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The map label for this gene is ywtE [H]

Identifier: 146342646

GI number: 146342646

Start: 6020126

End: 6020938

Strand: Direct

Name: ywtE [H]

Synonym: BRADO5813

Alternate gene names: 146342646

Gene position: 6020126-6020938 (Clockwise)

Preceding gene: 146342645

Following gene: 146342649

Centisome position: 80.74

GC content: 66.67

Gene sequence:

>813_bases
ATGACCCGCATCGCTCTCGTCGTGTCCGATGTCGACGGCACGCTGCTGACCAAGGACAAGGTCCTCACCGAGCGCGCGGC
CAAGGCCGCGCGCGAGCTGCGCGAGGCCGGGATCGCCTTCACCATCACCTCGAGCCGCCCGGCGATCGGCATGGGTTTCC
TGATCGCGCCGTTGCAGATCGATCTGCCGGTCGGTCCCTTCAATGGCAGCTCGATCGTCAGTCCCGAAATGCGGCCGCTC
GAGCAGAGCCTGATTCCGGCGGACGCCGCCGAGCGCTCGGTTGACCTGCTGCATCAGCGCGGCATCGACATCTGGCTGTT
CACCGCCGACGCCTGGCTGACGCGCAATCCGGACGGCGACTATGTGCCGAACGAGCAGCGCGCCATCCGCGCCGACCCCA
CCATCATCGGCGATTTCGCGCCCTATCTGTCGAGCGCCTGCAAGATCGTCGGCGCCAGCCGCGACTTCGATCTGCTCGCC
CGCTGCGAAGCCGAGATGCGCGAACTGCTGGGCAGCGAGGCCACCGCGGTCCGCTCGCAGAGCTACTATCTCGACATCAC
CCCGCCCGGCCGCAACAAGGGCACCTTCGTCACCGCGATGGCGCGGCGGCTCGGAATTGCCCTGGACCAGGTGGCGACCA
TCGGCGACATGCACAATGACGTCGCCATGTTCGAGGTGAGCGGCCTGTCGATCGCGATGGGCAATGCCAGCGACGACGTG
AAAAGCAAGGCGAGGCGCGTTACGACCTCCAACCAGGACGAGGGCTTTGCCAACGCCATGGAGATGATCCTCGCCGAGAA
CGCGCAGGGCTAG

Upstream 100 bases:

>100_bases
CAAGGTCTCGGTCAACGCGCCGGTCGAGCATATCGTCGACGACGTCCTGCAACAGCTTGCAATCGGTCGGGACCGTGCCT
GACCACGAATGGAAGGGCTC

Downstream 100 bases:

>100_bases
CGCTCGGAGTCACGCATCCGAGCGCTGCTTGGCGGGCTTGTCGCACGTCCTTTTGGCGCCGCTGAGATTCTGCGCCGTGT
TGACCAAGGCGATATGCGTC

Product: putative Cof hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTRIALVVSDVDGTLLTKDKVLTERAAKAARELREAGIAFTITSSRPAIGMGFLIAPLQIDLPVGPFNGSSIVSPEMRPL
EQSLIPADAAERSVDLLHQRGIDIWLFTADAWLTRNPDGDYVPNEQRAIRADPTIIGDFAPYLSSACKIVGASRDFDLLA
RCEAEMRELLGSEATAVRSQSYYLDITPPGRNKGTFVTAMARRLGIALDQVATIGDMHNDVAMFEVSGLSIAMGNASDDV
KSKARRVTTSNQDEGFANAMEMILAENAQG

Sequences:

>Translated_270_residues
MTRIALVVSDVDGTLLTKDKVLTERAAKAARELREAGIAFTITSSRPAIGMGFLIAPLQIDLPVGPFNGSSIVSPEMRPL
EQSLIPADAAERSVDLLHQRGIDIWLFTADAWLTRNPDGDYVPNEQRAIRADPTIIGDFAPYLSSACKIVGASRDFDLLA
RCEAEMRELLGSEATAVRSQSYYLDITPPGRNKGTFVTAMARRLGIALDQVATIGDMHNDVAMFEVSGLSIAMGNASDDV
KSKARRVTTSNQDEGFANAMEMILAENAQG
>Mature_269_residues
TRIALVVSDVDGTLLTKDKVLTERAAKAARELREAGIAFTITSSRPAIGMGFLIAPLQIDLPVGPFNGSSIVSPEMRPLE
QSLIPADAAERSVDLLHQRGIDIWLFTADAWLTRNPDGDYVPNEQRAIRADPTIIGDFAPYLSSACKIVGASRDFDLLAR
CEAEMRELLGSEATAVRSQSYYLDITPPGRNKGTFVTAMARRLGIALDQVATIGDMHNDVAMFEVSGLSIAMGNASDDVK
SKARRVTTSNQDEGFANAMEMILAENAQG

Specific function: Unknown

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI1786982, Length=263, Percent_Identity=26.615969581749, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081741, Length=246, Percent_Identity=30.0813008130081, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI2367265, Length=267, Percent_Identity=27.3408239700375, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 29145; Mature: 29014

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIALVVSDVDGTLLTKDKVLTERAAKAARELREAGIAFTITSSRPAIGMGFLIAPLQI
CCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEEEE
DLPVGPFNGSSIVSPEMRPLEQSLIPADAAERSVDLLHQRGIDIWLFTADAWLTRNPDGD
ECCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCEEEEECCCCC
YVPNEQRAIRADPTIIGDFAPYLSSACKIVGASRDFDLLARCEAEMRELLGSEATAVRSQ
CCCCCCCEEECCCCEEEHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCHHEEECC
SYYLDITPPGRNKGTFVTAMARRLGIALDQVATIGDMHNDVAMFEVSGLSIAMGNASDDV
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCHHH
KSKARRVTTSNQDEGFANAMEMILAENAQG
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRIALVVSDVDGTLLTKDKVLTERAAKAARELREAGIAFTITSSRPAIGMGFLIAPLQI
CEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEEEE
DLPVGPFNGSSIVSPEMRPLEQSLIPADAAERSVDLLHQRGIDIWLFTADAWLTRNPDGD
ECCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCEEEEECCCCC
YVPNEQRAIRADPTIIGDFAPYLSSACKIVGASRDFDLLARCEAEMRELLGSEATAVRSQ
CCCCCCCEEECCCCEEEHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCHHEEECC
SYYLDITPPGRNKGTFVTAMARRLGIALDQVATIGDMHNDVAMFEVSGLSIAMGNASDDV
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCHHH
KSKARRVTTSNQDEGFANAMEMILAENAQG
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]