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The map label for this gene is rutF [H]

Identifier: 146342737

GI number: 146342737

Start: 6128627

End: 6129202

Strand: Reverse

Name: rutF [H]

Synonym: BRADO5909

Alternate gene names: 146342737

Gene position: 6129202-6128627 (Counterclockwise)

Preceding gene: 146342738

Following gene: 146342736

Centisome position: 82.2

GC content: 67.88

Gene sequence:

>576_bases
ATGAACTCCATCGTCCGCCACATGCCTGATGCCACGGCGCTGCCGCCGGATTTCCGCTCTGCGATGCGCCAGCTGGTCGG
TGGCGTCAGCGTCATTACCGTCGGACGGGGCGACGAGATCACCGGCATGACCGTGAGCTCGCTGTCCTCGCTCGCGGTGG
ATCCGCCCTCGATCATCGTCAGCCTCAATCGCAATGCCTCGTCCTGGCCGATCCTGCAGCGGGAGCGCGTGTTCGGCGCC
AATCTGCTGGCCGCCGACCAGATCGGCGTGGCCGAGCGGTTCGCCGGTAAAGGCGGCCTGCGCGGCGCGGAACGTTTTGC
CGGCGCCGAATGGCGCAAACAGGTCACCGGCGTGCCGCTGCTGGTCGGTGCGCTGGCGACGCTCGACTGCGAGGTCGAGG
AGATCATCGAGCGGCATTCCCATGCCATCGTCATCGGCAAGGTGGTCCATGTCGCGGTGGCGCCGCAGTCGGCTGCCCTG
GCGTATTGGCATGGCAGCTATGTCACCGTCGATCGCGACGACGATCTCGGCCGGCTGGCGGCGCTGAGCGTGCCCGATCC
CCGGCTGACGACCTGA

Upstream 100 bases:

>100_bases
GCCCGCGACAGCGCGGCTCTGCCGAGCTGGCGGCGCTCGAAGGCTCGATCCTGGGCCAGTTGCTTGGACCGGGCCCGGCG
GGTTAGGAAAGATCAACGCA

Downstream 100 bases:

>100_bases
CCGGACGCGCTGGCGTCCGGGCGACGGACGCCGTAAAAGCCGCCGTCGGCGTCAGCGGCGGAATTCATGAAACGGATCGG
AACCTGGGCTCTCTACGTGG

Product: NAD(P)H-flavin oxidoreductase

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MNSIVRHMPDATALPPDFRSAMRQLVGGVSVITVGRGDEITGMTVSSLSSLAVDPPSIIVSLNRNASSWPILQRERVFGA
NLLAADQIGVAERFAGKGGLRGAERFAGAEWRKQVTGVPLLVGALATLDCEVEEIIERHSHAIVIGKVVHVAVAPQSAAL
AYWHGSYVTVDRDDDLGRLAALSVPDPRLTT

Sequences:

>Translated_191_residues
MNSIVRHMPDATALPPDFRSAMRQLVGGVSVITVGRGDEITGMTVSSLSSLAVDPPSIIVSLNRNASSWPILQRERVFGA
NLLAADQIGVAERFAGKGGLRGAERFAGAEWRKQVTGVPLLVGALATLDCEVEEIIERHSHAIVIGKVVHVAVAPQSAAL
AYWHGSYVTVDRDDDLGRLAALSVPDPRLTT
>Mature_191_residues
MNSIVRHMPDATALPPDFRSAMRQLVGGVSVITVGRGDEITGMTVSSLSSLAVDPPSIIVSLNRNASSWPILQRERVFGA
NLLAADQIGVAERFAGKGGLRGAERFAGAEWRKQVTGVPLLVGALATLDCEVEEIIERHSHAIVIGKVVHVAVAPQSAAL
AYWHGSYVTVDRDDDLGRLAALSVPDPRLTT

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=150, Percent_Identity=32, Blast_Score=88, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 20313; Mature: 20313

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIVRHMPDATALPPDFRSAMRQLVGGVSVITVGRGDEITGMTVSSLSSLAVDPPSIIV
CCCHHHHCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCEEHHHHHHHCCCCCEEEE
SLNRNASSWPILQRERVFGANLLAADQIGVAERFAGKGGLRGAERFAGAEWRKQVTGVPL
EECCCCCCCCCHHHHHHCCCCEEECCCCCHHHHHCCCCCCCHHHHHCCHHHHHHHCCCHH
LVGALATLDCEVEEIIERHSHAIVIGKVVHVAVAPQSAALAYWHGSYVTVDRDDDLGRLA
HHHHHHHCCCCHHHHHHHCCCEEEEEEEEEEEECCCCCEEEEECCCEEEEECCCCCCCEE
ALSVPDPRLTT
EECCCCCCCCC
>Mature Secondary Structure
MNSIVRHMPDATALPPDFRSAMRQLVGGVSVITVGRGDEITGMTVSSLSSLAVDPPSIIV
CCCHHHHCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCEEHHHHHHHCCCCCEEEE
SLNRNASSWPILQRERVFGANLLAADQIGVAERFAGKGGLRGAERFAGAEWRKQVTGVPL
EECCCCCCCCCHHHHHHCCCCEEECCCCCHHHHHCCCCCCCHHHHHCCHHHHHHHCCCHH
LVGALATLDCEVEEIIERHSHAIVIGKVVHVAVAPQSAALAYWHGSYVTVDRDDDLGRLA
HHHHHHHCCCCHHHHHHHCCCEEEEEEEEEEEECCCCCEEEEECCCEEEEECCCCCCCEE
ALSVPDPRLTT
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA