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The map label for this gene is tnpR [H]

Identifier: 146343193

GI number: 146343193

Start: 6654768

End: 6655373

Strand: Reverse

Name: tnpR [H]

Synonym: BRADO6397

Alternate gene names: 146343193

Gene position: 6655373-6654768 (Counterclockwise)

Preceding gene: 146343195

Following gene: 146343192

Centisome position: 89.25

GC content: 61.06

Gene sequence:

>606_bases
GTGACGGGACAGGTTGTCGGTTATGTACGGGTGAGCACACTCGACCAGAAGACCGATCGGCAGCTTGTTGGCGTCGACCT
TCATCGCACCTTCACCGACCACGCCTCCGGTAAAGATACCGATCGGCCCCAGCTTGCCCAGATGATCGCCTATGTGCGCG
AGGGCGACACGGTGGTGGTCCATTCAATGGATCGCTTGGCGCGCAATCTTGAAGATCTGCGGGCCTTGGTGCGGACGCTC
ACCGTGAAGGGCGTCCGCGTGCAGTTCGTGAAGGAGAGCCTCACGTTTACGGCTGAGGCCTCGCCTATGTCCCAGCTGTT
GCTCAACGTCATGGGCTCCTTTGCCGAGTTTGAGCGGGCGCTCATCCGCGAGCGCCAGCGGGAAGGCATCGCAGTGGCCA
AGCGCAAGGGCGTTTATAAAGGGCGTAAGCGCGTTCTCACGCCTGATCAAGTCGCTGAGATCGTCAAGCAGGTCGACGCC
AGGGCGAAGAAGGCCAAGCTTGCCCGCGACTTCGGCGTCAGTCGCGAGACTATTCACCGCTACCTACGGGCTGCGGCTTC
GGCCGCTGCCGCCGAACAAGAACAACAAGAGGAGGAAACTGCATGA

Upstream 100 bases:

>100_bases
CTGGCCCAAAAAAGTGTGTCACATAAATCGATGCTGGCTATTGTTGACACGTGAGTCATCACCTCTCAGAATCCACGCCA
CAGAATGAAAGCATGGGAAG

Downstream 100 bases:

>100_bases
CCGCCATCATGCTCACCCGCATCAATGACAGCCGCAACATGGCGCGGTTCTACAAGCTGGACGTCCAGCCGACGCTCTTC
GGCGAGTGGTCGTTTGTGAA

Product: transposon Tn21 resolvase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MTGQVVGYVRVSTLDQKTDRQLVGVDLHRTFTDHASGKDTDRPQLAQMIAYVREGDTVVVHSMDRLARNLEDLRALVRTL
TVKGVRVQFVKESLTFTAEASPMSQLLLNVMGSFAEFERALIRERQREGIAVAKRKGVYKGRKRVLTPDQVAEIVKQVDA
RAKKAKLARDFGVSRETIHRYLRAAASAAAAEQEQQEEETA

Sequences:

>Translated_201_residues
MTGQVVGYVRVSTLDQKTDRQLVGVDLHRTFTDHASGKDTDRPQLAQMIAYVREGDTVVVHSMDRLARNLEDLRALVRTL
TVKGVRVQFVKESLTFTAEASPMSQLLLNVMGSFAEFERALIRERQREGIAVAKRKGVYKGRKRVLTPDQVAEIVKQVDA
RAKKAKLARDFGVSRETIHRYLRAAASAAAAEQEQQEEETA
>Mature_200_residues
TGQVVGYVRVSTLDQKTDRQLVGVDLHRTFTDHASGKDTDRPQLAQMIAYVREGDTVVVHSMDRLARNLEDLRALVRTLT
VKGVRVQFVKESLTFTAEASPMSQLLLNVMGSFAEFERALIRERQREGIAVAKRKGVYKGRKRVLTPDQVAEIVKQVDAR
AKKAKLARDFGVSRETIHRYLRAAASAAAAEQEQQEEETA

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=186, Percent_Identity=34.9462365591398, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787827, Length=186, Percent_Identity=32.258064516129, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787638, Length=186, Percent_Identity=31.7204301075269, Blast_Score=94, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22538; Mature: 22407

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQVVGYVRVSTLDQKTDRQLVGVDLHRTFTDHASGKDTDRPQLAQMIAYVREGDTVVV
CCCCEEEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEE
HSMDRLARNLEDLRALVRTLTVKGVRVQFVKESLTFTAEASPMSQLLLNVMGSFAEFERA
ECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LIRERQREGIAVAKRKGVYKGRKRVLTPDQVAEIVKQVDARAKKAKLARDFGVSRETIHR
HHHHHHHCCHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YLRAAASAAAAEQEQQEEETA
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGQVVGYVRVSTLDQKTDRQLVGVDLHRTFTDHASGKDTDRPQLAQMIAYVREGDTVVV
CCCEEEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEE
HSMDRLARNLEDLRALVRTLTVKGVRVQFVKESLTFTAEASPMSQLLLNVMGSFAEFERA
ECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LIRERQREGIAVAKRKGVYKGRKRVLTPDQVAEIVKQVDARAKKAKLARDFGVSRETIHR
HHHHHHHCCHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YLRAAASAAAAEQEQQEEETA
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2992807; 6312271; 3029727 [H]