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The map label for this gene is glcD [H]

Identifier: 146342948

GI number: 146342948

Start: 6358221

End: 6359714

Strand: Reverse

Name: glcD [H]

Synonym: BRADO6131

Alternate gene names: 146342948

Gene position: 6359714-6358221 (Counterclockwise)

Preceding gene: 146342950

Following gene: 146342942

Centisome position: 85.29

GC content: 66.6

Gene sequence:

>1494_bases
ATGGCCATCATGATGCCGGAAGCTGATCAGGCGGTGCTGGCGCGCCGCGGCGACATCGTCGCTGCACTGCGCGCGATCGT
GCCGGGCGAGGGCGTCATCGACAGTGCCGCCGAGATGCTGCCCTATGAGTCCGACGGATTGATGGCCTATCGCCAGCCGC
CGATGGTCGTGGTGCTGCCGGAGACCACGGCACAGGTTTCGCAGGTTCTGAGATATTGCCACGGCCAAGGCATCAAGGTG
GTTCCGCGCGGCTCCGGAACCTCGCTGTCCGGCGGTGCCTTGCCGCTGGCTGATGGCGTGCTGCTTGGGCTGGGCAAGTT
CAAGCGCATCCGCGAGATCGACTTCGATAACCGCGTCGTCGTCACCGAGCCTGGAGTCACCAACCTCGCCATCAGCCAGG
CGGTGGCGCATGCCGGCTTCTACTACGCGCCCGACCCGTCGTCGCAGATCGCCTGCTCGATCGGCGGCAATGTCGCGGAG
AATTCCGGCGGCGTGCACTGCCTGAAATACGGCATGACCACCAACAACGTGCTCGGCTGCGAGCTCGTGCTGATGAATGG
CGAGGTGTTGCGCATCGGAGGCAAGTGCACGGAGAATGCCGGCTATGATCTGATGGGCCTGATCACCGGCTCGGAGGGGC
TGCTCGGCGTGGTCACCGAGGTCACCGTGCGCATCCTGCAAAAACCGGAGACGGCGCGCGCGCTGATGGTCGGCTTCACG
CAAGTCGAGGCCGCCGGCGAATGCGTCGCGCGCATCATCGGCGCCGGCATCATCCCCGGCGGCATGGAGATGATGGACAA
GCCGGCGATCCACGCCGCCGAGGCCTTCGTCCATGCCGGCTATCCGCTCGACGTCGAGGCGCTCTTGATCATCGAGCTCG
ACGGCCCCGGCGTCGAGGTCGACGAGCTGATCAGTCGCGTCGAGGCCATCGCCAACGGCTGCGGCTCGACCACCTGCCGG
ATCTCCACCTCCGAGGCCGAGCGCAACCTGTTCTGGGCCGGCCGCAAGGCGGCGTTCCCCGCGGTCGGGCGCATCTCGCC
GGACTATCTCTGCATGGACGGCACCATCCCGCGCGGCGCGCTGCCGAAGGCGCTCGCGCGCATCCGCGAGCTGTCGGAAA
AATATCAGCTCGGCTGCGCCAACGTCTTCCATGCCGGCGACGGCAATCTGCATCCGCTGATCCTCTACGACGCCAACAAG
CCCGGCGAGATGGAGCGCGCCGAAGCCTTCGGCGCCGACATCCTGCGCTGCTGCGTCGAGCTCGGCGGCGTCCTCACCGG
CGAGCACGGTGTCGGCATCGAGAAGCGCGACCTGATGCCCGACATGTTCACCGAGATCGACCTCAACCAGCAGCAGCGCG
TGAAATGCGCGTTCGATCATTTGGGCCTGCTGAATCCGGGCAAGGTGTTTCCGACCCTGCACCGCTGCGCCGAGCTCGGA
CGGATGCACGTGCACGCCGGCAAGCTGGCGTTTCCGGATCTGCCGCGGTTCTGA

Upstream 100 bases:

>100_bases
CGAAGCTTGCTCTGTCGCAAACAACGCAGCTGATCCGCTGCCTGTTCAAACCGTTTCCGTTGTGCTTGATATCCAACAGA
AATCGATCCGGAGGACCACG

Downstream 100 bases:

>100_bases
GGCGCGCAGGCCTTTCCGCGCCGTCATTGCGAGCGCAGCGAAGCAATCCAGGGGCGTAGGCGGGACTCTGGATTGCTTCC
GCCTTCGAGCTTCGGCGGAC

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MAIMMPEADQAVLARRGDIVAALRAIVPGEGVIDSAAEMLPYESDGLMAYRQPPMVVVLPETTAQVSQVLRYCHGQGIKV
VPRGSGTSLSGGALPLADGVLLGLGKFKRIREIDFDNRVVVTEPGVTNLAISQAVAHAGFYYAPDPSSQIACSIGGNVAE
NSGGVHCLKYGMTTNNVLGCELVLMNGEVLRIGGKCTENAGYDLMGLITGSEGLLGVVTEVTVRILQKPETARALMVGFT
QVEAAGECVARIIGAGIIPGGMEMMDKPAIHAAEAFVHAGYPLDVEALLIIELDGPGVEVDELISRVEAIANGCGSTTCR
ISTSEAERNLFWAGRKAAFPAVGRISPDYLCMDGTIPRGALPKALARIRELSEKYQLGCANVFHAGDGNLHPLILYDANK
PGEMERAEAFGADILRCCVELGGVLTGEHGVGIEKRDLMPDMFTEIDLNQQQRVKCAFDHLGLLNPGKVFPTLHRCAELG
RMHVHAGKLAFPDLPRF

Sequences:

>Translated_497_residues
MAIMMPEADQAVLARRGDIVAALRAIVPGEGVIDSAAEMLPYESDGLMAYRQPPMVVVLPETTAQVSQVLRYCHGQGIKV
VPRGSGTSLSGGALPLADGVLLGLGKFKRIREIDFDNRVVVTEPGVTNLAISQAVAHAGFYYAPDPSSQIACSIGGNVAE
NSGGVHCLKYGMTTNNVLGCELVLMNGEVLRIGGKCTENAGYDLMGLITGSEGLLGVVTEVTVRILQKPETARALMVGFT
QVEAAGECVARIIGAGIIPGGMEMMDKPAIHAAEAFVHAGYPLDVEALLIIELDGPGVEVDELISRVEAIANGCGSTTCR
ISTSEAERNLFWAGRKAAFPAVGRISPDYLCMDGTIPRGALPKALARIRELSEKYQLGCANVFHAGDGNLHPLILYDANK
PGEMERAEAFGADILRCCVELGGVLTGEHGVGIEKRDLMPDMFTEIDLNQQQRVKCAFDHLGLLNPGKVFPTLHRCAELG
RMHVHAGKLAFPDLPRF
>Mature_496_residues
AIMMPEADQAVLARRGDIVAALRAIVPGEGVIDSAAEMLPYESDGLMAYRQPPMVVVLPETTAQVSQVLRYCHGQGIKVV
PRGSGTSLSGGALPLADGVLLGLGKFKRIREIDFDNRVVVTEPGVTNLAISQAVAHAGFYYAPDPSSQIACSIGGNVAEN
SGGVHCLKYGMTTNNVLGCELVLMNGEVLRIGGKCTENAGYDLMGLITGSEGLLGVVTEVTVRILQKPETARALMVGFTQ
VEAAGECVARIIGAGIIPGGMEMMDKPAIHAAEAFVHAGYPLDVEALLIIELDGPGVEVDELISRVEAIANGCGSTTCRI
STSEAERNLFWAGRKAAFPAVGRISPDYLCMDGTIPRGALPKALARIRELSEKYQLGCANVFHAGDGNLHPLILYDANKP
GEMERAEAFGADILRCCVELGGVLTGEHGVGIEKRDLMPDMFTEIDLNQQQRVKCAFDHLGLLNPGKVFPTLHRCAELGR
MHVHAGKLAFPDLPRF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=459, Percent_Identity=30.9368191721133, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI37595754, Length=482, Percent_Identity=29.6680497925311, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI119964728, Length=460, Percent_Identity=28.9130434782609, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI4501993, Length=250, Percent_Identity=26, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1789351, Length=482, Percent_Identity=57.8838174273859, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI1787977, Length=519, Percent_Identity=25.0481695568401, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI48994907, Length=486, Percent_Identity=24.4855967078189, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71992373, Length=431, Percent_Identity=29.0023201856148, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI71992381, Length=428, Percent_Identity=28.9719626168224, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI71992392, Length=371, Percent_Identity=28.0323450134771, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17534361, Length=449, Percent_Identity=26.2806236080178, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17556096, Length=261, Percent_Identity=23.7547892720307, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320027, Length=437, Percent_Identity=28.3752860411899, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320764, Length=472, Percent_Identity=25.635593220339, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6320023, Length=474, Percent_Identity=26.3713080168776, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18921117, Length=439, Percent_Identity=27.1070615034169, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24639277, Length=439, Percent_Identity=27.1070615034169, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24639275, Length=439, Percent_Identity=27.1070615034169, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24653753, Length=231, Percent_Identity=25.1082251082251, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 52855; Mature: 52724

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIMMPEADQAVLARRGDIVAALRAIVPGEGVIDSAAEMLPYESDGLMAYRQPPMVVVLP
CEEECCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEECCCCEEEEEC
ETTAQVSQVLRYCHGQGIKVVPRGSGTSLSGGALPLADGVLLGLGKFKRIREIDFDNRVV
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHCHHHHCCHHHHHHHHCCCCCEEE
VTEPGVTNLAISQAVAHAGFYYAPDPSSQIACSIGGNVAENSGGVHCLKYGMTTNNVLGC
EECCCCCHHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEE
ELVLMNGEVLRIGGKCTENAGYDLMGLITGSEGLLGVVTEVTVRILQKPETARALMVGFT
EEEEECCCEEEECCEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
QVEAAGECVARIIGAGIIPGGMEMMDKPAIHAAEAFVHAGYPLDVEALLIIELDGPGVEV
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCCH
DELISRVEAIANGCGSTTCRISTSEAERNLFWAGRKAAFPAVGRISPDYLCMDGTIPRGA
HHHHHHHHHHHCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCEEEECCCCCCCC
LPKALARIRELSEKYQLGCANVFHAGDGNLHPLILYDANKPGEMERAEAFGADILRCCVE
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LGGVLTGEHGVGIEKRDLMPDMFTEIDLNQQQRVKCAFDHLGLLNPGKVFPTLHRCAELG
HCCEEECCCCCCCCHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
RMHVHAGKLAFPDLPRF
CEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AIMMPEADQAVLARRGDIVAALRAIVPGEGVIDSAAEMLPYESDGLMAYRQPPMVVVLP
EEECCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEECCCCEEEEEC
ETTAQVSQVLRYCHGQGIKVVPRGSGTSLSGGALPLADGVLLGLGKFKRIREIDFDNRVV
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHCHHHHCCHHHHHHHHCCCCCEEE
VTEPGVTNLAISQAVAHAGFYYAPDPSSQIACSIGGNVAENSGGVHCLKYGMTTNNVLGC
EECCCCCHHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEE
ELVLMNGEVLRIGGKCTENAGYDLMGLITGSEGLLGVVTEVTVRILQKPETARALMVGFT
EEEEECCCEEEECCEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
QVEAAGECVARIIGAGIIPGGMEMMDKPAIHAAEAFVHAGYPLDVEALLIIELDGPGVEV
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCCH
DELISRVEAIANGCGSTTCRISTSEAERNLFWAGRKAAFPAVGRISPDYLCMDGTIPRGA
HHHHHHHHHHHCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCEEEECCCCCCCC
LPKALARIRELSEKYQLGCANVFHAGDGNLHPLILYDANKPGEMERAEAFGADILRCCVE
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LGGVLTGEHGVGIEKRDLMPDMFTEIDLNQQQRVKCAFDHLGLLNPGKVFPTLHRCAELG
HCCEEECCCCCCCCHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
RMHVHAGKLAFPDLPRF
CEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]