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The map label for this gene is gatA [H]

Identifier: 146343576

GI number: 146343576

Start: 7070958

End: 7072760

Strand: Direct

Name: gatA [H]

Synonym: BRADO6814

Alternate gene names: 146343576

Gene position: 7070958-7072760 (Clockwise)

Preceding gene: 146343575

Following gene: 146343587

Centisome position: 94.83

GC content: 69.38

Gene sequence:

>1803_bases
GTGGAGCGCCGCGTGAGCGAAACCGTCGCTGAAATCCTCGCCGCCCATCGCGCCGGCCTGATCACACCCCGAGACACAAT
CGCCCGCTGCTACGAGCGGATTCGCGCCCATGGCGATCCCGCGATCTTCATCAATCTGCGTGACGAGGCCGAGGCGCTGG
CCGAGGCGGACAGCCTGCTCGGGAAAGATCCCGCCGCGCTGCCGCTCTACGGCGTGCCGGTTGCGGTCAAGGACAACATC
GACGTTGCCGGCCTGCCGACCACCGCCGCCTGCCCGGCCTTCGCCTATACGCCCACGCGGGATTCGACCGCGGTGGCGCG
GCTGCGGGCGGCCGGCGCGATCATCATCGGCAAGACCAATCTCGACCAGTTCGCCACCGGCCTCGTCGGCGCGCGGTCGC
CCTATGGCATTCCGAAAAACCCGGTGCGCGGCGATCTGGTGCCGGGCGGCTCGAGCTCCGGCTCGGCGGTCGCCGTGTCG
GCGGGTCTCGTTCCGCTCTCGCTTGGAACGGACACAGCCGGCTCGGGCCGCGTGCCGGCGATGCTGAACAACATTGTCGG
GTTGAAGCCGAGCCTTGGCATGATCTCGACCACCGGACTGGTGCCGGCCTGCCGGACGCTCGACTGCATCTCGGTGTTCT
CGCTGACGGTCGACGATGCGGTCACCGCGCTGTCTGTGATGACGGCGCCCGATCAGCTCGATCCGTTCTCGCGTGACCGG
CCGCTGGCCGCGGTGACGACCTTTCCCAAGGGGGTAAAGCTCGGCGTTCCCCGTCCCGGCCAACTGATCTTCTTCGGCGA
CAAGGCCGCGGAAGCCGCCTATGGCGCGGCCGCGGCGCGCTTCGTCAAGCTCGGCGCCGAGCTGGTCGAATTCGATCTCG
AGCCGTTCTATGAGACGGCGCGGCTGCTCTATGAAGGTCCGTGGGTCGCCGAGCGCTATCTCGTGATCCGCGATCTGCTG
GCGTCGGATCCCGACGCGATTCACCCGGTGACGCGCGAGATCACCATCGGCGGCGCCCGCGGCAGCGCGGCCGACACCTT
CGCGGCGCTGTATCGGCTGCAGGCGCTGCGCAAGGTCGCCGAGCGCACCTTCGGCGAGATCGATGCGATGCTGCTGCCGA
CCGCGCCGACCGCCTACACGACCGCGGAGGTGCTCGCCGATCCGATCCTGCTCAACAGCCGGCTCGGCACCTACACCAAC
TTCGTCAACCTGCTCGATCTGTGCGGCCTCGCAGTGCCGGCCGCGATGCGTGGCGACGGCATTCCGTTCGGCGTCACGCT
GCTGGCGCCCGCCGGCCGCGATGCCGCGCTCGCCAGCCTGGGCCGCGCCTTCCATGCGGACACCGCGCTGCCAGTCGGCG
CCAAGCCGGTGCCGCAGCCGCCGCTCGCGCCGATATCGCCGTCGCTGCATGGCGACGAGATCGCCGTCGTCGTGGTCGGC
GCACATCTCTCCGGCATGGCGCTGAATCACGAGCTGACCAGCCTCGGCGGCCGTATGCTGGAGGCGGCATCGACCGCGCC
GGACTACAAGCTCTACGCGCTGTCGACGACGCCGCCCAAGCCCGGCATGCTCAGGATCGAGGATGGCAAGGGGACCGCGA
TGGTCGTCGAACTATGGGCCCTGTCGGCGGCGAGCTTCGGCAAGTTCGTCAACCTCATCCCGCCGCCGCTGTCGATCGGG
ACGATCAGGCTGGCCGATGGCCGCAGCGCCAAGGGCTTTCTGGTCGAGCCCGCCGCACTCGACGGCGCACGCGACATTTC
GGAATTCGGCGGCTGGCGCGCCTACATGGCGCAGAAGGGCTGA

Upstream 100 bases:

>100_bases
GGCAATGTACACAATGGCACGGCGCTTGCTAACCTCGACGTCAGACCGGCGACGCGGAGCGTAACGATATGGCAAGCGAG
ACTTCGGGCTCACGACAGAC

Downstream 100 bases:

>100_bases
GAACAGCGAAGCCGTAGAGCTTGTGAGCCAATTTGAGTTCAGGGCTGAGTTTGCGACGAGGGGGCTGTCGCCGCGGGATA
GCCGTTGTGGGCGGCTCTGT

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MERRVSETVAEILAAHRAGLITPRDTIARCYERIRAHGDPAIFINLRDEAEALAEADSLLGKDPAALPLYGVPVAVKDNI
DVAGLPTTAACPAFAYTPTRDSTAVARLRAAGAIIIGKTNLDQFATGLVGARSPYGIPKNPVRGDLVPGGSSSGSAVAVS
AGLVPLSLGTDTAGSGRVPAMLNNIVGLKPSLGMISTTGLVPACRTLDCISVFSLTVDDAVTALSVMTAPDQLDPFSRDR
PLAAVTTFPKGVKLGVPRPGQLIFFGDKAAEAAYGAAAARFVKLGAELVEFDLEPFYETARLLYEGPWVAERYLVIRDLL
ASDPDAIHPVTREITIGGARGSAADTFAALYRLQALRKVAERTFGEIDAMLLPTAPTAYTTAEVLADPILLNSRLGTYTN
FVNLLDLCGLAVPAAMRGDGIPFGVTLLAPAGRDAALASLGRAFHADTALPVGAKPVPQPPLAPISPSLHGDEIAVVVVG
AHLSGMALNHELTSLGGRMLEAASTAPDYKLYALSTTPPKPGMLRIEDGKGTAMVVELWALSAASFGKFVNLIPPPLSIG
TIRLADGRSAKGFLVEPAALDGARDISEFGGWRAYMAQKG

Sequences:

>Translated_600_residues
MERRVSETVAEILAAHRAGLITPRDTIARCYERIRAHGDPAIFINLRDEAEALAEADSLLGKDPAALPLYGVPVAVKDNI
DVAGLPTTAACPAFAYTPTRDSTAVARLRAAGAIIIGKTNLDQFATGLVGARSPYGIPKNPVRGDLVPGGSSSGSAVAVS
AGLVPLSLGTDTAGSGRVPAMLNNIVGLKPSLGMISTTGLVPACRTLDCISVFSLTVDDAVTALSVMTAPDQLDPFSRDR
PLAAVTTFPKGVKLGVPRPGQLIFFGDKAAEAAYGAAAARFVKLGAELVEFDLEPFYETARLLYEGPWVAERYLVIRDLL
ASDPDAIHPVTREITIGGARGSAADTFAALYRLQALRKVAERTFGEIDAMLLPTAPTAYTTAEVLADPILLNSRLGTYTN
FVNLLDLCGLAVPAAMRGDGIPFGVTLLAPAGRDAALASLGRAFHADTALPVGAKPVPQPPLAPISPSLHGDEIAVVVVG
AHLSGMALNHELTSLGGRMLEAASTAPDYKLYALSTTPPKPGMLRIEDGKGTAMVVELWALSAASFGKFVNLIPPPLSIG
TIRLADGRSAKGFLVEPAALDGARDISEFGGWRAYMAQKG
>Mature_600_residues
MERRVSETVAEILAAHRAGLITPRDTIARCYERIRAHGDPAIFINLRDEAEALAEADSLLGKDPAALPLYGVPVAVKDNI
DVAGLPTTAACPAFAYTPTRDSTAVARLRAAGAIIIGKTNLDQFATGLVGARSPYGIPKNPVRGDLVPGGSSSGSAVAVS
AGLVPLSLGTDTAGSGRVPAMLNNIVGLKPSLGMISTTGLVPACRTLDCISVFSLTVDDAVTALSVMTAPDQLDPFSRDR
PLAAVTTFPKGVKLGVPRPGQLIFFGDKAAEAAYGAAAARFVKLGAELVEFDLEPFYETARLLYEGPWVAERYLVIRDLL
ASDPDAIHPVTREITIGGARGSAADTFAALYRLQALRKVAERTFGEIDAMLLPTAPTAYTTAEVLADPILLNSRLGTYTN
FVNLLDLCGLAVPAAMRGDGIPFGVTLLAPAGRDAALASLGRAFHADTALPVGAKPVPQPPLAPISPSLHGDEIAVVVVG
AHLSGMALNHELTSLGGRMLEAASTAPDYKLYALSTTPPKPGMLRIEDGKGTAMVVELWALSAASFGKFVNLIPPPLSIG
TIRLADGRSAKGFLVEPAALDGARDISEFGGWRAYMAQKG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=25, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI195972892, Length=254, Percent_Identity=30.3149606299213, Blast_Score=86, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=450, Percent_Identity=28.4444444444444, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71990152, Length=314, Percent_Identity=28.6624203821656, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=206, Percent_Identity=31.0679611650485, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=183, Percent_Identity=32.2404371584699, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=183, Percent_Identity=31.6939890710383, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=550, Percent_Identity=42.7272727272727, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6323950, Length=242, Percent_Identity=31.404958677686, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320448, Length=194, Percent_Identity=33.5051546391753, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648113, Length=271, Percent_Identity=33.210332103321, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24652985, Length=163, Percent_Identity=33.1288343558282, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922090, Length=163, Percent_Identity=33.1288343558282, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652981, Length=163, Percent_Identity=33.1288343558282, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652983, Length=163, Percent_Identity=33.1288343558282, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356731, Length=174, Percent_Identity=30.4597701149425, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550774, Length=289, Percent_Identity=28.719723183391, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24648435, Length=289, Percent_Identity=28.719723183391, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648437, Length=289, Percent_Identity=28.719723183391, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648441, Length=210, Percent_Identity=30, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648439, Length=210, Percent_Identity=30, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 62527; Mature: 62527

