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The map label for this gene is metZ [H]

Identifier: 146343535

GI number: 146343535

Start: 7028193

End: 7029431

Strand: Direct

Name: metZ [H]

Synonym: BRADO6770

Alternate gene names: 146343535

Gene position: 7028193-7029431 (Clockwise)

Preceding gene: 146343532

Following gene: 146343539

Centisome position: 94.25

GC content: 67.07

Gene sequence:

>1239_bases
ATGTCCAATTCTCCTGCGTCCAACTCTCCTGCGTCGAAGTCCTCCGCGAAGGCCCCGGCGTCGTTCTCTCACTACCGTCC
TGAAACGCGACTGGTGCATTCCGGCGGCCTGAGGTCCGAATTCGGCGAGACCGCCGAGGCGCTCTACCTCACGCAAGGGT
TCGTCTACGCCAGCGCCGAGGAGTGCGAGGCGCGCTTCAAGGGCGAGGATCCCGGCTTTATCTATTCGCGGTTCTCCAAC
CCGACGACCGCGATGTTCGAGCGCCGGATGATCGAGCTCGAAGGCGCCGAAGCCTGCCGCTCGGCCGCGACCGGCATGGC
CGCGGTCACCACCGCGATCCTGGCCCCCTTGCGCGCCGGCGATCACGTCGTCGCGGCCAAGGCGCTGTTCGGCTCCTGCC
GCTACGTCGTCGAGGACCTGCTGCCGCGCTACGGCATCGAGTCGACGCTGGTCGACGGGCTCGATCTCGACCAGTGGCAG
AAGGCGGTCAGGCCAAACACCAAGTCGTTCTTCCTGGAGAGCCCCACCAATCCGACCCTCGACGTCATGGACATTCCGGC
GATCGCCGAGATCGCGCATGCCCATGGCGCGCGGCTGATCGTCGACAACGTGTTCGCGACGCCGATCTGGCAGAGCCCGC
TGGCGCTCGGCGCCGATGTCGTCGTGTACTCCGCGACCAAGCATATCGACGGCCAGGGCCGCTGCCTCGGCGGCGTGATC
CTGTCGTCTGAGGCGTTCATCGCCGAGCACATCCACAACTTCATGCGCCAGACCGGCCCGTCGATCTCGCCATTCAACGC
CTGGGTGCTGCTCAAGGGCCTGGAGACGCTTGCGGTGCGCGTGCGCGCACAGACGGATACGGCGGGAAAGGTCGCCAACT
TCCTCGCCGCGCATCCGAAGATCTCGCGGCTGATCTATCCGGGCCGCGAGGACCATCCGCAGGCGGCGACGGTCAGGAAG
CAGATGCGTGCCGGCTCGACCCTGGTCGGCTTCGAGGTGAAGGGCGGCAAGGCCGCTGCGTTCCGCACGCTCAACGGGCT
CAGGCTCGCGCGGATCTCGAACAATCTCGGCGACTCCAAGACGCTGGTCACGCATCCCGCGACCACCACGCATCAGCGGC
TGACGCCGGAGGCGCGCGCCGAGCTCGGCATCAGCGAGGGCTTCATCCGCTTCTCCGCGGGGCTCGAGCATGCGGATGAT
CTGATCGAGGATCTCGACGCGGCCCTGAAGGCCGCGTAG

Upstream 100 bases:

>100_bases
AACAGGCCGGGGACGATTGTGATCCCGGCCGTGAGGAGTTTTCCCAGGCCGGTTTTTTTGTGCTCATCGCGCTGACAGCG
GTGGTCACGATGGAGGTCAC

Downstream 100 bases:

>100_bases
CGAGTGGCGCATAGGGAGTACCGAGTAGATGATCTGATCCCCTATTCGCCATTCCCAACTCGCTACTCGCCCCCTGATCA
CATCGGCCGATAGGTCAGCA

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSNSPASNSPASKSSAKAPASFSHYRPETRLVHSGGLRSEFGETAEALYLTQGFVYASAEECEARFKGEDPGFIYSRFSN
PTTAMFERRMIELEGAEACRSAATGMAAVTTAILAPLRAGDHVVAAKALFGSCRYVVEDLLPRYGIESTLVDGLDLDQWQ
KAVRPNTKSFFLESPTNPTLDVMDIPAIAEIAHAHGARLIVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRCLGGVI
LSSEAFIAEHIHNFMRQTGPSISPFNAWVLLKGLETLAVRVRAQTDTAGKVANFLAAHPKISRLIYPGREDHPQAATVRK
QMRAGSTLVGFEVKGGKAAAFRTLNGLRLARISNNLGDSKTLVTHPATTTHQRLTPEARAELGISEGFIRFSAGLEHADD
LIEDLDAALKAA

Sequences:

>Translated_412_residues
MSNSPASNSPASKSSAKAPASFSHYRPETRLVHSGGLRSEFGETAEALYLTQGFVYASAEECEARFKGEDPGFIYSRFSN
PTTAMFERRMIELEGAEACRSAATGMAAVTTAILAPLRAGDHVVAAKALFGSCRYVVEDLLPRYGIESTLVDGLDLDQWQ
KAVRPNTKSFFLESPTNPTLDVMDIPAIAEIAHAHGARLIVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRCLGGVI
LSSEAFIAEHIHNFMRQTGPSISPFNAWVLLKGLETLAVRVRAQTDTAGKVANFLAAHPKISRLIYPGREDHPQAATVRK
QMRAGSTLVGFEVKGGKAAAFRTLNGLRLARISNNLGDSKTLVTHPATTTHQRLTPEARAELGISEGFIRFSAGLEHADD
LIEDLDAALKAA
>Mature_411_residues
SNSPASNSPASKSSAKAPASFSHYRPETRLVHSGGLRSEFGETAEALYLTQGFVYASAEECEARFKGEDPGFIYSRFSNP
TTAMFERRMIELEGAEACRSAATGMAAVTTAILAPLRAGDHVVAAKALFGSCRYVVEDLLPRYGIESTLVDGLDLDQWQK
AVRPNTKSFFLESPTNPTLDVMDIPAIAEIAHAHGARLIVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRCLGGVIL
SSEAFIAEHIHNFMRQTGPSISPFNAWVLLKGLETLAVRVRAQTDTAGKVANFLAAHPKISRLIYPGREDHPQAATVRKQ
MRAGSTLVGFEVKGGKAAAFRTLNGLRLARISNNLGDSKTLVTHPATTTHQRLTPEARAELGISEGFIRFSAGLEHADDL
IEDLDAALKAA

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=350, Percent_Identity=37.4285714285714, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI299473759, Length=350, Percent_Identity=34.8571428571429, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI25453487, Length=349, Percent_Identity=31.5186246418338, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1790375, Length=338, Percent_Identity=33.1360946745562, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789383, Length=399, Percent_Identity=27.5689223057644, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560056, Length=354, Percent_Identity=36.7231638418079, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=348, Percent_Identity=36.4942528735632, Blast_Score=224, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI71980708, Length=352, Percent_Identity=32.1022727272727, Blast_Score=200, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=33.7595907928389, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6323333, Length=408, Percent_Identity=30.1470588235294, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=412, Percent_Identity=26.2135922330097, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=295, Percent_Identity=27.4576271186441, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=349, Percent_Identity=29.512893982808, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19922554, Length=350, Percent_Identity=35.7142857142857, Blast_Score=221, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 44314; Mature: 44182

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSPASNSPASKSSAKAPASFSHYRPETRLVHSGGLRSEFGETAEALYLTQGFVYASAE
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCEEEECHH
ECEARFKGEDPGFIYSRFSNPTTAMFERRMIELEGAEACRSAATGMAAVTTAILAPLRAG
HHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
DHVVAAKALFGSCRYVVEDLLPRYGIESTLVDGLDLDQWQKAVRPNTKSFFLESPTNPTL
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCE
DVMDIPAIAEIAHAHGARLIVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRCLGGVI
EEECCCHHHHHHHHCCCEEEEEHHHCCCCCCCCHHCCCCEEEEECHHCCCCCCCEEEEEE
LSSEAFIAEHIHNFMRQTGPSISPFNAWVLLKGLETLAVRVRAQTDTAGKVANFLAAHPK
ECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHCCCC
ISRLIYPGREDHPQAATVRKQMRAGSTLVGFEVKGGKAAAFRTLNGLRLARISNNLGDSK
HHHEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCEEEEEHHCCCCCCC
TLVTHPATTTHQRLTPEARAELGISEGFIRFSAGLEHADDLIEDLDAALKAA
EEEECCCCCCHHHCCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNSPASNSPASKSSAKAPASFSHYRPETRLVHSGGLRSEFGETAEALYLTQGFVYASAE
CCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCEEEECHH
ECEARFKGEDPGFIYSRFSNPTTAMFERRMIELEGAEACRSAATGMAAVTTAILAPLRAG
HHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
DHVVAAKALFGSCRYVVEDLLPRYGIESTLVDGLDLDQWQKAVRPNTKSFFLESPTNPTL
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCE
DVMDIPAIAEIAHAHGARLIVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRCLGGVI
EEECCCHHHHHHHHCCCEEEEEHHHCCCCCCCCHHCCCCEEEEECHHCCCCCCCEEEEEE
LSSEAFIAEHIHNFMRQTGPSISPFNAWVLLKGLETLAVRVRAQTDTAGKVANFLAAHPK
ECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHCCCC
ISRLIYPGREDHPQAATVRKQMRAGSTLVGFEVKGGKAAAFRTLNGLRLARISNNLGDSK
HHHEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCEEEEEHHCCCCCCC
TLVTHPATTTHQRLTPEARAELGISEGFIRFSAGLEHADDLIEDLDAALKAA
EEEECCCCCCHHHCCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]