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The map label for this gene is rsmD [H]

Identifier: 146342899

GI number: 146342899

Start: 6306701

End: 6307255

Strand: Reverse

Name: rsmD [H]

Synonym: BRADO6080

Alternate gene names: 146342899

Gene position: 6307255-6306701 (Counterclockwise)

Preceding gene: 146342900

Following gene: 146342897

Centisome position: 84.59

GC content: 67.75

Gene sequence:

>555_bases
ATGCGGGTCGTCGGGGGACGGCTGAAGGGCCGCAACATCGCGTCGCCGGCGTCGCGCGATATCAGGCCGACGCAGGACCG
GCTGCGCGAGGCGCTGTTCAACATCCTCGTCCACGCCTACGACGATCCGATCGAGGGCGCGCGCGTGCTCGATCTGTTTG
CCGGCACCGGAGCGCTCGGCATCGAGGCGGTGTCGCGCGGCGCGGCGTTCACGCTGTTCGTAGACAATGGCGCCGAGGCG
CGGGCGCTGTTGCGCAACAATGTCGAGGCGCTGGGGCTCGGCGGTGTCACCAAGGTGTATCGCCGCGATGCCACCGATCT
TGGTCCGGCACATCCGGTCGAGCCGTTTGCGCTGGTGTTTCTCGATCCGCCCTATGGCAAGGGGCTGGCGGAGAAAGCAT
TGCTCTCGCTGCGCGACGGCGGCTGGCTCGTGCCATCGGCGCTGCTGGTCGTGGAGGAGTCCAAGGCCGCGGCGTTCAAG
GCGCCTGAGGGATTCGAGGAGCTTGAGCGTCGCGCTTACGACGACACCGAGTTCGTGTTCTTGCGCCGCTCGTGA

Upstream 100 bases:

>100_bases
GCGCTCGTGGCACCCCCACCCCCAACCCCTCCCCGCAAGGGGAAGGGGAGCGCAGCTCGCGCGCCGCTACGGCTTCTCCC
AAAATACTCAGAGGCGCGTG

Downstream 100 bases:

>100_bases
TCCCTGCTCGGTGTCGCCCCTGCGAACGCAGGGGCCCATAACCACAGGATTGCGTTGGGACGCACAAGCCTCGCCACGCC
ATTCTTTTTCCTCACATCTG

Product: putative methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MRVVGGRLKGRNIASPASRDIRPTQDRLREALFNILVHAYDDPIEGARVLDLFAGTGALGIEAVSRGAAFTLFVDNGAEA
RALLRNNVEALGLGGVTKVYRRDATDLGPAHPVEPFALVFLDPPYGKGLAEKALLSLRDGGWLVPSALLVVEESKAAAFK
APEGFEELERRAYDDTEFVFLRRS

Sequences:

>Translated_184_residues
MRVVGGRLKGRNIASPASRDIRPTQDRLREALFNILVHAYDDPIEGARVLDLFAGTGALGIEAVSRGAAFTLFVDNGAEA
RALLRNNVEALGLGGVTKVYRRDATDLGPAHPVEPFALVFLDPPYGKGLAEKALLSLRDGGWLVPSALLVVEESKAAAFK
APEGFEELERRAYDDTEFVFLRRS
>Mature_184_residues
MRVVGGRLKGRNIASPASRDIRPTQDRLREALFNILVHAYDDPIEGARVLDLFAGTGALGIEAVSRGAAFTLFVDNGAEA
RALLRNNVEALGLGGVTKVYRRDATDLGPAHPVEPFALVFLDPPYGKGLAEKALLSLRDGGWLVPSALLVVEESKAAAFK
APEGFEELERRAYDDTEFVFLRRS

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=152, Percent_Identity=39.4736842105263, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 19962; Mature: 19962

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVGGRLKGRNIASPASRDIRPTQDRLREALFNILVHAYDDPIEGARVLDLFAGTGALG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCCCHH
IEAVSRGAAFTLFVDNGAEARALLRNNVEALGLGGVTKVYRRDATDLGPAHPVEPFALVF
HHHHCCCCEEEEEEECCCHHHHHHHCCCCEECCCCHHHHHHCCCCCCCCCCCCCCEEEEE
LDPPYGKGLAEKALLSLRDGGWLVPSALLVVEESKAAAFKAPEGFEELERRAYDDTEFVF
ECCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEE
LRRS
EECC
>Mature Secondary Structure
MRVVGGRLKGRNIASPASRDIRPTQDRLREALFNILVHAYDDPIEGARVLDLFAGTGALG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCCCHH
IEAVSRGAAFTLFVDNGAEARALLRNNVEALGLGGVTKVYRRDATDLGPAHPVEPFALVF
HHHHCCCCEEEEEEECCCHHHHHHHCCCCEECCCCHHHHHHCCCCCCCCCCCCCCEEEEE
LDPPYGKGLAEKALLSLRDGGWLVPSALLVVEESKAAAFKAPEGFEELERRAYDDTEFVF
ECCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEE
LRRS
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]