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The map label for this gene is ligD [H]

Identifier: 146342656

GI number: 146342656

Start: 6037908

End: 6040622

Strand: Reverse

Name: ligD [H]

Synonym: BRADO5823

Alternate gene names: 146342656

Gene position: 6040622-6037908 (Counterclockwise)

Preceding gene: 146342658

Following gene: 146342654

Centisome position: 81.01

GC content: 64.75

Gene sequence:

>2715_bases
GTGGCGCTCAAGAAGCTTTCCACCTATCGCAAGAAGCGCGATTTCGAAAAAACGGCAGAGCCGTCCGGTGACGCCAGGAT
CGCGCCCGCCGCGCGGCCGCGCTTCGTCATCCAGAAGCATGACGCCACCCGGCTCCATTACGATCTCAGGCTCGAATTCG
ACGGCGTCTTCAAGTCCTGGGCAGTGACCAAGGGGCCGTCGCTCGATCCGCACGACAAGCGCCTCGCGGTTGAGGTCGAG
GATCATCCGCTCGACTATGGCGATTTCGAAGGCACGATTCCTGAAGGCGAATATGGCGGCGGCACGGTGATGCTGTGGGA
CCGCGGCTATTGGGAGAGCGATGATCCCGAACGCGGCTTCACGAAGGGCGACCTGAAATTTACGCTTGAGGGTGAGAAGC
TGCACGGCAGCTGGGTGCTGGTGCGGATGCGCCACGACCGCACCGGCGGCAAGCGCACCAACTGGCTGCTGATCAAGCAT
CGCGACGAGTTCGCCCGGGAAGGCGCGGCCAACGACGTTCTCGATGCCGACAAGTCGGTGGCCTCGGGTCGCACCATGGA
GCAGATCGCGGCCGGCAAGGGCAGGGCACCGAAGCCGTTCATGCTCGCCAAGAGGCAGCGCATGAATGCCGACGCCATAT
GGCACTCCAATCGCAGCGACGGCGAAACGAAGACCAGCAAGGTGTCGACCAAGGCAGCATCGTCGAGAACGAAGGCCGCG
AAGGCAGGGAGGGCGAAGTCCTCGCGCGTGTCGAAGATGGCGGAGCCAAAGACCGTCTCGTCGCTGCCAGACTTCGTGCC
GCCCGAGCTGTGCACCTCCGTCGCACAGCCGCCCAGCGGCGAGGGCTGGGCGCACGAGATCAAGTTCGACGGCTACCGCG
TGCAGATCCGGGTCGAGGATGGCCAGGCGGCGCTGAAGACGCGCAAGGGCCTCGACTGGACCGCCAAGTTCGGCGTGATC
GCCGAGGCGGCTGCCAAGCTGCCGGATGCGATCATCGACGGCGAGATCGTTGCGCTCGACAAGGATGGTAACCCGAACTT
CTCCGCGCTGCAGGCGGCGATCTCGGCCGGGCAGACCGACGAGTTGGTGTTCTTTGCCTTCGACCTGATGTTCGTCGAGG