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERRVSETVAEILAAHRAGLITPRDTIARCYERIRAHGDPAIFINLRDEAEALAEADSLL
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
GKDPAALPLYGVPVAVKDNIDVAGLPTTAACPAFAYTPTRDSTAVARLRAAGAIIIGKTN
CCCCCCCCEECCCEEEECCCCCCCCCCHHCCCCEEECCCCCCHHHHHHHHCCEEEEECCC
LDQFATGLVGARSPYGIPKNPVRGDLVPGGSSSGSAVAVSAGLVPLSLGTDTAGSGRVPA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCHH
MLNNIVGLKPSLGMISTTGLVPACRTLDCISVFSLTVDDAVTALSVMTAPDQLDPFSRDR
HHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PLAAVTTFPKGVKLGVPRPGQLIFFGDKAAEAAYGAAAARFVKLGAELVEFDLEPFYETA
CEEEEEECCCCCEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RLLYEGPWVAERYLVIRDLLASDPDAIHPVTREITIGGARGSAADTFAALYRLQALRKVA
HHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ERTFGEIDAMLLPTAPTAYTTAEVLADPILLNSRLGTYTNFVNLLDLCGLAVPAAMRGDG
HHHHHCCCEEEECCCCCHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHCHHHCCCC
IPFGVTLLAPAGRDAALASLGRAFHADTALPVGAKPVPQPPLAPISPSLHGDEIAVVVVG
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
AHLSGMALNHELTSLGGRMLEAASTAPDYKLYALSTTPPKPGMLRIEDGKGTAMVVELWA
CCCCCEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCCEEEEEEHH
LSAASFGKFVNLIPPPLSIGTIRLADGRSAKGFLVEPAALDGARDISEFGGWRAYMAQKG
HHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEECCHHCCCHHHHHHHCCEEEECCCCC
>Mature Secondary Structure
MERRVSETVAEILAAHRAGLITPRDTIARCYERIRAHGDPAIFINLRDEAEALAEADSLL
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
GKDPAALPLYGVPVAVKDNIDVAGLPTTAACPAFAYTPTRDSTAVARLRAAGAIIIGKTN
CCCCCCCCEECCCEEEECCCCCCCCCCHHCCCCEEECCCCCCHHHHHHHHCCEEEEECCC
LDQFATGLVGARSPYGIPKNPVRGDLVPGGSSSGSAVAVSAGLVPLSLGTDTAGSGRVPA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCHH
MLNNIVGLKPSLGMISTTGLVPACRTLDCISVFSLTVDDAVTALSVMTAPDQLDPFSRDR
HHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PLAAVTTFPKGVKLGVPRPGQLIFFGDKAAEAAYGAAAARFVKLGAELVEFDLEPFYETA
CEEEEEECCCCCEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RLLYEGPWVAERYLVIRDLLASDPDAIHPVTREITIGGARGSAADTFAALYRLQALRKVA
HHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ERTFGEIDAMLLPTAPTAYTTAEVLADPILLNSRLGTYTNFVNLLDLCGLAVPAAMRGDG
HHHHHCCCEEEECCCCCHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHCHHHCCCC
IPFGVTLLAPAGRDAALASLGRAFHADTALPVGAKPVPQPPLAPISPSLHGDEIAVVVVG
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
AHLSGMALNHELTSLGGRMLEAASTAPDYKLYALSTTPPKPGMLRIEDGKGTAMVVELWA
CCCCCEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCCEEEEEEHH
LSAASFGKFVNLIPPPLSIGTIRLADGRSAKGFLVEPAALDGARDISEFGGWRAYMAQKG
HHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEECCHHCCCHHHHHHHCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA