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The map label for this gene is fadA [H]

Identifier: 146343492

GI number: 146343492

Start: 6979704

End: 6980912

Strand: Reverse

Name: fadA [H]

Synonym: BRADO6724

Alternate gene names: 146343492

Gene position: 6980912-6979704 (Counterclockwise)

Preceding gene: 146343493

Following gene: 146343491

Centisome position: 93.62

GC content: 67.91

Gene sequence:

>1209_bases
ATGCCTGAGGCCTTTATCTACGACCACGTACGGACTCCCCGCGGCCGCGGCAAAGCCGACGGATCCCTGCACGAGGTGAC
CGCGCTCGCGCTCGCCACCGTGCCGCTCAAGGCGCTCAAGGACCGCAACAACATCCCCGAGGACGCCGTCGATGACGTCG
TGCTCGGCGTCGTCGATCCCGTCGGCGAAGCCGGCTCGGACATCGCGCGCTTCGCCGCGCTCAACGCCGGCCTCGGCGAG
GCCGTCCCGGGCGTGCAGATCAGCCGCTTCTGCGCCTCCGGCCTCGATGCCGTGAACTTCGCCGCCGCGCAGATCATGAG
CGGCCAGCACGAGCTCGTCATCGGCGGCGGCGCGGAATCGATGAGCCGCGTCGGCATCGGCGCCTCCGGTGGCGCCTGGC
CGATGGATCCGTCGATGGCGGTGCCGTCCTATTTCATGCCGCAAGGCATCTCGGCCGACCTGATCGCCACAAAATACGGC
TTCTCGCGCGACGACGTCGACGCCTATGCGGTGCAGAGCCAGCAGCGCGCCGCCAAGTCCTGGGAAGAGGGCCGCTTCAA
CAAGTCGGTGGTGCCGGTCAAGGACGTCAACGGCCTGACCATCCTCGCCAAGGACGAGCACATGCGCCCGTCCACTACGA
TGCAGTCGCTGGCACAGCTGCAGCCGTCGTTCGCGGCGATGGCGCAGATGGGCGGCTTCGACGCGGTGGCGATCCAGTCG
CATCCGGAGCTCGAGCGCGTCAACTACGTGCATCACGCCGGCAACTCCTCGGGCATCGTCGACGGCGCCGGCGCCGTGCT
GCTCGGCAGCAAGGAGGCCGGCGAGAAGCACGGCATGAAGCCGCGGGCCAAGATCCGCGCCTTCGCCAATATCGGCTCGG
AGCCGGCGATGATGCTGACCGGCCCGGTCGACGTCACCGAGAAGCTGTTCGCGCGCTCCGGCATGACGAAGTCGGACATC
GATCTGTTCGAGCTCAACGAGGCCTTTGCTTCGGTCGTGCTGCGCTACATGCAGGCGTTCGAGATCGACAAGGACAAGAT
CAACGTCAATGGCGGCGCCATCGCGCTGGGTCATCCCTTGGGCGCCACCGGCGCGATGATCCTGGGCACCGTGCTCGACG
AGCTCGAGCGCACCGGCAAGGCGACCGCGCTCGTCACGCTGTGCATCGGCGGCGGCATGGGCACCGCGACCATCATCGAG
CGGGTGTGA

Upstream 100 bases:

>100_bases
GCGGAAGCCTTTTGAGGATCAGACGATGAGCCGGTGCGCGATGCGCGCCGGCTTTGTCGCTCACGTAATTAACGAAATCA
GACCAGATGAGGAGGGCGTG

Downstream 100 bases:

>100_bases
CGGCCGTCATTGCGAGCGAAGCGAAGCAATCCAGAGTCCCACCCGAGCCCTGGATTGCTTCGTCGCTTCGCTCCTCGCAA
TGACGCGTAACTACGGAACG

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MPEAFIYDHVRTPRGRGKADGSLHEVTALALATVPLKALKDRNNIPEDAVDDVVLGVVDPVGEAGSDIARFAALNAGLGE
AVPGVQISRFCASGLDAVNFAAAQIMSGQHELVIGGGAESMSRVGIGASGGAWPMDPSMAVPSYFMPQGISADLIATKYG
FSRDDVDAYAVQSQQRAAKSWEEGRFNKSVVPVKDVNGLTILAKDEHMRPSTTMQSLAQLQPSFAAMAQMGGFDAVAIQS
HPELERVNYVHHAGNSSGIVDGAGAVLLGSKEAGEKHGMKPRAKIRAFANIGSEPAMMLTGPVDVTEKLFARSGMTKSDI
DLFELNEAFASVVLRYMQAFEIDKDKINVNGGAIALGHPLGATGAMILGTVLDELERTGKATALVTLCIGGGMGTATIIE
RV

Sequences:

>Translated_402_residues
MPEAFIYDHVRTPRGRGKADGSLHEVTALALATVPLKALKDRNNIPEDAVDDVVLGVVDPVGEAGSDIARFAALNAGLGE
AVPGVQISRFCASGLDAVNFAAAQIMSGQHELVIGGGAESMSRVGIGASGGAWPMDPSMAVPSYFMPQGISADLIATKYG
FSRDDVDAYAVQSQQRAAKSWEEGRFNKSVVPVKDVNGLTILAKDEHMRPSTTMQSLAQLQPSFAAMAQMGGFDAVAIQS
HPELERVNYVHHAGNSSGIVDGAGAVLLGSKEAGEKHGMKPRAKIRAFANIGSEPAMMLTGPVDVTEKLFARSGMTKSDI
DLFELNEAFASVVLRYMQAFEIDKDKINVNGGAIALGHPLGATGAMILGTVLDELERTGKATALVTLCIGGGMGTATIIE
RV
>Mature_401_residues
PEAFIYDHVRTPRGRGKADGSLHEVTALALATVPLKALKDRNNIPEDAVDDVVLGVVDPVGEAGSDIARFAALNAGLGEA
VPGVQISRFCASGLDAVNFAAAQIMSGQHELVIGGGAESMSRVGIGASGGAWPMDPSMAVPSYFMPQGISADLIATKYGF
SRDDVDAYAVQSQQRAAKSWEEGRFNKSVVPVKDVNGLTILAKDEHMRPSTTMQSLAQLQPSFAAMAQMGGFDAVAIQSH
PELERVNYVHHAGNSSGIVDGAGAVLLGSKEAGEKHGMKPRAKIRAFANIGSEPAMMLTGPVDVTEKLFARSGMTKSDID
LFELNEAFASVVLRYMQAFEIDKDKINVNGGAIALGHPLGATGAMILGTVLDELERTGKATALVTLCIGGGMGTATIIER
V

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=410, Percent_Identity=34.1463414634146, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=33.4170854271357, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI4501853, Length=380, Percent_Identity=36.0526315789474, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI4557237, Length=415, Percent_Identity=30.1204819277108, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI4504327, Length=445, Percent_Identity=30.7865168539326, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI194353979, Length=127, Percent_Identity=40.1574803149606, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=40.1408450704225, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI87082165, Length=417, Percent_Identity=37.1702637889688, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI48994986, Length=411, Percent_Identity=36.9829683698297, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788554, Length=419, Percent_Identity=36.9928400954654, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1788683, Length=427, Percent_Identity=28.8056206088993, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI133906874, Length=412, Percent_Identity=33.495145631068, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI25147385, Length=412, Percent_Identity=31.0679611650485, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17535921, Length=413, Percent_Identity=30.2663438256659, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17551802, Length=449, Percent_Identity=27.8396436525612, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17535917, Length=142, Percent_Identity=31.6901408450704, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322031, Length=359, Percent_Identity=33.4261838440111, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6325229, Length=415, Percent_Identity=30.1204819277108, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=38.1516587677725, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17648125, Length=401, Percent_Identity=34.6633416458853, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24640423, Length=416, Percent_Identity=30.2884615384615, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17137578, Length=447, Percent_Identity=29.082774049217, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42121; Mature: 41989

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAFIYDHVRTPRGRGKADGSLHEVTALALATVPLKALKDRNNIPEDAVDDVVLGVVDP
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
VGEAGSDIARFAALNAGLGEAVPGVQISRFCASGLDAVNFAAAQIMSGQHELVIGGGAES
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCHHH
MSRVGIGASGGAWPMDPSMAVPSYFMPQGISADLIATKYGFSRDDVDAYAVQSQQRAAKS
HHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHH
WEEGRFNKSVVPVKDVNGLTILAKDEHMRPSTTMQSLAQLQPSFAAMAQMGGFDAVAIQS
HHHCCCCCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCEEEECC
HPELERVNYVHHAGNSSGIVDGAGAVLLGSKEAGEKHGMKPRAKIRAFANIGSEPAMMLT
CCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHCCCCHHHHHHHHHHCCCCCCEEEE
GPVDVTEKLFARSGMTKSDIDLFELNEAFASVVLRYMQAFEIDKDKINVNGGAIALGHPL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCC
GATGAMILGTVLDELERTGKATALVTLCIGGGMGTATIIERV
CCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
PEAFIYDHVRTPRGRGKADGSLHEVTALALATVPLKALKDRNNIPEDAVDDVVLGVVDP
CCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
VGEAGSDIARFAALNAGLGEAVPGVQISRFCASGLDAVNFAAAQIMSGQHELVIGGGAES
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCHHH
MSRVGIGASGGAWPMDPSMAVPSYFMPQGISADLIATKYGFSRDDVDAYAVQSQQRAAKS
HHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHH
WEEGRFNKSVVPVKDVNGLTILAKDEHMRPSTTMQSLAQLQPSFAAMAQMGGFDAVAIQS
HHHCCCCCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCEEEECC
HPELERVNYVHHAGNSSGIVDGAGAVLLGSKEAGEKHGMKPRAKIRAFANIGSEPAMMLT
CCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHCCCCHHHHHHHHHHCCCCCCEEEE
GPVDVTEKLFARSGMTKSDIDLFELNEAFASVVLRYMQAFEIDKDKINVNGGAIALGHPL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCC
GATGAMILGTVLDELERTGKATALVTLCIGGGMGTATIIERV
CCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]