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The map label for this gene is pitA [H]

Identifier: 146343161

GI number: 146343161

Start: 6617481

End: 6619109

Strand: Reverse

Name: pitA [H]

Synonym: BRADO6359

Alternate gene names: 146343161

Gene position: 6619109-6617481 (Counterclockwise)

Preceding gene: 146343169

Following gene: 146343160

Centisome position: 88.77

GC content: 64.76

Gene sequence:

>1629_bases
ATGGCCGAAATCACAGTCAACCAAGCCGTCGGCGACGAGCGGCCGATTCAGCCGGCCGGACGGCCGAATCTCGACAAGGG
GTTCAACCCGCTGACGATGATCCTCTTCCTCGGCATCCTCGCCGCCGGCCTGATGTTCGTGGCCTACAGCATCTATGTCG
ATGTCGACGCCACCGGCGTGAAGGTGACGACCTATCTGCCCTACATGCTGCTGTTCGTCGCGCTGCTGATCGCGCTCGGC
TTCGAATTCGTCAACGGCTTCCACGACACCGCCAACGCGGTGGCGACGGTGATCTATACGCACTCGCTGCCGGCGGAATT
CGCCGTGATGTGGTCCGGCTTCTTCAACTTCCTCGGCGTGCTGCTCTCCTCCGGCGCGGTCGCCTTTGGCATCGTCTCGC
TGCTGCCGGTCGAGCTGATCCTGCAGGTCGGCTCCAGCGCCGGCTTCGCGATGGTGTTCGCGCTGCTGATCGCCGCGATC
ATCTGGAATCTCGGCACCTGGTATTTCGGCCTGCCGGCCTCGTCCTCGCACACGCTGATCGGCTCGATCATCGGCGTCGG
CGTCGCCAATGCGCTGCTGCGCGGCCGCGACGGCACCTCCGGCGTGGACTGGAGCAAGGCGACCGAGATCGGCTACGCGC
TCCTGCTGTCGCCGCTGGTCGGCTTCATCTGCGCCGCCGCGCTGCTCTACATCCTCAAATTCCTGGTCCGCAACCCCGCG
CTCTACGCCGCGCCGGAAGGCGTCAAGGCGCCGCCGTGGTGGATCCGTGGCCTCTTGATCCTGACTTGCACCGGCGTGTC
CTTCGCGCATGGTTCGAACGACGGCCAGAAGGGCATGGGCCTGATCATGCTGATCCTGATCGGCACCGTGCCGACGGCCT
ATGCGCTGAACCGCACGCTGCCGGAATCGCACATCGCGAAATTCCAGGAGACCTCACAGGCGGCCTCCAAGGTGATCGCC
GCCAAGGCCGCCGGCTACAACGTGATCGGCGACCCGCGTCCGGCCGTCACGCTCTATGTTTCGCAGCACAAGATCAACGA
GGGCACCTATCCCTCGCTCGCCGTGCTTGTGAAGGACGTCGGCGATCAGGTGCGCAACTATGGCTCGCTCAACAAGGTGC
CGGCCGAGGCGGTCGGCAATACTCGTAACGACATGTACCTGACCTCGGAGGCGATCCGCTTCCTGATGAAGGACAAGGAG
AGCGATCTCAACAAGGACGAGATCGCGACCCTGAACGCCTACAAGGCCTCGCTCGACAGCGCCACCAAGTTCATCCCGAC
ATGGGTCAAGATCGCTGTCGCGATCGCGCTCGGCCTCGGCACGATGATCGGCTGGAAGCGCATCGTGGTGACGGTCGGCG
AGAAGATCGGCAAGTCGCATCTGACCTATGCGCAGGGTGCATCGGCCGAGCTGGTCGCCGCCGCCACGATCGGCGCTGCA
GACGTGTTCGGTCTGCCGGTGTCGACCACCCACGTGCTGTCCTCGGGCGTCGCCGGCACGATGGCGGCCAACGGCTCCGG
GCTGCAGATGTCGACGGTCCGCAACCTGCTGATGGCCTGGGTGCTGACCTTGCCAGCCGCGATCCTGCTGTCGGGCTCGC
TCTACGTGATCTTCAGCCGGATCTTCTGA

Upstream 100 bases:

>100_bases
ACAGCTGTTTTCGGCAGGGGTGCGAGCGCTTGTCATCTGCGCTTCGCATCTGCGCTCCAAAATCAGATCTCCGAGTACTG
GGGTCTACGGGGAGAGCCAC

Downstream 100 bases:

>100_bases
CGATGCGCGCTGACCGGGCGGTCCTCGCAGCCGTGACGGCGCCGCCTCTGGAGGGGCTGGTGTGGGCCTTCCGCTTCACG
GCGGACGGCACGCCCCAGCC

Product: inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MAEITVNQAVGDERPIQPAGRPNLDKGFNPLTMILFLGILAAGLMFVAYSIYVDVDATGVKVTTYLPYMLLFVALLIALG
FEFVNGFHDTANAVATVIYTHSLPAEFAVMWSGFFNFLGVLLSSGAVAFGIVSLLPVELILQVGSSAGFAMVFALLIAAI
IWNLGTWYFGLPASSSHTLIGSIIGVGVANALLRGRDGTSGVDWSKATEIGYALLLSPLVGFICAAALLYILKFLVRNPA
LYAAPEGVKAPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRTLPESHIAKFQETSQAASKVIA
AKAAGYNVIGDPRPAVTLYVSQHKINEGTYPSLAVLVKDVGDQVRNYGSLNKVPAEAVGNTRNDMYLTSEAIRFLMKDKE
SDLNKDEIATLNAYKASLDSATKFIPTWVKIAVAIALGLGTMIGWKRIVVTVGEKIGKSHLTYAQGASAELVAAATIGAA
DVFGLPVSTTHVLSSGVAGTMAANGSGLQMSTVRNLLMAWVLTLPAAILLSGSLYVIFSRIF

Sequences:

>Translated_542_residues
MAEITVNQAVGDERPIQPAGRPNLDKGFNPLTMILFLGILAAGLMFVAYSIYVDVDATGVKVTTYLPYMLLFVALLIALG
FEFVNGFHDTANAVATVIYTHSLPAEFAVMWSGFFNFLGVLLSSGAVAFGIVSLLPVELILQVGSSAGFAMVFALLIAAI
IWNLGTWYFGLPASSSHTLIGSIIGVGVANALLRGRDGTSGVDWSKATEIGYALLLSPLVGFICAAALLYILKFLVRNPA
LYAAPEGVKAPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRTLPESHIAKFQETSQAASKVIA
AKAAGYNVIGDPRPAVTLYVSQHKINEGTYPSLAVLVKDVGDQVRNYGSLNKVPAEAVGNTRNDMYLTSEAIRFLMKDKE
SDLNKDEIATLNAYKASLDSATKFIPTWVKIAVAIALGLGTMIGWKRIVVTVGEKIGKSHLTYAQGASAELVAAATIGAA
DVFGLPVSTTHVLSSGVAGTMAANGSGLQMSTVRNLLMAWVLTLPAAILLSGSLYVIFSRIF
>Mature_541_residues
AEITVNQAVGDERPIQPAGRPNLDKGFNPLTMILFLGILAAGLMFVAYSIYVDVDATGVKVTTYLPYMLLFVALLIALGF
EFVNGFHDTANAVATVIYTHSLPAEFAVMWSGFFNFLGVLLSSGAVAFGIVSLLPVELILQVGSSAGFAMVFALLIAAII
WNLGTWYFGLPASSSHTLIGSIIGVGVANALLRGRDGTSGVDWSKATEIGYALLLSPLVGFICAAALLYILKFLVRNPAL
YAAPEGVKAPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRTLPESHIAKFQETSQAASKVIAA
KAAGYNVIGDPRPAVTLYVSQHKINEGTYPSLAVLVKDVGDQVRNYGSLNKVPAEAVGNTRNDMYLTSEAIRFLMKDKES
DLNKDEIATLNAYKASLDSATKFIPTWVKIAVAIALGLGTMIGWKRIVVTVGEKIGKSHLTYAQGASAELVAAATIGAAD
VFGLPVSTTHVLSSGVAGTMAANGSGLQMSTVRNLLMAWVLTLPAAILLSGSLYVIFSRIF

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=483, Percent_Identity=45.1345755693582, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1789360, Length=482, Percent_Identity=42.5311203319502, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25146401, Length=385, Percent_Identity=23.6363636363636, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 57345; Mature: 57214

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITVNQAVGDERPIQPAGRPNLDKGFNPLTMILFLGILAAGLMFVAYSIYVDVDATGV
CCEEEECHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCE
KVTTYLPYMLLFVALLIALGFEFVNGFHDTANAVATVIYTHSLPAEFAVMWSGFFNFLGV
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LLSSGAVAFGIVSLLPVELILQVGSSAGFAMVFALLIAAIIWNLGTWYFGLPASSSHTLI
HHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHH
GSIIGVGVANALLRGRDGTSGVDWSKATEIGYALLLSPLVGFICAAALLYILKFLVRNPA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LYAAPEGVKAPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRTL
EEECCCCCCCCHHHHHHHHHHEECCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHCCC
PESHIAKFQETSQAASKVIAAKAAGYNVIGDPRPAVTLYVSQHKINEGTYPSLAVLVKDV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHH
GDQVRNYGSLNKVPAEAVGNTRNDMYLTSEAIRFLMKDKESDLNKDEIATLNAYKASLDS
HHHHHCCCCCCCCCHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ATKFIPTWVKIAVAIALGLGTMIGWKRIVVTVGEKIGKSHLTYAQGASAELVAAATIGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCEEEHHHHCHH
DVFGLPVSTTHVLSSGVAGTMAANGSGLQMSTVRNLLMAWVLTLPAAILLSGSLYVIFSR
HHCCCCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IF
CC
>Mature Secondary Structure 
AEITVNQAVGDERPIQPAGRPNLDKGFNPLTMILFLGILAAGLMFVAYSIYVDVDATGV
CEEEECHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCE
KVTTYLPYMLLFVALLIALGFEFVNGFHDTANAVATVIYTHSLPAEFAVMWSGFFNFLGV
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LLSSGAVAFGIVSLLPVELILQVGSSAGFAMVFALLIAAIIWNLGTWYFGLPASSSHTLI
HHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHH
GSIIGVGVANALLRGRDGTSGVDWSKATEIGYALLLSPLVGFICAAALLYILKFLVRNPA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LYAAPEGVKAPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRTL
EEECCCCCCCCHHHHHHHHHHEECCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHCCC
PESHIAKFQETSQAASKVIAAKAAGYNVIGDPRPAVTLYVSQHKINEGTYPSLAVLVKDV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHH
GDQVRNYGSLNKVPAEAVGNTRNDMYLTSEAIRFLMKDKESDLNKDEIATLNAYKASLDS
HHHHHCCCCCCCCCHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ATKFIPTWVKIAVAIALGLGTMIGWKRIVVTVGEKIGKSHLTYAQGASAELVAAATIGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCEEEHHHHCHH
DVFGLPVSTTHVLSSGVAGTMAANGSGLQMSTVRNLLMAWVLTLPAAILLSGSLYVIFSR
HHCCCCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]