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The map label for this gene is glcF [H]

Identifier: 146342941

GI number: 146342941

Start: 6351210

End: 6352529

Strand: Reverse

Name: glcF [H]

Synonym: BRADO6122

Alternate gene names: 146342941

Gene position: 6352529-6351210 (Counterclockwise)

Preceding gene: 146342942

Following gene: 146342940

Centisome position: 85.19

GC content: 67.05

Gene sequence:

>1320_bases
ATGAAGACCGAGTTCACCCTGGCGCAGCTCGCCGATCCCGACATCGCGGTCGCCGACAAGATCCTGCGCGCCTGCGTGCA
TTGCGGCTTCTGCACCGCGACCTGTCCGACCTATGTGCTGCTCGGCGACGAGCTCGACAGCCCGCGCGGCCGCATCTACC
TGATCAAGCAGATGCTGGAGAAGGACCAGGCGCCGACATCGGATGTCGTCAAGCACATCGACCGCTGCCTGTCGTGCCTG
TCCTGCATGACGACCTGTCCGTCGGGCGTGCACTACATGCACCTCGTCGACCAGGCGCGGGTGCGGATCGAGGAGACCTA
TCAGCGGCCGCTGACCGACCGCCTGCTCCGCGCCGTGCTGGCCTATGTACTGCCGCGGCCGCAGCTGTTTCGGCTGGCGA
TGACCCTGGCCCGGCTCGGCCGGCCGTTCAAGGCGCTGCTGCCGGCGTCGTCGGCGTCAGCGCCGACGCCGAGCCTGTGG
CGGCGGCTGCGGGCGATGCTGGAGCTCGCGCCGGCCGCGGCGCTGCCGCCGCCGGGGCCCTCGGGCGGCAGCGTGTTTCC
CGCGCAGGGCGCGCGGCGCGGCCGTGTCGCGTTGCTGCAGGGCTGCGCGCAGGCCGCGCTGGCGCCGCGAATCAACCAGG
CTGCGATCAACGTGCTGACGCGGCACGGCATCGAGGTGGTGCTGGTCAAGGACGAGCAATGCTGCGGTGCGCTCACCCAT
CACATGGGGCAGGACGACGATGCGATGGCGCGGGCGCGGGCCAATGTCGGCGTATGGACCGCCGAGGCATCGCGCGGCGG
GCTCGATGCGATCCTGGTGACGACATCGGGCTGCGGCACCGTCATCAAGGACTACGGCCACATGCTGCGCGAGGACCCGC
AATTTGCAGCACCTGCGGCAGCCGTCTCGGCGATGGCGAAGGACATCAGCGAGTATCTCGCAGGGCTCGAATTGAAGACG
ACGGCGGAGTCGCGCGGCCTCAGCGTCGCCTATCACTCCGCATGCTCGCTGCAACACGGGCAGAAAATTACGAGCATTCC
GAAAGAATTGCTTTCCAAGTCCGGCTTCGTGGTGAAAGATATACCGGAGAGTCATTTGTGTTGCGGTTCGGCGGGGACCT
ACAACATTCTCCAGCCTGACATCGCGATGAGACTGCGCGATCGCAAGGTCGCCAACATCGCGACGGTCAAGCCGGATGTC
ATCGCGGCAGGCAATATCGGCTGCATGATGCAGATTGCCAGCGGCACCGGCATCCCCGTCGTCCACACCGTTGAGCTTCT
CGACTGGGCGACGGGCGGTCCGCGCCCGGGACTGAATTGA

Upstream 100 bases:

>100_bases
CGCAGCCGCCCGGCGTCGCCGCGCTGGAGGAGCGGGTGCGCGTGTCGTTCGATCCCAAGGCCATTCTCAACCGGGGCCGT
CTGCGTCGGAGCGTTGCGTC

Downstream 100 bases:

>100_bases
GAGATGGGCGCGAGCGGTCTGCCGGCCCGTCCCGCCGATGACGCGTTTCACTCGGCGCAACTGGAGGATTTCGGATGGCG
AAGGACAAGAAAGACAAGAA

Product: glycolate oxidase subunit, (Fe-S)protein, GlcF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKTEFTLAQLADPDIAVADKILRACVHCGFCTATCPTYVLLGDELDSPRGRIYLIKQMLEKDQAPTSDVVKHIDRCLSCL
SCMTTCPSGVHYMHLVDQARVRIEETYQRPLTDRLLRAVLAYVLPRPQLFRLAMTLARLGRPFKALLPASSASAPTPSLW
RRLRAMLELAPAAALPPPGPSGGSVFPAQGARRGRVALLQGCAQAALAPRINQAAINVLTRHGIEVVLVKDEQCCGALTH
HMGQDDDAMARARANVGVWTAEASRGGLDAILVTTSGCGTVIKDYGHMLREDPQFAAPAAAVSAMAKDISEYLAGLELKT
TAESRGLSVAYHSACSLQHGQKITSIPKELLSKSGFVVKDIPESHLCCGSAGTYNILQPDIAMRLRDRKVANIATVKPDV
IAAGNIGCMMQIASGTGIPVVHTVELLDWATGGPRPGLN

Sequences:

>Translated_439_residues
MKTEFTLAQLADPDIAVADKILRACVHCGFCTATCPTYVLLGDELDSPRGRIYLIKQMLEKDQAPTSDVVKHIDRCLSCL
SCMTTCPSGVHYMHLVDQARVRIEETYQRPLTDRLLRAVLAYVLPRPQLFRLAMTLARLGRPFKALLPASSASAPTPSLW
RRLRAMLELAPAAALPPPGPSGGSVFPAQGARRGRVALLQGCAQAALAPRINQAAINVLTRHGIEVVLVKDEQCCGALTH
HMGQDDDAMARARANVGVWTAEASRGGLDAILVTTSGCGTVIKDYGHMLREDPQFAAPAAAVSAMAKDISEYLAGLELKT
TAESRGLSVAYHSACSLQHGQKITSIPKELLSKSGFVVKDIPESHLCCGSAGTYNILQPDIAMRLRDRKVANIATVKPDV
IAAGNIGCMMQIASGTGIPVVHTVELLDWATGGPRPGLN
>Mature_439_residues
MKTEFTLAQLADPDIAVADKILRACVHCGFCTATCPTYVLLGDELDSPRGRIYLIKQMLEKDQAPTSDVVKHIDRCLSCL
SCMTTCPSGVHYMHLVDQARVRIEETYQRPLTDRLLRAVLAYVLPRPQLFRLAMTLARLGRPFKALLPASSASAPTPSLW
RRLRAMLELAPAAALPPPGPSGGSVFPAQGARRGRVALLQGCAQAALAPRINQAAINVLTRHGIEVVLVKDEQCCGALTH
HMGQDDDAMARARANVGVWTAEASRGGLDAILVTTSGCGTVIKDYGHMLREDPQFAAPAAAVSAMAKDISEYLAGLELKT
TAESRGLSVAYHSACSLQHGQKITSIPKELLSKSGFVVKDIPESHLCCGSAGTYNILQPDIAMRLRDRKVANIATVKPDV
IAAGNIGCMMQIASGTGIPVVHTVELLDWATGGPRPGLN

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=430, Percent_Identity=43.0232558139535, Blast_Score=341, Evalue=6e-95,
Organism=Escherichia coli, GI1786497, Length=243, Percent_Identity=23.0452674897119, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 46971; Mature: 46971

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEFTLAQLADPDIAVADKILRACVHCGFCTATCPTYVLLGDELDSPRGRIYLIKQMLE
CCCCCCHHHHCCCCHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCCHHHHHHHHHH
KDQAPTSDVVKHIDRCLSCLSCMTTCPSGVHYMHLVDQARVRIEETYQRPLTDRLLRAVL
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AYVLPRPQLFRLAMTLARLGRPFKALLPASSASAPTPSLWRRLRAMLELAPAAALPPPGP
HHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
SGGSVFPAQGARRGRVALLQGCAQAALAPRINQAAINVLTRHGIEVVLVKDEQCCGALTH
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHH
HMGQDDDAMARARANVGVWTAEASRGGLDAILVTTSGCGTVIKDYGHMLREDPQFAAPAA
HCCCCHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHH
AVSAMAKDISEYLAGLELKTTAESRGLSVAYHSACSLQHGQKITSIPKELLSKSGFVVKD
HHHHHHHHHHHHHHCCEEEECHHHCCCEEEHHHHHHHHCCCHHHHHHHHHHHCCCCEEEC
IPESHLCCGSAGTYNILQPDIAMRLRDRKVANIATVKPDVIAAGNIGCMMQIASGTGIPV
CCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCEEECCCCCEEEEECCCCCCCH
VHTVELLDWATGGPRPGLN
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKTEFTLAQLADPDIAVADKILRACVHCGFCTATCPTYVLLGDELDSPRGRIYLIKQMLE
CCCCCCHHHHCCCCHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCCHHHHHHHHHH
KDQAPTSDVVKHIDRCLSCLSCMTTCPSGVHYMHLVDQARVRIEETYQRPLTDRLLRAVL
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AYVLPRPQLFRLAMTLARLGRPFKALLPASSASAPTPSLWRRLRAMLELAPAAALPPPGP
HHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
SGGSVFPAQGARRGRVALLQGCAQAALAPRINQAAINVLTRHGIEVVLVKDEQCCGALTH
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHH
HMGQDDDAMARARANVGVWTAEASRGGLDAILVTTSGCGTVIKDYGHMLREDPQFAAPAA
HCCCCHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHH
AVSAMAKDISEYLAGLELKTTAESRGLSVAYHSACSLQHGQKITSIPKELLSKSGFVVKD
HHHHHHHHHHHHHHCCEEEECHHHCCCEEEHHHHHHHHCCCHHHHHHHHHHHCCCCEEEC
IPESHLCCGSAGTYNILQPDIAMRLRDRKVANIATVKPDVIAAGNIGCMMQIASGTGIPV
CCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCEEECCCCCEEEEECCCCCCCH
VHTVELLDWATGGPRPGLN
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]