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The map label for this gene is intA [H]

Identifier: 146343698

GI number: 146343698

Start: 7229043

End: 7230233

Strand: Direct

Name: intA [H]

Synonym: BRADO6943

Alternate gene names: 146343698

Gene position: 7229043-7230233 (Clockwise)

Preceding gene: 146343697

Following gene: 146343700

Centisome position: 96.95

GC content: 58.27

Gene sequence:

>1191_bases
ATGGCTGGCAAGCTTACTGCCCGTAAGGTTGAGACGGCAAAGCCGGGCAAATATAGCGACGGCGGCAACCTCTATCTGAT
CGTTTCCGAAACTGGTGCTCGCAAATGGGTTCTGCGCTTCACCTGGCGCGGTCAAGCGAAAGAGATGGGCTTGGGTAGCG
CGGCTAGCGTCCCACTCTCTGACGCCCGCGAGAAGGCTGCGGATGCACGGCGGACGATTGGCAGAGGGCTAAATCCAATC
GAGGAGCGCAGGCGGGGCGGTATCACCCCGACTTTCGGAGAACTGGCCGATGAAGTGCGCGAGTCGCTCTCCACCGGGTT
TCGCAACCAAAAACATAAGGCCCAATGGAAGTCGACGCTGGAGACCTATGCTTCGTCATTGCGGGCAAAGCCCGTCGACA
CAATCACCACGGAAGATTTGCTCGCAGCCCTTAAACCCATTTGGACCACAAAGGCAGAAACTGCCTCCCGGCTCCGGGGG
CGGATCGAGAAGGTTTTAGACGCTGCCAAGGCAAAAGGTTTCCGCGATGGTGAAAATCCCGCACGGTGGCGGGGCCATTT
GGACCATCTGCTGCCGAAGCCGTCCAAGCTCGCGCGGGGGCACCATCCGGCAATGCCATATGAGAGTGTGCCTTCTTTCA
TAGGCGAGCTACGGCAGCGCGAGGCAACCGCCGCGCTTGCGTTGGAGCTTTGCATACTGACTACTGCCCGTTCTGGTGAG
ATACTGGGGATGAGATGGTCAGAAATCGACTTGGAAAAAAAGGTCTGGATCGTTCCGCCTGAGCGCATGAAGGCCGGACG
GGAACACCGCGTTCCTCTGTGCTCCCGAGCAGTCGCAATCCTAAGTGGCCTTGAAGCAGTAAAGAACGGCGAGTTCGTCT
TTCAAGGGAAGGCTCGCGGCAAGCCGCTCTCCAACATGGCTATGGAGATGGTACTGCGCCGAATGAAAGTTGCGGAAGCG
ACGGTGCACGGCTTTCGCTCCAGCTTTAGAGATTGGGCTGGCAATGTCTCCAGCTTTCCGCGCGAAATTGTCGAGACCGC
GCTGGCACATGTCATTGGTGACAAAGCAGAGCAAGCCTACCGCCGTAGCGATGCACTCGAAAAGCGCCGCAAGCTCATGG
ATGCATGGGCAGGCTATTGTGAGCCAAGAGCTAGGGGCAACGTCGTGGCTCTTGCACGCTCAGCACCCTAA

Upstream 100 bases:

>100_bases
ATAGGGCGTTTCCTGTCGTCGAGGTTCACCGCGGTTTGTATCCATTCGCCCAAAACCGTGCTAATTTTGGGGACCGATCT
GGGGACCGAAGGAGACGAAC

Downstream 100 bases:

>100_bases
GCCTCGCCTCCGCGCTTCCTTGCCTTAGCTTTCCGCCGCTGAAGCGAATTCCAGATCATCACCTTGCGCCCGACCTGACT
GCCTATTCCAATTGCAATGA

Product: putative phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAGKLTARKVETAKPGKYSDGGNLYLIVSETGARKWVLRFTWRGQAKEMGLGSAASVPLSDAREKAADARRTIGRGLNPI
EERRRGGITPTFGELADEVRESLSTGFRNQKHKAQWKSTLETYASSLRAKPVDTITTEDLLAALKPIWTTKAETASRLRG
RIEKVLDAAKAKGFRDGENPARWRGHLDHLLPKPSKLARGHHPAMPYESVPSFIGELRQREATAALALELCILTTARSGE
ILGMRWSEIDLEKKVWIVPPERMKAGREHRVPLCSRAVAILSGLEAVKNGEFVFQGKARGKPLSNMAMEMVLRRMKVAEA
TVHGFRSSFRDWAGNVSSFPREIVETALAHVIGDKAEQAYRRSDALEKRRKLMDAWAGYCEPRARGNVVALARSAP

