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The map label for this gene is acrA [H]

Identifier: 146342989

GI number: 146342989

Start: 6400786

End: 6402018

Strand: Direct

Name: acrA [H]

Synonym: BRADO6172

Alternate gene names: 146342989

Gene position: 6400786-6402018 (Clockwise)

Preceding gene: 146342987

Following gene: 146342990

Centisome position: 85.84

GC content: 69.91

Gene sequence:

>1233_bases
ATGCCTTCGCCCACCCCCGTTTCCTCCGGCCGCCTGAAGCGGTGGCTCAGCACCGCTGCCGTCGTCGGCGCGGTGATCGC
CGCCGGCTCTTATGCCGGCTCGCACTATTTCCATGTCAGCCCGATGCCGCAGGCCGCCCGGGCCGCCGCGCCTGAGCAGG
CCGTCGCGGTGACCGTCTCCGTTGTCGAGCCGCGCAAGGCCAGCCTGTGGGATGAATTCTCCGGCCGCCTCGAAGCGGTC
GAGCGCGTCGAGGTCCGGCCGCGCGTTGCCGGCGCCATCCTCGCCACCAATTTTACCGAGGGCTCGCTGGTGAAGGCCGG
CGACGTCCTGTTCAAGATCGATCCGGCGCCCTATGCCGCCGAGGTCGACCGTGCGCAGGCACAACTCGAAGCCGCCCGCG
CCCGCGCGGCCTTCGCCGCCAGCGAGCTGGAGCGCGGCGCCCAGCTGGTCGGCAATTCGATCGTGACCAAGCGCGACTAC
GACCAGCGCGACAACGGCAATCGCGAGGCGATCGCCAACGTCAAGGCGGCGGAAGCCGCGCTGCAGGCAGCGAAGCTCAA
TCTCGACTACACGCAGGTTCGCGCGCCGGTCGACGGCCGGGTCGGCCGGATCGAGGTCACCGTCGGCAACCTCGTCGCCG
CCGGCTCCGCCTCGCCGGTGCTGACCAACCTCGTTTCCGTCAATCCGATCTATGCGAGCTTCGACGCCACCGAGGATGTC
GTGCTGCGGGCGCTGGCGGCTGTGGCCGATGGGTCTGGCAAGCGCGGCAATCTCGACCGCATCCCGGTGGAGATGACGAC
GTCGGGCGGCATCACGGCCTCCGGCCACATCCAGCTGATCGACAACCAGGTGCAGGGCCAGACCGGCACCATCCGCGTCC
GCGCCGTGTTCGAGAACGAGACCGGCACCTTGATCCCCGGCCAGTTCGCCCGCGTCCGCATGGGCCAGCCGCAACAGCAA
TCCCTGGTGCTGCTCGACGAGCGCGCCATCGGCACCGACCAGGACAAGAAGTTCGTGATGGTGGTCGGCGACGACGAGCG
CGCGGTGTACCGCCCGGTCACGATCGGCGGCAGCATCGAGGGTCTGCGCATCATCTCGGCCGGGCTGAAGCCTGGTGAGC
GCGTGGTCGTCAACGGCCTGCAACGGGTGCGCCCGGGCGCGCTGCTGAAGACGCAGGTCGCCGAGATGGGCGCGCGGGAT
GGGGCGGATGCGAAACAGGTGGCGCAGCGCTGA

Upstream 100 bases:

>100_bases
GTTCCGTCTAGGTTTTTCGTAGCATCCGTTACATAAGTCTCTTGCGAACGAGTTGCCAGCGCCACATTTGTAGCGAGCAC
TACAAATCTGGAGCGCGCCA

Downstream 100 bases:

>100_bases
GCGTCAATGGATCACGGCTGCCAACGCCACCCACAGCCGTCGTCCCGGCGAACGCCGGGACCCATAACCACCGGACTTGC
TTATCGACAAAGAAAGATGA

Product: AcrB/AcrD/AcrF family mulitdrug efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MPSPTPVSSGRLKRWLSTAAVVGAVIAAGSYAGSHYFHVSPMPQAARAAAPEQAVAVTVSVVEPRKASLWDEFSGRLEAV
ERVEVRPRVAGAILATNFTEGSLVKAGDVLFKIDPAPYAAEVDRAQAQLEAARARAAFAASELERGAQLVGNSIVTKRDY
DQRDNGNREAIANVKAAEAALQAAKLNLDYTQVRAPVDGRVGRIEVTVGNLVAAGSASPVLTNLVSVNPIYASFDATEDV
VLRALAAVADGSGKRGNLDRIPVEMTTSGGITASGHIQLIDNQVQGQTGTIRVRAVFENETGTLIPGQFARVRMGQPQQQ
SLVLLDERAIGTDQDKKFVMVVGDDERAVYRPVTIGGSIEGLRIISAGLKPGERVVVNGLQRVRPGALLKTQVAEMGARD
GADAKQVAQR

Sequences:

>Translated_410_residues
MPSPTPVSSGRLKRWLSTAAVVGAVIAAGSYAGSHYFHVSPMPQAARAAAPEQAVAVTVSVVEPRKASLWDEFSGRLEAV
ERVEVRPRVAGAILATNFTEGSLVKAGDVLFKIDPAPYAAEVDRAQAQLEAARARAAFAASELERGAQLVGNSIVTKRDY
DQRDNGNREAIANVKAAEAALQAAKLNLDYTQVRAPVDGRVGRIEVTVGNLVAAGSASPVLTNLVSVNPIYASFDATEDV
VLRALAAVADGSGKRGNLDRIPVEMTTSGGITASGHIQLIDNQVQGQTGTIRVRAVFENETGTLIPGQFARVRMGQPQQQ
SLVLLDERAIGTDQDKKFVMVVGDDERAVYRPVTIGGSIEGLRIISAGLKPGERVVVNGLQRVRPGALLKTQVAEMGARD
GADAKQVAQR
>Mature_409_residues
PSPTPVSSGRLKRWLSTAAVVGAVIAAGSYAGSHYFHVSPMPQAARAAAPEQAVAVTVSVVEPRKASLWDEFSGRLEAVE
RVEVRPRVAGAILATNFTEGSLVKAGDVLFKIDPAPYAAEVDRAQAQLEAARARAAFAASELERGAQLVGNSIVTKRDYD
QRDNGNREAIANVKAAEAALQAAKLNLDYTQVRAPVDGRVGRIEVTVGNLVAAGSASPVLTNLVSVNPIYASFDATEDVV
LRALAAVADGSGKRGNLDRIPVEMTTSGGITASGHIQLIDNQVQGQTGTIRVRAVFENETGTLIPGQFARVRMGQPQQQS
LVLLDERAIGTDQDKKFVMVVGDDERAVYRPVTIGGSIEGLRIISAGLKPGERVVVNGLQRVRPGALLKTQVAEMGARDG
ADAKQVAQR

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=344, Percent_Identity=36.9186046511628, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1789665, Length=337, Percent_Identity=37.0919881305638, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789929, Length=352, Percent_Identity=32.6704545454545, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=32.7102803738318, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI87081951, Length=154, Percent_Identity=32.4675324675325, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790519, Length=212, Percent_Identity=28.3018867924528, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789637, Length=149, Percent_Identity=31.5436241610738, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43286; Mature: 43154

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPTPVSSGRLKRWLSTAAVVGAVIAAGSYAGSHYFHVSPMPQAARAAAPEQAVAVTVS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCEEEEEEE
VVEPRKASLWDEFSGRLEAVERVEVRPRVAGAILATNFTEGSLVKAGDVLFKIDPAPYAA
EECCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCEEEECCEEEEECCCCCHH
EVDRAQAQLEAARARAAFAASELERGAQLVGNSIVTKRDYDQRDNGNREAIANVKAAEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHH
LQAAKLNLDYTQVRAPVDGRVGRIEVTVGNLVAAGSASPVLTNLVSVNPIYASFDATEDV
HHHHHCCCCHHHEECCCCCCCEEEEEEECCEEECCCCCHHHHHHEECCCEEEECCCHHHH
VLRALAAVADGSGKRGNLDRIPVEMTTSGGITASGHIQLIDNQVQGQTGTIRVRAVFENE
HHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEECCEEEEEECCCCCCCCEEEEEEEEECC
TGTLIPGQFARVRMGQPQQQSLVLLDERAIGTDQDKKFVMVVGDDERAVYRPVTIGGSIE
CCCCCCCCHHEEECCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCC
GLRIISAGLKPGERVVVNGLQRVRPGALLKTQVAEMGARDGADAKQVAQR
CEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHCC
>Mature Secondary Structure 
PSPTPVSSGRLKRWLSTAAVVGAVIAAGSYAGSHYFHVSPMPQAARAAAPEQAVAVTVS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCEEEEEEE
VVEPRKASLWDEFSGRLEAVERVEVRPRVAGAILATNFTEGSLVKAGDVLFKIDPAPYAA
EECCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCEEEECCEEEEECCCCCHH
EVDRAQAQLEAARARAAFAASELERGAQLVGNSIVTKRDYDQRDNGNREAIANVKAAEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHH
LQAAKLNLDYTQVRAPVDGRVGRIEVTVGNLVAAGSASPVLTNLVSVNPIYASFDATEDV
HHHHHCCCCHHHEECCCCCCCEEEEEEECCEEECCCCCHHHHHHEECCCEEEECCCHHHH
VLRALAAVADGSGKRGNLDRIPVEMTTSGGITASGHIQLIDNQVQGQTGTIRVRAVFENE
HHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEECCEEEEEECCCCCCCCEEEEEEEEECC
TGTLIPGQFARVRMGQPQQQSLVLLDERAIGTDQDKKFVMVVGDDERAVYRPVTIGGSIE
CCCCCCCCHHEEECCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCC
GLRIISAGLKPGERVVVNGLQRVRPGALLKTQVAEMGARDGADAKQVAQR
CEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]