Click here to switch to the map view.

The map label for this gene is livK [C]

Identifier: 146343643

GI number: 146343643

Start: 7151314

End: 7152546

Strand: Direct

Name: livK [C]

Synonym: BRADO6883

Alternate gene names: 146343643

Gene position: 7151314-7152546 (Clockwise)

Preceding gene: 146343642

Following gene: 146343645

Centisome position: 95.91

GC content: 61.23

Gene sequence:

>1233_bases
ATGTTGCCGATGACGACCAGGACTCTGCTGTCTTTCGCGGCCGCCGCCGCGTTCTCCGTTCTGGCGAGCCACAGCGCGCT
CGCCCAGAAGAAATACGACACCGGCGCCACCGACACCGAGATCAAGATCGGCAATGTCGAGGCCTATAGCGGCCCCGCCT
CGGCCTACGGCATCATCGGCAAGACCGAGGAAGCCTATTTCAAGATGATCAACGATCAGGGCGGGGTGAACGGCCGCAAG
ATCAACTTCATCTCCTATGACGACGGCTACAGCCCGCCCAAGACGGTCGAGCAGGTGCGCAAGCTGATCGAGAGCGACGA
GGTGTTCCTGGTGTTCAACGCGCTGGGCACGCCGACCCAGACCGCCGTGCAGAAGTACCACAATTCCAAGAAGGTGCCGC
AGCTGTTCCTCGCCACCGGCGCCAGCAAGTGGAACGACCCCAAGGACTTTCCGTGGACGATGGGCTTCCAGCCCAGCTAC
CGCGTCGAGGCGCGGATCTTCGCCAAGTACATCCTGAAGGAAAAGCCGAACGCGAAGATTGCGATCTTCTATGCCAATGA
CGATTTCGGCAAGGACTACGTCCTCGGCATCAAGGAGGTGCTCGGCGACAAGGCCAAGACGATGCTCGTCGCCGAGGAGA
GCTACGAGACCTCGGAGCCGTCGATCGATGCTCACATCGTCAAGCTGAAAGGCACGGGCGCCGACGTGTTCGTCAACATC
GCCACGCCTAAATTCGCAGCGCAAGCGATCAAGAAGATCGCCGAGCTGGAATGGAAGCCGATGCATGTCATGACCGACGT
GTCGATCTCGATCGGCGCCGTGATGAAGCCGGCCGGCCTCGAGGCCTCCGAGGGCGTGCTGTCCGCCGGCTATCTCAAGG
ACCCGTCGGATCCGCAGTGGAAGGACGACGAGGGCATGAAGAAGTTCATGGCCTTCATCGACAAGTACATGCCCGGCGCC
AATATCTCCGACGCCAACCTCGCCTATGGCTACGCCGCCGCGCAGACCATGGTGCAGACCCTGAAGCAGTGCGGCGACAA
TCTCACCCGCGAGAACGTCATGAAGCAGGCCGCCAGCCTGAAGGACTTCGCGCCCGACACCCTGATCCCCGGCATCAAGG
TCAACACCGGCCCGAACGATTTCGCCCCGATCGAGCAGCTCAAGATGATGCAGTTCAAGGGCGGCAAATGGGACCTGTTC
GGGGACATCATCAGCGCGGATGTCGGGGGATAA

Upstream 100 bases:

>100_bases
TCGATCTGGTCCAGCAGGCGTCGTGATGTCAGATGCGCGGCCGACACCGCGCATCTCCGACCACCAAGACTCATAAGACC
AACCAGAGGAAGCCCAGGGA

Downstream 100 bases:

>100_bases
GCGAGGCGCGGCGCGCTAAGCCGTTGTAGCGCTGGCGCGCCGCATTCGCCCGATCGGCGCGCCCACGGCTGTCCCTACGC
AGCGCTGTTCACTTGAGCCG

Product: branched chain amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MLPMTTRTLLSFAAAAAFSVLASHSALAQKKYDTGATDTEIKIGNVEAYSGPASAYGIIGKTEEAYFKMINDQGGVNGRK
INFISYDDGYSPPKTVEQVRKLIESDEVFLVFNALGTPTQTAVQKYHNSKKVPQLFLATGASKWNDPKDFPWTMGFQPSY
RVEARIFAKYILKEKPNAKIAIFYANDDFGKDYVLGIKEVLGDKAKTMLVAEESYETSEPSIDAHIVKLKGTGADVFVNI
ATPKFAAQAIKKIAELEWKPMHVMTDVSISIGAVMKPAGLEASEGVLSAGYLKDPSDPQWKDDEGMKKFMAFIDKYMPGA
NISDANLAYGYAAAQTMVQTLKQCGDNLTRENVMKQAASLKDFAPDTLIPGIKVNTGPNDFAPIEQLKMMQFKGGKWDLF
GDIISADVGG