AGTTCGACCTGCGCCGCCAGCCGCTCCGCGAGCGCAAAGCACGCCTGGCCAAGCTCTTGAAGGCCAACATCAAGGGCAAG
ACGCCCGTGATCCGTTACGTCGAGCATTTCGAGAGCGACGGTGAAGCGGTGCTGGAGTCGGCGCGCAAGCTATCCCTCGA
AGGGATCGTCTCGAAGAAGCTGGACTCGGCCTATCACTCCGGTCGCACCGAGAGCTGGACCAAGGCGAAGACCCGTCCCG
GCCACGAGGTCGTGGTCGGCGGCTGGAAGACCACCAACGGCAAATTCCGCTCGTTGATGGTCGGCGTCAACAAGGACGAT
CATCTCGCTTATGTCGGCATCGTCGGCACCGGCTTCGGTCAGGATACGGTGAAGGCGATCATGCCTGCGCTGAAGGCGGC
CGCATCCGACAAGAGTCCGTTCAGCGGCAAGAATGCCCCGCGCAAGGCGCGCGAGGTGCATTGGCTCAAGCCCGAGCTGG
TCGCCGAGATCGAGTTCGCAGGCTTCACCGGAGACGGCAATGTGAGACAGGCCTCGTTCAAGGGGCTGCGTCAGGACAAG
CCGGCGCGCGAGGTCGTCGCGGAGGAGCCGGTGGTCGCGGCCAAGGTGACGAAGCCGGTGGCGCGGGCCGCCGCCACGGT
CGCGACCGGCGGCACCACGGTCCACAAGACGCCGTCACGCACCAAGGGCGCGGCCACGGTGATGGGCGTTGCGATCTCGA
AGCCGGACAAGGCGCTATGGCCGGATGCGGGCGATGGCGAGCCGGTCACGAAGCTCGATCTCGCCACCTATCTGGAAGCG
GTCGGCGACTGGATGATCGAGCATCTGAAGGGCCGGCCGTGCTCGATCGTCCGTGCGCCCGACGGAATCGGCGGCGAGAG
GTTTTTTCAGCGGCACGGCATGCAGGGCATGTCGGACCTGCTCGAACTGGTAAAGGTGTCCGGAGACCGGAAGCCTTATC
TGCAGATCGATCGCGTTGAGGGACTGATCGCGGTGGCGCAAGTGGCCGCGGTCGAGCTGCATCCGTGGAATTGTGCGCCC
GATGCCTACGATGTGCCGGGCCGCCTGGTGTTCGATCTCGATCCGGCACCCAACGTTGCATTCGAGGAGGTGATCGCGGC
GGCCAAGGAGATGCGGCAGCGTCTCAAGACGCTCGGCCTGGAGAGCTTCTGCAAGACCACCGGCGGCAAGGGCCTGCATG
TCGTGACACCGCTGCTGCATGGCGCGCGCGACCAGGTGACATGGCCGGAGGCGAAAGCGTTTGCGCAGGGCGTGTGCCAG
TGGATGGCCAATGACAGCCCCGACGACTATTTGCTCAACATGTCGAAGAAGCTGCGCAAGGGAAAGATCTTCCTCGACTA
TCTGCGCAACGATCGGATGTCGACGGCGGTTGCCGTGCTGTCGCCGCGGGCGCGCGAGGGCGCGACGGTGTCGATGCCGG
TGACGTGGGCGCAGGTGAAGAACGGACTTGATCCCAAGCGCTACACCATCCGCACCGTACCGGCGCTGCTGGCCAAGACC
AAGGCGTGGGATGGCTACGATGATGCAGCCGGAGCGATCAGGCCGGCAATCAGGAAGCTGACCGACACGTTGTGA

