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The map label for this gene is purD [H]

Identifier: 146342907

GI number: 146342907

Start: 6316971

End: 6318254

Strand: Reverse

Name: purD [H]

Synonym: BRADO6088

Alternate gene names: 146342907

Gene position: 6318254-6316971 (Counterclockwise)

Preceding gene: 146342908

Following gene: 146342906

Centisome position: 84.73

GC content: 65.03

Gene sequence:

>1284_bases
ATGAATATCCTCCTGATTGGTTCCGGCGGCCGCGAGCATGCTCTCGCCTGGAAGATCGCCGGCTCCCCGCTGCTCACCAA
GCTGTGGTGCGCTCCGGGCAATGCGGGGATTGCCAAGGAGGCGGAATGCGTCGCGCTCGATACGACCAACCATGCTGATG
TGATCGCCTTCTGCAGGGCGAACAAGGTCGATCTCGTGGTGGTAGGGCCGGAGACGCCGCTGGCGGCGGGAATCGTCGAC
GATGTCAGCGGCGCCGGCATCAAGATCTTTGGGCCGCGCAAGCTGGCCGCGCAGCTCGAGGGCTCGAAGGGCTTCACCAA
GGATCTCTGCGCCGAATACGGCATCCCGACCGGCGCTTACAGCCGCTTCGACAATGCGCCGGAGGCGCTGGCCTATGTCC
GCGAGCACGGCGCGCCGATCGTCGTGAAGGCCGACGGTTTGGCGGCGGGCAAGGGCGTGGTCGTCGCCAGGACGCTCGTC
GAAGCTGAAGAGGCTGTGCGGATGATGTTCGATGGCGGCTTCGGCGTTGCCGGCGCAGAGGTGGTCATCGAGGAGTTTCT
CGAAGGGCGCGAGGTCTCGTTCTTCGCGCTCTGTGACGGCGAGACTGCGATCCCGCTGGCCTCGGCGCAGGACCACAAGC
GGGTGTTCGACCACGACCAGGGGCCGAACACTGGCGGCATGGGCGCGTATTCGCCGACGCCGTTCGTCACGCAGGAAATT
CATGATCAGATCATGAAACGCATCATCCTGCCGACCATCGACGGCATGAATGCGCGCGGGACGCCCTTCAAGGGCATCCT
CTATGCCGGGCTGATGCTGACGGCCGAGGGACCGAAGCTGTTCGAATACAATGTCCGCTTCGGCGATCCGGAGTGCCAGG
TGCTGATGCTGCGGATGATGTCCGACCTGGTGCCGGCGATGCTCGCCGCATGCGATGGGCAATTGAAGAATTTCGACCTG
CGCTGGTTTCCCGACGCGGCGGTCACCGTGGTCATGGCCGCGGAAGGCTATCCCGGTGACTACGCCAAGGGGAGCGTGAT
CGAGGGGCTGGACGACGCTGCCAAGGTTGACGGCGTTGAAATCTTCCACGCTGGGACGGTGGTGAAGGATGGTTCGATCA
TCGCCAATGGCGGCCGCGTGCTGAACGTCTGCGCCACCGGCAAGACCATCACCGAGGCGCAGGCGCGCGCCTATGAGGCG
GTCGACCGCATCCGCTGGACCGATGGCTTCTGCCGCCGCGACATCGCCTGGCAGGCGCTGGCGCAGGAGCGTGGCCAAGG
TTGA

Upstream 100 bases:

>100_bases
GGAGCCGCCGCAAAGCCGCCCGAGCCATGATTTGACTCTGCGGCGAGGGCCGGGCAAGTGGGGCCTCCAGACCGACCCAC
TCCCTTTGACGGCATCGACG

Downstream 100 bases:

>100_bases
GATTCACCTCGGCGTCACAACCGGCGGGCCGTGCGAGCTCCACGTTGACAAGGACACGCATCCGCCTTGAGGCTATGTTT
CCCGCGAAAATTGTGGCAGT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNILLIGSGGREHALAWKIAGSPLLTKLWCAPGNAGIAKEAECVALDTTNHADVIAFCRANKVDLVVVGPETPLAAGIVD
DVSGAGIKIFGPRKLAAQLEGSKGFTKDLCAEYGIPTGAYSRFDNAPEALAYVREHGAPIVVKADGLAAGKGVVVARTLV
EAEEAVRMMFDGGFGVAGAEVVIEEFLEGREVSFFALCDGETAIPLASAQDHKRVFDHDQGPNTGGMGAYSPTPFVTQEI
HDQIMKRIILPTIDGMNARGTPFKGILYAGLMLTAEGPKLFEYNVRFGDPECQVLMLRMMSDLVPAMLAACDGQLKNFDL
RWFPDAAVTVVMAAEGYPGDYAKGSVIEGLDDAAKVDGVEIFHAGTVVKDGSIIANGGRVLNVCATGKTITEAQARAYEA
VDRIRWTDGFCRRDIAWQALAQERGQG

Sequences:

>Translated_427_residues
MNILLIGSGGREHALAWKIAGSPLLTKLWCAPGNAGIAKEAECVALDTTNHADVIAFCRANKVDLVVVGPETPLAAGIVD
DVSGAGIKIFGPRKLAAQLEGSKGFTKDLCAEYGIPTGAYSRFDNAPEALAYVREHGAPIVVKADGLAAGKGVVVARTLV
EAEEAVRMMFDGGFGVAGAEVVIEEFLEGREVSFFALCDGETAIPLASAQDHKRVFDHDQGPNTGGMGAYSPTPFVTQEI
HDQIMKRIILPTIDGMNARGTPFKGILYAGLMLTAEGPKLFEYNVRFGDPECQVLMLRMMSDLVPAMLAACDGQLKNFDL
RWFPDAAVTVVMAAEGYPGDYAKGSVIEGLDDAAKVDGVEIFHAGTVVKDGSIIANGGRVLNVCATGKTITEAQARAYEA
VDRIRWTDGFCRRDIAWQALAQERGQG
>Mature_427_residues
MNILLIGSGGREHALAWKIAGSPLLTKLWCAPGNAGIAKEAECVALDTTNHADVIAFCRANKVDLVVVGPETPLAAGIVD
DVSGAGIKIFGPRKLAAQLEGSKGFTKDLCAEYGIPTGAYSRFDNAPEALAYVREHGAPIVVKADGLAAGKGVVVARTLV
EAEEAVRMMFDGGFGVAGAEVVIEEFLEGREVSFFALCDGETAIPLASAQDHKRVFDHDQGPNTGGMGAYSPTPFVTQEI
HDQIMKRIILPTIDGMNARGTPFKGILYAGLMLTAEGPKLFEYNVRFGDPECQVLMLRMMSDLVPAMLAACDGQLKNFDL
RWFPDAAVTVVMAAEGYPGDYAKGSVIEGLDDAAKVDGVEIFHAGTVVKDGSIIANGGRVLNVCATGKTITEAQARAYEA
VDRIRWTDGFCRRDIAWQALAQERGQG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=46.1176470588235, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=46.2264150943396, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=46.2264150943396, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=46.2264150943396, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=55.4778554778555, Blast_Score=457, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=39.8126463700234, Blast_Score=283, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=42.0454545454545, Blast_Score=322, Evalue=5e-89,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=42.5581395348837, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=42.5581395348837, Blast_Score=293, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45380; Mature: 45380

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLIGSGGREHALAWKIAGSPLLTKLWCAPGNAGIAKEAECVALDTTNHADVIAFCRA
CEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEECC
NKVDLVVVGPETPLAAGIVDDVSGAGIKIFGPRKLAAQLEGSKGFTKDLCAEYGIPTGAY
CCEEEEEECCCCCCHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCHHHHHHHHCCCCCHH
SRFDNAPEALAYVREHGAPIVVKADGLAAGKGVVVARTLVEAEEAVRMMFDGGFGVAGAE
HHCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHH
VVIEEFLEGREVSFFALCDGETAIPLASAQDHKRVFDHDQGPNTGGMGAYSPTPFVTQEI
HHHHHHHCCCCEEEEEEECCCCCCEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
HDQIMKRIILPTIDGMNARGTPFKGILYAGLMLTAEGPKLFEYNVRFGDPECQVLMLRMM
HHHHHHHHHCCCCCCCCCCCCCCHHHEEEEEEEEECCCEEEEEEEEECCCHHHHHHHHHH
SDLVPAMLAACDGQLKNFDLRWFPDAAVTVVMAAEGYPGDYAKGSVIEGLDDAAKVDGVE
HHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCHHHCCCHHHHCCCEE
IFHAGTVVKDGSIIANGGRVLNVCATGKTITEAQARAYEAVDRIRWTDGFCRRDIAWQAL
EEECCEEEECCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AQERGQG
HHHCCCC
>Mature Secondary Structure
MNILLIGSGGREHALAWKIAGSPLLTKLWCAPGNAGIAKEAECVALDTTNHADVIAFCRA
CEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEECC
NKVDLVVVGPETPLAAGIVDDVSGAGIKIFGPRKLAAQLEGSKGFTKDLCAEYGIPTGAY
CCEEEEEECCCCCCHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCHHHHHHHHCCCCCHH
SRFDNAPEALAYVREHGAPIVVKADGLAAGKGVVVARTLVEAEEAVRMMFDGGFGVAGAE
HHCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHH
VVIEEFLEGREVSFFALCDGETAIPLASAQDHKRVFDHDQGPNTGGMGAYSPTPFVTQEI
HHHHHHHCCCCEEEEEEECCCCCCEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
HDQIMKRIILPTIDGMNARGTPFKGILYAGLMLTAEGPKLFEYNVRFGDPECQVLMLRMM
HHHHHHHHHCCCCCCCCCCCCCCHHHEEEEEEEEECCCEEEEEEEEECCCHHHHHHHHHH
SDLVPAMLAACDGQLKNFDLRWFPDAAVTVVMAAEGYPGDYAKGSVIEGLDDAAKVDGVE
HHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCHHHCCCHHHHCCCEE
IFHAGTVVKDGSIIANGGRVLNVCATGKTITEAQARAYEAVDRIRWTDGFCRRDIAWQAL
EEECCEEEECCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AQERGQG
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]