Sequences:

>Translated_410_residues
MLPMTTRTLLSFAAAAAFSVLASHSALAQKKYDTGATDTEIKIGNVEAYSGPASAYGIIGKTEEAYFKMINDQGGVNGRK
INFISYDDGYSPPKTVEQVRKLIESDEVFLVFNALGTPTQTAVQKYHNSKKVPQLFLATGASKWNDPKDFPWTMGFQPSY
RVEARIFAKYILKEKPNAKIAIFYANDDFGKDYVLGIKEVLGDKAKTMLVAEESYETSEPSIDAHIVKLKGTGADVFVNI
ATPKFAAQAIKKIAELEWKPMHVMTDVSISIGAVMKPAGLEASEGVLSAGYLKDPSDPQWKDDEGMKKFMAFIDKYMPGA
NISDANLAYGYAAAQTMVQTLKQCGDNLTRENVMKQAASLKDFAPDTLIPGIKVNTGPNDFAPIEQLKMMQFKGGKWDLF
GDIISADVGG
>Mature_410_residues
MLPMTTRTLLSFAAAAAFSVLASHSALAQKKYDTGATDTEIKIGNVEAYSGPASAYGIIGKTEEAYFKMINDQGGVNGRK
INFISYDDGYSPPKTVEQVRKLIESDEVFLVFNALGTPTQTAVQKYHNSKKVPQLFLATGASKWNDPKDFPWTMGFQPSY
RVEARIFAKYILKEKPNAKIAIFYANDDFGKDYVLGIKEVLGDKAKTMLVAEESYETSEPSIDAHIVKLKGTGADVFVNI
ATPKFAAQAIKKIAELEWKPMHVMTDVSISIGAVMKPAGLEASEGVLSAGYLKDPSDPQWKDDEGMKKFMAFIDKYMPGA
NISDANLAYGYAAAQTMVQTLKQCGDNLTRENVMKQAASLKDFAPDTLIPGIKVNTGPNDFAPIEQLKMMQFKGGKWDLF
GDIISADVGG

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 44780; Mature: 44780

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPMTTRTLLSFAAAAAFSVLASHSALAQKKYDTGATDTEIKIGNVEAYSGPASAYGIIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEECCCCCHHEECCC
KTEEAYFKMINDQGGVNGRKINFISYDDGYSPPKTVEQVRKLIESDEVFLVFNALGTPTQ
CCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHH
TAVQKYHNSKKVPQLFLATGASKWNDPKDFPWTMGFQPSYRVEARIFAKYILKEKPNAKI
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCEE
AIFYANDDFGKDYVLGIKEVLGDKAKTMLVAEESYETSEPSIDAHIVKLKGTGADVFVNI
EEEEECCCCCCHHHHHHHHHHCCCHHEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEE
ATPKFAAQAIKKIAELEWKPMHVMTDVSISIGAVMKPAGLEASEGVLSAGYLKDPSDPQW
CCHHHHHHHHHHHHHCCCCCEEEEEECCEEECCEECCCCCCCCCCCEECCCCCCCCCCCC
KDDEGMKKFMAFIDKYMPGANISDANLAYGYAAAQTMVQTLKQCGDNLTRENVMKQAASL
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KDFAPDTLIPGIKVNTGPNDFAPIEQLKMMQFKGGKWDLFGDIISADVGG
HHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHCCCCC
>Mature Secondary Structure
MLPMTTRTLLSFAAAAAFSVLASHSALAQKKYDTGATDTEIKIGNVEAYSGPASAYGIIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEECCCCCHHEECCC
KTEEAYFKMINDQGGVNGRKINFISYDDGYSPPKTVEQVRKLIESDEVFLVFNALGTPTQ
CCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHH
TAVQKYHNSKKVPQLFLATGASKWNDPKDFPWTMGFQPSYRVEARIFAKYILKEKPNAKI
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCEE
AIFYANDDFGKDYVLGIKEVLGDKAKTMLVAEESYETSEPSIDAHIVKLKGTGADVFVNI
EEEEECCCCCCHHHHHHHHHHCCCHHEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEE
ATPKFAAQAIKKIAELEWKPMHVMTDVSISIGAVMKPAGLEASEGVLSAGYLKDPSDPQW
CCHHHHHHHHHHHHHCCCCCEEEEEECCEEECCEECCCCCCCCCCCEECCCCCCCCCCCC
KDDEGMKKFMAFIDKYMPGANISDANLAYGYAAAQTMVQTLKQCGDNLTRENVMKQAASL
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KDFAPDTLIPGIKVNTGPNDFAPIEQLKMMQFKGGKWDLFGDIISADVGG
HHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA