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The map label for this gene is potD [C]

Identifier: 146342994

GI number: 146342994

Start: 6408122

End: 6409237

Strand: Direct

Name: potD [C]

Synonym: BRADO6178

Alternate gene names: 146342994

Gene position: 6408122-6409237 (Clockwise)

Preceding gene: 146342993

Following gene: 146342995

Centisome position: 85.94

GC content: 66.04

Gene sequence:

>1116_bases
ATGTTGTCTCTGCGGACCACCCCCACCAACGCAATGATGTCGAACAGGACGGATCGGGCCGCATCGCGCGCGCCGATACG
CGTCGCGACCGCGATTCTGGTCGCCGCGGCCGCTCTGATCAGCGTCGCGGCGTCAGCGCAGGCGCGCGACCTCACCGTCG
TGTCGTGGGGCGGCAACTACCAGGACGCGCAGCGCAAGATCTATTTCCAGCCGTTCGCCGCCAAGCTCGGCGCCACCGTG
CTCGACGAGAGCTGGGATGGCGGCTACGGCGTGCTGCAGGCCAAGGTGAAGGCGGGCGTGCCGAACTGGGATGCGGTGCA
GGTCGAGGCCGAGGAGCTCGAGCTCGGCTGCAGCGACGGCATCTATGAGAAGATCGACTGGAGCAAGCTCGGCGGCAAGG
AGGGCTTCATTCCGGCCGGCGTCAGCGATTGCGGCGTCGGCGCGATCGTCTGGTCCACCGGCATGGCGTTCGACAGCGAC
AAGATCAAGGATGGGCCGAAGACCTGGGCGGACTTCTGGGACACGACGAAGTTTCCCGGCAAGCGCGGCCTGCGCAAGGG
CGCGAAATACACGCTGGAATTCGCGCTGATGGCCGACGGCGTGCCGGCCGCCGACGTCTACAAGGTGCTGAAGACCAAGG
AGGGTGTCGAGCGCGCCTTCAAGAAGCTCGACGCGCTCAAGGCCGACATCGTCTGGTGGGAAGCCGGCGCGCAGCCGCTG
CAGCTGCTGTCGTCGGGCCAGGTTGCAATGACCTCGGTCTATAACGGCCGCATCACCGCCATCAACCGCACCGAGGGCAA
GCATTTCGGCTTCGTTTTCCCGGGCAGCATCTATGCGGTCGACAGCTGGGTCGTGCTGAAGGGCTCGCCGAACAAGGACG
CCGCGATGGCCTTCATCGCCTTTGCGAGCCAGGCCGACAACCAGGCCAAGCTGCCGGAATACATCGCCTACGGCCTGCCG
AACATCGCCGCCGCCAAGCTGGTGCCGGAGAAGTACGCCGCGGAGCTGCCGACCACGCCGGACAATCTCAAGCAGGCGAT
CGCGCTCGACGTCGGCTTCTGGACCGACAATTCCGAAGAGCTGTCGAAGCGCTTCAACGCCTGGCTGGCGCGCTGA

Upstream 100 bases:

>100_bases
CCAAACTAGCATCAGGATCAGGTCAAAACTCGCGCTTGCCAGAGCTTTGGACAGCCGGTAGCTTTTTTGTACCATTCAAA
AGCGCGACGGATCATCGAGC

Downstream 100 bases:

>100_bases
GGCCATCGCCGATCGGGCGCGCGATCCTTCGCGCGTCCGCCTTCCTCGTGCATGAGTTCCGGCGCAATCGGCGCTGAAAA
GCCAGCGTTCATGGACAGCC

Product: spermidine/putrescine ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLSLRTTPTNAMMSNRTDRAASRAPIRVATAILVAAAALISVAASAQARDLTVVSWGGNYQDAQRKIYFQPFAAKLGATV
LDESWDGGYGVLQAKVKAGVPNWDAVQVEAEELELGCSDGIYEKIDWSKLGGKEGFIPAGVSDCGVGAIVWSTGMAFDSD
KIKDGPKTWADFWDTTKFPGKRGLRKGAKYTLEFALMADGVPAADVYKVLKTKEGVERAFKKLDALKADIVWWEAGAQPL
QLLSSGQVAMTSVYNGRITAINRTEGKHFGFVFPGSIYAVDSWVVLKGSPNKDAAMAFIAFASQADNQAKLPEYIAYGLP
NIAAAKLVPEKYAAELPTTPDNLKQAIALDVGFWTDNSEELSKRFNAWLAR

Sequences:

>Translated_371_residues
MLSLRTTPTNAMMSNRTDRAASRAPIRVATAILVAAAALISVAASAQARDLTVVSWGGNYQDAQRKIYFQPFAAKLGATV
LDESWDGGYGVLQAKVKAGVPNWDAVQVEAEELELGCSDGIYEKIDWSKLGGKEGFIPAGVSDCGVGAIVWSTGMAFDSD
KIKDGPKTWADFWDTTKFPGKRGLRKGAKYTLEFALMADGVPAADVYKVLKTKEGVERAFKKLDALKADIVWWEAGAQPL
QLLSSGQVAMTSVYNGRITAINRTEGKHFGFVFPGSIYAVDSWVVLKGSPNKDAAMAFIAFASQADNQAKLPEYIAYGLP
NIAAAKLVPEKYAAELPTTPDNLKQAIALDVGFWTDNSEELSKRFNAWLAR
>Mature_371_residues
MLSLRTTPTNAMMSNRTDRAASRAPIRVATAILVAAAALISVAASAQARDLTVVSWGGNYQDAQRKIYFQPFAAKLGATV
LDESWDGGYGVLQAKVKAGVPNWDAVQVEAEELELGCSDGIYEKIDWSKLGGKEGFIPAGVSDCGVGAIVWSTGMAFDSD
KIKDGPKTWADFWDTTKFPGKRGLRKGAKYTLEFALMADGVPAADVYKVLKTKEGVERAFKKLDALKADIVWWEAGAQPL
QLLSSGQVAMTSVYNGRITAINRTEGKHFGFVFPGSIYAVDSWVVLKGSPNKDAAMAFIAFASQADNQAKLPEYIAYGLP
NIAAAKLVPEKYAAELPTTPDNLKQAIALDVGFWTDNSEELSKRFNAWLAR

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40091; Mature: 40091

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLRTTPTNAMMSNRTDRAASRAPIRVATAILVAAAALISVAASAQARDLTVVSWGGNY
CCCCCCCCCHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCH
QDAQRKIYFQPFAAKLGATVLDESWDGGYGVLQAKVKAGVPNWDAVQVEAEELELGCSDG
HCCCCEEEECHHHHHHCCHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEHHHHCCCCCCC
IYEKIDWSKLGGKEGFIPAGVSDCGVGAIVWSTGMAFDSDKIKDGPKTWADFWDTTKFPG
CHHHCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCC
KRGLRKGAKYTLEFALMADGVPAADVYKVLKTKEGVERAFKKLDALKADIVWWEAGAQPL
HHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHH
QLLSSGQVAMTSVYNGRITAINRTEGKHFGFVFPGSIYAVDSWVVLKGSPNKDAAMAFIA
HHHCCCCEEEEEEECCEEEEEECCCCCEEEEEECCCEEEEEEEEEEECCCCCHHHHHHHH
FASQADNQAKLPEYIAYGLPNIAAAKLVPEKYAAELPTTPDNLKQAIALDVGFWTDNSEE
HHHCCCCCCCCHHHHHHCCCCHHHHHHCCHHHHHCCCCCHHHHHHHHEEEEEECCCCHHH
LSKRFNAWLAR
HHHHHHHHHCC
>Mature Secondary Structure
MLSLRTTPTNAMMSNRTDRAASRAPIRVATAILVAAAALISVAASAQARDLTVVSWGGNY
CCCCCCCCCHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCH
QDAQRKIYFQPFAAKLGATVLDESWDGGYGVLQAKVKAGVPNWDAVQVEAEELELGCSDG
HCCCCEEEECHHHHHHCCHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEHHHHCCCCCCC
IYEKIDWSKLGGKEGFIPAGVSDCGVGAIVWSTGMAFDSDKIKDGPKTWADFWDTTKFPG
CHHHCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCC
KRGLRKGAKYTLEFALMADGVPAADVYKVLKTKEGVERAFKKLDALKADIVWWEAGAQPL
HHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHH
QLLSSGQVAMTSVYNGRITAINRTEGKHFGFVFPGSIYAVDSWVVLKGSPNKDAAMAFIA
HHHCCCCEEEEEEECCEEEEEECCCCCEEEEEECCCEEEEEEEEEEECCCCCHHHHHHHH
FASQADNQAKLPEYIAYGLPNIAAAKLVPEKYAAELPTTPDNLKQAIALDVGFWTDNSEE
HHHCCCCCCCCHHHHHHCCCCHHHHHHCCHHHHHCCCCCHHHHHHHHEEEEEECCCCHHH
LSKRFNAWLAR
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA