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The map label for this gene is gshA

Identifier: 146343486

GI number: 146343486

Start: 6973671

End: 6975041

Strand: Reverse

Name: gshA

Synonym: BRADO6718

Alternate gene names: NA

Gene position: 6975041-6973671 (Counterclockwise)

Preceding gene: 146343487

Following gene: 146343485

Centisome position: 93.54

GC content: 66.08

Gene sequence:

>1371_bases
ATGGCGCGTGACCAGATCGACATGACCCCGCTGCAGTCGCGCGACGAGCTCGTCGCGTGGCTGGAGGCCGGCGTGAAGCC
GCCATCCGCCTTCCGTATCGGCACCGAGCACGAGAAGACCCCGTTCACGCTCGACGGGCACCGGCCGGTGCCGTACGAGG
GCGCCCGCGGCATCGGCGCGCTGCTGGAAGGCATGAAGCTGCTGCTCGGCTGGGAGCCGATCATGGAGCGCGGCAACATC
ATCGGGCTCTATGACGTCACCGGCGGTGGCGCGATCTCGCTGGAGCCGGGCGGCCAGTTCGAACTGTCCGGCGCGCCGGT
CGAGACCGTCCATCAGACCCAGAGCGAGTTGATGGCGCATCTGGCGCAGGTGCGCGAGATCGCCACCCCGCTCGGCATCG
GTTTCCTCGGACTTGGCATGACGCCGTCCTGGACGCGGCAGGAGATCCCGGTGATGCCGAAGGGCCGTTACAAGATCATG
CGCGGCTACATGCCGAAGGTCGGCAGCTACGGCCTGGACATGATGTTCCGGACCTGCACGGTGCAGACCAATCTCGACTT
CTCCTCGGAAGCCGACATGGTCAAGAAGCTGCGCGTCTCCGTCGCGCTGCAGCCGATCGCCACCGCCTTGTTCGCCAATT
CGCCGTTCACGGAAGGAAAGCCGAACGGCTACCTGTCGTTCCGCTCCGAGATCTGGCGCGACACCGACAATCAGCGCTCG
GGCATGATCCCCTGGGCGTTCGAGGACGGCATGGGCTTCGAGCGCTGGGTCGACTACGCGCTCGACGTGCCGATGTACTT
CGTCAAGCGCGAGGAAGACTACATCGACGTGTCGGGCACCTCGTTCCGCGCGTTTTTCGAAGGCAAGAACCCGGCCCTGC
CCGGCGAGCGTCCGAGCCTGTCGGACTGGGCCAATCACCTCTCGACGATCTTTCCGGAGGTACGGCTGAAGCGTTATCTG
GAAATGCGCGGCGCCGACGGCGTGCCGTGGGGCCGGCTGCCGGCGTTGCCGGCGTTCTGGGTCGGCCTGCTGTATGACGA
TGCCAGCCTCGACGCCGCCTGGGAGCTGGTCAAGAACTGGACCGCGTTCGAGCGCCAGAACCTGCGCGATGACGTCCCGC
GTCTCGGCTTCAAGGCGCGCATCCGCAATCGCTATGTGTTCGAGGTCGCCAAGGACTGCCTGACCTTGGCGCATCACGGT
CTGCGCCGCCGCAATCGCGTCGATCATCTCGGCCGCGACGAGAGCCGCTATCTCGAACCGCTCGACCAGATCATCGATTG
CGGCCGCTCGCCCGCCGACGAGATGCTGGAGAAGTTCAATGGCGCCTGGGCCGGCTCGGTCGAGCCAGCCTATGACGAGT
ACGCGTTCTGA

Upstream 100 bases:

>100_bases
ATGAGCCATCCGATCTCATTCACATCCGCGGTCCCCAGGGGTCCGCGGCCCTTCCTGTCACCCTGCGCGACCCTCGACGC
GCCTTTCCACCGAGACTCCC

Downstream 100 bases:

>100_bases
CCGTTTGGAATTGAGCCAAGCATCTTTACGTACGCCCGTTGCTTGATTGGCCGCGGGTGCCGCGGCGGATTCGCTGCGCG
TTCAACCAGGCATCTGACGG

Product: gamma-glutamylcysteine synthetase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MARDQIDMTPLQSRDELVAWLEAGVKPPSAFRIGTEHEKTPFTLDGHRPVPYEGARGIGALLEGMKLLLGWEPIMERGNI
IGLYDVTGGGAISLEPGGQFELSGAPVETVHQTQSELMAHLAQVREIATPLGIGFLGLGMTPSWTRQEIPVMPKGRYKIM
RGYMPKVGSYGLDMMFRTCTVQTNLDFSSEADMVKKLRVSVALQPIATALFANSPFTEGKPNGYLSFRSEIWRDTDNQRS
GMIPWAFEDGMGFERWVDYALDVPMYFVKREEDYIDVSGTSFRAFFEGKNPALPGERPSLSDWANHLSTIFPEVRLKRYL
EMRGADGVPWGRLPALPAFWVGLLYDDASLDAAWELVKNWTAFERQNLRDDVPRLGFKARIRNRYVFEVAKDCLTLAHHG
LRRRNRVDHLGRDESRYLEPLDQIIDCGRSPADEMLEKFNGAWAGSVEPAYDEYAF

Sequences:

>Translated_456_residues
MARDQIDMTPLQSRDELVAWLEAGVKPPSAFRIGTEHEKTPFTLDGHRPVPYEGARGIGALLEGMKLLLGWEPIMERGNI
IGLYDVTGGGAISLEPGGQFELSGAPVETVHQTQSELMAHLAQVREIATPLGIGFLGLGMTPSWTRQEIPVMPKGRYKIM
RGYMPKVGSYGLDMMFRTCTVQTNLDFSSEADMVKKLRVSVALQPIATALFANSPFTEGKPNGYLSFRSEIWRDTDNQRS
GMIPWAFEDGMGFERWVDYALDVPMYFVKREEDYIDVSGTSFRAFFEGKNPALPGERPSLSDWANHLSTIFPEVRLKRYL
EMRGADGVPWGRLPALPAFWVGLLYDDASLDAAWELVKNWTAFERQNLRDDVPRLGFKARIRNRYVFEVAKDCLTLAHHG
LRRRNRVDHLGRDESRYLEPLDQIIDCGRSPADEMLEKFNGAWAGSVEPAYDEYAF
>Mature_455_residues
ARDQIDMTPLQSRDELVAWLEAGVKPPSAFRIGTEHEKTPFTLDGHRPVPYEGARGIGALLEGMKLLLGWEPIMERGNII
GLYDVTGGGAISLEPGGQFELSGAPVETVHQTQSELMAHLAQVREIATPLGIGFLGLGMTPSWTRQEIPVMPKGRYKIMR
GYMPKVGSYGLDMMFRTCTVQTNLDFSSEADMVKKLRVSVALQPIATALFANSPFTEGKPNGYLSFRSEIWRDTDNQRSG
MIPWAFEDGMGFERWVDYALDVPMYFVKREEDYIDVSGTSFRAFFEGKNPALPGERPSLSDWANHLSTIFPEVRLKRYLE
MRGADGVPWGRLPALPAFWVGLLYDDASLDAAWELVKNWTAFERQNLRDDVPRLGFKARIRNRYVFEVAKDCLTLAHHGL
RRRNRVDHLGRDESRYLEPLDQIIDCGRSPADEMLEKFNGAWAGSVEPAYDEYAF

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51483; Mature: 51352

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDQIDMTPLQSRDELVAWLEAGVKPPSAFRIGTEHEKTPFTLDGHRPVPYEGARGIGA
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCEEECCCCCCCCCCCCHHHH
LLEGMKLLLGWEPIMERGNIIGLYDVTGGGAISLEPGGQFELSGAPVETVHQTQSELMAH
HHHHHHHHHCCHHHHHCCCEEEEEEECCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LAQVREIATPLGIGFLGLGMTPSWTRQEIPVMPKGRYKIMRGYMPKVGSYGLDMMFRTCT
HHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHEEE
VQTNLDFSSEADMVKKLRVSVALQPIATALFANSPFTEGKPNGYLSFRSEIWRDTDNQRS
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHCCCCCCCC
GMIPWAFEDGMGFERWVDYALDVPMYFVKREEDYIDVSGTSFRAFFEGKNPALPGERPSL
CCCCEEECCCCCHHHHHHHHHCCCHHEEECCCCEEECCCCEEEEEECCCCCCCCCCCCCH
SDWANHLSTIFPEVRLKRYLEMRGADGVPWGRLPALPAFWVGLLYDDASLDAAWELVKNW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
TAFERQNLRDDVPRLGFKARIRNRYVFEVAKDCLTLAHHGLRRRNRVDHLGRDESRYLEP
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LDQIIDCGRSPADEMLEKFNGAWAGSVEPAYDEYAF
HHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
ARDQIDMTPLQSRDELVAWLEAGVKPPSAFRIGTEHEKTPFTLDGHRPVPYEGARGIGA
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCEEECCCCCCCCCCCCHHHH
LLEGMKLLLGWEPIMERGNIIGLYDVTGGGAISLEPGGQFELSGAPVETVHQTQSELMAH
HHHHHHHHHCCHHHHHCCCEEEEEEECCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LAQVREIATPLGIGFLGLGMTPSWTRQEIPVMPKGRYKIMRGYMPKVGSYGLDMMFRTCT
HHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHEEE
VQTNLDFSSEADMVKKLRVSVALQPIATALFANSPFTEGKPNGYLSFRSEIWRDTDNQRS
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHCCCCCCCC
GMIPWAFEDGMGFERWVDYALDVPMYFVKREEDYIDVSGTSFRAFFEGKNPALPGERPSL
CCCCEEECCCCCHHHHHHHHHCCCHHEEECCCCEEECCCCEEEEEECCCCCCCCCCCCCH
SDWANHLSTIFPEVRLKRYLEMRGADGVPWGRLPALPAFWVGLLYDDASLDAAWELVKNW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
TAFERQNLRDDVPRLGFKARIRNRYVFEVAKDCLTLAHHGLRRRNRVDHLGRDESRYLEP
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LDQIIDCGRSPADEMLEKFNGAWAGSVEPAYDEYAF
HHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA