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The map label for this gene is alkJ [H]

Identifier: 146343210

GI number: 146343210

Start: 6673991

End: 6675595

Strand: Direct

Name: alkJ [H]

Synonym: BRADO6420

Alternate gene names: 146343210

Gene position: 6673991-6675595 (Clockwise)

Preceding gene: 146343209

Following gene: 146343213

Centisome position: 89.5

GC content: 66.36

Gene sequence:

>1605_bases
GTGGCGGACGAGGTCGATTTCATCGTCGTGGGTGGCGGCAGTGGCGGAGCGACCGTCGCGGGACGGCTGTCGGAGGATCC
GGGCACATCGGTGATGCTGCTCGATGCCGGCGGGCGCAACGACAATTGGATCGTCACCACCCCCTATATGCTGTTCCTGA
TGGTCGCCGGCCCCGTCAACAACTGGTCGTTCACGACGGTGCCGCAGCCGGGACTCAACGGCCGCATCGGCTATCAGCCG
CGCGGCCGCGGCCTCGGCGGCTCCTCCGCGATCAACGCCATGGTCTACATCCGCGGCCACCGCGCCGACTACGATCAGTG
GGCCGCGCTCGGCAACGCCGGCTGGTCGTATGAGGACGTGCTGCCCTATTTCAAGCGCGCAGAGAACAATGCCGAGTTCA
ACGGCGTATATCACGGCCAGTCGGGCCCGCTGCCGGTCAATCGCCTGCGCACCGACAATCCCGTCCACGAGATCTTCCTG
CAGGCGGCGCGCGAGGCGCAGTTTCCGATCCGCGAGGACTTCAATGCCGAGACCCAGGAAGGTCTCGGCCTGTATCAGGT
CACCCAGCAGAATGGCGAGCGCTGGAGCGCAGCGCGCGCCTACATCCAGCCGCATCTGGGTCAGCGCCGCAATCTGCGCG
TCGAGACCAGGGCCCAGGCCTCGCTGATCCTGTTCGATGGCAAGCGCGCCGTCGGCGTGAAGTATCGCCAGGGCAAGGAG
ATCAAGGAGATCCGCTGCCGCCGCGAGGTGATCCTGGCGTCCGGCGCCTTCCAGACGCCGCAGCTCTTGATGCTCTCCGG
CGTGGGCGATGCGGGCGCCTTGGCAAGGCACGGCATCGCCAGCGTCCATCACCTGCCGGGCGTCGGCCAGAACCTGCAGG
ACCATCCCGACTTCATCTTCGCCTATACGTCCGACAATCCCAACTTCAACTCGCTTTCGCCAAAGGGCATCCGCAGGCTG
CTGGCCGGGATCGGGCAGTATCGGCGCGAGCGCCGCGGCGTGCTGACATCGAACTTCGCCGAGTGCGGCGGCTTCCTGAA
GAGCGCTCCGAATCTCGACATTCCCGACATCCAGCTGCATTTCGGCATGGCCGTCACCGATGATCATGGCCGCAAGCGCC
ACGGCAACGGCTTCTCGTGTCATTTCTGCCTGCTGCGGCCGAAGAGCCGCGGCAGGGTGGCGCTCGGAAGCGCCGATCCG
CTGGCGCCGCCGCTGATCGATCCGAACTTCCTCGGCGAGCAGGACGACGTCGAAACCATGGTCGCAGGCTACAAGACAAC
GCGGCGGCTGATGGAGACGCCGGCGATGCGCAGCCTCGCCACGCGCGACCTGTTCACCGCCGACGTCAGGACCGACGACG
ATATCCGCAGCGTCCTGCGGGCCCGCGTGGACACCGTCTATCACCCGGTCGGCACCTGCAAGATGGGCAGTGCCGATCCG
CTCGCGGTGGTCGATCCGAGCCTCAAGGTGCACGGACTCTCCGGCCTGCGCATCGTCGATGCCTCGGTCATGCCGACTTT
GATCGGCGGCAACACCAACGCCCCGACGATCATGATCGGCGAGAAGGCGGCCGATATGATCAAGGCGGAGCTGCGCGAGT
CCTGA

Upstream 100 bases:

>100_bases
GATTGCGACGCAAGCCAAGCTCGAGATTCCAGGTTCGCCGCTGCGCGGCGCCCCGGAATGACTTCAACAAACAAAAAGAC
ACATCAGGGAGAACACGCGC

Downstream 100 bases:

>100_bases
ATCGTGCCATCACAGACCGCAGCCGCGGCTATCGGCGCTGAGAGCCTCAGCGCCCCATCGGCTTGTAGCCGCCGGAGCCC
ATCGGCTGGTAGGAGGCGGC

Product: glucose-methanol-choline oxidoreductase family protein

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MADEVDFIVVGGGSGGATVAGRLSEDPGTSVMLLDAGGRNDNWIVTTPYMLFLMVAGPVNNWSFTTVPQPGLNGRIGYQP
RGRGLGGSSAINAMVYIRGHRADYDQWAALGNAGWSYEDVLPYFKRAENNAEFNGVYHGQSGPLPVNRLRTDNPVHEIFL
QAAREAQFPIREDFNAETQEGLGLYQVTQQNGERWSAARAYIQPHLGQRRNLRVETRAQASLILFDGKRAVGVKYRQGKE
IKEIRCRREVILASGAFQTPQLLMLSGVGDAGALARHGIASVHHLPGVGQNLQDHPDFIFAYTSDNPNFNSLSPKGIRRL
LAGIGQYRRERRGVLTSNFAECGGFLKSAPNLDIPDIQLHFGMAVTDDHGRKRHGNGFSCHFCLLRPKSRGRVALGSADP
LAPPLIDPNFLGEQDDVETMVAGYKTTRRLMETPAMRSLATRDLFTADVRTDDDIRSVLRARVDTVYHPVGTCKMGSADP
LAVVDPSLKVHGLSGLRIVDASVMPTLIGGNTNAPTIMIGEKAADMIKAELRES

Sequences:

>Translated_534_residues
MADEVDFIVVGGGSGGATVAGRLSEDPGTSVMLLDAGGRNDNWIVTTPYMLFLMVAGPVNNWSFTTVPQPGLNGRIGYQP
RGRGLGGSSAINAMVYIRGHRADYDQWAALGNAGWSYEDVLPYFKRAENNAEFNGVYHGQSGPLPVNRLRTDNPVHEIFL
QAAREAQFPIREDFNAETQEGLGLYQVTQQNGERWSAARAYIQPHLGQRRNLRVETRAQASLILFDGKRAVGVKYRQGKE
IKEIRCRREVILASGAFQTPQLLMLSGVGDAGALARHGIASVHHLPGVGQNLQDHPDFIFAYTSDNPNFNSLSPKGIRRL
LAGIGQYRRERRGVLTSNFAECGGFLKSAPNLDIPDIQLHFGMAVTDDHGRKRHGNGFSCHFCLLRPKSRGRVALGSADP
LAPPLIDPNFLGEQDDVETMVAGYKTTRRLMETPAMRSLATRDLFTADVRTDDDIRSVLRARVDTVYHPVGTCKMGSADP
LAVVDPSLKVHGLSGLRIVDASVMPTLIGGNTNAPTIMIGEKAADMIKAELRES
>Mature_533_residues
ADEVDFIVVGGGSGGATVAGRLSEDPGTSVMLLDAGGRNDNWIVTTPYMLFLMVAGPVNNWSFTTVPQPGLNGRIGYQPR
GRGLGGSSAINAMVYIRGHRADYDQWAALGNAGWSYEDVLPYFKRAENNAEFNGVYHGQSGPLPVNRLRTDNPVHEIFLQ
AAREAQFPIREDFNAETQEGLGLYQVTQQNGERWSAARAYIQPHLGQRRNLRVETRAQASLILFDGKRAVGVKYRQGKEI
KEIRCRREVILASGAFQTPQLLMLSGVGDAGALARHGIASVHHLPGVGQNLQDHPDFIFAYTSDNPNFNSLSPKGIRRLL
AGIGQYRRERRGVLTSNFAECGGFLKSAPNLDIPDIQLHFGMAVTDDHGRKRHGNGFSCHFCLLRPKSRGRVALGSADPL
APPLIDPNFLGEQDDVETMVAGYKTTRRLMETPAMRSLATRDLFTADVRTDDDIRSVLRARVDTVYHPVGTCKMGSADPL
AVVDPSLKVHGLSGLRIVDASVMPTLIGGNTNAPTIMIGEKAADMIKAELRES

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=538, Percent_Identity=42.0074349442379, Blast_Score=423, Evalue=1e-118,
Organism=Escherichia coli, GI1786503, Length=537, Percent_Identity=40.5959031657356, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17532301, Length=543, Percent_Identity=38.3057090239411, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24642055, Length=583, Percent_Identity=37.5643224699828, Blast_Score=344, Evalue=8e-95,
Organism=Drosophila melanogaster, GI24642048, Length=563, Percent_Identity=37.6554174067496, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI45549471, Length=569, Percent_Identity=37.7855887521968, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI45551458, Length=569, Percent_Identity=37.7855887521968, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24642035, Length=579, Percent_Identity=38.860103626943, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24642037, Length=570, Percent_Identity=38.2456140350877, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI17137792, Length=555, Percent_Identity=36.9369369369369, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24642042, Length=578, Percent_Identity=36.3321799307958, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI24650267, Length=574, Percent_Identity=36.2369337979094, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24642039, Length=568, Percent_Identity=36.9718309859155, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24642059, Length=583, Percent_Identity=35.3344768439108, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI18859995, Length=569, Percent_Identity=37.0826010544815, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI18859993, Length=570, Percent_Identity=32.9824561403509, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24642051, Length=576, Percent_Identity=32.6388888888889, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24645930, Length=589, Percent_Identity=31.748726655348, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24642057, Length=585, Percent_Identity=28.5470085470085, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58285; Mature: 58154

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEVDFIVVGGGSGGATVAGRLSEDPGTSVMLLDAGGRNDNWIVTTPYMLFLMVAGPVN
CCCCEEEEEEECCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEECHHHEEHHHCCCCC
NWSFTTVPQPGLNGRIGYQPRGRGLGGSSAINAMVYIRGHRADYDQWAALGNAGWSYEDV
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCHHHH
LPYFKRAENNAEFNGVYHGQSGPLPVNRLRTDNPVHEIFLQAAREAQFPIREDFNAETQE
HHHHHHCCCCCCCCEEEECCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
GLGLYQVTQQNGERWSAARAYIQPHLGQRRNLRVETRAQASLILFDGKRAVGVKYRQGKE
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCEECEEECCCCH
IKEIRCRREVILASGAFQTPQLLMLSGVGDAGALARHGIASVHHLPGVGQNLQDHPDFIF
HHHHHHHHHEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
AYTSDNPNFNSLSPKGIRRLLAGIGQYRRERRGVLTSNFAECGGFLKSAPNLDIPDIQLH
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEE
FGMAVTDDHGRKRHGNGFSCHFCLLRPKSRGRVALGSADPLAPPLIDPNFLGEQDDVETM
EEEEEECCCCCCCCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHH
VAGYKTTRRLMETPAMRSLATRDLFTADVRTDDDIRSVLRARVDTVYHPVGTCKMGSADP
HHHHHHHHHHHHCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LAVVDPSLKVHGLSGLRIVDASVMPTLIGGNTNAPTIMIGEKAADMIKAELRES
EEEECCCEEEECCCCEEEEECCCCCEEECCCCCCCEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
ADEVDFIVVGGGSGGATVAGRLSEDPGTSVMLLDAGGRNDNWIVTTPYMLFLMVAGPVN
CCCEEEEEEECCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEECHHHEEHHHCCCCC
NWSFTTVPQPGLNGRIGYQPRGRGLGGSSAINAMVYIRGHRADYDQWAALGNAGWSYEDV
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCHHHH
LPYFKRAENNAEFNGVYHGQSGPLPVNRLRTDNPVHEIFLQAAREAQFPIREDFNAETQE
HHHHHHCCCCCCCCEEEECCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
GLGLYQVTQQNGERWSAARAYIQPHLGQRRNLRVETRAQASLILFDGKRAVGVKYRQGKE
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCEECEEECCCCH
IKEIRCRREVILASGAFQTPQLLMLSGVGDAGALARHGIASVHHLPGVGQNLQDHPDFIF
HHHHHHHHHEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
AYTSDNPNFNSLSPKGIRRLLAGIGQYRRERRGVLTSNFAECGGFLKSAPNLDIPDIQLH
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEE
FGMAVTDDHGRKRHGNGFSCHFCLLRPKSRGRVALGSADPLAPPLIDPNFLGEQDDVETM
EEEEEECCCCCCCCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHH
VAGYKTTRRLMETPAMRSLATRDLFTADVRTDDDIRSVLRARVDTVYHPVGTCKMGSADP
HHHHHHHHHHHHCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LAVVDPSLKVHGLSGLRIVDASVMPTLIGGNTNAPTIMIGEKAADMIKAELRES
EEEECCCEEEECCCCEEEEECCCCCEEECCCCCCCEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]