGCGCTCGCGGAGGATTTCGGCTCCGAGGTGCACATCTCGCAGGAGCAGCTCGGCGTGTTCGTCGGCGCCGCGCG
CGAGAGCGTCAACCGCCAGCTCCAGGCCTGGCGCAAGGACAAGATCCTAGACCTGCAGCGCGGCCGCATCCTGCTGCACG
ACCTGACGAAGCTGAACACGATCGCGCGCAACGAATAG

Upstream 100 bases:

>100_bases
CCAAACTAGTCGTGAGCCCATGCGGCGTTATGTGACTTACGACACACTTCCGCCAGAGCGGATCCTGTAACTGGGGCTTT
GAACCACTGCCGAAGGTATA

Downstream 100 bases:

>100_bases
CGGCCGACCCGGACGATGTTGTCCACTTCTCGTCGGGCTGCTGGCGAGAACGATTCGCAATGTATCGTTAGGTGTCAGAC
TCGGCCAAACATGATCGCAA

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MMSKQAEFAVILKMNPMFADLGSDELQRISNLCHTQHLSAGEVLFQKGDPGNALFGVRRGQIRIETGATDGSRLTLNFMG
PGDLFGEVAVLDGQERTADATAGEATELFVLRREDFLNFLEREPKVAIKIIQLLCQRIRWQSERMEESVLQPLPIRLARR
LCALAEDFGSEVHISQEQLGVFVGAARESVNRQLQAWRKDKILDLQRGRILLHDLTKLNTIARNE

Sequences:

>Translated_225_residues
MMSKQAEFAVILKMNPMFADLGSDELQRISNLCHTQHLSAGEVLFQKGDPGNALFGVRRGQIRIETGATDGSRLTLNFMG
PGDLFGEVAVLDGQERTADATAGEATELFVLRREDFLNFLEREPKVAIKIIQLLCQRIRWQSERMEESVLQPLPIRLARR
LCALAEDFGSEVHISQEQLGVFVGAARESVNRQLQAWRKDKILDLQRGRILLHDLTKLNTIARNE
>Mature_225_residues
MMSKQAEFAVILKMNPMFADLGSDELQRISNLCHTQHLSAGEVLFQKGDPGNALFGVRRGQIRIETGATDGSRLTLNFMG
PGDLFGEVAVLDGQERTADATAGEATELFVLRREDFLNFLEREPKVAIKIIQLLCQRIRWQSERMEESVLQPLPIRLARR
LCALAEDFGSEVHISQEQLGVFVGAARESVNRQLQAWRKDKILDLQRGRILLHDLTKLNTIARNE

Specific function: This Protein Complexes With Cyclic AMP And Binds To Specific DNA Sites Near The Promoter To Regulate The Transcription Of Several Catabolite-Sensitive Operons. The Protein Induces A Severe Bend In The DNA. Acts As A Negative Regulator Of Its Own Synthesi

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cyclic nucleotide-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=189, Percent_Identity=21.6931216931217, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR005821
- InterPro:   IPR003091
- InterPro:   IPR014710 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00520 Ion_trans [H]

EC number: NA

Molecular weight: Translated: 25369; Mature: 25369

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSKQAEFAVILKMNPMFADLGSDELQRISNLCHTQHLSAGEVLFQKGDPGNALFGVRRG
CCCCCCCEEEEEEECCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEECC
QIRIETGATDGSRLTLNFMGPGDLFGEVAVLDGQERTADATAGEATELFVLRREDFLNFL
EEEEEECCCCCCEEEEEECCCCHHHCCEEEECCCCCCCCCCCCCCHHEEEEEHHHHHHHH
EREPKVAIKIIQLLCQRIRWQSERMEESVLQPLPIRLARRLCALAEDFGSEVHISQEQLG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECHHHHH
VFVGAARESVNRQLQAWRKDKILDLQRGRILLHDLTKLNTIARNE
HHHHHHHHHHHHHHHHHHHCCCEECCCCEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMSKQAEFAVILKMNPMFADLGSDELQRISNLCHTQHLSAGEVLFQKGDPGNALFGVRRG
CCCCCCCEEEEEEECCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEECC
QIRIETGATDGSRLTLNFMGPGDLFGEVAVLDGQERTADATAGEATELFVLRREDFLNFL
EEEEEECCCCCCEEEEEECCCCHHHCCEEEECCCCCCCCCCCCCCHHEEEEEHHHHHHHH
EREPKVAIKIIQLLCQRIRWQSERMEESVLQPLPIRLARRLCALAEDFGSEVHISQEQLG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECHHHHH
VFVGAARESVNRQLQAWRKDKILDLQRGRILLHDLTKLNTIARNE
HHHHHHHHHHHHHHHHHHHCCCEECCCCEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1522059 [H]