Click here to switch to the map view.

The map label for this gene is pinE [H]

Identifier: 146343206

GI number: 146343206

Start: 6668551

End: 6669114

Strand: Direct

Name: pinE [H]

Synonym: BRADO6414

Alternate gene names: 146343206

Gene position: 6668551-6669114 (Clockwise)

Preceding gene: 146343203

Following gene: 146343207

Centisome position: 89.43

GC content: 61.7

Gene sequence:

>564_bases
ATGACCATCTTCGGTTACGCCCGCGTCAGTACGTCCGGCCAGGAGTTGGATATTCAGATCGCGCAGCTCGAAGCGGCGGG
CTGCGAGCGCATCCTTTGCGAGAAGCAGAGCGGGGTTAACGATCAACGCCGAGAACTGGCGCGGTTGCTGCGCCGTCTCC
GCAAGGGCGATACCGTCATCGTCCCTGCGCTCGATCGGCTTACCCGTGGCGGCCCCTTCAAGATGCTGAGCGTGCTCGCC
GACATCACCTCTCGCGGCGCCAGCTACAGATCTCTTGCTGAGCCCTGGGCAGATACCACGCATGAGCTCGGCGAAGTGCT
GGCGGCGCTGGTGGGCTACATTGCCCGAAAGACGCGCGAGGATATTCTTCGCCGCACAAGTGCAGGCCGCGAACGGGCGC
TCAAGAACGGAGTCCGGTTCGGTCGAAAGCCGAAGCTTACGGCCGAGCAACGGTCATCTGCTCTTGCCCGTAGGCGTTCC
GGTGAGCCGCAGAAGCTCATTGCGCGTGACTATGACGTGAGCTGTTCGACAATTTCGAGATTGAAAGATCCTGAGCGCGG
ATGA

Upstream 100 bases:

>100_bases
TTGATCAGACTCAGGAAAGCGGCGATTTGTTGCCCCCTCGCCTATCCATTGCCGATGGCCTTGCAAAATTCAGGAGTTTT
GCAACAGGTGGCCGGCGGCA

Downstream 100 bases:

>100_bases
TGCTTAAATAATGTGCACCGGATCAGTACCAACACTTGTGAGGCTGACGAGCTGAGCGCATGTGTCGTCCTATGGACCTA
AAAAATCTCCAAACAGCCTA

Product: putative DNA-invertase from bacteriophage

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTIFGYARVSTSGQELDIQIAQLEAAGCERILCEKQSGVNDQRRELARLLRRLRKGDTVIVPALDRLTRGGPFKMLSVLA
DITSRGASYRSLAEPWADTTHELGEVLAALVGYIARKTREDILRRTSAGRERALKNGVRFGRKPKLTAEQRSSALARRRS
GEPQKLIARDYDVSCSTISRLKDPERG

Sequences:

>Translated_187_residues
MTIFGYARVSTSGQELDIQIAQLEAAGCERILCEKQSGVNDQRRELARLLRRLRKGDTVIVPALDRLTRGGPFKMLSVLA
DITSRGASYRSLAEPWADTTHELGEVLAALVGYIARKTREDILRRTSAGRERALKNGVRFGRKPKLTAEQRSSALARRRS
GEPQKLIARDYDVSCSTISRLKDPERG
>Mature_186_residues
TIFGYARVSTSGQELDIQIAQLEAAGCERILCEKQSGVNDQRRELARLLRRLRKGDTVIVPALDRLTRGGPFKMLSVLAD
ITSRGASYRSLAEPWADTTHELGEVLAALVGYIARKTREDILRRTSAGRERALKNGVRFGRKPKLTAEQRSSALARRRSG
EPQKLIARDYDVSCSTISRLKDPERG

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=179, Percent_Identity=40.7821229050279, Blast_Score=110, Evalue=4e-26,
Organism=Escherichia coli, GI1787827, Length=186, Percent_Identity=34.4086021505376, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1787638, Length=186, Percent_Identity=34.4086021505376, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20853; Mature: 20722

Theoretical pI: Translated: 10.88; Mature: 10.88

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIFGYARVSTSGQELDIQIAQLEAAGCERILCEKQSGVNDQRRELARLLRRLRKGDTVI
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEE
VPALDRLTRGGPFKMLSVLADITSRGASYRSLAEPWADTTHELGEVLAALVGYIARKTRE
EECHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DILRRTSAGRERALKNGVRFGRKPKLTAEQRSSALARRRSGEPQKLIARDYDVSCSTISR
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHH
LKDPERG
CCCCCCC
>Mature Secondary Structure 
TIFGYARVSTSGQELDIQIAQLEAAGCERILCEKQSGVNDQRRELARLLRRLRKGDTVI
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEE
VPALDRLTRGGPFKMLSVLADITSRGASYRSLAEPWADTTHELGEVLAALVGYIARKTRE
EECHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DILRRTSAGRERALKNGVRFGRKPKLTAEQRSSALARRRSGEPQKLIARDYDVSCSTISR
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHH
LKDPERG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]