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The map label for this gene is thcR [H]

Identifier: 146342606

GI number: 146342606

Start: 5972155

End: 5973204

Strand: Reverse

Name: thcR [H]

Synonym: BRADO5770

Alternate gene names: 146342606

Gene position: 5973204-5972155 (Counterclockwise)

Preceding gene: 146342610

Following gene: 146342604

Centisome position: 80.11

GC content: 66.95

Gene sequence:

>1050_bases
ATGGCGACGTCTTCAGCCGCTGATCTCAGTTGGAAAACATCCTCATCGCCGCGGCTCGCCGCCTACGGCCGTGTTGCAAC
GCACGATGTCGATGAGGCGGCTGAACAAATAGGTCGCATCTTCTGTCCGCACGGGCTGCAGCCGCAGGCCGGGACTGCGC
GCGATTTCCTCGCTCTGCATAATTGCGCGGCATACGACGGCTTCTCCATCAACTACGTCGCATATGGCGGCGCGGTCGCG
ATCAATCCCGGCTGTCTCGATCGCTTCTTCCTGGTGCAACTGCCGCTGGCAGGGACGGCGCGGGTCATGACTGGCGGTCG
CGAGGTCGTGTCTCACCCGGAGCAGGCGGCATCACTGCTGTCTCCGACCTTGCCGACGCGCATGGTGTGGCACGACTGCG
CCCAGTTGATCCTGCTGCTCGATCGCAGCCTGGTCGAGCAGCGCGCCGCGGCGCTGTCCGGGCAGGCGGTGCAGCCTGTC
GAATTCCAGCCCGAGATCGCGCTATCGGCGCCGGCGGGTTCACGGCTCCAGCAGCGCATGATCTCGCTTGCTGGCGTGGC
CGAGAGCCTCGGTGGCCAAGGGCGCATCTCGGTCGCGGCGGCAGCGGATTGGCGCGAAACGCTGCTGGATGCGCTTTTCG
AGCAGTCGGGCCACGGCCTGTCGGAGGCCATCCGGCGCTATGCGGGCCAGATCGACCATCTGCCGCGGGCGGTGCGCACG
GCGCGCGATCTGCTGCATGCGCAGGCCAGCGAGCCGCTCGATCTGGCGCAGCTTGCAGCTGCTGCGGGCGTCGGCATCCG
CGCGCTGCAAGGCGGCTTCCGACGCCATTTCGGCATGTCGATCTCGGACATGCTGCTGGATATCAGGTTGGCCAGGTTGA
ACGCGCAACTGACGGCGGCCGGCCCCGAGACGCGCATCATCGACCTCGCTTTCGAGCTCGGCTTCACCCATCTCGGACGG
ATGGCGGGCGCCTATCGCGACAAGTTCGGAGAGACGCCGTCAGCCACGCTGCAGCGCGTGCAGCGTGGCCGAGGTCCGCT
CACGAACTGA

Upstream 100 bases:

>100_bases
CGAGCAGATCGCGCCGCGGTGTCTATCCAGAAAACGAATCTCTCGACGCCAGCTGCTAATTATTATAGGTTTCAAACGAT
TTCGACGGATGATGTGAGCC

Downstream 100 bases:

>100_bases
CCTGACGAACTGACCTGACGAACTGACTTCACGAATTGACGTGGACGAAATCGCGCAGCAGCGGATAGATCTCGTTGTTC
CACTTCTTGCCGCTGAACAC

Product: AraC/XylS family transcription factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MATSSAADLSWKTSSSPRLAAYGRVATHDVDEAAEQIGRIFCPHGLQPQAGTARDFLALHNCAAYDGFSINYVAYGGAVA
INPGCLDRFFLVQLPLAGTARVMTGGREVVSHPEQAASLLSPTLPTRMVWHDCAQLILLLDRSLVEQRAAALSGQAVQPV
EFQPEIALSAPAGSRLQQRMISLAGVAESLGGQGRISVAAAADWRETLLDALFEQSGHGLSEAIRRYAGQIDHLPRAVRT
ARDLLHAQASEPLDLAQLAAAAGVGIRALQGGFRRHFGMSISDMLLDIRLARLNAQLTAAGPETRIIDLAFELGFTHLGR
MAGAYRDKFGETPSATLQRVQRGRGPLTN

Sequences:

>Translated_349_residues
MATSSAADLSWKTSSSPRLAAYGRVATHDVDEAAEQIGRIFCPHGLQPQAGTARDFLALHNCAAYDGFSINYVAYGGAVA
INPGCLDRFFLVQLPLAGTARVMTGGREVVSHPEQAASLLSPTLPTRMVWHDCAQLILLLDRSLVEQRAAALSGQAVQPV
EFQPEIALSAPAGSRLQQRMISLAGVAESLGGQGRISVAAAADWRETLLDALFEQSGHGLSEAIRRYAGQIDHLPRAVRT
ARDLLHAQASEPLDLAQLAAAAGVGIRALQGGFRRHFGMSISDMLLDIRLARLNAQLTAAGPETRIIDLAFELGFTHLGR
MAGAYRDKFGETPSATLQRVQRGRGPLTN
>Mature_348_residues
ATSSAADLSWKTSSSPRLAAYGRVATHDVDEAAEQIGRIFCPHGLQPQAGTARDFLALHNCAAYDGFSINYVAYGGAVAI
NPGCLDRFFLVQLPLAGTARVMTGGREVVSHPEQAASLLSPTLPTRMVWHDCAQLILLLDRSLVEQRAAALSGQAVQPVE
FQPEIALSAPAGSRLQQRMISLAGVAESLGGQGRISVAAAADWRETLLDALFEQSGHGLSEAIRRYAGQIDHLPRAVRTA
RDLLHAQASEPLDLAQLAAAAGVGIRALQGGFRRHFGMSISDMLLDIRLARLNAQLTAAGPETRIIDLAFELGFTHLGRM
AGAYRDKFGETPSATLQRVQRGRGPLTN

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 37279; Mature: 37148

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSSAADLSWKTSSSPRLAAYGRVATHDVDEAAEQIGRIFCPHGLQPQAGTARDFLALH
CCCCCCCCCCCCCCCCCCEECCCCHHHCCHHHHHHHHHHEECCCCCCCCCCCHHHHHHHH
NCAAYDGFSINYVAYGGAVAINPGCLDRFFLVQLPLAGTARVMTGGREVVSHPEQAASLL
HHHCCCCCEEEEEEECCEEEECCHHHHHHHHHCCCCCCCHHHHCCCHHHHCCHHHHHHHH
SPTLPTRMVWHDCAQLILLLDRSLVEQRAAALSGQAVQPVEFQPEIALSAPAGSRLQQRM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHH
ISLAGVAESLGGQGRISVAAAADWRETLLDALFEQSGHGLSEAIRRYAGQIDHLPRAVRT
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ARDLLHAQASEPLDLAQLAAAAGVGIRALQGGFRRHFGMSISDMLLDIRLARLNAQLTAA
HHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEC
GPETRIIDLAFELGFTHLGRMAGAYRDKFGETPSATLQRVQRGRGPLTN
CCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ATSSAADLSWKTSSSPRLAAYGRVATHDVDEAAEQIGRIFCPHGLQPQAGTARDFLALH
CCCCCCCCCCCCCCCCCEECCCCHHHCCHHHHHHHHHHEECCCCCCCCCCCHHHHHHHH
NCAAYDGFSINYVAYGGAVAINPGCLDRFFLVQLPLAGTARVMTGGREVVSHPEQAASLL
HHHCCCCCEEEEEEECCEEEECCHHHHHHHHHCCCCCCCHHHHCCCHHHHCCHHHHHHHH
SPTLPTRMVWHDCAQLILLLDRSLVEQRAAALSGQAVQPVEFQPEIALSAPAGSRLQQRM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHH
ISLAGVAESLGGQGRISVAAAADWRETLLDALFEQSGHGLSEAIRRYAGQIDHLPRAVRT
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ARDLLHAQASEPLDLAQLAAAAGVGIRALQGGFRRHFGMSISDMLLDIRLARLNAQLTAA
HHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEC
GPETRIIDLAFELGFTHLGRMAGAYRDKFGETPSATLQRVQRGRGPLTN
CCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]