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The map label for this gene is fsr [H]

Identifier: 146343622

GI number: 146343622

Start: 7124261

End: 7125484

Strand: Direct

Name: fsr [H]

Synonym: BRADO6861

Alternate gene names: 146343622

Gene position: 7124261-7125484 (Clockwise)

Preceding gene: 146343618

Following gene: 146343623

Centisome position: 95.54

GC content: 67.32

Gene sequence:

>1224_bases
ATGGCTGACGTTCTTGCCGCATCGCAACATAGCGGTGCTCCGGTCCGGGCCGCCTCCTCGCTGCGGACACTGGCGCTGAT
CTCGCTCGCCCATTGGGTCAGCCACCTGCACATGCTGGTGCTGCCGATGCTGTTTCCCTATCTCAAGCAGAAGCTCGGGG
TCGGCTATGTCGAGCTCGGCTTTCCGCTCACCGTCTTCGCGGTGGTCTCGGCGCTGACCCAGGCCCCGGTCGGCTATCTC
GTCGACCGCGCCGGCGCTCGCCGCATCCTGCTGCTGGGCCTGACGCTCGGCGGGCTTTCGCTCGCGATGCTCGGTGTCTA
CCTGAGCTACGCCAGCCTGGTCGTCTCGGCCGTGCTGCTCGGCCTCGCCAATGCGGTCTATCACCCCTCCGACTACGCGC
TGCTGTCGGCGCATATGGAAGAGAGCCGGATGGGCCGCGCCTTCGGCGTGCACACTTTTGCCGGCTATCTCGGCGGCGCA
GTCGCGCCGGCCGTCATGGCCGCGCTGGTCACCACCGTCGGCGGCTCCGGTGCGCTGATCGCAGCTGGCCTGGTCGGGCC
TCTCGCCGCGTTGCTGCTCCTGGTCGGCAACGTGCCGGAGGTGCCGCCGCATCCGCACGCCACCAAGGCTGATGGCACCA
AGGTCAAGCAGCCGGGCCTGCTGACGCCGATGCTGATGCTGCTGACCGTGTTCTTCATGCTGCTCAGCCTGTCCAACGCC
GGCATCTCCAGCTTTGGCGTGGTCGCCTTCATGAACGGCTACGGCATCTCGTTCTCGACCGCCAACATCGCGCTCACCGC
CTTCCTGGGCGCCAGCGCGGTCGGCGTGCTCGCCGGCGGCCAGCTCGCCGACCGCATCCGTCATCACAATCTGGTCGCTG
CGGTCGCCTATGCGATCAACGCCGTGCTGGTGCTGATCATCACCTTCGTCAGCCTGCACCCCGTGCTGCTGGTTGCGATC
ATGCTGACCGCGGGCTTCATGAGCGGCGTGATCGCGCCGTCGCGCGACATGCTGGTGCGCAACGCCGCCCCTCCCGGCGC
CGCCGGCCGCGCCTTCGGCATCGTCTCCACCGGCTTCAACTTCGCCGGCGTCGTCAGCCCGCTGCTGTTCGGCTGGATCA
TGGACATGAACGCCCCGCGCTGGGTGTTCGGCACGTCAGTGGTGTTCATGCTGCTGACCATCGTGCTGGCGTTCTTCACC
GAGCCGAAGAAGAAGATGACGTAG

Upstream 100 bases:

>100_bases
TGTGACGCTTCGTCGCGCCACGGCGCGAGAGCGCGCGACCGGGGGTGAGCCGTCGCGACAATCCAAACGCATATGGACCC
GGGAGCCGAACGGATCACGC

Downstream 100 bases:

>100_bases
CTGTCGTCGTGGGGTAGGCAGAGCTCAGCGTGCCCACCGCCAAGACCATCGCGGTGAACGGTGGGCACGCTTCGCTCCGC
CCACCCGACGCAAGGGCTCC

Product: major facilitator superfamily permease

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MADVLAASQHSGAPVRAASSLRTLALISLAHWVSHLHMLVLPMLFPYLKQKLGVGYVELGFPLTVFAVVSALTQAPVGYL
VDRAGARRILLLGLTLGGLSLAMLGVYLSYASLVVSAVLLGLANAVYHPSDYALLSAHMEESRMGRAFGVHTFAGYLGGA
VAPAVMAALVTTVGGSGALIAAGLVGPLAALLLLVGNVPEVPPHPHATKADGTKVKQPGLLTPMLMLLTVFFMLLSLSNA
GISSFGVVAFMNGYGISFSTANIALTAFLGASAVGVLAGGQLADRIRHHNLVAAVAYAINAVLVLIITFVSLHPVLLVAI
MLTAGFMSGVIAPSRDMLVRNAAPPGAAGRAFGIVSTGFNFAGVVSPLLFGWIMDMNAPRWVFGTSVVFMLLTIVLAFFT
EPKKKMT

Sequences:

>Translated_407_residues
MADVLAASQHSGAPVRAASSLRTLALISLAHWVSHLHMLVLPMLFPYLKQKLGVGYVELGFPLTVFAVVSALTQAPVGYL
VDRAGARRILLLGLTLGGLSLAMLGVYLSYASLVVSAVLLGLANAVYHPSDYALLSAHMEESRMGRAFGVHTFAGYLGGA
VAPAVMAALVTTVGGSGALIAAGLVGPLAALLLLVGNVPEVPPHPHATKADGTKVKQPGLLTPMLMLLTVFFMLLSLSNA
GISSFGVVAFMNGYGISFSTANIALTAFLGASAVGVLAGGQLADRIRHHNLVAAVAYAINAVLVLIITFVSLHPVLLVAI
MLTAGFMSGVIAPSRDMLVRNAAPPGAAGRAFGIVSTGFNFAGVVSPLLFGWIMDMNAPRWVFGTSVVFMLLTIVLAFFT
EPKKKMT
>Mature_406_residues
ADVLAASQHSGAPVRAASSLRTLALISLAHWVSHLHMLVLPMLFPYLKQKLGVGYVELGFPLTVFAVVSALTQAPVGYLV
DRAGARRILLLGLTLGGLSLAMLGVYLSYASLVVSAVLLGLANAVYHPSDYALLSAHMEESRMGRAFGVHTFAGYLGGAV
APAVMAALVTTVGGSGALIAAGLVGPLAALLLLVGNVPEVPPHPHATKADGTKVKQPGLLTPMLMLLTVFFMLLSLSNAG
ISSFGVVAFMNGYGISFSTANIALTAFLGASAVGVLAGGQLADRIRHHNLVAAVAYAINAVLVLIITFVSLHPVLLVAIM
LTAGFMSGVIAPSRDMLVRNAAPPGAAGRAFGIVSTGFNFAGVVSPLLFGWIMDMNAPRWVFGTSVVFMLLTIVLAFFTE
PKKKMT

Specific function: Confers the resistance against fosmidomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=307, Percent_Identity=25.0814332247557, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI2367379, Length=139, Percent_Identity=29.4964028776978, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42227; Mature: 42096

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
ADVLAASQHSGAPVRAASSLRTLALISLAHWVSHLHMLVLPMLFPYLKQKLGVGYVELG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
FPLTVFAVVSALTQAPVGYLVDRAGARRILLLGLTLGGLSLAMLGVYLSYASLVVSAVLL
CHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLANAVYHPSDYALLSAHMEESRMGRAFGVHTFAGYLGGAVAPAVMAALVTTVGGSGALI
HHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AAGLVGPLAALLLLVGNVPEVPPHPHATKADGTKVKQPGLLTPMLMLLTVFFMLLSLSNA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GISSFGVVAFMNGYGISFSTANIALTAFLGASAVGVLAGGQLADRIRHHNLVAAVAYAIN
CCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AVLVLIITFVSLHPVLLVAIMLTAGFMSGVIAPSRDMLVRNAAPPGAAGRAFGIVSTGFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCEEEEHHCCCC
FAGVVSPLLFGWIMDMNAPRWVFGTSVVFMLLTIVLAFFTEPKKKMT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8917080; 9278503 [H]