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The map label for this gene is ogt2 [H]

Identifier: 146343529

GI number: 146343529

Start: 7022931

End: 7023503

Strand: Direct

Name: ogt2 [H]

Synonym: BRADO6764

Alternate gene names: 146343529

Gene position: 7022931-7023503 (Clockwise)

Preceding gene: 146343528

Following gene: 146343530

Centisome position: 94.18

GC content: 65.79

Gene sequence:

>573_bases
ATGGGGCGTGGGTATACGATCTTTGATACGGCCCTCGGCCGTTGCGGCATTGCCTGGAGTGATGCTGGCGTCACTGGCGT
GCAGTTGCCGGAAGCGCGGGAAATCGAGACGCGGCGGCGGCTGTTTCGGCAGTTTCCGGAAGCACGCGAGCAACGCCCGT
GTTCGAATACTGAACTCGCCATTGCGGGCGTCACGGCCATGCTGCGCGGGCAGCCGGTCGACTTCTCCGGCGTCACTCTC
GATCTCAGCGGCGTCACGCCTTTCAACCAGCGCGTTTATGAGGCCATCCGCGCGATTCCGCGCGGCGAGACCCGCAGCGC
GGACGAACTCGCGACCGAGCTGCATGTCCCGAAGGCGCTCACCTCGATCACCCAGGCGCTGATGAAGAATACGTTCGTGC
TGATCGTGCCCTGCCACCGCGTCCGAGACGTAGGCAACGGCCTCGACAAGCCATCGCCGAACAGCGGGATCATCTCCAAG
CGCCGCCTGCTGTCGCTGGAGGGCGCAGGGCCGCAAGCGGGCCTGACCTTGTTCGACGCCCTGTTGGCCGAGGACCGGCG
ACGGGCCTCATAG

Upstream 100 bases:

>100_bases
CCAAATCAGGGCAAACCCGCCCCGGTCGCGCCGCGCTGTTAACAAACTGTTAACAAAGTTGATGCGGGCGAATGATTGTG
GTGTGGGTTGGGGTGGCGTC

Downstream 100 bases:

>100_bases
CCAGCGCGCATCGTGCAAGAATATCCGCACGGTGCCCTCGTCTTCCTCGCGCAGCCACCTAAATCTTCCGGATGACCGCG
ACACTTCTGTTCGATAGTCC

Product: O-6-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MGRGYTIFDTALGRCGIAWSDAGVTGVQLPEAREIETRRRLFRQFPEAREQRPCSNTELAIAGVTAMLRGQPVDFSGVTL
DLSGVTPFNQRVYEAIRAIPRGETRSADELATELHVPKALTSITQALMKNTFVLIVPCHRVRDVGNGLDKPSPNSGIISK
RRLLSLEGAGPQAGLTLFDALLAEDRRRAS

Sequences:

>Translated_190_residues
MGRGYTIFDTALGRCGIAWSDAGVTGVQLPEAREIETRRRLFRQFPEAREQRPCSNTELAIAGVTAMLRGQPVDFSGVTL
DLSGVTPFNQRVYEAIRAIPRGETRSADELATELHVPKALTSITQALMKNTFVLIVPCHRVRDVGNGLDKPSPNSGIISK
RRLLSLEGAGPQAGLTLFDALLAEDRRRAS
>Mature_189_residues
GRGYTIFDTALGRCGIAWSDAGVTGVQLPEAREIETRRRLFRQFPEAREQRPCSNTELAIAGVTAMLRGQPVDFSGVTLD
LSGVTPFNQRVYEAIRAIPRGETRSADELATELHVPKALTSITQALMKNTFVLIVPCHRVRDVGNGLDKPSPNSGIISKR
RLLSLEGAGPQAGLTLFDALLAEDRRRAS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=137, Percent_Identity=32.8467153284672, Blast_Score=68, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115533070, Length=113, Percent_Identity=39.8230088495575, Blast_Score=73, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI115533068, Length=113, Percent_Identity=39.8230088495575, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20730; Mature: 20599

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRGYTIFDTALGRCGIAWSDAGVTGVQLPEAREIETRRRLFRQFPEAREQRPCSNTELA
CCCCCCHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
IAGVTAMLRGQPVDFSGVTLDLSGVTPFNQRVYEAIRAIPRGETRSADELATELHVPKAL
HHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHH
TSITQALMKNTFVLIVPCHRVRDVGNGLDKPSPNSGIISKRRLLSLEGAGPQAGLTLFDA
HHHHHHHHHCCEEEEECHHHHHHHHCCCCCCCCCCCCHHHHHEEEECCCCCHHHHHHHHH
LLAEDRRRAS
HHHHHHHCCC
>Mature Secondary Structure 
GRGYTIFDTALGRCGIAWSDAGVTGVQLPEAREIETRRRLFRQFPEAREQRPCSNTELA
CCCCCHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
IAGVTAMLRGQPVDFSGVTLDLSGVTPFNQRVYEAIRAIPRGETRSADELATELHVPKAL
HHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHH
TSITQALMKNTFVLIVPCHRVRDVGNGLDKPSPNSGIISKRRLLSLEGAGPQAGLTLFDA
HHHHHHHHHCCEEEEECHHHHHHHHCCCCCCCCCCCCHHHHHEEEECCCCCHHHHHHHHH
LLAEDRRRAS
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]