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The map label for this gene is prs [H]

Identifier: 146342821

GI number: 146342821

Start: 6228200

End: 6229153

Strand: Reverse

Name: prs [H]

Synonym: BRADO6000

Alternate gene names: 146342821

Gene position: 6229153-6228200 (Counterclockwise)

Preceding gene: 146342822

Following gene: 146342820

Centisome position: 83.54

GC content: 66.46

Gene sequence:

>954_bases
ATGTCGGCCAAGAACGGCTCCATCAAGCTCGTCGCCGGCAACTCCAACCCGCCGCTCGCCGCCGCGATCGCCAGCGAATT
GGGATTGGCGCTGACGAAGGCCGTGGTTCGGCGCTTCGCGGACATGGAGATCTTCGTCGAGATCCAGGAGAACGTGCGCG
GCTCCGACGTCTTCATCATCCAGTCGACGTCCTATCCGGCGAACGACCTCCTGATGGAGCTCCTGATCATCACCGATGCG
TTGCGCCGCGCCTCCGCGCGCCGCATCACGGCCGTGGTTCCCTATTTCGGTTACGCCCGGCAGGACCGCAAGTCCGGCTC
GCGCACGCCGATCTCGGCCAAGCTGGTCGCCAACCTGATCACCCATGCCGGCGTCGACCGCGTCATGACGCTCGACCTGC
ATGCCGGCCAGATCCAGGGATTCTTCGACATCCCGACCGACAACCTGTTTGCCGCGCCGCTGATGGTGCGCGACATCAAG
GATCGTTTCGACCTCTCCAGCGTCATGGTGGTCTCGCCCGACGTCGGCGGCGTGGCGCGCGCCCGCGGCCTCGCCAAGCG
CATCAACGCGCCGCTGGCCATCGTCGACAAGCGCCGCGAGCGTCCCGGCGAATCCGAGGTCATGAATGTGATCGGCGACG
TCTCGGGCCGTACCTGCATCCTGATCGACGACATCGTCGATTCCGGCGGCACGCTGGTCAACGCCGCCGATGCGCTGCTG
AAATACGGCGCCAAGGACGTCTACGCCTACATCACCCATGGCGTGCTCTCCGGCGGCGCCGCGACCCGCATCAGCTCTTC
CAGGCTGAAGGAGCTCGTCATCACCGACTCCATCCTGCCGACCGAAGCCGTCCGCGGCGCCCCGAACGTCCGTGCCTTGC
CGATCGCTCCGCTGATCGCCGAAGCCATCGGCCGCACCGCGGCGGAGGAGTCGGTGTCGAGCCTGTTCGATTGA

Upstream 100 bases:

>100_bases
CAGTGTCCTTGATATCACCTCGCCCGTAGAAAACCGCCGGTTCCACGGATTGCCCGATATGCTCAACAAGGTCTCCAGCG
AACCGCGAGGAGAAATCGCG

Downstream 100 bases:

>100_bases
GGTTGATGGACTGAGGTTGTCGCGTGCGACGGGCTGGTCGAGGCCTTTCGGCTGACTATACAGAACAAGGCCGTCTGACC
CAGTCGGTCGTCCCCACATT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSAKNGSIKLVAGNSNPPLAAAIASELGLALTKAVVRRFADMEIFVEIQENVRGSDVFIIQSTSYPANDLLMELLIITDA
LRRASARRITAVVPYFGYARQDRKSGSRTPISAKLVANLITHAGVDRVMTLDLHAGQIQGFFDIPTDNLFAAPLMVRDIK
DRFDLSSVMVVSPDVGGVARARGLAKRINAPLAIVDKRRERPGESEVMNVIGDVSGRTCILIDDIVDSGGTLVNAADALL
KYGAKDVYAYITHGVLSGGAATRISSSRLKELVITDSILPTEAVRGAPNVRALPIAPLIAEAIGRTAAEESVSSLFD

Sequences:

>Translated_317_residues
MSAKNGSIKLVAGNSNPPLAAAIASELGLALTKAVVRRFADMEIFVEIQENVRGSDVFIIQSTSYPANDLLMELLIITDA
LRRASARRITAVVPYFGYARQDRKSGSRTPISAKLVANLITHAGVDRVMTLDLHAGQIQGFFDIPTDNLFAAPLMVRDIK
DRFDLSSVMVVSPDVGGVARARGLAKRINAPLAIVDKRRERPGESEVMNVIGDVSGRTCILIDDIVDSGGTLVNAADALL
KYGAKDVYAYITHGVLSGGAATRISSSRLKELVITDSILPTEAVRGAPNVRALPIAPLIAEAIGRTAAEESVSSLFD
>Mature_316_residues
SAKNGSIKLVAGNSNPPLAAAIASELGLALTKAVVRRFADMEIFVEIQENVRGSDVFIIQSTSYPANDLLMELLIITDAL
RRASARRITAVVPYFGYARQDRKSGSRTPISAKLVANLITHAGVDRVMTLDLHAGQIQGFFDIPTDNLFAAPLMVRDIKD
RFDLSSVMVVSPDVGGVARARGLAKRINAPLAIVDKRRERPGESEVMNVIGDVSGRTCILIDDIVDSGGTLVNAADALLK
YGAKDVYAYITHGVLSGGAATRISSSRLKELVITDSILPTEAVRGAPNVRALPIAPLIAEAIGRTAAEESVSSLFD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=311, Percent_Identity=46.6237942122186, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI28557709, Length=311, Percent_Identity=46.3022508038585, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI4506129, Length=311, Percent_Identity=46.3022508038585, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI84875539, Length=314, Percent_Identity=45.859872611465, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI4506133, Length=354, Percent_Identity=35.0282485875706, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI194018537, Length=353, Percent_Identity=34.5609065155807, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI310128524, Length=141, Percent_Identity=34.7517730496454, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310115209, Length=141, Percent_Identity=34.7517730496454, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310118259, Length=141, Percent_Identity=34.7517730496454, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310119946, Length=141, Percent_Identity=34.7517730496454, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=54.1666666666667, Blast_Score=342, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI25149168, Length=311, Percent_Identity=45.9807073954984, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17554702, Length=311, Percent_Identity=45.9807073954984, Blast_Score=292, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI71989924, Length=311, Percent_Identity=45.9807073954984, Blast_Score=290, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=45.7792207792208, Blast_Score=289, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17570245, Length=335, Percent_Identity=32.2388059701493, Blast_Score=181, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6320946, Length=313, Percent_Identity=47.2843450479233, Blast_Score=287, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6319403, Length=314, Percent_Identity=46.8152866242038, Blast_Score=282, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6321776, Length=312, Percent_Identity=46.1538461538462, Blast_Score=271, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6322667, Length=197, Percent_Identity=40.6091370558376, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324511, Length=86, Percent_Identity=43.0232558139535, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21355239, Length=311, Percent_Identity=46.9453376205788, Blast_Score=295, Evalue=2e-80,
Organism=Drosophila melanogaster, GI45551540, Length=334, Percent_Identity=43.7125748502994, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24651458, Length=354, Percent_Identity=32.7683615819209, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24651456, Length=354, Percent_Identity=32.7683615819209, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI281362873, Length=354, Percent_Identity=32.7683615819209, Blast_Score=188, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24651454, Length=354, Percent_Identity=32.7683615819209, Blast_Score=188, Evalue=5e-48,
Organism=Drosophila melanogaster, GI45552010, Length=373, Percent_Identity=32.171581769437, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24651462, Length=373, Percent_Identity=32.171581769437, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24651464, Length=373, Percent_Identity=32.171581769437, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 33780; Mature: 33649

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKNGSIKLVAGNSNPPLAAAIASELGLALTKAVVRRFADMEIFVEIQENVRGSDVFII
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEHHCCCCCEEEEE
QSTSYPANDLLMELLIITDALRRASARRITAVVPYFGYARQDRKSGSRTPISAKLVANLI
ECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHH
THAGVDRVMTLDLHAGQIQGFFDIPTDNLFAAPLMVRDIKDRFDLSSVMVVSPDVGGVAR
HHCCCCCEEEEEECCCCCCEEEECCCCCCEECHHHHHHHHHHCCCCEEEEECCCCCHHHH
ARGLAKRINAPLAIVDKRRERPGESEVMNVIGDVSGRTCILIDDIVDSGGTLVNAADALL
HHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCCCHHHHHHHH
KYGAKDVYAYITHGVLSGGAATRISSSRLKELVITDSILPTEAVRGAPNVRALPIAPLIA
HHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEECCHHHHHH
EAIGRTAAEESVSSLFD
HHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
SAKNGSIKLVAGNSNPPLAAAIASELGLALTKAVVRRFADMEIFVEIQENVRGSDVFII
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEHHCCCCCEEEEE
QSTSYPANDLLMELLIITDALRRASARRITAVVPYFGYARQDRKSGSRTPISAKLVANLI
ECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHH
THAGVDRVMTLDLHAGQIQGFFDIPTDNLFAAPLMVRDIKDRFDLSSVMVVSPDVGGVAR
HHCCCCCEEEEEECCCCCCEEEECCCCCCEECHHHHHHHHHHCCCCEEEEECCCCCHHHH
ARGLAKRINAPLAIVDKRRERPGESEVMNVIGDVSGRTCILIDDIVDSGGTLVNAADALL
HHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCCCHHHHHHHH
KYGAKDVYAYITHGVLSGGAATRISSSRLKELVITDSILPTEAVRGAPNVRALPIAPLIA
HHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEECCHHHHHH
EAIGRTAAEESVSSLFD
HHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]