Sequences:

>Translated_396_residues
MAGKLTARKVETAKPGKYSDGGNLYLIVSETGARKWVLRFTWRGQAKEMGLGSAASVPLSDAREKAADARRTIGRGLNPI
EERRRGGITPTFGELADEVRESLSTGFRNQKHKAQWKSTLETYASSLRAKPVDTITTEDLLAALKPIWTTKAETASRLRG
RIEKVLDAAKAKGFRDGENPARWRGHLDHLLPKPSKLARGHHPAMPYESVPSFIGELRQREATAALALELCILTTARSGE
ILGMRWSEIDLEKKVWIVPPERMKAGREHRVPLCSRAVAILSGLEAVKNGEFVFQGKARGKPLSNMAMEMVLRRMKVAEA
TVHGFRSSFRDWAGNVSSFPREIVETALAHVIGDKAEQAYRRSDALEKRRKLMDAWAGYCEPRARGNVVALARSAP
>Mature_395_residues
AGKLTARKVETAKPGKYSDGGNLYLIVSETGARKWVLRFTWRGQAKEMGLGSAASVPLSDAREKAADARRTIGRGLNPIE
ERRRGGITPTFGELADEVRESLSTGFRNQKHKAQWKSTLETYASSLRAKPVDTITTEDLLAALKPIWTTKAETASRLRGR
IEKVLDAAKAKGFRDGENPARWRGHLDHLLPKPSKLARGHHPAMPYESVPSFIGELRQREATAALALELCILTTARSGEI
LGMRWSEIDLEKKVWIVPPERMKAGREHRVPLCSRAVAILSGLEAVKNGEFVFQGKARGKPLSNMAMEMVLRRMKVAEAT
VHGFRSSFRDWAGNVSSFPREIVETALAHVIGDKAEQAYRRSDALEKRRKLMDAWAGYCEPRARGNVVALARSAP

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=407, Percent_Identity=27.7641277641278, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693166, Length=402, Percent_Identity=29.1044776119403, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1788690, Length=405, Percent_Identity=25.1851851851852, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 43914; Mature: 43783

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKLTARKVETAKPGKYSDGGNLYLIVSETGARKWVLRFTWRGQAKEMGLGSAASVPLS
CCCCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHCCCCCCCCCCHH
DAREKAADARRTIGRGLNPIEERRRGGITPTFGELADEVRESLSTGFRNQKHKAQWKSTL
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ETYASSLRAKPVDTITTEDLLAALKPIWTTKAETASRLRGRIEKVLDAAKAKGFRDGENP
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ARWRGHLDHLLPKPSKLARGHHPAMPYESVPSFIGELRQREATAALALELCILTTARSGE
HHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ILGMRWSEIDLEKKVWIVPPERMKAGREHRVPLCSRAVAILSGLEAVKNGEFVFQGKARG
EEECCCCCCCCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
KPLSNMAMEMVLRRMKVAEATVHGFRSSFRDWAGNVSSFPREIVETALAHVIGDKAEQAY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRSDALEKRRKLMDAWAGYCEPRARGNVVALARSAP
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
AGKLTARKVETAKPGKYSDGGNLYLIVSETGARKWVLRFTWRGQAKEMGLGSAASVPLS
CCCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHCCCCCCCCCCHH
DAREKAADARRTIGRGLNPIEERRRGGITPTFGELADEVRESLSTGFRNQKHKAQWKSTL
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ETYASSLRAKPVDTITTEDLLAALKPIWTTKAETASRLRGRIEKVLDAAKAKGFRDGENP
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ARWRGHLDHLLPKPSKLARGHHPAMPYESVPSFIGELRQREATAALALELCILTTARSGE
HHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ILGMRWSEIDLEKKVWIVPPERMKAGREHRVPLCSRAVAILSGLEAVKNGEFVFQGKARG
EEECCCCCCCCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
KPLSNMAMEMVLRRMKVAEATVHGFRSSFRDWAGNVSSFPREIVETALAHVIGDKAEQAY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRSDALEKRRKLMDAWAGYCEPRARGNVVALARSAP
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]