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The map label for this gene is ribH2 [H]

Identifier: 146342853

GI number: 146342853

Start: 6257571

End: 6258176

Strand: Reverse

Name: ribH2 [H]

Synonym: BRADO6033

Alternate gene names: 146342853

Gene position: 6258176-6257571 (Counterclockwise)

Preceding gene: 146342856

Following gene: 146342852

Centisome position: 83.93

GC content: 65.02

Gene sequence:

>606_bases
ATGAGGATCGCCATGAATCAGATGTTGCAAGACTCCATCGACACCGCCACCACCACCGAAACTGCACAGCCGGCGGATAC
GCCGGTTGCGTCACCACCCGAGGAATTGCCTCCGGCGCCCTCGCATCCGCGCTTTGCCAAGCCGCAGCGCGTGGCCTTCG
TGCAGTCGGCCTGGCACCGCGACGTCGTCGCCGAATGCCGGGTGGCGTTTCTCGACGAGATCGAGGCGCGCCACATTCCA
CGCGACCGTGTCGACATCTTCGAGGTGCCGGGCTCGTTCGAGATCCCGCTGCATGCGCAACTGCTCGCCAAGACGCGTCG
CTACACCGCGATCGTCGCGGCGGGGCTCGTCGTCGACGGCGGCATCTATCGCCACGAATTTGTCGCCGACACCGTCATCA
AGGCGCTGATGGACGTACAGCTGCGCACCGAGGTGCCGGTATTCTCGGCGGTGCTGACCCCGCAACAATTCCACGAGACA
CAGGCGCATTTCGATTTCTTCCGGAAGCATTTCGCGACCAAGGGCGTCGAGGTGGCGACGGCCTGTGCGGAGACCCTGCT
GAGCCTCGAGCGCCTGCGCAGCCAGGTCGCGGCGGGGATCATCTGA

Upstream 100 bases:

>100_bases
GCCGACGGTCACAGTCCGGATGAAAGAGAACGGGTCCCGGCACCCCGCGCCATCCAGCGTGGGGGCGTCGTCGTTCCGTG
TGCCCTGATTCTGGTCTCTC

Downstream 100 bases:

>100_bases
GCGGGGGGATGGCGTGTGGCGGGCTATGGCCGGCCACATGCTTTCTCTCTGCACGATCGCACATCTTGCGACACGGGCGA
TCGGCTCCCCTCCCCGCAAG

Product: riboflavin synthase subunit beta

Products: NA

Alternate protein names: DMRL synthase 2; Lumazine synthase 2; Riboflavin synthase 2 beta chain [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MRIAMNQMLQDSIDTATTTETAQPADTPVASPPEELPPAPSHPRFAKPQRVAFVQSAWHRDVVAECRVAFLDEIEARHIP
RDRVDIFEVPGSFEIPLHAQLLAKTRRYTAIVAAGLVVDGGIYRHEFVADTVIKALMDVQLRTEVPVFSAVLTPQQFHET
QAHFDFFRKHFATKGVEVATACAETLLSLERLRSQVAAGII

Sequences:

>Translated_201_residues
MRIAMNQMLQDSIDTATTTETAQPADTPVASPPEELPPAPSHPRFAKPQRVAFVQSAWHRDVVAECRVAFLDEIEARHIP
RDRVDIFEVPGSFEIPLHAQLLAKTRRYTAIVAAGLVVDGGIYRHEFVADTVIKALMDVQLRTEVPVFSAVLTPQQFHET
QAHFDFFRKHFATKGVEVATACAETLLSLERLRSQVAAGII
>Mature_201_residues
MRIAMNQMLQDSIDTATTTETAQPADTPVASPPEELPPAPSHPRFAKPQRVAFVQSAWHRDVVAECRVAFLDEIEARHIP
RDRVDIFEVPGSFEIPLHAQLLAKTRRYTAIVAAGLVVDGGIYRHEFVADTVIKALMDVQLRTEVPVFSAVLTPQQFHET
QAHFDFFRKHFATKGVEVATACAETLLSLERLRSQVAAGII

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=117, Percent_Identity=29.9145299145299, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22352; Mature: 22352

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAMNQMLQDSIDTATTTETAQPADTPVASPPEELPPAPSHPRFAKPQRVAFVQSAWHR
CCCHHHHHHHHHHCHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHH
DVVAECRVAFLDEIEARHIPRDRVDIFEVPGSFEIPLHAQLLAKTRRYTAIVAAGLVVDG
HHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GIYRHEFVADTVIKALMDVQLRTEVPVFSAVLTPQQFHETQAHFDFFRKHFATKGVEVAT
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHH
ACAETLLSLERLRSQVAAGII
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIAMNQMLQDSIDTATTTETAQPADTPVASPPEELPPAPSHPRFAKPQRVAFVQSAWHR
CCCHHHHHHHHHHCHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHH
DVVAECRVAFLDEIEARHIPRDRVDIFEVPGSFEIPLHAQLLAKTRRYTAIVAAGLVVDG
HHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GIYRHEFVADTVIKALMDVQLRTEVPVFSAVLTPQQFHETQAHFDFFRKHFATKGVEVAT
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHH
ACAETLLSLERLRSQVAAGII
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]