Upstream 100 bases:

>100_bases
CGCTGGGAACGCGGAACTTTGCCCGGTGTTATCATGCGGACCAGCCGGTTTCACGACCTCGCCGGCTTTGCTACGCTACC
GCTCAACGCGAGGGATCACC

Downstream 100 bases:

>100_bases
GAACTCTGAGATCGTTCGGGCAGACGTGGTTTGCGCCACATCTGCCCAACGACGTCATCGCAGGTAGATCAGATCCGTCC
GAGCAGGACAAGGATCAGCA

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 904; Mature: 903

Protein sequence:

>904_residues
MALKKLSTYRKKRDFEKTAEPSGDARIAPAARPRFVIQKHDATRLHYDLRLEFDGVFKSWAVTKGPSLDPHDKRLAVEVE
DHPLDYGDFEGTIPEGEYGGGTVMLWDRGYWESDDPERGFTKGDLKFTLEGEKLHGSWVLVRMRHDRTGGKRTNWLLIKH
RDEFAREGAANDVLDADKSVASGRTMEQIAAGKGRAPKPFMLAKRQRMNADAIWHSNRSDGETKTSKVSTKAASSRTKAA
KAGRAKSSRVSKMAEPKTVSSLPDFVPPELCTSVAQPPSGEGWAHEIKFDGYRVQIRVEDGQAALKTRKGLDWTAKFGVI
AEAAAKLPDAIIDGEIVALDKDGNPNFSALQAAISAGQTDELVFFAFDLMFVEEFDLRRQPLRERKARLAKLLKANIKGK
TPVIRYVEHFESDGEAVLESARKLSLEGIVSKKLDSAYHSGRTESWTKAKTRPGHEVVVGGWKTTNGKFRSLMVGVNKDD
HLAYVGIVGTGFGQDTVKAIMPALKAAASDKSPFSGKNAPRKAREVHWLKPELVAEIEFAGFTGDGNVRQASFKGLRQDK
PAREVVAEEPVVAAKVTKPVARAAATVATGGTTVHKTPSRTKGAATVMGVAISKPDKALWPDAGDGEPVTKLDLATYLEA
VGDWMIEHLKGRPCSIVRAPDGIGGERFFQRHGMQGMSDLLELVKVSGDRKPYLQIDRVEGLIAVAQVAAVELHPWNCAP
DAYDVPGRLVFDLDPAPNVAFEEVIAAAKEMRQRLKTLGLESFCKTTGGKGLHVVTPLLHGARDQVTWPEAKAFAQGVCQ
WMANDSPDDYLLNMSKKLRKGKIFLDYLRNDRMSTAVAVLSPRAREGATVSMPVTWAQVKNGLDPKRYTIRTVPALLAKT
KAWDGYDDAAGAIRPAIRKLTDTL

Sequences:

>Translated_904_residues
MALKKLSTYRKKRDFEKTAEPSGDARIAPAARPRFVIQKHDATRLHYDLRLEFDGVFKSWAVTKGPSLDPHDKRLAVEVE
DHPLDYGDFEGTIPEGEYGGGTVMLWDRGYWESDDPERGFTKGDLKFTLEGEKLHGSWVLVRMRHDRTGGKRTNWLLIKH
RDEFAREGAANDVLDADKSVASGRTMEQIAAGKGRAPKPFMLAKRQRMNADAIWHSNRSDGETKTSKVSTKAASSRTKAA
