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The map label for this gene is clpB [H]

Identifier: 146343231

GI number: 146343231

Start: 6702145

End: 6704784

Strand: Reverse

Name: clpB [H]

Synonym: BRADO6450

Alternate gene names: 146343231

Gene position: 6704784-6702145 (Counterclockwise)

Preceding gene: 146343233

Following gene: 146343230

Centisome position: 89.92

GC content: 65.91

Gene sequence:

>2640_bases
ATGAATATCGACAAATATACCGAGCGCGCGCGGGGCTTCATTCAATCGGCGCAGTCTCTGGCGGTGCGCGACGGCCATCA
GCAGTTCTCGCCGCTGCATCTCCTGAAAGTCCTGCTCGACGACAGCGAGGGACTCGCGGGCGGTCTGATCGACCGCGCCG
GCGGCAATTCGCGCGCGATCCTGAAAGCAACCGAGGACGCGCTCGGCAAGATGCCGAAAGTGTCGGGCAGCGGCGCCGGG
CAGATCTACCTGTCCCCGGAATTGGCGCGCGCCTTCGACGCGGCGGAAAAGGCGGCCGAGAAGGCCGGCGACAGTTTCGT
CACCGTCGAGCGGCTGCTGCTCGGGCTGACGCTCGACGCCAAGAGCGAGACCGGAAGCATTCTCGCCAAGGGCGGCGTCA
CCGCGCAGAACCTCAACGCCGCGATCGAGAGCTTGCGCAAGGGGCGCACGGCCGACTCGGCGACCGCCGAGAACGCCTAT
GATGCGCTGAAGAAATATGCCCGCGACCTGACCCAGGCGGCGCGCGACGGCAAGCTCGATCCGGTGATCGGCCGCGACGA
GGAGATTCGCCGCACCATTCAAGTTCTCTCCCGGCGCACCAAGAACAATCCCGTGCTGATCGGCGAGCCCGGCGTCGGCA
AGACCGCGATCGTCGAGGGCCTCGCGCTGCGCATCGTCAATGGTGATGTGCCGGAGAGCCTGAAGGACAAGCGGCTGCTG
TCGCTCGATCTCGGCGCGCTGATTGCGGGTGCCAAATATCGCGGCGAGTTCGAGGAGCGGCTGAAGGCCGTGCTGCAGGA
GGTGACGGCGGCCGAAGGCACCTTCGTGCTGTTCATCGACGAGATGCACACCCTGATCGGCGCCGGCAAGGCTGACGGCG
CGATGGATGCGTCGAACCTGCTCAAGCCTGCGCTCGCGCGCGGCGAGCTGCACTGCATCGGCGCGACCACGCTCGACGAG
TATCAGAAGCATGTCGAGAAGGACGCAGCCCTCGCCCGCCGCTTCCAGCCGGTCTACGTCACCGAGCCCACGGTCGAGGA
CACCATCTCGATCCTGCGCGGCCTGAAGGACAAATACGAGCAGCATCACGGCGTGCGCATCACCGATAGCGCGCTGGTGG
CCTCGGCCACCTTGTCGAACCGCTACATCACCGACCGCTTCCTGCCCGACAAGGCCATCGACCTGATGGACGAGGCGGCG
GCGCGGCTGAAGATGCAGGTCGACTCGAAGCCCGAGGAGCTCGACTCGCTGGATCGCGAGATCATCCGGCTCAAGATCGA
ACAGGAGGCCTTGAAGAAGGAGAGCGATCTCGGCTCCAAGACGAGGCTGCAGGCGCTGGAGAAGGAGCTCGCCGATCTTG
AGGAGAAGTCGGCCTCGCTGACCGCGAAGTGGAGCGCCGAGAAGGACAAGCTGTCGAACGCGCAGAAGCTCAAGAGCGAG
CTGGACGGCTTGCGCATCGAGCTCGCCAACGCGCAGCGCCGCGGCGAGTACCAGAAGGCGGGCGAGCTGGCCTATGGCCG
CATCCCCGAGCTGGAGAAGCGGCTCGCCGACATCGAGGCCAAGGAGACCAGCGGCGAGATGATGGAGGAGGCGGTCACCG
CCAACCACATCGCGCAGGTGGTCTCGCGCTGGACCGGCGTGCCGGTCGACAAGATGCTCGAGGGCGAGAAGGACAAGCTC
TTGCGGATGGAAGACTCGCTAGGCAAACGTGTCGTCGGCCAATTCGAGGCCGTGCATGCGGTCGCGACCGCCGTGCGGCG
CTCGCGCGCGGGCCTGCAGGATCCGAACCGGCCGATGGGCTCGTTCATGTTCTTAGGCCCCACCGGCGTCGGCAAGACCG
AGCTGACCAAGGCGCTCGCCGAGTATCTGTTCAACGACGAGACCGCGATGGTCCGGCTCGACATGTCCGAGTACATGGAG
AAGCACTCGGTGTCGCGGCTGATCGGCGCGCCTCCCGGCTATGTCGGCTATGACGAGGGCGGGGCGCTGACGGAAGCCGT
GCGGCGCCGGCCGTACCAGGTCGTGCTGTTCGACGAGATCGAGAAGGCGCATCCGGACGTGTTCAACGTGCTGCTGCAGG
TGCTCGACGACGGCCGCCTGACCGATGGCCAGGGCCGCACCGTCGACTTCCGCAACACGCTGATCATCATGACTTCGAAT
CTCGGCTCCGAGTTCCTGGTCAATCAGCCCGAGGGCGAGGATACGTCCGAGGTGCGCGAGCTGGTGATGGGCACGGTGCG
CGGGCATTTCCGGCCCGAGTTCCTCAACCGCGTCGACGAGATCATCCTGTTCCACCGGCTGCAGAAGAGCGAGATGGGAC
GGATCGTCGAGATCCAGTTCTCGCGGCTTCAGAAGCTCCTGGAGGAGCGCAAGATCACGCTCTCGCTCGACGGCGACGCG
CGCGACTGGCTCGCCGCCAAGGGCTGGGACCCGGCCTATGGCGCGCGTCCGCTGAAGCGGGTGATCCAGCGCAGCGTGCA
GGATCCGCTCGCCGAGATGATCCTCGCCGGCGACATCAAGGATGGCGACAAGGTCGCCATCTCCTCGGAAGGCAACGTGC
TGACCTTCAACGGCAAGGCGCATCGCACCGCGGAGATCGCGACGTTCGAGGCGCCGATCCCGAAGCGCAAGCTGAACTGA

Upstream 100 bases:

>100_bases
GAGTTGCGGCGTTGAGGAACGTCAACGTGCGCGGTCACAAACCCAATGAAGATGCTGGAACCGTGCCTTCGGGGCGTCAC
AGCAGGCTGAGGGAAACATC

Downstream 100 bases:

>100_bases
TCGGGTAGGCCGAACTCACAGAAGCACGATCGATGTGGTCGTGGATGATCTGGCGAAGCCCCTCCGCTTGCGGAGGGGCT
TTTTCAGTCGCGACCGTCAA

Product: chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 879; Mature: 879

Protein sequence:

>879_residues
MNIDKYTERARGFIQSAQSLAVRDGHQQFSPLHLLKVLLDDSEGLAGGLIDRAGGNSRAILKATEDALGKMPKVSGSGAG
QIYLSPELARAFDAAEKAAEKAGDSFVTVERLLLGLTLDAKSETGSILAKGGVTAQNLNAAIESLRKGRTADSATAENAY
DALKKYARDLTQAARDGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPESLKDKRLL
SLDLGALIAGAKYRGEFEERLKAVLQEVTAAEGTFVLFIDEMHTLIGAGKADGAMDASNLLKPALARGELHCIGATTLDE
YQKHVEKDAALARRFQPVYVTEPTVEDTISILRGLKDKYEQHHGVRITDSALVASATLSNRYITDRFLPDKAIDLMDEAA
ARLKMQVDSKPEELDSLDREIIRLKIEQEALKKESDLGSKTRLQALEKELADLEEKSASLTAKWSAEKDKLSNAQKLKSE
LDGLRIELANAQRRGEYQKAGELAYGRIPELEKRLADIEAKETSGEMMEEAVTANHIAQVVSRWTGVPVDKMLEGEKDKL
LRMEDSLGKRVVGQFEAVHAVATAVRRSRAGLQDPNRPMGSFMFLGPTGVGKTELTKALAEYLFNDETAMVRLDMSEYME
KHSVSRLIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSN
LGSEFLVNQPEGEDTSEVRELVMGTVRGHFRPEFLNRVDEIILFHRLQKSEMGRIVEIQFSRLQKLLEERKITLSLDGDA
RDWLAAKGWDPAYGARPLKRVIQRSVQDPLAEMILAGDIKDGDKVAISSEGNVLTFNGKAHRTAEIATFEAPIPKRKLN

Sequences:

>Translated_879_residues
MNIDKYTERARGFIQSAQSLAVRDGHQQFSPLHLLKVLLDDSEGLAGGLIDRAGGNSRAILKATEDALGKMPKVSGSGAG
QIYLSPELARAFDAAEKAAEKAGDSFVTVERLLLGLTLDAKSETGSILAKGGVTAQNLNAAIESLRKGRTADSATAENAY
DALKKYARDLTQAARDGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPESLKDKRLL
SLDLGALIAGAKYRGEFEERLKAVLQEVTAAEGTFVLFIDEMHTLIGAGKADGAMDASNLLKPALARGELHCIGATTLDE
YQKHVEKDAALARRFQPVYVTEPTVEDTISILRGLKDKYEQHHGVRITDSALVASATLSNRYITDRFLPDKAIDLMDEAA
ARLKMQVDSKPEELDSLDREIIRLKIEQEALKKESDLGSKTRLQALEKELADLEEKSASLTAKWSAEKDKLSNAQKLKSE
LDGLRIELANAQRRGEYQKAGELAYGRIPELEKRLADIEAKETSGEMMEEAVTANHIAQVVSRWTGVPVDKMLEGEKDKL
LRMEDSLGKRVVGQFEAVHAVATAVRRSRAGLQDPNRPMGSFMFLGPTGVGKTELTKALAEYLFNDETAMVRLDMSEYME
KHSVSRLIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSN
LGSEFLVNQPEGEDTSEVRELVMGTVRGHFRPEFLNRVDEIILFHRLQKSEMGRIVEIQFSRLQKLLEERKITLSLDGDA
RDWLAAKGWDPAYGARPLKRVIQRSVQDPLAEMILAGDIKDGDKVAISSEGNVLTFNGKAHRTAEIATFEAPIPKRKLN
>Mature_879_residues
MNIDKYTERARGFIQSAQSLAVRDGHQQFSPLHLLKVLLDDSEGLAGGLIDRAGGNSRAILKATEDALGKMPKVSGSGAG
QIYLSPELARAFDAAEKAAEKAGDSFVTVERLLLGLTLDAKSETGSILAKGGVTAQNLNAAIESLRKGRTADSATAENAY
DALKKYARDLTQAARDGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPESLKDKRLL
SLDLGALIAGAKYRGEFEERLKAVLQEVTAAEGTFVLFIDEMHTLIGAGKADGAMDASNLLKPALARGELHCIGATTLDE
YQKHVEKDAALARRFQPVYVTEPTVEDTISILRGLKDKYEQHHGVRITDSALVASATLSNRYITDRFLPDKAIDLMDEAA
ARLKMQVDSKPEELDSLDREIIRLKIEQEALKKESDLGSKTRLQALEKELADLEEKSASLTAKWSAEKDKLSNAQKLKSE
LDGLRIELANAQRRGEYQKAGELAYGRIPELEKRLADIEAKETSGEMMEEAVTANHIAQVVSRWTGVPVDKMLEGEKDKL
LRMEDSLGKRVVGQFEAVHAVATAVRRSRAGLQDPNRPMGSFMFLGPTGVGKTELTKALAEYLFNDETAMVRLDMSEYME
KHSVSRLIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSN
LGSEFLVNQPEGEDTSEVRELVMGTVRGHFRPEFLNRVDEIILFHRLQKSEMGRIVEIQFSRLQKLLEERKITLSLDGDA
RDWLAAKGWDPAYGARPLKRVIQRSVQDPLAEMILAGDIKDGDKVAISSEGNVLTFNGKAHRTAEIATFEAPIPKRKLN

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=337, Percent_Identity=36.2017804154303, Blast_Score=207, Evalue=5e-53,
Organism=Escherichia coli, GI1788943, Length=844, Percent_Identity=58.6492890995261, Blast_Score=1005, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=337, Percent_Identity=44.8071216617211, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6320464, Length=693, Percent_Identity=57.1428571428571, Blast_Score=809, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=873, Percent_Identity=44.1008018327606, Blast_Score=697, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96765; Mature: 96765