KAGRAKSSRVSKMAEPKTVSSLPDFVPPELCTSVAQPPSGEGWAHEIKFDGYRVQIRVEDGQAALKTRKGLDWTAKFGVI
AEAAAKLPDAIIDGEIVALDKDGNPNFSALQAAISAGQTDELVFFAFDLMFVEEFDLRRQPLRERKARLAKLLKANIKGK
TPVIRYVEHFESDGEAVLESARKLSLEGIVSKKLDSAYHSGRTESWTKAKTRPGHEVVVGGWKTTNGKFRSLMVGVNKDD
HLAYVGIVGTGFGQDTVKAIMPALKAAASDKSPFSGKNAPRKAREVHWLKPELVAEIEFAGFTGDGNVRQASFKGLRQDK
PAREVVAEEPVVAAKVTKPVARAAATVATGGTTVHKTPSRTKGAATVMGVAISKPDKALWPDAGDGEPVTKLDLATYLEA
VGDWMIEHLKGRPCSIVRAPDGIGGERFFQRHGMQGMSDLLELVKVSGDRKPYLQIDRVEGLIAVAQVAAVELHPWNCAP
DAYDVPGRLVFDLDPAPNVAFEEVIAAAKEMRQRLKTLGLESFCKTTGGKGLHVVTPLLHGARDQVTWPEAKAFAQGVCQ
WMANDSPDDYLLNMSKKLRKGKIFLDYLRNDRMSTAVAVLSPRAREGATVSMPVTWAQVKNGLDPKRYTIRTVPALLAKT
KAWDGYDDAAGAIRPAIRKLTDTL
>Mature_903_residues
ALKKLSTYRKKRDFEKTAEPSGDARIAPAARPRFVIQKHDATRLHYDLRLEFDGVFKSWAVTKGPSLDPHDKRLAVEVED
HPLDYGDFEGTIPEGEYGGGTVMLWDRGYWESDDPERGFTKGDLKFTLEGEKLHGSWVLVRMRHDRTGGKRTNWLLIKHR
DEFAREGAANDVLDADKSVASGRTMEQIAAGKGRAPKPFMLAKRQRMNADAIWHSNRSDGETKTSKVSTKAASSRTKAAK
AGRAKSSRVSKMAEPKTVSSLPDFVPPELCTSVAQPPSGEGWAHEIKFDGYRVQIRVEDGQAALKTRKGLDWTAKFGVIA
EAAAKLPDAIIDGEIVALDKDGNPNFSALQAAISAGQTDELVFFAFDLMFVEEFDLRRQPLRERKARLAKLLKANIKGKT
PVIRYVEHFESDGEAVLESARKLSLEGIVSKKLDSAYHSGRTESWTKAKTRPGHEVVVGGWKTTNGKFRSLMVGVNKDDH
LAYVGIVGTGFGQDTVKAIMPALKAAASDKSPFSGKNAPRKAREVHWLKPELVAEIEFAGFTGDGNVRQASFKGLRQDKP
AREVVAEEPVVAAKVTKPVARAAATVATGGTTVHKTPSRTKGAATVMGVAISKPDKALWPDAGDGEPVTKLDLATYLEAV
GDWMIEHLKGRPCSIVRAPDGIGGERFFQRHGMQGMSDLLELVKVSGDRKPYLQIDRVEGLIAVAQVAAVELHPWNCAPD
AYDVPGRLVFDLDPAPNVAFEEVIAAAKEMRQRLKTLGLESFCKTTGGKGLHVVTPLLHGARDQVTWPEAKAFAQGVCQW
MANDSPDDYLLNMSKKLRKGKIFLDYLRNDRMSTAVAVLSPRAREGATVSMPVTWAQVKNGLDPKRYTIRTVPALLAKTK
AWDGYDDAAGAIRPAIRKLTDTL