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDKYTERARGFIQSAQSLAVRDGHQQFSPLHLLKVLLDDSEGLAGGLIDRAGGNSRAI
CCCHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
LKATEDALGKMPKVSGSGAGQIYLSPELARAFDAAEKAAEKAGDSFVTVERLLLGLTLDA
EEECHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
KSETGSILAKGGVTAQNLNAAIESLRKGRTADSATAENAYDALKKYARDLTQAARDGKLD
CCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPESLKDKRLL
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHEEEECCCCCCHHHHCHHH
SLDLGALIAGAKYRGEFEERLKAVLQEVTAAEGTFVLFIDEMHTLIGAGKADGAMDASNL
HCCHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCCCCCCCHHHH
LKPALARGELHCIGATTLDEYQKHVEKDAALARRFQPVYVTEPTVEDTISILRGLKDKYE
HHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHH
QHHGVRITDSALVASATLSNRYITDRFLPDKAIDLMDEAAARLKMQVDSKPEELDSLDRE
HHCCEEEECHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IIRLKIEQEALKKESDLGSKTRLQALEKELADLEEKSASLTAKWSAEKDKLSNAQKLKSE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHH
LDGLRIELANAQRRGEYQKAGELAYGRIPELEKRLADIEAKETSGEMMEEAVTANHIAQV
HCCEEEEECCCHHCCCHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
VSRWTGVPVDKMLEGEKDKLLRMEDSLGKRVVGQFEAVHAVATAVRRSRAGLQDPNRPMG
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SFMFLGPTGVGKTELTKALAEYLFNDETAMVRLDMSEYMEKHSVSRLIGAPPGYVGYDEG
CEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSN
CHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECC
LGSEFLVNQPEGEDTSEVRELVMGTVRGHFRPEFLNRVDEIILFHRLQKSEMGRIVEIQF
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHH
SRLQKLLEERKITLSLDGDARDWLAAKGWDPAYGARPLKRVIQRSVQDPLAEMILAGDIK
HHHHHHHHHCCEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DGDKVAISSEGNVLTFNGKAHRTAEIATFEAPIPKRKLN
CCCEEEEECCCCEEEECCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNIDKYTERARGFIQSAQSLAVRDGHQQFSPLHLLKVLLDDSEGLAGGLIDRAGGNSRAI
CCCHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
LKATEDALGKMPKVSGSGAGQIYLSPELARAFDAAEKAAEKAGDSFVTVERLLLGLTLDA
EEECHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
KSETGSILAKGGVTAQNLNAAIESLRKGRTADSATAENAYDALKKYARDLTQAARDGKLD
CCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPESLKDKRLL
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHEEEECCCCCCHHHHCHHH
SLDLGALIAGAKYRGEFEERLKAVLQEVTAAEGTFVLFIDEMHTLIGAGKADGAMDASNL
HCCHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCCCCCCCHHHH
LKPALARGELHCIGATTLDEYQKHVEKDAALARRFQPVYVTEPTVEDTISILRGLKDKYE
HHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHH
QHHGVRITDSALVASATLSNRYITDRFLPDKAIDLMDEAAARLKMQVDSKPEELDSLDRE
HHCCEEEECHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IIRLKIEQEALKKESDLGSKTRLQALEKELADLEEKSASLTAKWSAEKDKLSNAQKLKSE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHH
LDGLRIELANAQRRGEYQKAGELAYGRIPELEKRLADIEAKETSGEMMEEAVTANHIAQV
HCCEEEEECCCHHCCCHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
VSRWTGVPVDKMLEGEKDKLLRMEDSLGKRVVGQFEAVHAVATAVRRSRAGLQDPNRPMG
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SFMFLGPTGVGKTELTKALAEYLFNDETAMVRLDMSEYMEKHSVSRLIGAPPGYVGYDEG
CEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSN
CHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECC
LGSEFLVNQPEGEDTSEVRELVMGTVRGHFRPEFLNRVDEIILFHRLQKSEMGRIVEIQF
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHH
SRLQKLLEERKITLSLDGDARDWLAAKGWDPAYGARPLKRVIQRSVQDPLAEMILAGDIK
HHHHHHHHHCCEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DGDKVAISSEGNVLTFNGKAHRTAEIATFEAPIPKRKLN
CCCEEEEECCCCEEEECCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]