Specific function: Probably involved in the repair of DNA double-strand breaks by non-homologous-end joining (NHEJ) during spore germination [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR014146
- InterPro:   IPR014145
- InterPro:   IPR014143 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 99184; Mature: 99053

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKKLSTYRKKRDFEKTAEPSGDARIAPAARPRFVIQKHDATRLHYDLRLEFDGVFKSW
CCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCEEEEEEEEEEECCHHHHH
AVTKGPSLDPHDKRLAVEVEDHPLDYGDFEGTIPEGEYGGGTVMLWDRGYWESDDPERGF
EECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TKGDLKFTLEGEKLHGSWVLVRMRHDRTGGKRTNWLLIKHRDEFAREGAANDVLDADKSV
CCCCEEEEEECCEECCCEEEEEEECCCCCCCCCEEEEEEECHHHHHCCCCCCHHHCCHHH
ASGRTMEQIAAGKGRAPKPFMLAKRQRMNADAIWHSNRSDGETKTSKVSTKAASSRTKAA
HCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHH
KAGRAKSSRVSKMAEPKTVSSLPDFVPPELCTSVAQPPSGEGWAHEIKFDGYRVQIRVED
HHCCCHHHHHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCEEEEEEECC
GQAALKTRKGLDWTAKFGVIAEAAAKLPDAIIDGEIVALDKDGNPNFSALQAAISAGQTD
CCHHHHHHCCCCCHHHHHHHHHHHHHCCCHHCCCEEEEEECCCCCCHHHHHHHHHCCCCC
ELVFFAFDLMFVEEFDLRRQPLRERKARLAKLLKANIKGKTPVIRYVEHFESDGEAVLES
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHH
ARKLSLEGIVSKKLDSAYHSGRTESWTKAKTRPGHEVVVGGWKTTNGKFRSLMVGVNKDD
HHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCEEEEECEECCCCCEEEEEEECCCCC
HLAYVGIVGTGFGQDTVKAIMPALKAAASDKSPFSGKNAPRKAREVHWLKPELVAEIEFA
CEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHEEEEEEE
GFTGDGNVRQASFKGLRQDKPAREVVAEEPVVAAKVTKPVARAAATVATGGTTVHKTPSR
EECCCCCEEHHHHHCCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCEEECCCCC
TKGAATVMGVAISKPDKALWPDAGDGEPVTKLDLATYLEAVGDWMIEHLKGRPCSIVRAP
CCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DGIGGERFFQRHGMQGMSDLLELVKVSGDRKPYLQIDRVEGLIAVAQVAAVELHPWNCAP
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHEEEEECCCCCC
DAYDVPGRLVFDLDPAPNVAFEEVIAAAKEMRQRLKTLGLESFCKTTGGKGLHVVTPLLH
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEHHHHHC
GARDQVTWPEAKAFAQGVCQWMANDSPDDYLLNMSKKLRKGKIFLDYLRNDRMSTAVAVL
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEHHHCCCHHHHHHEE
SPRAREGATVSMPVTWAQVKNGLDPKRYTIRTVPALLAKTKAWDGYDDAAGAIRPAIRKL
CCCCCCCCEEEECEEHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
TDTL
HHCC
>Mature Secondary Structure 
ALKKLSTYRKKRDFEKTAEPSGDARIAPAARPRFVIQKHDATRLHYDLRLEFDGVFKSW
CHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCEEEEEEEEEEECCHHHHH
AVTKGPSLDPHDKRLAVEVEDHPLDYGDFEGTIPEGEYGGGTVMLWDRGYWESDDPERGF
EECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TKGDLKFTLEGEKLHGSWVLVRMRHDRTGGKRTNWLLIKHRDEFAREGAANDVLDADKSV
CCCCEEEEEECCEECCCEEEEEEECCCCCCCCCEEEEEEECHHHHHCCCCCCHHHCCHHH
ASGRTMEQIAAGKGRAPKPFMLAKRQRMNADAIWHSNRSDGETKTSKVSTKAASSRTKAA
HCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHH
KAGRAKSSRVSKMAEPKTVSSLPDFVPPELCTSVAQPPSGEGWAHEIKFDGYRVQIRVED
HHCCCHHHHHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCEEEEEEECC
GQAALKTRKGLDWTAKFGVIAEAAAKLPDAIIDGEIVALDKDGNPNFSALQAAISAGQTD
CCHHHHHHCCCCCHHHHHHHHHHHHHCCCHHCCCEEEEEECCCCCCHHHHHHHHHCCCCC
ELVFFAFDLMFVEEFDLRRQPLRERKARLAKLLKANIKGKTPVIRYVEHFESDGEAVLES
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHH
ARKLSLEGIVSKKLDSAYHSGRTESWTKAKTRPGHEVVVGGWKTTNGKFRSLMVGVNKDD
HHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCEEEEECEECCCCCEEEEEEECCCCC
HLAYVGIVGTGFGQDTVKAIMPALKAAASDKSPFSGKNAPRKAREVHWLKPELVAEIEFA
CEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHEEEEEEE
GFTGDGNVRQASFKGLRQDKPAREVVAEEPVVAAKVTKPVARAAATVATGGTTVHKTPSR
EECCCCCEEHHHHHCCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCEEECCCCC
TKGAATVMGVAISKPDKALWPDAGDGEPVTKLDLATYLEAVGDWMIEHLKGRPCSIVRAP
CCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DGIGGERFFQRHGMQGMSDLLELVKVSGDRKPYLQIDRVEGLIAVAQVAAVELHPWNCAP
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHEEEEECCCCCC
DAYDVPGRLVFDLDPAPNVAFEEVIAAAKEMRQRLKTLGLESFCKTTGGKGLHVVTPLLH
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEHHHHHC
GARDQVTWPEAKAFAQGVCQWMANDSPDDYLLNMSKKLRKGKIFLDYLRNDRMSTAVAVL
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEHHHCCCHHHHHHEE
SPRAREGATVSMPVTWAQVKNGLDPKRYTIRTVPALLAKTKAWDGYDDAAGAIRPAIRKL
CCCCCCCCEEEECEEHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
TDTL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]